Abstract: A new reverse phase-high performance liquid chromatography (RP-HPLC) method with fluorescent detector (FLD) was developed and optimized for Norfloxacin determination in human plasma. Mobile phase specifications, extraction method and excitation and emission wavelengths were varied for optimization. HPLC system contained a reverse phase C18 (5 μm, 4.6 mm×150 mm) column with FLD operated at excitation 330 nm and emission 440 nm. The optimized mobile phase consisted of 14% acetonitrile in buffer solution. The aqueous phase was prepared by mixing 2g of citric acid, 2g sodium acetate and 1 ml of triethylamine in 1 L of Milli-Q water was run at a flow rate of 1.2 mL/min. The standard curve was linear for the range tested (0.156–20 μg/mL) and the coefficient of determination was 0.9978. Aceclofenac sodium was used as internal standard. A detection limit of 0.078 μg/mL was achieved. Run time was set at 10 minutes because retention time of norfloxacin was 0.99 min. which shows the rapidness of this method of analysis. The present assay showed good accuracy, precision and sensitivity for Norfloxacin determination in human plasma with a new internal standard and can be applied pharmacokinetic evaluation of Norfloxacin tablets after oral administration in human.
Abstract: The development of the power electronics has allowed
increasing the precision and reliability of the electrical trainings,
thanks to the adjustable inverters, as the Pulse Wide Modulation
(PWM) five level inverters, which is the object of study in this
article.The authors treat the relation between the law order adopted for
a given system and the oscillations of the electrical and mechanical
parameters of which the tolerance depends on the process with which
they are integrated (paper factory, lifting of the heavy loads,
etc.).Thus the best choice of the regulation indexes allows us to
achieve stability and safety training without investment (management
of existing equipment).
Abstract: Machine tools are improved capacity remarkably during the 20th century. Improving the precision of machine tools are related with precision of products and accurate processing is always associated with the subject of interest. There are a lot of the elements that determine the precision of the machine, as guides, motors, structure, control, etc. In this paper we focused on the phenomenon that vertical movement system has worse precision than horizontal movement system even they were made up with same components. The vertical movement system needs to be studied differently from the horizontal movement system to develop its precision. The vertical movement system has load on its transfer direction and it makes the movement system weak in precision than the horizontal one. Some machines have mechanical counter balance, hydraulic or pneumatic counter balance to compensate the weight of the machine head. And there is several type of compensating the weight. It can push the machine head and also can use chain or wire lope to transfer the compensating force from counter balance to machine head. According to the type of compensating, there could be error from friction, pressure error of hydraulic or pressure control error. Also according to what to use for transferring the compensating force, transfer error of compensating force could be occur.
Abstract: Microaneurysm is a key indicator of diabetic retinopathy that can potentially cause damage to retina. Early detection and automatic quantification are the keys to prevent further damage. In this paper, which focuses on automatic microaneurysm detection in images acquired through non-dilated pupils, we present a series of experiments on feature selection and automatic microaneurysm pixel classification. We found that the best feature set is a combination of 10 features: the pixel-s intensity of shade corrected image, the pixel hue, the standard deviation of shade corrected image, DoG4, the area of the candidate MA, the perimeter of the candidate MA, the eccentricity of the candidate MA, the circularity of the candidate MA, the mean intensity of the candidate MA on shade corrected image and the ratio of the major axis length and minor length of the candidate MA. The overall sensitivity, specificity, precision, and accuracy are 84.82%, 99.99%, 89.01%, and 99.99%, respectively.
Abstract: Numerical studies on race car aerodynamics at wing
in ground effect have been carried out using a steady 3d, double
precision, pressure-based, and standard k-epsilon turbulence model.
Through various parametric analytical studies we have observed that
at a particular speed and ground clearance of the wings a favorable
negative lift was found high at a particular angle of attack for all the
physical models considered in this paper. The fact is that if the
ground clearance height to chord length (h/c) is too small, the
developing boundary layers from either side (the ground and the
lower surface of the wing) can interact, leading to an altered variation
of the aerodynamic characteristics at wing in ground effect. Therefore
a suitable ground clearance must be predicted throughout the racing
for a better performance of the race car, which obviously depends
upon the coupled effects of the topography, wing orientation with
respect to the ground, the incoming flow features and/or the race car
speed. We have concluded that for the design of high performance
and high speed race cars the adjustable wings capable to alter the
ground clearance and the angles of attack is the best design option for
any race car for racing safely with variable speeds.
Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: The method of gait identification based on the nearest neighbor classification technique with motion similarity assessment by the dynamic time warping is proposed. The model based kinematic motion data, represented by the joints rotations coded by Euler angles and unit quaternions is used. The different pose distance functions in Euler angles and quaternion spaces are considered. To evaluate individual features of the subsequent joints movements during gait cycle, joint selection is carried out. To examine proposed approach database containing 353 gaits of 25 humans collected in motion capture laboratory is used. The obtained results are promising. The classifications, which takes into consideration all joints has accuracy over 91%. Only analysis of movements of hip joints allows to correctly identify gaits with almost 80% precision.
Abstract: The quality of short term load forecasting can improve the efficiency of planning and operation of electric utilities. Artificial Neural Networks (ANNs) are employed for nonlinear short term load forecasting owing to their powerful nonlinear mapping capabilities. At present, there is no systematic methodology for optimal design and training of an artificial neural network. One has often to resort to the trial and error approach. This paper describes the process of developing three layer feed-forward large neural networks for short-term load forecasting and then presents a heuristic search algorithm for performing an important task of this process, i.e. optimal networks structure design. Particle Swarm Optimization (PSO) is used to develop the optimum large neural network structure and connecting weights for one-day ahead electric load forecasting problem. PSO is a novel random optimization method based on swarm intelligence, which has more powerful ability of global optimization. Employing PSO algorithms on the design and training of ANNs allows the ANN architecture and parameters to be easily optimized. The proposed method is applied to STLF of the local utility. Data are clustered due to the differences in their characteristics. Special days are extracted from the normal training sets and handled separately. In this way, a solution is provided for all load types, including working days and weekends and special days. The experimental results show that the proposed method optimized by PSO can quicken the learning speed of the network and improve the forecasting precision compared with the conventional Back Propagation (BP) method. Moreover, it is not only simple to calculate, but also practical and effective. Also, it provides a greater degree of accuracy in many cases and gives lower percent errors all the time for STLF problem compared to BP method. Thus, it can be applied to automatically design an optimal load forecaster based on historical data.
Abstract: In this paper a computer system for electromagnetic
properties measurements is designed. The system employs Agilent
4294A precision impedance analyzer to measure the amplitude and
the phase of a signal applied over a tested biological tissue sample.
Measured by the developed computer system data could be used for
tissue characterization in wide frequency range from 40Hz to
110MHz. The computer system can interface with output devices
acquiring flexible testing process.
Abstract: Chatter vibration has been a troublesome problem
for a machine tool toward the high precision and high speed machining.
Essentially, the machining performance is determined by the dynamic
characteristics of the machine tool structure and dynamics of cutting
process, which can further be identified in terms of the stability lobe
diagram. Therefore, realization on the machine tool dynamic behavior
can help to enhance the cutting stability. To assess the dynamic
characteristics and machining stability of a vertical milling system
under the influence of a linear guide, this study developed a finite
element model integrated the modeling of linear components with the
implementation of contact stiffness at the rolling interface. Both the
finite element simulations and experimental measurements reveal that
the linear guide with different preload greatly affects the vibration
behavior and milling stability of the vertical column spindle head
system, which also clearly indicate that the predictions of the
machining stability agree well with the cutting tests. It is believed that
the proposed model can be successfully applied to evaluate the
dynamics performance of machine tool systems of various
configurations.
Abstract: Harmonic pollution and low power factor in power
systems caused by power converters have been of great concern. To
overcome these problems several converter topologies using
advanced semiconductor devices and control schemes have been
proposed. This investigation is to identify a low cost, small size,
efficient and reliable ac to dc converter to meet the input performance
index of UPS. The performance of single phase and three phase ac to
dc converter along with various control techniques are studied and
compared. The half bridge converter topology with linear current
control is identified as most suitable. It is simple, energy efficient
because of single switch power loss and transformer-less operation of
UPS. The results are validated practically using a prototype built
using IGBT and analog controller. The performance for both single
and three-phase system is verified. Digital implementation of closed
loop control achieves higher reliability. Its cost largely depends on
chosen bit precision. The minimal bit precision for optimum
converter performance is identified as 16-bit with fixed-point
operation. From the investigation and practical implementation it is
concluded that half bridge ac – dc converter along with digital linear
controller meets the performance index of UPS for single and three
phase systems.
