Detection of Diabetic Symptoms in Retina Images Using Analog Algorithms

In this paper a class of analog algorithms based on the concept of Cellular Neural Network (CNN) is applied in some processing operations of some important medical images, namely retina images, for detecting various symptoms connected with diabetic retinopathy. Some specific processing tasks like morphological operations, linear filtering and thresholding are proposed, the corresponding template values are given and simulations on real retina images are provided.

MITAutomatic ECG Beat Tachycardia Detection Using Artificial Neural Network

The application of Neural Network for disease diagnosis has made great progress and is widely used by physicians. An Electrocardiogram carries vital information about heart activity and physicians use this signal for cardiac disease diagnosis which was the great motivation towards our study. In our work, tachycardia features obtained are used for the training and testing of a Neural Network. In this study we are using Fuzzy Probabilistic Neural Networks as an automatic technique for ECG signal analysis. As every real signal recorded by the equipment can have different artifacts, we needed to do some preprocessing steps before feeding it to our system. Wavelet transform is used for extracting the morphological parameters of the ECG signal. The outcome of the approach for the variety of arrhythmias shows the represented approach is superior than prior presented algorithms with an average accuracy of about %95 for more than 7 tachy arrhythmias.

Generalized Morphological 3D Shape Decomposition Grayscale Interframe Interpolation Method

One of the main image representations in Mathematical Morphology is the 3D Shape Decomposition Representation, useful for Image Compression and Representation,and Pattern Recognition. The 3D Morphological Shape Decomposition representation can be generalized a number of times,to extend the scope of its algebraic characteristics as much as possible. With these generalizations, the Morphological Shape Decomposition 's role to serve as an efficient image decomposition tool is extended to grayscale images.This work follows the above line, and further develops it. Anew evolutionary branch is added to the 3D Morphological Shape Decomposition's development, by the introduction of a 3D Multi Structuring Element Morphological Shape Decomposition, which permits 3D Morphological Shape Decomposition of 3D binary images (grayscale images) into "multiparameter" families of elements. At the beginning, 3D Morphological Shape Decomposition representations are based only on "1 parameter" families of elements for image decomposition.This paper addresses the gray scale inter frame interpolation by means of mathematical morphology. The new interframe interpolation method is based on generalized morphological 3D Shape Decomposition. This article will present the theoretical background of the morphological interframe interpolation, deduce the new representation and show some application examples.Computer simulations could illustrate results.

Cytotoxic Effects of Engineered Nanoparticles in Human Mesenchymal Stem Cells

Engineered nanoparticles’ usage rapidly increased in various applications in the last decade due to their unusual properties. However, there is an ever increasing concern to understand their toxicological effect in human health. Particularly, metal and metal oxide nanoparticles have been used in various sectors including biomedical, food and agriculture. But their impact on human health is yet to be fully understood. In this present investigation, we assessed the toxic effect of engineered nanoparticles (ENPs) including Ag, MgO and Co3O4 nanoparticles (NPs) on human mesenchymal stem cells (hMSC) adopting cell viability and cellular morphological changes as tools The results suggested that silver NPs are more toxic than MgO and Co3O4NPs. The ENPs induced cytotoxicity and nuclear morphological changes in hMSC depending on dose. The cell viability decreases with increase in concentration of ENPs. The cellular morphology studies revealed that ENPs damaged the cells. These preliminary findings have implications for the use of these nanoparticles in food industry with systematic regulations.

Ethnobotany and Distribution of Wild Edible Tubers in Pulau Redang and Nearby Islands of Terengganu, Malaysia

An ethnobotanical study was conducted to document local knowledge and potentials of wild edible tubers that has been reported and sighted and to investigate and record their distribution in Pulau Redang and nearby islands of Terengganu, Malaysia. Information was gathered from 42 villagers by using semi-structured questionnaire. These respondents were selected randomly and no appointment was made prior to the visits. For distribution, the locations of wild edible tubers were recorded by using the Global Positioning System (GPS). The wild edible tubers recorded were ubi gadung, ubi toyo, ubi kasu, ubi jaga, ubi seratus and ubi kertas. Dioscorea or commonly known as yam is reported to be one of the major food sources worldwide. The majority of villagers used Dioscorea hispida Dennst. or ubi gadung in many ways in their life such as for food, medicinal purposes and fish poison. The villagers have identified this ubi gadung by looking at the morphological characteristics; that include leaf shape, stem and the color of the tuber-s flesh.

