Abstract: The disposal and the treatment of sewage sludge is an
expensive and environmentally complex problem. In this work, a
lipopeptide biosurfactant extracted from corn steep liquor was used
as ecofriendly and cost-competitive alternative for the mobilization
and bioremediation of fluorene in sewage sludge. Results have
demonstrated that this biosurfactant has the capability to mobilize
fluorene to the aqueous phase, reducing the amount of fluorene in the
sewage sludge from 484.4 mg/Kg up to 413.7 mg/Kg and 196.0
mg/Kg after 1 and 27 days respectively. Furthermore, once the
fluorene was extracted the lipopeptide biosurfactant contained in the
aqueous phase allowed the biodegradation, up to 40.5% of the initial
concentration of this polycyclic aromatic hydrocarbon.
Abstract: Microbial production of antimicrobials as biopreservatives is the major area of focus nowadays due to increased interest of consumers towards natural and safe preservation of ready to eat food products. The agro-industrial byproduct based medium and optimized process conditions can contribute in economical production of bacteriocins. Keeping this in view, the present investigation was carried out on agro-industrial byproducts utilization for the production of bacteriocin using Enterococcus faecium BS13 isolated from local fermented food. Different agro-industrial byproduct based carbon sources (whey, potato starch liquor, kinnow peel, deoiledrice bran and molasses), nitrogen sources (soya okra, pea pod and corn steep liquor), metal ions and surfactants were tested for optimal bacteriocin production. The effect of various process parameters such as pH, temperature, inoculum level, agitation and time were also tested on bacteriocin production. The optimized medium containing whey, supplemented with 4%corn steep liquor and polysorbate-80 displayed maximum bacteriocin activity with 2% inoculum, at pH 6.5, temperature 40oC under shaking conditions (100 rpm).