High Performance Liquid Chromatographic Method for Determination of Colistin Sulfate and its Application in Medicated Premixand Animal Feed
The aim of the present study was to develop and
validate an inexpensive and simple high performance liquid
chromatographic (HPLC) method for the determination of colistin
sulfate. Separation of colistin sulfate was achieved on a ZORBAX
Eclipse XDB-C18 column using UV detection at λ=215 nm. The
mobile phase was 30 mM sulfate buffer (pH 2.5):acetonitrile(76:24).
An excellent linearity (r2=0.998) was found in the concentration
range of 25 - 400 μg/mL. Intra- day and inter-day precisions of
method (%RSD, n=3) were less than 7.9%.The developed and
validated method was applied to determination of the content of
colistin sulfate in medicated premix and animal feed sample.The
recovery of colistin from animal feed was satisfactorily ranged from
90.92 to 93.77%. The results demonstrated that the HPLC method
developed in this work is appropriate for direct determination of
colistin sulfate in commercial medicated premixes and animal feed.
[1] J. Orwa, A. Van Gerven, E. Roets, and J. Hoogmartens, "Development
and validation of a liquid chromatography method for analysis of
colistin sulphate," Chromatographia, vol 51, pp. 433-436, 2000.
[2] Y. Ikai, H. Oka, J. Hayakawa, N. Kawamura, K Harada, M.Suzuki,
H.Nakazawa and Y. Ito, Journal of Liquid Chromatography and Related
Technologies, vol 21, pp.143, 1998
[3] A.R. Martin, J.L. Delgado, and W.A. Remers (Eds.), Wilson and
Gisvold-s Textbook of Organic Medicinal and Pharmaceutical
Chemistry, 10th ed., 1998.
[4] Y. Xu, X.i Tian, C. Ren, H. Huang, X. Zhang, X. Gong, H. Liu, Z. Yu,
and L. Zhang, "Analysis of colistin A and B in fishery products by ultra
performance liquid chromatography with positive electrospray ionization
tandem mass spectrometry," Journal of Chromatography B, vol 899,pp.
14-20, 2012.
[5] The notification of the Department of Livestock Development (No.2/2)
, " the maximum residue limits of animal product", Department of
Livestock Development, Bangkok, Thailand, May, 2001.
[6] S. Morales-Munoz and M. D. Luque de Castro, "Dynamic ultrasoundassisted
extraction of colistin from feeds with on-line pre-column
derivatization and liquid chromatography-fluorimetric detection,"
Journal of Chromatography A, vol 1066, pp. 1-7, 2005.
[7] P. Leroy, D. Decolin, S. Nicolas, P. Archimbault and A. Nicolas,
"Residue determination of two co-administered antibacterial agents
cephalexin and colistin in calf tissues using high-performance liquid
chromatography and microbiological methods.," Journal of
Pharmaceutical and Biomedical Analysis, vol 7, pp.1837-1846, 1989.
[8] T. Suzuki, H. Inouye, K. Fujikawa and Y. Suketa, "Studies on the
Chemical Structure of Colistin: I. Fractionation, Molecular Weight
Determination, Amino Acid and Fatty Acid Composition," Journal of
Biochemistry, vol 54, pp.25-33 ,1963.
[9] A. H. Thomas and L. Holloway, "Thin-layer chromatographic method
for the identification of the polymyxins," Journal of Chromatography A,
vol 161, pp.417-420, 1978.
[10] W. Holska and E. Gwozdz.,"Determination of colistin by
isotachophoresis," Journal of Liquid Chromatography, vol 12, pp. 2761-
67, 1989.
[11] H. K. Kristensen, and S. H. Hansen, "Separation of Polymyxins by
Micellar Electrokinetic Capillary Chromatography," Journal of
Chromatography, vol. 628, pp. 309-315, 1993.
[12] B. Cancho Grande, M. Garc├¡a Falc├│n, C. Pérez-Lamela, M. Rodr├¡guez
Comesaña, and J. Simal Gándara, "Quantitative analysis of colistin and
tiamulin in liquid and solid medicated premixes by HPLC with diodearray
detection," Chromatographia, vol 53, pp. 460-463, 2001.
[13] J. Li, R. W. Milne, R. L. Nation, J.D. Turnidge, K. Coulthard, and D. W.
Johnson, "A simple method for the assay of colistin in human plasma,
using pre-column derivatization with 9-fluorenylmethyl chloroformate
in solid-phase extraction cartridges and reversed-phase highperformance
liquid chromatography," Journal of Chromatography B:
Biomedical Sciences and Applications, vol 761, pp.167-175, 2001.
[14] C. Cheng, S. Liu, D. Xiao, J. Hollembaek, L. Yao, J. Lin, and S. Hansel,
"LC-MS/MS method development and validation for the determination
of polymyxins and vancomycin in rat plasma," Journal of
Chromatography B, vol 878, pp.2831-2838, 2010.
[15] D. W. Sin, E. C. Wan, C. Ho, and Y. C. Wong., "Detection of residual
bacitracin A, colistin A, and colistin B in milk and animal tissues by
liquid chromatography tandem mass spectrometry," Analytical and
Bioanalytical Chemistry , vol 385, pp.181-188, 2006.
[1] J. Orwa, A. Van Gerven, E. Roets, and J. Hoogmartens, "Development
and validation of a liquid chromatography method for analysis of
colistin sulphate," Chromatographia, vol 51, pp. 433-436, 2000.