Influence of Thermal Annealing on The Structural Properties of Vanadyl Phthalocyanine Thin Films: A Comparative Study

This paper presents a comparative study on Vanadyl Phthalocyanine (VOPc) thin films deposited by thermal evaporation and spin coating techniques. The samples were prepared on cleaned glass substrates and annealed at various temperatures ranging form 95oC to 155oC. To obtain the morphological and structural properties of VOPc thin films, X-ray diffraction (XRD) technique and atomic force microscopy (AFM) have been implied. The AFM topographic images show a very slight difference in the thermally grown films, before and after annealing, however best results are achieved for the spin-cast film annealed at 125oC. The XRD spectra show no existence of the sharp peaks, suggesting the material to be amorphous. The humps in the XRD patterns indicate the presence of some crystallites.

Superior Performances of the Neural Network on the Masses Lesions Classification through Morphological Lesion Differences

Purpose of this work is to develop an automatic classification system that could be useful for radiologists in the breast cancer investigation. The software has been designed in the framework of the MAGIC-5 collaboration. In an automatic classification system the suspicious regions with high probability to include a lesion are extracted from the image as regions of interest (ROIs). Each ROI is characterized by some features based generally on morphological lesion differences. A study in the space features representation is made and some classifiers are tested to distinguish the pathological regions from the healthy ones. The results provided in terms of sensitivity and specificity will be presented through the ROC (Receiver Operating Characteristic) curves. In particular the best performances are obtained with the Neural Networks in comparison with the K-Nearest Neighbours and the Support Vector Machine: The Radial Basis Function supply the best results with 0.89 ± 0.01 of area under ROC curve but similar results are obtained with the Probabilistic Neural Network and a Multi Layer Perceptron.

Fuzzy Mathematical Morphology approach in Image Processing

Morphological operators transform the original image into another image through the interaction with the other image of certain shape and size which is known as the structure element. Mathematical morphology provides a systematic approach to analyze the geometric characteristics of signals or images, and has been applied widely too many applications such as edge detection, objection segmentation, noise suppression and so on. Fuzzy Mathematical Morphology aims to extend the binary morphological operators to grey-level images. In order to define the basic morphological operations such as fuzzy erosion, dilation, opening and closing, a general method based upon fuzzy implication and inclusion grade operators is introduced. The fuzzy morphological operations extend the ordinary morphological operations by using fuzzy sets where for fuzzy sets, the union operation is replaced by a maximum operation, and the intersection operation is replaced by a minimum operation. In this work, it consists of two articles. In the first one, fuzzy set theory, fuzzy Mathematical morphology which is based on fuzzy logic and fuzzy set theory; fuzzy Mathematical operations and their properties will be studied in details. As a second part, the application of fuzziness in Mathematical morphology in practical work such as image processing will be discussed with the illustration problems.

Screening and Identification of Microorganisms – Potential Producers of Arachidonic Acid

Microorganisms isolated from water and soil of Kazakhstan to identify potential high-effective producers of the arachidonic acid, exhibiting a wide range of physiological activity and having practical applications were screened. Based on the results of two independent tests (the test on the sensitivity of the growth processes of microorganisms to acetylsalicylic acid - an irreversible inhibitor of PGH-synthase involved in the metabolism of arachidonic acid and its derivatives, the test for inhibition of peroxidase activity of membrane-bounding fraction of PGH - synthase by acetylsalicylic acid) were selected microbial cultures which are potential highproducer of arachidonic acid. They are characterized by a stable strong growth in the laboratory conditions. Identification of microorganism cultures based on morphological, physiological, biochemical and molecular genetic characteristics was performed.

Properties of Composite Nanofiber Produced by Single and Coaxial Nozzle Method used for Electrospinning Technique

In this study, single nozzle method used for electrospinning technique which composite polymer solution with cellulose nanowiskers (CNW) was treated by ultrasonic sonificator have been compared with coaxial (double) nozzle method, in terms of mechanical, thermal and morphological properties of composite nanofiber. The effect of water content in composite polymer solution on properties of nanofiber has also been examined. It has been seen that single nozzle method which polymer solution does not contain water has better results than that of coaxial method, in terms of mechanical, thermal and morphological properties of nanofiber. However, it is necessary to make an optimization study on setting condition of ultrasonic treatment to get better dispersion of CNW in composite nanofiber and to get better mechanical and thermal properties

