Bioremediation of Sewage Sludge Contaminated with Fluorene Using a Lipopeptide Biosurfactant
The disposal and the treatment of sewage sludge is an
expensive and environmentally complex problem. In this work, a
lipopeptide biosurfactant extracted from corn steep liquor was used
as ecofriendly and cost-competitive alternative for the mobilization
and bioremediation of fluorene in sewage sludge. Results have
demonstrated that this biosurfactant has the capability to mobilize
fluorene to the aqueous phase, reducing the amount of fluorene in the
sewage sludge from 484.4 mg/Kg up to 413.7 mg/Kg and 196.0
mg/Kg after 1 and 27 days respectively. Furthermore, once the
fluorene was extracted the lipopeptide biosurfactant contained in the
aqueous phase allowed the biodegradation, up to 40.5% of the initial
concentration of this polycyclic aromatic hydrocarbon.
[1] S.K. Samanta, O.V. Singh and R.K. Jain, “Polycyclic aromatic
hydrocarbons: environmental pollution and bioremediation”, Trends
Biotechnol. vol. 20, 2002, pp. 243–248.
[2] C.A. Menzie, B.B. Potocki and J. Santodonato, “Exposure to
carcinogenic PAHs in the environment”, Environ. Sci. Technol. vol. 26,
1992, pp. 1278–1284.
[3] United States Environmental Protection Agency (USEPA), Appendix A
to 4 CFR Part 423, November (2010). Available from:
http://www.epa.gov/waterscience/methods/pollutants.htm
[4] J. Hall, “Ecological and economical balance for sludge management
options”, WRcplc, Medmenham, Marlow, SL7 2HD, United Kingdom.
[5] M.T. Pena, M.C. Casais, M.C. Mejuto and R. Cela, “Development of a
sample preparation procedure of sewage sludge samples for the
determination of polycyclic aromatic hydrocarbons based on selective
pressurized liquid extraction”, J. Chromatogr. A vol. 1217, 2010, pp.
425–435.
[6] Council of the European Community, 27 April, 2000.Working document
on Sludge, 3rd Draft, Brussels.
[7] D.T. Sponza and O. Gok, “Aerobic biodegradation and inhibition
kinetics of poly-aromatic hydrocarbons (PAHs) in a petrochemical
industry wastewater in the presence of biosurfactants”, J. Chem.
Technol. Biotechnol. vol. 87, 2012, pp. 658–672.
[8] E.C. Souza, T.C. Vessoni-Penn and R.P. De Souza Oliveira,
“Biosurfactant-enhanced hydrocarbon bioremediation: An overview”,
Int. Biodeter. Biodegr. vol. 89, 2014, pp. 88–94.
[9] A.B. Moldes, R. Paradelo, X. Vecino, J.M. Cruz, E. Gudiña, L.
Rodrigues, J.A. Teixeira, J.M. Domínguez and M.T. Barral, “Partial
characterization of biosurfactant from Lactobacillus pentosus and
comparison with sodium dodecyl sulphate for the bioremediation of
hydrocarbon contaminated soil”, BioMed Res. Int.2013, Article number
961842.
[10] A.B. Moldes, R. Paradelo, D. Rubinos, R. Devesa-Rey, J.M. Cruz and
M.T. Barral, “Ex situ treatment of hydrocarbon-contaminated soil using
biosurfactants from Lactobacillus pentosus”, J. Agr. Food Chem. vol.
59, 2011, pp. 9443–9447.
[11] M. Pacwa-Płociniczak, G.A. Płaza, Z. Piotrowska-Seget and S.S.
Cameotra, “Environmental Applications of Biosurfactants: Recent
Advances”, Int. J. Mol. Sci.vol. 12, 2011, pp. 633–654.
[12] S.S. Cameotra and R.S. Makkar, “Biosurfactant-enhanced
bioremediation of hydrophobic pollutants”, Pure Appl. Chem.vol. 82,
2010, pp. 97–116.