[2] Y. Ikai, H. Oka, J. Hayakawa, N. Kawamura, K Harada, M.Suzuki,
H.Nakazawa and Y. Ito, Journal of Liquid Chromatography and Related
Technologies, vol 21, pp.143, 1998
[3] A.R. Martin, J.L. Delgado, and W.A. Remers (Eds.), Wilson and
Gisvold-s Textbook of Organic Medicinal and Pharmaceutical
Chemistry, 10th ed., 1998.
[4] Y. Xu, X.i Tian, C. Ren, H. Huang, X. Zhang, X. Gong, H. Liu, Z. Yu,
and L. Zhang, "Analysis of colistin A and B in fishery products by ultra
performance liquid chromatography with positive electrospray ionization
tandem mass spectrometry," Journal of Chromatography B, vol 899,pp.
14-20, 2012.
[5] The notification of the Department of Livestock Development (No.2/2)
, " the maximum residue limits of animal product", Department of
Livestock Development, Bangkok, Thailand, May, 2001.
[6] S. Morales-Munoz and M. D. Luque de Castro, "Dynamic ultrasoundassisted
extraction of colistin from feeds with on-line pre-column
derivatization and liquid chromatography-fluorimetric detection,"
Journal of Chromatography A, vol 1066, pp. 1-7, 2005.
[7] P. Leroy, D. Decolin, S. Nicolas, P. Archimbault and A. Nicolas,
"Residue determination of two co-administered antibacterial agents
cephalexin and colistin in calf tissues using high-performance liquid
chromatography and microbiological methods.," Journal of
Pharmaceutical and Biomedical Analysis, vol 7, pp.1837-1846, 1989.
[8] T. Suzuki, H. Inouye, K. Fujikawa and Y. Suketa, "Studies on the
Chemical Structure of Colistin: I. Fractionation, Molecular Weight
Determination, Amino Acid and Fatty Acid Composition," Journal of
Biochemistry, vol 54, pp.25-33 ,1963.
[9] A. H. Thomas and L. Holloway, "Thin-layer chromatographic method
for the identification of the polymyxins," Journal of Chromatography A,
vol 161, pp.417-420, 1978.
[10] W. Holska and E. Gwozdz.,"Determination of colistin by
isotachophoresis," Journal of Liquid Chromatography, vol 12, pp. 2761-
67, 1989.
[11] H. K. Kristensen, and S. H. Hansen, "Separation of Polymyxins by
Micellar Electrokinetic Capillary Chromatography," Journal of
Chromatography, vol. 628, pp. 309-315, 1993.
[12] B. Cancho Grande, M. Garc├¡a Falc├│n, C. Pérez-Lamela, M. Rodr├¡guez
Comesaña, and J. Simal Gándara, "Quantitative analysis of colistin and
tiamulin in liquid and solid medicated premixes by HPLC with diodearray
detection," Chromatographia, vol 53, pp. 460-463, 2001.
[13] J. Li, R. W. Milne, R. L. Nation, J.D. Turnidge, K. Coulthard, and D. W.
Johnson, "A simple method for the assay of colistin in human plasma,
using pre-column derivatization with 9-fluorenylmethyl chloroformate
in solid-phase extraction cartridges and reversed-phase highperformance
liquid chromatography," Journal of Chromatography B:
Biomedical Sciences and Applications, vol 761, pp.167-175, 2001.
[14] C. Cheng, S. Liu, D. Xiao, J. Hollembaek, L. Yao, J. Lin, and S. Hansel,
"LC-MS/MS method development and validation for the determination
of polymyxins and vancomycin in rat plasma," Journal of
Chromatography B, vol 878, pp.2831-2838, 2010.
[15] D. W. Sin, E. C. Wan, C. Ho, and Y. C. Wong., "Detection of residual
bacitracin A, colistin A, and colistin B in milk and animal tissues by
liquid chromatography tandem mass spectrometry," Analytical and
Bioanalytical Chemistry , vol 385, pp.181-188, 2006.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:63022", author = "S.Choosakoonkriang and S. Supaluknari and P. Puangkaew", title = "High Performance Liquid Chromatographic Method for Determination of Colistin Sulfate and its Application in Medicated Premixand Animal Feed", abstract = "The aim of the present study was to develop and
validate an inexpensive and simple high performance liquid
chromatographic (HPLC) method for the determination of colistin
sulfate. Separation of colistin sulfate was achieved on a ZORBAX
Eclipse XDB-C18 column using UV detection at λ=215 nm. The
mobile phase was 30 mM sulfate buffer (pH 2.5):acetonitrile(76:24).
An excellent linearity (r2=0.998) was found in the concentration
range of 25 - 400 μg/mL. Intra- day and inter-day precisions of
method (%RSD, n=3) were less than 7.9%.The developed and
validated method was applied to determination of the content of
colistin sulfate in medicated premix and animal feed sample.The
recovery of colistin from animal feed was satisfactorily ranged from
90.92 to 93.77%. The results demonstrated that the HPLC method
developed in this work is appropriate for direct determination of
colistin sulfate in commercial medicated premixes and animal feed.", keywords = "Colistin sulfate, HPLC,medicated premix, animal feed", volume = "7", number = "4", pages = "217-5", }