External Morphological Study of Wild Labeo calbasu with Reference to Body Weight' Total Length and Condition Factor from the River Chenab, Punjab, Pakistan

115 samples of Labeo calbasu ranged 8.0-17.9cm length with mean11.90±1.96 and 4.9-68.5g weight with mean 22.25±12.54 from the River Chenab, Southern Punjab, Pakistan were analyzed to investigate length-weight relationships (LWR) of fish in relation to condition factor (K). Standard length (SL), fork length (FL), head length (HL) head width (HW), body girth (BG), dorsal fin length (DFL), dorsal fin base (DFB), pectoral fin length (PcFL), pelvic fin length (PvFL) and anal fin length (AFL) are found to be highly correlated with increasing total length and wet body weight (r > 0.500). Wet body weight has positive (r=0.540) and total length has no correlation (r=0.344) with calculated Condition factor (K). The slope “b" in the relationship is 3.27 and intercepts -2.2258.

High Resolution Images: Segmenting, Extracting Information and GIS Integration

As the world changes more rapidly, the demand for update information for resource management, environment monitoring, planning are increasing exponentially. Integration of Remote Sensing with GIS technology will significantly promote the ability for addressing these concerns. This paper presents an alternative way of update GIS applications using image processing and high resolution images. We show a method of high-resolution image segmentation using graphs and morphological operations, where a preprocessing step (watershed operation) is required. A morphological process is then applied using the opening and closing operations. After this segmentation we can extract significant cartographic elements such as urban areas, streets or green areas. The result of this segmentation and this extraction is then used to update GIS applications. Some examples are shown using aerial photography.

A Trainable Neural Network Ensemble for ECG Beat Classification

This paper illustrates the use of a combined neural network model for classification of electrocardiogram (ECG) beats. We present a trainable neural network ensemble approach to develop customized electrocardiogram beat classifier in an effort to further improve the performance of ECG processing and to offer individualized health care. We process a three stage technique for detection of premature ventricular contraction (PVC) from normal beats and other heart diseases. This method includes a denoising, a feature extraction and a classification. At first we investigate the application of stationary wavelet transform (SWT) for noise reduction of the electrocardiogram (ECG) signals. Then feature extraction module extracts 10 ECG morphological features and one timing interval feature. Then a number of multilayer perceptrons (MLPs) neural networks with different topologies are designed. The performance of the different combination methods as well as the efficiency of the whole system is presented. Among them, Stacked Generalization as a proposed trainable combined neural network model possesses the highest recognition rate of around 95%. Therefore, this network proves to be a suitable candidate in ECG signal diagnosis systems. ECG samples attributing to the different ECG beat types were extracted from the MIT-BIH arrhythmia database for the study.

Extracting Road Signs using the Color Information

In this paper, we propose a method to extract the road signs. Firstly, the grabbed image is converted into the HSV color space to detect the road signs. Secondly, the morphological operations are used to reduce noise. Finally, extract the road sign using the geometric property. The feature extraction of road sign is done by using the color information. The proposed method has been tested for the real situations. From the experimental results, it is seen that the proposed method can extract the road sign features effectively.

Feasibility Investigation of Near Infrared Spectrometry for Particle Size Estimation of Nano Structures

Determination of nano particle size is substantial since the nano particle size exerts a significant effect on various properties of nano materials. Accordingly, proposing non-destructive, accurate and rapid techniques for this aim is of high interest. There are some conventional techniques to investigate the morphology and grain size of nano particles such as scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffractometry (XRD). Vibrational spectroscopy is utilized to characterize different compounds and applied for evaluation of the average particle size based on relationship between particle size and near infrared spectra [1,4] , but it has never been applied in quantitative morphological analysis of nano materials. So far, the potential application of nearinfrared (NIR) spectroscopy with its ability in rapid analysis of powdered materials with minimal sample preparation, has been suggested for particle size determination of powdered pharmaceuticals. The relationship between particle size and diffuse reflectance (DR) spectra in near infrared region has been applied to introduce a method for estimation of particle size. Back propagation artificial neural network (BP-ANN) as a nonlinear model was applied to estimate average particle size based on near infrared diffuse reflectance spectra. Thirty five different nano TiO2 samples with different particle size were analyzed by DR-FTNIR spectrometry and the obtained data were processed by BP- ANN.

