Abstract: The objective of this work was to investigate flow
properties of powdered infant formula samples. Samples were
purchased at a local pharmacy and differed in composition. Lactose
free infant formula, gluten free infant formula and infant formulas
containing dietary fibers and probiotics were tested and compared
with a regular infant formula sample which did not contain any of
these supplements. Particle size and bulk density were determined
and their influence on flow properties was discussed. There were no
significant differences in bulk densities of the samples, therefore the
connection between flow properties and bulk density could not be
determined. Lactose free infant formula showed flow properties
different to standard supplement-free sample. Gluten free infant
formula with addition of probiotic microorganisms and dietary fiber
had the narrowest particle size distribution range and exhibited the
best flow properties. All the other samples exhibited the same
tendency of decreasing compaction coefficient with increasing flow
speed, which means they all become freer flowing with higher flow
speeds.
Abstract: Cow milk, is a product of the mammary gland and
soymilk is a beverage made from soybeans; it is the liquid that
remains after soybeans are soaked. In this research effort, we
compared nutritional parameters of this two kind milk such as total
fat, fiber, protein, minerals (Ca, Fe and P), fatty acids, carbohydrate,
lactose, water, total solids, ash, pH, acidity and calories content in
one cup (245 g). Results showed soymilk contains 4.67 grams of fat,
0.52 of fatty acids, 3.18 of fiber, 6.73 of protein, 4.43 of
carbohydrate, 0.00 of lactose, 228.51 of water, 10.40 of total solids
and 0.66 of ash, also 9.80 milligrams of Ca, 1.42 of Fe, and 120.05 of
P, 79 Kcal of calories, pH=6.74 and acidity was 0.24%. Cow milk
contains 8.15 grams of fat, 5.07 of fatty acids, 0.00 of fiber, 8.02 of
protein, 11.37 of carbohydrate, ´Çá4.27 of lactose, 214.69 of water,
12.90 of total solids, 1.75 of ash, 290.36 milligrams of Ca, 0.12 of
Fe, and 226.92 of P, 150 Kcal of calories, pH=6.90 and acidity was
0.21% . Soy milk is one of plant-based complete proteins and cow
milk is a rich source of nutrients as well. Cow milk is containing near
twice as much fat as and ten times more fatty acids do soymilk. Cow
milk contains greater amounts of mineral (except Fe) it contain more
than three hundred times the amount of Ca and nearly twice the
amount of P as does soymilk but soymilk contains more Fe (ten time
more) than does cow milk. Cow milk and soy milk contain nearly
identical amounts of protein and water and fiber is a big plus, dairy
has none. Although what we choose to drink is really a mater of
personal preference and our health objectives but looking at the
comparison, soy looks like healthier choices.
Abstract: The effects of irrigation with dairy factory wastewater
on soil properties were investigated at two sites that had received
irrigation for > 60 years. Two adjoining paired sites that had never
received DFE were also sampled as well as another seven fields from
a wider area around the factory. In comparison with paired sites that
had not received effluent, long-term wastewater irrigation resulted in
an increase in pH, EC, extractable P, exchangeable Na and K and
ESP. These changes were related to the use of phosphoric acid,
NaOH and KOH as cleaning agents in the factory. Soil organic C
content was unaffected by DFE irrigation but the size (microbial
biomass C and N) and activity (basal respiration) of the soil
microbial community were increased. These increases were
attributed to regular inputs of soluble C (e.g. lactose) present as milk
residues in the wastewater. Principal component analysis (PCA) of
the soils data from all 11sites confirmed that the main effects of DFE
irrigation were an increase in exchangeable Na, extractable P and
microbial biomass C, an accumulation of soluble salts and a liming
effect. PCA analysis of soil bacterial community structure, using
PCR-DGGE of 16S rDNA fragments, generally separated individual
sites from one another but did not group them according to irrigation
history. Thus, whilst the size and activity of the soil microbial
community were increased, the structure and diversity of the
bacterial community remained unaffected.
