Abstract: Twenty four New Zealand white rabbits (12 does and
12 bucks) and twenty four Flanders (12 does and 12 bucks) rabbits,
allotted into two feeding regime (6 for each breed, 3 males and 3
females) first one fed commercial ration and second one fed
commercial diet plus sodium butyrate (300 g/ton). The obtained
results showed that at end of 8th week experimental period New
Zealand white rabbits were heavier body weight than Flanders rabbits
(1934.55+39.05 vs. 1802.5+30.99 g); significantly high body weight
gain during experimental period especially during 8th week
(136.1+3.5 vs. 126.8+1.8 g/week); better feed conversion ratio during
all weeks of experiment from first week (3.07+0.16 vs. 3.12+0.10)
till the 8th week of experiment (5.54+0.16 vs. 5.76+0.07) with
significantly high dressing percentages (0.54+0.01 vs. 0.52+0.01).
Also all carcass cuts were significantly high in New Zealand white
rabbits than Flanders. Females rabbits (at the same age) were lower
body weight than males from start of experiment (941.1+39.8
vs.972.1+33.5 g) till the end of experiment (1833.64+37.69 vs.
1903.41+36.93 g); gained less during all weeks of experiment except
during 8th week (132.1+2.3 vs. 130.9+3.4 g/week), with lower
dressing percentage (0.52+0.01 vs. 0.53+0.01) and lighter carcass
cuts than males, however, they had better feed conversion ratio
during 1st week, 7th week and 8th week of experiment. Addition of
300g sodium butyrate/ton of rabbit increased the body weight of
rabbits at the end of experimental period (1882.71+26.45 vs.
1851.5+49.82 g); improve body weight gain at 3rd, 4th, 5th, 6th and
7th week of experiment and significantly improve feed conversion
ratio during all weeks of the experiment from 1st week (2.85+0.07
vs. 3.30+0.15) till the 8th week of the experiment (5.51+0.12 vs.
5.77+0.12). Also the dressing percentage was higher in Sodium
butyrate fed groups than control one (0.53+0.01 vs. 0.52+0.01) and
the most important results of feeding sodium butyrate is the reducing
of the mortality percentage in rabbits during 8 week experiment to
zero percentage as compared with 16% in control group.
Abstract: Steroidal hormones with the efficient changes on the epiphyseal growth plate may influence tissue structure properties. Presents paper to investigate the effects of gonadectomy in the pattern distribution of the epiphyseal structure. Fifteen adult female New Zealand white rabbits were separated into three groups. One group was intact and others groups were selected for surgical operation. From these two groups, one group carried out steroidal administration. The results obtained showed that there is no statistically difference in the mean diameter of the growth plate cells between all three groups. The maximum value of the cartilage cells were allocated to the gonadectomized group and the minimum number were observed in Hormonal induced group significantly. Growth plate height was significantly greater in gonadectomized group than in two other groups.
Abstract: The present study was designed to investigate the
cardio protective role of chronic oral administration of alcoholic
extract of Terminalia arjuna in in-vivo ischemic reperfusion injury
and the induction of HSP72. Rabbits, divided into three groups, and
were administered with the alcoholic extract of the bark powder of
Terminalia arjuna (TAAE) by oral gavage [6.75mg/kg: (T1) and
9.75mg/kg: (T2), 6 days /week for 12 weeks]. In open-chest
Ketamine pentobarbitone anaesthetized rabbits, the left anterior
descending coronary artery was occluded for 15 min of ischemia
followed by 60 min of reperfusion. In the vehicle-treated group,
ischemic-reperfusion injury (IRI) was evidenced by depression of
global hemodynamic function (MAP, HR, LVEDP, peak LV (+) & (-
) (dP/dt) along with depletion of HEP compounds. Oxidative stress
in IRI was evidenced by, raised levels of myocardial TBARS and
depletion of endogenous myocardial antioxidants GSH, SOD and
catalase. Western blot analysis showed a single band corresponding
to 72 kDa in homogenates of hearts from rabbits treated with both the
doses. In the alcoholic extract of the bark powder of Terminalia
arjuna treatment groups, both the doses had better recovery of
myocardial hemodynamic function, with significant reduction in
TBARS, and rise in SOD, GSH, catalase were observed. The results
of the present study suggest that the alcoholic extract of the bark
powder of Terminalia arjuna in rabbit induces myocardial HSP 72
and augments myocardial endogenous antioxidants, without causing
any cellular injury and offered better cardioprotection against
oxidative stress associated with myocardial IR injury.
