Abstract: Particle detection in very noisy and low contrast images
is an active field of research in image processing. In this article, a
method is proposed for the efficient detection and sizing of subsurface
spherical particles, which is used for the processing of softly fused
Au nanoparticles. Transmission Electron Microscopy is used for
imaging the nanoparticles, and the proposed algorithm has been
tested with the two-dimensional projected TEM images obtained.
Results are compared with the data obtained by transmission optical
spectroscopy, as well as with conventional circular object detection
algorithms.
Abstract: Cancer becomes one of the leading cause of death in
many countries over the world. Fourier-transform infrared (FTIR)
spectra of human lung cancer cells (A549) treated with PMF (natural
product extracted from PM 701) for different time intervals were
examined. Second derivative and difference method were taken in
comparison studies. Cesium (Cs) and Rubidium (Rb) nanoparticles in
PMF were detected by Energy Dispersive X-ray attached to Scanning
Electron Microscope SEM-EDX. Characteristic changes in protein
secondary structure, lipid profile and changes in the intensities of
DNA bands were identified in treated A549 cells spectra. A
characteristic internucleosomal ladder of DNA fragmentation was
also observed after 30 min of treatment. Moreover, the pH values
were significantly increases upon treatment due to the presence of Cs
and Rb nanoparticles in the PMF fraction. These results support the
previous findings that PMF is selective anticancer agent and can
produce apoptosis to A549 cells.
Abstract: This paper presents the experimental results on
artificial ageing test of 22 kV XLPE cable for distribution system
application in Thailand. XLPE insulating material of 22 kV cable
was sliced to 60-70 μm in thick and was subjected to ac high voltage
at 23
Ôùª
C, 60
Ôùª
C and 75
Ôùª
C. Testing voltage was constantly applied to
the specimen until breakdown. Breakdown voltage and time to
breakdown were used to evaluate life time of insulating material.
Furthermore, the physical model by J. P. Crine for predicts life time
of XLPE insulating material was adopted as life time model and was
calculated in order to compare the experimental results. Acceptable
life time results were obtained from Crine-s model comparing with
the experimental result. In addition, fourier transform infrared
spectroscopy (FTIR) for chemical analysis and scanning electron
microscope (SEM) for physical analysis were conducted on tested
specimens.
Abstract: The spiral angle of the elementary cellulose fibril in
the wood cell wall, often called microfibril angle, (MFA). Microfibril
angle in hardwood is one of the key determinants of solid timber
performance due to its strong influence on the stiffness, strength,
shrinkage, swelling, thermal-dynamics mechanical properties and
dimensional stability of wood. Variation of MFA (degree) in the S2
layer of the cell walls among Acacia mangium trees was determined
using small-angle X-ray scattering (SAXS). The length and
orientation of the microfibrils of the cell walls in the irradiated
volume of the thin samples are measured using SAXS and optical
microscope for 3D surface measurement. The undetermined
parameters in the analysis are the MFA, (M) and the standard
deviation (σФ) of the intensity distribution arising from the wandering
of the fibril orientation about the mean value. Nine separate pairs of
values are determined for nine different values of the angle of the
incidence of the X-ray beam relative to the normal to the radial
direction in the sample. The results show good agreement. The
curve distribution of scattered intensity for the real cell wall structure
is compared with that calculated with that assembly of rectangular
cells with the same ratio of transverse to radial cell wall length. It is
demonstrated that for β = 45°, the peaks in the curve intensity
distribution for the real and the rectangular cells coincide. If this
peak position is Ф45, then the MFA can be determined from the
relation M = tan-1 (tan Ф45 / cos 45°), which is precise for rectangular
cells. It was found that 92.93% of the variation of MFA can be
attributed to the distance from pith to bark. Here we shall present our
results of the MFA in the cell wall with respect to its shape, structure
and the distance from pith to park as an important fast check and yet
accurate towards the quality of wood, its uses and application.
Abstract: Bone remodeling occurs by the balanced action of
bone resorbing osteoclasts (OC) and bone-building osteoblasts.
Increased bone resorption by excessive OC activity contributes
to malignant and non-malignant diseases including osteoporosis.
To study OC differentiation and function, OC formed in
in vitro cultures are currently counted manually, a tedious
procedure which is prone to inter-observer differences. Aiming
for an automated OC-quantification system, classification of
OC and precursor cells was done on fluorescence microscope
images based on the distinct appearance of fluorescent nuclei.
Following ellipse fitting to nuclei, a combination of eight
features enabled clustering of OC and precursor cell nuclei.
After evaluating different machine-learning techniques, LOGREG
achieved 74% correctly classified OC and precursor cell
nuclei, outperforming human experts (best expert: 55%). In
combination with the automated detection of total cell areas,
this system allows to measure various cell parameters and most
importantly to quantify proteins involved in osteoclastogenesis.
Abstract: The effect of antifungal compound from Bacillus
subtilis strain LB5 was tested against conidial germination of
Colletotrichum gloeosporioides and Pestalotiopsis eugeniae, causal
agent of anthracnose and fruit rot of wax apple, respectively.
Observation under scanning electron microscope and light compound
microscope revealed that conidial germination was completely
inhibited when treated with culture broth, culture filtrate, or crude
extract from strain LB5. Identification of purified antifungal
compound produced by strain LB5 in cell-free supernatant by nuclear
magnetic resonance and fast atom bombardment showed that the
active compound was iturin A-2.