Abstract: Cereals are considered as a strategic product in human life and their demand is increasing with the growth of world population. Increasing wheat production is important for the country. One of the ways to solve the problem is to develop and implement new, environmentally and economically acceptable technologies. Such technologies include pre-sowing treatment of seed with a laser and associative nitrogen-fixing bacteria - Azospirillum brasilense. In the region there are the wheat varieties - Dika and Lomtagora, which are among the most common in Georgia. Dika is a frost-resistant wheat, with a high ability to adapt to the environment, resistant to falling and it is sown in highlands. Lomtagora 126 differs with its winter and drought resistance, and it has a great ability to germinate. Lomtagora is characterized by a strong root system and a high budding capacity. It is an early variety, fall-resistant, easy to thresh and suitable for mechanized harvesting with large and red grains. This paper presents some preliminary experimental results where a continuous CO2 laser with a power of 25-40 W was used to radiate grains at a flow rate of 10 and 15 cm/sec. The treatment was carried out on grains of the Triticum aestivum L. var. Lutescens (local variety name - Lomtagora 126), and Triticum carthlicum Nevski (local variety name - Dika). Here the grains were treated with A. brasilense isolate (108-109 CFU/ml), which was isolated from the rhizosphere of wheat. It was observed that the germination of the wheat was not significantly influenced by either laser or bacteria treatment. The results of our research show that combined treatment with laser and A. brasilense significantly influenced the germination of wheat. In the case of the Lomtagora 126 variety, grains were exposed to the beam on a speed of 10 cm/sec, only slightly improved the growth for 38-day seedlings, in case of exposition of grains with a speed of 15 cm/sec - by 23%. Treatment of seeds with A. brasilense in both exposed and non-exposed variants led to an improvement in the growth of seedlings, with A. brasilense alone - by 22%, and with combined treatment of grains - by 29%. In the case of the Dika variety, only exposure led to growth by 8-9%, and the combined treatment - by 10-15%, in comparison with the control variant. Superior effect on growth of seedlings of different varieties was achieved with the combinations of laser treatment on grains in a beam of 15 cm/sec (radiation power 30-40 W) and in addition of A. brasilense - nitrogen fixing bacteria. Therefore, this is a promising application of A. brasilense as active agents of bacterial fertilizers due to their ability of molecular nitrogen fixation in cereals in combination with laser irradiation: choosing a proper strain gives a good ability to colonize roots of agricultural crops, providing a high nitrogen-fixing ability and the ability to mobilize soil phosphorus, and laser treatment stimulates natural processes occurring in plant cells, will increase the yield.
Abstract: The main objective of this study was to determine the
effects of Nitrogen fixing bacteria and manure application on the seed
yield and yield components in black cumin (Nigella sativa L.). The
experiment was carried out at the RAN Research Station in
Firouzkouh in 2012. A 4×4 factorial experiment, arranged in a
randomized complete blocks designed with three replications.
Nitrogen fixing bacteria at 4 levels (control, Azotobacter,
Azospirillum and Azotobacter + Azospirillum) and manure
application at 4 levels (0, 2.5, 5 and 7.5 ton ha-1) were used at this
investigation. The present results have shown that the highest height,
1000 seeds weight, seed number per follicle, follicle yield, seed yield
and harvest index were obtained after using Azotobacter and
Azospirillum, simultaneously. Manure application only effects on
follicle yield and by 5ton manure ha-1 the highest follicle yield
obtained. Results of this investigation showed that the maximum seed
yield obtained when Aotobacter+Azospirillum inoculated with black
cumin seeds and 5 ton manure ha-1 applied. According to the results
of this investigation the integrated management of Azotobacter and
Azospirillum with manure application is the best treatment for
achieving the maximum quantitative charactersitics of Black cumin.
Abstract: In order to investigate the effect of Plant Growth
Promoting Rhizobacteria (PGPR) and rhizobium bacteria on grain
yield and some agronomic traits of mungbean (Vigna radiate L.), an
experiment was carried out based on randomized complete block
design with three replications in Malekshahi, Ilam province, Iran
during 2012-2013 cropping season. Experimental treatments
consisted of control treatment, inoculation with rhizobium bacteria,
rhizobium bacteria and Azotobacter, rhizobium bacteria and
Azospirillum, rhizobium bacteria and Pseudomonas, rhizobium
bacteria, Azotobacter and Azospirillum, rhizobium bacteria,
Azotobacter and Pseudomonas, rhizobium bacteria, Azospirillum and
Pseudomonas and rhizobium bacteria, Azotobacter, Azospirillum and
Pseudomonas. The results showed that the effect of PGPR and
rhizobium bacteria were significant affect on grain and its
components in mungbean plant. Grain yield significantly increased
by PGPR and rhizobium bacteria, so that the maximum grain yield
was obtained from rhizobium bacteria + Azospirillum +
Pseudomonas with the amount of 2287 kg.ha-1 as compared to
control treatment. Excessive application of chemical fertilizers causes
environmental and economic problems. That is, the overfertilization
of P and N leads to pollution due to soil erosion and runoff water, so
the use of PGPR and rhizobium bacteria can be justified due to
reduce input costs, increase in grain yield and environmental friendly.
Abstract: The effect of plant growth-promoting rhizobacteria
(PGPR) on seed germination, seedling growth and yield of field
grown maize were evaluated in three experiments. In these
experiments six bacterial strains include P.putida strain R-168,
P.fluorescens strain R-93, P.fluorescens DSM 50090, P.putida
DSM291, A.lipoferum DSM 1691, A.brasilense DSM 1690 were
used. Results of first study showed seed Inoculation significantly
enhanced seed germination and seedling vigour of maize. In second
experiment, leaf and shoot dry weight and also leaf surface area
significantly were increased by bacterial inoculation in both sterile
and non-sterile soil. The results showed that inoculation with
bacterial treatments had a more stimulating effect on growth and
development of plants in nonsterile than sterile soil. In the third
experiment, Inoculation of maize seeds with all bacterial strains
significantly increased plant height, 100 seed weight, number of seed
per ear and leaf area .The results also showed significant increase in
ear and shoot dry weight of maize.
Abstract: The problem of agricultural-soil pollution is closely
linked to the production of ecologically pure foodstuffs and to human health. An important task, therefore, is to rehabilitate agricultural
soils with the help of state-of-the-art biotechnologies, based on the use of metal-accumulating plants. In this work, on the basis of
literature data and the results of prior research from this laboratory, plants were selected for which the growing technology is well
developed and which are widespread locally: sugar sorghum (Sorghum saccharatum), sudangrass (Sorghum sudanense (Piper.)
Stapf.), and sunflower (Helianthus annuus L.). I report on laboratory
experiments designed to study the influence of synthetic indole-3-
acetic acid and the extracellular indole-3-acetic acid released by the
plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245 on growth of and arsenic accumulation by these plants.