Abstract: A series of Ti based shape memory alloys with
composition of Ti50Ni49Cr1, Ti50Ni47Cr3 and Ti50Ni45Cr5 were
developed by vacuum arc-melting under a purified argon atmosphere.
The histometric and corrosion evaluation of Ti-Ni-Cr shape memory
alloys have been considered in this research work. The alloys were
developed by vacuum arc melting and implanted subcutaneously in
rabbits for 4, 8 and 12 weeks. Metallic implants were embedded in
order to determine the outcome of implantation on histometric and
corrosion evaluation of Ti-Ni-Cr metallic strips. Encapsulating
membrane formation around the alloys was minimal in the case of all
materials. After histomorphometric analyses it was possible to
demonstrate that there were no statistically significant differences
between the materials. Corrosion rate was also determined in this
study which is within acceptable range. The results showed the Ti-
Ni-Cr alloy was neither cytotoxic, nor have any systemic reaction on
living system in any of the test performed. Implantation shows good
compatibility and a potential of being used directly in vivo system.
Abstract: Snake bite cases in Malaysia most often involve the
species Naja-naja and Calloselasma rhodostoma. In keeping with the
need for a rapid snake venom detection kit in a clinical setting, plate
and dot-ELISA test for the venoms of Naja-naja sumatrana,
Calloselasma rhodostoma and the cobra venom fraction V antigen
was developed. Polyclonal antibodies were raised and further used to
prepare the reagents for the dot-ELISA test kit which was tested in
mice, rabbit and virtual human models. The newly developed dot-
ELISA kit was able to detect a minimum venom concentration of
244ng/ml with cross reactivity of one antibody type. The dot-ELISA
system was sensitive and specific for all three snake venom types in
all tested animal models. The lowest minimum venom concentration
detectable was in the rabbit model, 244ng/ml of the cobra venom
fraction V antigen. The highest minimum venom concentration was
in mice, 1953ng/ml against a multitude of venoms. The developed
dot-ELISA system for the detection of three snake venom types was
successful with a sensitivity of 95.8% and specificity of 97.9%.
Abstract: We aimed to investigate how can target and optimize
pulmonary delivery distribution by changing physicochemical
characteristics of instilled liquid.Therefore, we created a new liquids
group:
a. eligible for desired distribution within lung because of
assorted physicochemical characteristics
b. capable of being augmented with a broad range of
chemicals inertly
c. no interference on respiratory function
d. compatible with airway surface liquid
We developed forty types of new liquid,were composed of
Carboxymethylcellulose sodium,Glycerin and different types of
Polysorbates.Viscosity was measured using a Programmable
Rheometer and surface tension by KRUSS Tensiometer.We
subsequently examined the liquids and delivery protocols by simple
and branched glass capillary tube models of airways.Eventually,we
explored pulmonary distribution of liquids being augmented with
technetium-99m in mechanically ventilated rabbits.We used a single
head large field of view gamma camera.Kinematic viscosity between
0.265Stokes and 0.289Stokes,density between 1g/cm3 and 1.5g/cm3
and surface tension between 25dyn/cm and 35dyn/cm were the most
acceptable.