[13] A.B. Moldes, A.M. Torrado, M.T. Barral and J.M. Domínguez,
“Evaluation of biosurfactant production from various agricultural
residues by Lactobacillus pentosus”, J. Agrc. Food Chem. vol. 55,2007,
pp. 4481−4486.
[14] X. Vecino, L. Barbosa-Pereira, R. Devesa-Rey, J.M. Cruz and A.B.
Moldes, “Study of the surfactant properties of aqueous stream from the
corn milling industry”, J. Agr. Food Chem. vol. 62, 2014, pp. 5451–
5457.
[15] H.R. Rogers, “Sources, behaviour and fate of organic contaminants
during sewage treatment and in sewage sludges”, Sci. Total
Environ.vol.185, 1996, pp. 3–26. [16] W. Zhou and L. Zhu, “Enhanced desorption of phenanthrene from
contaminated soil using anionic/nonionic mixed surfactant”, Environ.
Pollut. vol. 147, 2007, pp. 350–357
[17] C. N. Mulligan, “Environmental applications for biosurfactants”,
Environ. Pollut. vol. 133, 2005, pp. 183–198.
[18] J. Ma, L. Xu, L. Jia, “Degradation of polycyclic aromatic hydrocarbons
by Pseudomonas sp. JM2 isolated from active sewage sludge of
chemical plant”, J. Environ. Sci. vol. 24, 2012, pp. 2141–2148.
[19] M.P. Kolomytseva, D. Randazzo, B.P. Baskunov, A. Scozzafava, F.
Briganti, L.A. Golovleva, “Role of surfactants in optimizing fluorene
assimilation and intermediate formation by Rhodococcus rhodochrous
VKM B-246”, BioresourceTechnol.vol. 100, 2009, pp. 839–844.
[1] S.K. Samanta, O.V. Singh and R.K. Jain, “Polycyclic aromatic
hydrocarbons: environmental pollution and bioremediation”, Trends
Biotechnol. vol. 20, 2002, pp. 243–248.
[2] C.A. Menzie, B.B. Potocki and J. Santodonato, “Exposure to
carcinogenic PAHs in the environment”, Environ. Sci. Technol. vol. 26,
1992, pp. 1278–1284.
[3] United States Environmental Protection Agency (USEPA), Appendix A
to 4 CFR Part 423, November (2010). Available from:
http://www.epa.gov/waterscience/methods/pollutants.htm
[4] J. Hall, “Ecological and economical balance for sludge management
options”, WRcplc, Medmenham, Marlow, SL7 2HD, United Kingdom.
[5] M.T. Pena, M.C. Casais, M.C. Mejuto and R. Cela, “Development of a
sample preparation procedure of sewage sludge samples for the
determination of polycyclic aromatic hydrocarbons based on selective
pressurized liquid extraction”, J. Chromatogr. A vol. 1217, 2010, pp.
425–435.
[6] Council of the European Community, 27 April, 2000.Working document
on Sludge, 3rd Draft, Brussels.
[7] D.T. Sponza and O. Gok, “Aerobic biodegradation and inhibition
kinetics of poly-aromatic hydrocarbons (PAHs) in a petrochemical
industry wastewater in the presence of biosurfactants”, J. Chem.
Technol. Biotechnol. vol. 87, 2012, pp. 658–672.
[8] E.C. Souza, T.C. Vessoni-Penn and R.P. De Souza Oliveira,
“Biosurfactant-enhanced hydrocarbon bioremediation: An overview”,
Int. Biodeter. Biodegr. vol. 89, 2014, pp. 88–94.
[9] A.B. Moldes, R. Paradelo, X. Vecino, J.M. Cruz, E. Gudiña, L.