Abstract: This paper presents software tools that convert the C/Cµ floating point source code for a DSP algorithm into a fixedpoint simulation model that can be used to evaluate the numericalperformance of the algorithm on several different fixed pointplatforms including microprocessors, DSPs and FPGAs. The tools use a novel system for maintaining binary point informationso that the conversion from floating point to fixed point isautomated and the resulting fixed point algorithm achieves maximum possible precision. A configurable architecture is used during the simulation phase so that the algorithm can produce a bit-exact output for several different target devices.
Abstract: Precise frequency estimation methods for pulseshaped echoes are a prerequisite to determine the relative velocity between sensor and reflector. Signal frequencies are analysed using three different methods: Fourier Transform, Chirp ZTransform and the MUSIC algorithm. Simulations of echoes are performed varying both the noise level and the number of reflecting points. The superposition of echoes with a random initial phase is found to influence the precision of frequency estimation severely for FFT and MUSIC. The standard deviation of the frequency using FFT is larger than for MUSIC. However, MUSIC is more noise-sensitive. The distorting effect of superpositions is less pronounced in experimental data.
Abstract: Music Information Retrieval (MIR) and modern data mining techniques are applied to identify style markers in midi music for stylometric analysis and author attribution. Over 100 attributes are extracted from a library of 2830 songs then mined using supervised learning data mining techniques. Two attributes are identified that provide high informational gain. These attributes are then used as style markers to predict authorship. Using these style markers the authors are able to correctly distinguish songs written by the Beatles from those that were not with a precision and accuracy of over 98 per cent. The identification of these style markers as well as the architecture for this research provides a foundation for future research in musical stylometry.
Abstract: In the recent past, there has been an increasing interest
in applying evolutionary methods to Knowledge Discovery in
Databases (KDD) and a number of successful applications of Genetic
Algorithms (GA) and Genetic Programming (GP) to KDD have been
demonstrated. The most predominant representation of the
discovered knowledge is the standard Production Rules (PRs) in the
form If P Then D. The PRs, however, are unable to handle
exceptions and do not exhibit variable precision. The Censored
Production Rules (CPRs), an extension of PRs, were proposed by
Michalski & Winston that exhibit variable precision and supports an
efficient mechanism for handling exceptions. A CPR is an
augmented production rule of the form:
If P Then D Unless C, where C (Censor) is an exception to the rule.
Such rules are employed in situations, in which the conditional
statement 'If P Then D' holds frequently and the assertion C holds
rarely. By using a rule of this type we are free to ignore the exception
conditions, when the resources needed to establish its presence are
tight or there is simply no information available as to whether it
holds or not. Thus, the 'If P Then D' part of the CPR expresses
important information, while the Unless C part acts only as a switch
and changes the polarity of D to ~D.
This paper presents a classification algorithm based on evolutionary
approach that discovers comprehensible rules with exceptions in the
form of CPRs.
The proposed approach has flexible chromosome encoding, where
each chromosome corresponds to a CPR. Appropriate genetic
operators are suggested and a fitness function is proposed that
incorporates the basic constraints on CPRs. Experimental results are
presented to demonstrate the performance of the proposed algorithm.
Abstract: Need for an appropriate system of evaluating students-
educational developments is a key problem to achieve the predefined
educational goals. Intensity of the related papers in the last years; that
tries to proof or disproof the necessity and adequacy of the students
assessment; is the corroborator of this matter. Some of these studies
tried to increase the precision of determining question weights in
scientific examinations. But in all of them there has been an attempt
to adjust the initial question weights while the accuracy and precision
of those initial question weights are still under question. Thus In
order to increase the precision of the assessment process of students-
educational development, the present study tries to propose a new
method for determining the initial question weights by considering
the factors of questions like: difficulty, importance and complexity;
and implementing a combined method of PROMETHEE and fuzzy
analytic network process using a data mining approach to improve
the model-s inputs. The result of the implemented case study proves
the development of performance and precision of the proposed
model.