Abstract: The present study has been taken to explore the
screening of in vitro antimicrobial activities of D-galactose-binding
sponge lectin (HOL-30). HOL-30 was purified from the marine
demosponge Halichondria okadai by affinity chromatography. The
molecular mass of the lectin was determined to be 30 kDa with a
single polypeptide by SDS-PAGE under non-reducing and reducing
conditions. HOL-30 agglutinated trypsinized and glutaraldehydefixed
rabbit and human erythrocytes with preference for type O
erythrocytes. The lectin was subjected to evaluation for inhibition of
microbial growth by the disc diffusion method against eleven human
pathogenic gram-positive and gram-negative bacteria. The lectin
exhibited strong antibacterial activity against gram-positive bacteria,
such as Bacillus megaterium and Bacillus subtilis. However, it did
not affect against gram-negative bacteria such as Salmonella typhi
and Escherichia coli. The largest zone of inhibition was recorded of
Bacillus megaterium (12 in diameter) and Bacillus subtilis (10 mm in
diameter) at a concentration of the lectin (250 μg/disc). On the other
hand, the antifungal activity of the lectin was investigated against six
phytopathogenic fungi based on food poisoning technique. The lectin
has shown maximum inhibition (22.83%) of mycelial growth of
Botrydiplodia theobromae at a concentration of 100 μg/mL media.
These findings indicate that the lectin may be of importance to
clinical microbiology and have therapeutic applications.
Abstract: Attachment of the circulating monocytes to the
endothelium is the earliest detectable events during formation of
atherosclerosis. The adhesion molecules, chemokines and matrix
proteases genes were identified to be expressed in atherogenesis.
Expressions of these genes may influence structural integrity of the
luminal endothelium. The aim of this study is to relate changes in the
ultrastructural morphology of the aortic luminal surface and gene
expressions of the endothelial surface, chemokine and MMP-12 in
normal and hypercholesterolemic rabbits. Luminal endothelial
surface from rabbit aortic tissue was examined by scanning electron
microscopy (SEM) using low vacuum mode to ascertain
ultrastructural changes in development of atherosclerotic lesion. Gene
expression of adhesion molecules, MCP-1 and MMP-12 were studied
by Real-time PCR. Ultrastructural observations of the aortic luminal
surface exhibited changes from normal regular smooth intact
endothelium to irregular luminal surface including marked globular
appearance and ruptures of the membrane layer. Real-time PCR
demonstrated differentially expressed of studied genes in
atherosclerotic tissues. The appearance of ultrastructural changes in
aortic tissue of hypercholesterolemic rabbits is suggested to have
relation with underlying changes of endothelial surface molecules,
chemokine and MMP-12 gene expressions.
Abstract: The aim of the present investigation was to compare
sex differences in thyroid gland structure of rabbits. Five adult male
and five adult female (3.1-3.5 kg body weight) New Zealand white
rabbits were used in the experiment. Results showed that at light
microscopic level, there was no sex difference in microscopic
appearance of the thyroid glands. At electron microscopic level,
however, the mitochondria and the microvilli of the follicular cells
are more numerous and the Golgi complex is also more extensive in
male rabbits in comparison to females. Results obtained from
micrometric measurements showed that the volume density of the
follicles is higher in males than in females, but the differences are not
statistically significant .The volume density of epithelium and the
height of follicular cells are significantly greater in males than in
females and reverse is true about the volume density of interstitium
(p
Abstract: Intravitreal injection (IVI) is the most common treatment for eye posterior segment diseases such as endopthalmitis, retinitis, age-related macular degeneration, diabetic retinopathy, uveitis, and retinal detachment. Most of the drugs used to treat vitreoretinal diseases, have a narrow concentration range in which they are effective, and may be toxic at higher concentrations. Therefore, it is critical to know the drug distribution within the eye following intravitreal injection. Having knowledge of drug distribution, ophthalmologists can decide on drug injection frequency while minimizing damage to tissues. The goal of this study was to develop a computer model to predict intraocular concentrations and pharmacokinetics of intravitreally injected drugs. A finite volume model was created to predict distribution of two drugs with different physiochemical properties in the rabbit eye. The model parameters were obtained from literature review. To validate this numeric model, the in vivo data of spatial concentration profile from the lens to the retina were compared with the numeric data. The difference was less than 5% between the numerical and experimental data. This validation provides strong support for the numerical methodology and associated assumptions of the current study.