A Fuzzy Tumor Volume Estimation Approach Based On Fuzzy Segmentation of MR Images

Quantitative measurements of tumor in general and tumor volume in particular, become more realistic with the use of Magnetic Resonance imaging, especially when the tumor morphological changes become irregular and difficult to assess by clinical examination. However, tumor volume estimation strongly depends on the image segmentation, which is fuzzy by nature. In this paper a fuzzy approach is presented for tumor volume segmentation based on the fuzzy connectedness algorithm. The fuzzy affinity matrix resulting from segmentation is then used to estimate a fuzzy volume based on a certainty parameter, an Alpha Cut, defined by the user. The proposed method was shown to highly affect treatment decisions. A statistical analysis was performed in this study to validate the results based on a manual method for volume estimation and the importance of using the Alpha Cut is further explained.

Physical and Chemical Investigation of Polycaprolactone, Nanohydroxyapatite and Poly (Vinyl Alcohol) Nanocomposite Scaffolds

Aligned and random nanofibrous scaffolds of PVA/PCL/nHA were fabricated by electrospinning method. The composite nanofibrous scaffolds were subjected to detailed analysis. Morphological investigations revealed that the prepared nanofibers have uniform morphology and the average fiber diameters of aligned and random scaffolds were 135.5 and 290 nm, respectively. The obtained scaffolds have a porous structure with porosity of 88 and 76% for random and aligned nanofibers, respectively. Furthermore, FTIR analysis demonstrated that there were strong intramolecular interactions between the molecules of PVA/PCL/nHA. On the other hand, mechanical characterizations show that aligning the nanofibers, could significantly improve the rigidity of the resultant biocomposite nanofibrous scaffolds.

White Blood Cells Identification and Counting from Microscopic Blood Image

The counting and analysis of blood cells allows the evaluation and diagnosis of a vast number of diseases. In particular, the analysis of white blood cells (WBCs) is a topic of great interest to hematologists. Nowadays the morphological analysis of blood cells is performed manually by skilled operators. This involves numerous drawbacks, such as slowness of the analysis and a nonstandard accuracy, dependent on the operator skills. In literature there are only few examples of automated systems in order to analyze the white blood cells, most of which only partial. This paper presents a complete and fully automatic method for white blood cells identification from microscopic images. The proposed method firstly individuates white blood cells from which, subsequently, nucleus and cytoplasm are extracted. The whole work has been developed using MATLAB environment, in particular the Image Processing Toolbox.

Real-time Haptic Modeling and Simulation for Prosthetic Insertion

In this work a surgical simulator is produced which enables a training otologist to conduct a virtual, real-time prosthetic insertion. The simulator provides the Ear, Nose and Throat surgeon with real-time visual and haptic responses during virtual cochlear implantation into a 3D model of the human Scala Tympani (ST). The parametric model is derived from measured data as published in the literature and accounts for human morphological variance, such as differences in cochlear shape, enabling patient-specific pre- operative assessment. Haptic modeling techniques use real physical data and insertion force measurements, to develop a force model which mimics the physical behavior of an implant as it collides with the ST walls during an insertion. Output force profiles are acquired from the insertion studies conducted in the work, to validate the haptic model. The simulator provides the user with real-time, quantitative insertion force information and associated electrode position as user inserts the virtual implant into the ST model. The information provided by this study may also be of use to implant manufacturers for design enhancements as well as for training specialists in optimal force administration, using the simulator. The paper reports on the methods for anatomical modeling and haptic algorithm development, with focus on simulator design, development, optimization and validation. The techniques may be transferrable to other medical applications that involve prosthetic device insertions where user vision is obstructed.

Genetic Variation of Durum Wheat Landraces and Cultivars Using Morphological and Protein Markers

Knowledge of patterns of genetic diversity enhances the efficiency of germplasm conservation and improvement. In this study 96 Iranian landraces of Triticum turgidum originating from different geographical areas of Iran, along with 18 durum cultivars from ten countries were evaluated for variation in morphological and high molecular weight glutenin subunit (HMW-GS) composition. The first two principal components clearly separated the Iranian landraces from cultivars. Three alleles were present at the Glu-A1 locus and 11 alleles at Glu-B1. In both cultivars and landraces of durum wheat, the null allele (Glu-A1c) was observed more frequently than the Glu-A1a and Glu-A1b alleles. Two alleles, namely Glu-B1a (subunit 7) and Glu-B1e (subunit 20) represented the more frequent alleles at Glu-B1 locus. The results showed that the evaluated Iranian landraces formed an interesting source of favourable glutenin subunits that might be very desirable in breeding activities for improving pasta-making quality.