Rodrigues, J.A. Teixeira, J.M. Domínguez and M.T. Barral, “Partial
characterization of biosurfactant from Lactobacillus pentosus and
comparison with sodium dodecyl sulphate for the bioremediation of
hydrocarbon contaminated soil”, BioMed Res. Int.2013, Article number
961842.
[10] A.B. Moldes, R. Paradelo, D. Rubinos, R. Devesa-Rey, J.M. Cruz and
M.T. Barral, “Ex situ treatment of hydrocarbon-contaminated soil using
biosurfactants from Lactobacillus pentosus”, J. Agr. Food Chem. vol.
59, 2011, pp. 9443–9447.
[11] M. Pacwa-Płociniczak, G.A. Płaza, Z. Piotrowska-Seget and S.S.
Cameotra, “Environmental Applications of Biosurfactants: Recent
Advances”, Int. J. Mol. Sci.vol. 12, 2011, pp. 633–654.
[12] S.S. Cameotra and R.S. Makkar, “Biosurfactant-enhanced
bioremediation of hydrophobic pollutants”, Pure Appl. Chem.vol. 82,
2010, pp. 97–116.
[13] A.B. Moldes, A.M. Torrado, M.T. Barral and J.M. Domínguez,
“Evaluation of biosurfactant production from various agricultural
residues by Lactobacillus pentosus”, J. Agrc. Food Chem. vol. 55,2007,
pp. 4481−4486.
[14] X. Vecino, L. Barbosa-Pereira, R. Devesa-Rey, J.M. Cruz and A.B.
Moldes, “Study of the surfactant properties of aqueous stream from the
corn milling industry”, J. Agr. Food Chem. vol. 62, 2014, pp. 5451–
5457.
[15] H.R. Rogers, “Sources, behaviour and fate of organic contaminants
during sewage treatment and in sewage sludges”, Sci. Total
Environ.vol.185, 1996, pp. 3–26. [16] W. Zhou and L. Zhu, “Enhanced desorption of phenanthrene from
contaminated soil using anionic/nonionic mixed surfactant”, Environ.
Pollut. vol. 147, 2007, pp. 350–357
[17] C. N. Mulligan, “Environmental applications for biosurfactants”,
Environ. Pollut. vol. 133, 2005, pp. 183–198.
[18] J. Ma, L. Xu, L. Jia, “Degradation of polycyclic aromatic hydrocarbons
by Pseudomonas sp. JM2 isolated from active sewage sludge of
chemical plant”, J. Environ. Sci. vol. 24, 2012, pp. 2141–2148.
[19] M.P. Kolomytseva, D. Randazzo, B.P. Baskunov, A. Scozzafava, F.
Briganti, L.A. Golovleva, “Role of surfactants in optimizing fluorene
assimilation and intermediate formation by Rhodococcus rhodochrous
VKM B-246”, BioresourceTechnol.vol. 100, 2009, pp. 839–844.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:70757", author = "X. Vecino and J. M. Cruz and A. Moldes", title = "Bioremediation of Sewage Sludge Contaminated with Fluorene Using a Lipopeptide Biosurfactant", abstract = "The disposal and the treatment of sewage sludge is an
expensive and environmentally complex problem. In this work, a
lipopeptide biosurfactant extracted from corn steep liquor was used
as ecofriendly and cost-competitive alternative for the mobilization
and bioremediation of fluorene in sewage sludge. Results have
demonstrated that this biosurfactant has the capability to mobilize
fluorene to the aqueous phase, reducing the amount of fluorene in the
sewage sludge from 484.4 mg/Kg up to 413.7 mg/Kg and 196.0
mg/Kg after 1 and 27 days respectively. Furthermore, once the
fluorene was extracted the lipopeptide biosurfactant contained in the
aqueous phase allowed the biodegradation, up to 40.5% of the initial
concentration of this polycyclic aromatic hydrocarbon.", keywords = "Fluorene, lipopeptide biosurfactant, mobilization,
sewage sludge.", volume = "9", number = "6", pages = "751-4", }