Abstract: This paper studies the optimum design for reducing
optical loss of an 8x8 mechanical type optical switch due to the
temperature change. The 8x8 optical switch is composed of a base, 8
input fibers, 8 output fibers, 3 fixed mirrors and 17 movable mirrors.
First, an innovative switch configuration is proposed with
thermal-compensated design. Most mechanical type optical switches
have a disadvantage that their precision and accuracy are influenced
by the ambient temperature. Therefore, the thermal-compensated
design is to deal with this situation by using materials with different
thermal expansion coefficients (α). Second, a parametric modeling
program is developed to generate solid models for finite element
analysis, and the thermal and structural behaviors of the switch are
analyzed. Finally, an integrated optimum design program, combining
Autodesk Inventor Professional software, finite element analysis
software, and genetic algorithms, is developed for improving the
thermal behaviors that the optical loss of the switch is reduced. By
changing design parameters of the switch in the integrated design
program, the final optimum design that satisfies the design constraints
and specifications can be found.
Abstract: The aim of the present study was to develop and
validate an inexpensive and simple high performance liquid
chromatographic (HPLC) method for the determination of colistin
sulfate. Separation of colistin sulfate was achieved on a ZORBAX
Eclipse XDB-C18 column using UV detection at λ=215 nm. The
mobile phase was 30 mM sulfate buffer (pH 2.5):acetonitrile(76:24).
An excellent linearity (r2=0.998) was found in the concentration
range of 25 - 400 μg/mL. Intra- day and inter-day precisions of
method (%RSD, n=3) were less than 7.9%.The developed and
validated method was applied to determination of the content of
colistin sulfate in medicated premix and animal feed sample.The
recovery of colistin from animal feed was satisfactorily ranged from
90.92 to 93.77%. The results demonstrated that the HPLC method
developed in this work is appropriate for direct determination of
colistin sulfate in commercial medicated premixes and animal feed.
Abstract: One of the important applications of gas turbines is
their utilization for heat recovery steam generator in combine-cycle technology. Exhaust flow and energy are two key parameters for
determining heat recovery steam generator performance which are mainly determined by the main gas turbine components performance
data. For this reason a method was developed for determining the
exhaust energy in the new edition of ASME PTC22. The result of this investigation shows that the method of standard has considerable
error. Therefore in this paper a new method is presented for modifying of the performance calculation. The modified method is
based on exhaust gas constituent analysis and combustion calculations. The case study presented here by two kind of General
Electric gas turbine design data for validation of methodologies. The
result shows that the modified method is more precise than the ASME PTC22 method. The exhaust flow calculation deviation from
design data is 1.5-2 % by ASME PTC22 method so that the deviation regarding with modified method is 0.3-0.5%. Based on precision of
analyzer instruments, the method can be suitable alternative for gas
turbine standard performance test. In advance two methods are
proposed based on known and unknown fuel in modified method procedure. The result of this paper shows that the difference between
the two methods is below than %0.02. In according to reasonable esult of the second procedure (unknown fuel composition), the
method can be applied to performance evaluation of gas turbine, so that the measuring cost and data gathering should be reduced.
Abstract: Camera calibration is an important step in 3D
reconstruction. Camera calibration may be classified into two major types: traditional calibration and self-calibration. However, a calibration method in using a checkerboard is intermediate between traditional calibration and self-calibration. A self
is proposed based on a square in this paper. Only a square in the planar
template, the camera self-calibration can be completed through the single view. The proposed algorithm is that the virtual circle and straight line are established by a square on planar template, and
circular points, vanishing points in straight lines and the relation
between them are be used, in order to obtain the image of the absolute
conic (IAC) and establish the camera intrinsic parameters. To make
the calibration template is simpler, as compared with the Zhang Zhengyou-s method. Through real experiments and experiments, the experimental results show that this algorithm is
feasible and available, and has a certain precision and robustness.