Abstract: Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.
Abstract: In recent years, several severe large-scale influenza
outbreaks happened in many countries, such as SARS in 2005 or
H1N1 in 2009. Those influenza Epidemics have greatly impacts not
only on people-s life and health, but medical systems in different
countries. Although severe diseases are more experienced, they are not
fully controlled. Governments have different policies to control the
spreads of diseases. However, those policies have both positive and
negative social or economical influence on people and society.
Therefore, it is necessary and essential to develop an appropriate
model for evaluations of policies. Consequently, a proper measure can
be implemented to confront the diseases. The main goal of this study is
to develop a SIR-based model for the further evaluations of the
candidate policies during the influenza outbreaks.
Abstract: This study investigated climatic factors associated
with influenza cases in Southern Thailand. The main aim for use
regression analysis to investigate possible causual relationship of
climatic factors and variability between the border of the Andaman
Sea and the Gulf of Thailand. Southern Thailand had the highest
Influenza incidences among four regions (i.e. north, northeast, central
and southern Thailand). In this study, there were 14 climatic factors:
mean relative humidity, maximum relative humidity, minimum
relative humidity, rainfall, rainy days, daily maximum rainfall,
pressure, maximum wind speed, mean wind speed, sunshine duration,
mean temperature, maximum temperature, minimum temperature,
and temperature difference (i.e. maximum – minimum temperature).
Multiple stepwise regression technique was used to fit the statistical
model. The results indicated that the mean wind speed and the
minimum relative humidity were positively associated with the
number of influenza cases on the Andaman Sea side. The maximum
wind speed was positively associated with the number of influenza
cases on the Gulf of Thailand side.
Abstract: Nigella sativa L. is an aromatic plant belonging to the
family Ranunculaceae. It has been used traditionally, especially in the
middle East and India, for the treatment of asthma, cough, bronchitis,
headache, rheumatism, fever, influenza and eczema. Several
biological activities have been reported in Nigella sativa seeds,
including antioxidant. In this context we tried to estimate the
antioxidant activity of various extracts prepared from Nigella sativa
seeds, methanolic extract (ME), chloroformic extract (CE), hexanic
extract (HE : fixed oil), ethyl acetate extract (EAE) water extract
(WE). The Folin-Ciocalteu assay showed that CE and EAE contained
high level of phenolic compounds 81.31 and 72.43μg GAE/mg of
extract respectively. Similarly, the CE and EAE exhibited the highest
DPPH radical scavenging activity, with IC50 values of 106.56μg/ml
and 121.62μg/ml respectively. In addition, CE and HE showed the
most scavenging activity against superoxide radical generated in the
PMS-NADH-NBT system with respective IC50 values of 361.86
μg/ml and 371.80 μg/ml, which is comparable to the activity of the
standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating
capacity assay showed that WE, EAE and ME are the most active
with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition
of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene
bleaching assay, this showed a highest relative antioxidant
activity with CE and EAE (69.82% of inhibition). The antioxidant
activities of the methanolic extract and the fixed oil are confirmed by
an in vivo assay in mice, the daily oral administration of methanolic
extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day)
during 21 days, resulted in a significant enhancement of the blood
total antioxidant capacity (measured by KRL test) and the plasmatic
antioxidant capacity towards DPPH radical.
Abstract: A new strain of Type A influenza virus can cause the
transmission of H1N1 virus. This virus can spread between the
people by coughing and sneezing. Because the people are always
movement, so this virus can be easily spread. In this study, we
construct the dynamical network model of H1N1 virus by separating
the human into five groups; susceptible, exposed, infectious,
quarantine and recovered groups. The movement of people between
houses (local level) is considered. The behaviors of solutions to our
dynamical model are shown for the different parameters.