Abstract: In-vitro mouse co-culture of E14 embryonic stem cells
(ESCs) and OP9 stromal cells can recapitulate the earliest stages of
haematopoietic development, not accessible in human embryos,
supporting both haemogenic precursors and their primitive
haematopoietic progeny. 1α, 25-Dihydroxy-vitamin D3 (VD3) has
been demonstrated to be a powerful differentiation inducer for a wide
variety of neoplastic cells, and could enhance early differentiation of
ESCs into blood cells in E14/OP9 co-culture. This study aims to
ascertain whether VD3 is key in promoting differentiation and
suppressing proliferation, by separately investigating the effects of
VD3 on the proliferation phase of the E14 cell line and on stromal
OP9 cells.The results showed that VD3 inhibited the proliferation of
the cells in a dose-dependent manner, quantitatively by decreased cell
number, and qualitatively by alkaline-phosphatase staining that
revealed significant differences between VD3-treated and untreated
cells, characterised by decreased enzyme expression (colourless
cells). Propidium-iodide cell-cycle analyses showed no significant
percentage change in VD3-treated E14 and OP9 cells within their G
and S-phases, compared to the untreated controls, despite the
increased percentage of G-phase compared to the S-phase in a dosedependent
manner. These results with E14 and OP9 cells indicate that
adequate VD3 concentration enhances cellular differentiation and
inhibits proliferation. The results also suggest that if E14 and OP9
cells were co-cultured andVD3-treated, there would be furtherenhanced
differentiation of ESCs into blood cells.
Abstract: Bionanotechnology deals with nanoscopic interactions between nanostructured materials and biological systems. Polymer nanocomposites with optimized biological activity have attracted great attention. Nanoclay is considered as reinforcing nanofiller in manufacturing of high performance nanocomposites. In current study, organomodified-nanoclay with negatively charged silicate layers was incorporated into biomedical grade silicone rubber. Nanoparticle loading has been tailored to enhance cell behavior. Addition of nanoparticles led to improved mechanical properties of substrate with enhanced strength and stiffness while no toxic effects was observed. Results indicated improved viability and proliferation of cells by addition of nanofillers. The improved mechanical properties of the matrix result in proper cell response through adjustment and arrangement of cytoskeletal fibers. Results can be applied in tissue engineering when enhanced substrates are required for improvement of cell behavior for in vivo applications.
Abstract: The massive proliferation of affordable computers, Internet broadband connectivity and rich education content has created a global phenomenon in which information and communication technology (ICT) is being used to transform education. Therefore, there is a need to redesign the educational system to meet the needs better. The advent of computers with sophisticated software has made it possible to solve many complex problems very fast and at a lower cost. This paper introduces the characteristics of the current E-Learning and then analyses the concept of cloud computing and describes the architecture of cloud computing platform by combining the features of E-Learning. The authors have tried to introduce cloud computing to e-learning, build an e-learning cloud, and make an active research and exploration for it from the following aspects: architecture, construction method and external interface with the model.
Abstract: With the proliferation of multi-channel retailing, developing a better understanding of the factors that affect customers- purchase behaviors within a multi-channel retail context has become an important topic for practitioners and academics. While many studies have investigated the various customer behaviors associated with brick-and-mortar retailing, online retailing, and brick-and-click retailing, little research has explored how customer shopping value perceptions influence online purchase behaviors within the TV-and-online retail environment. The main purpose of this study is to investigate the influence of TV and online shopping values on online patronage intention. Data collected from 116 respondents in Taiwan are tested against the research model using the partial least squares (PLS) approach. The results indicate that utilitarian and hedonic TV shopping values have indirect, positive influences on online patronage intention through their online counterparts in the TV-and-online retail context. The findings of this study provide several important theoretical and practical implications for multi-channel retailing.
Abstract: A systems approach model for prostate cancer in prostate duct, as a sub-system of the organism is developed. It is accomplished in two steps. First this research work starts with a nonlinear system of coupled Fokker-Plank equations which models continuous process of the system like motion of cells. Then extended to PDEs that include discontinuous processes like cell mutations, proliferation and deaths. The discontinuous processes is modeled by using intensity poisson processes. The model incorporates the features of the prostate duct. The system of PDEs spatial coordinate is along the proximal distal axis. Its parameters depend on features of the prostate duct. The movement of cells is biased towards distal region and mutations of prostate cancer cells is localized in the proximal region. Numerical solutions of the full system of equations are provided, and are exhibit traveling wave fronts phenomena. This motivates the use of the standard transformation to derive a canonically related system of ODEs for traveling wave solutions. The results obtained show persistence of prostate cancer by showing that the non-negative cone for the traveling wave system is time invariant. The traveling waves have a unique global attractor is proved also. Biologically, the global attractor verifies that evolution of prostate cancer stem cells exhibit the avascular tumor growth. These numerical solutions show that altering prostate stem cell movement or mutation of prostate cancer cells lead to avascular tumor. Conclusion with comments on clinical implications of the model is discussed.
Abstract: Goat milk has an hypoallergenic effects, and allergic
diseases related to abnormal of intestinal flora. Probiotic microorganisms
do exert an activity on the immune system in the skin of
the individual.The purpose of this study are to determine the number
of leukocyte and lymphocyte proliferation in rat supplemented with
fermented goat milk (acidophilus milk and kefir) and sensitized with
dinitrochlorobenzene (DNCB). Female Wistar rats 6-8 weeks olds
were divided into 3 treatment groups. The first group supplemented
goat milk kefir, second group acidophilus goat milk, and third group
as control. During 28-day experiment, on day 15 rat sensitized with
allergen DNCB on the dorsal of the body, and on day 24 was
challenged with DNCB on the ear. Sampling of blood and tissue of
intestinal Peyer'patch (PP) were performed on day 14 (before DNCB
sensitized) and on day 28 (after DNCB sensitized). The results
showed the number of neutrophils in rats supplemented with
acidophilus milk was higher (P