Abstract: It has been shown that pH 7,3 and 37 0C are the optimal condition for the growth of E. coli “ASAP". The cells grow well on Glucose, Lactose, D-Mannitol, D-Sorbitol, (+)-Xylose, L- (+)-Arabinose and Dulcitol. No growth has been observed on Sucrose, Inositol, Phenylalanine, and Tryptophan. The strain is sensitive to a range of antibiotics. The present study has demonstrated that E. coli “ASAP" inhibit the growth of S. enterica ATCC #700931 in vitro. The studies on conjugating activity has revealed no conjugant of E. coli “ASAP" with plasmid strains E. coli G35#59 and S. enterica ATCC #700931. On the other hand, the conjugants with low frequencies were obtained from E. coli “ASAP" with E. coli G35#61, and E. coli “ASAP" with randomly chosen isolate from healthy human gut microflora: E. coli E6. The results of present study have demonstrated improvements in gut microflora condition of patients with different diseases after the administration of “ASAP"
Abstract: A thirty Rahmani weaned male lambs of average body weight (27.28±1.40 kg) were randomly allotted to three similar groups, ten lambs in each, to study the benefit of commercial feed additives Tonilisat (Saccharomyces cerevisiae) and Roemin W2 (Lactobacillus acidophilus, Lactobacillus thermophilus, Bifidobacterium and Lactose) as growth promoters on lambs performance, digestibility, rumen activity and some blood constituents. The experiment lasted about 107 days. Three experimental groups were allotted as control group: received the basal ration, T1 group: received the basal ration supplemented with Tonilisat as (0.5kg/ ton concentrate feed mixture) and T2 group: received the basal ration supplemented with Roemin W2 (1kg/ ton concentrate feed mixture). Our study revealed that addition of Tonilisat significantly increased digestion coefficient of crude protein than that of the control group, Furthermore, the supplementation of Tonilisat or Roemin W2 increased (p
Abstract: The consumption of lactose in acid cheese whey
anaerobic fermentation process under fed-batch conditions was
studied. During fermentation for 100 hours the biogas production
(CO2 and CH4) was analyzed online. Among the standard analyses
FT-IR spectroscopy was used to follow the consumption of lactose by
bacteria. The absorption bands at 990, 894 and 787 cm-1 in the 2nd
derivative spectra were shown to be characteristic for lactose and
were used to follow the lactose conversion. It was shown that acid
cheese whey lactose was converted by bacteria in first 7 hours. In the
spectra of 17, 18 and 95 hour fermentation samples lactose was not
identified and these results correlated with the HPLC data.
Abstract: Lectins have a good scope in current clinical
microbiology research. In the present study evaluated the
antimicrobial activities of a D-galactose binding lectin (PnL) was
purified from the annelid, Perinereis nuntia (polychaeta) by affinity
chromatography. The molecular mass of the lectin was determined to
be 32 kDa as a single polypeptide by SDS-PAGE under both reducing
and non-reducing conditions. The hemagglutinating activity of the
PnL showed against trypsinized and glutaraldehyde-fixed human
erythrocytes was specifically inhibited by D-Gal, GalNAc,
Galβ1-4Glc and Galα1-6Glc. PnL was evaluated for in vitro
antibacterial screening studies against 11 gram-positive and
gram-negative microorganisms. From the screening results, it was
revealed that PnL exhibited significant antibacterial activity against
gram-positive bacteria. Bacillus megaterium showed the highest
growth inhibition by the lectin (250 μg/disc). However, PnL did not
inhibit the growth of gram-negative bacteria such as Vibrio cholerae
and Pseudomonas sp. PnL was also examined for in vitro antifungal
activity against six fungal phytopathogens. PnL (100 μg/mL) inhibited
the mycelial growth of Alternaria alternata (24.4%). These results
indicate that future findings of lectin applications obtained from
annelids may be of importance to life sciences.
Abstract: The present study has been taken to explore the
screening of in vitro antimicrobial activities of D-galactose-binding
sponge lectin (HOL-30). HOL-30 was purified from the marine
demosponge Halichondria okadai by affinity chromatography. The
molecular mass of the lectin was determined to be 30 kDa with a
single polypeptide by SDS-PAGE under non-reducing and reducing
conditions. HOL-30 agglutinated trypsinized and glutaraldehydefixed
rabbit and human erythrocytes with preference for type O
erythrocytes. The lectin was subjected to evaluation for inhibition of
microbial growth by the disc diffusion method against eleven human
pathogenic gram-positive and gram-negative bacteria. The lectin
exhibited strong antibacterial activity against gram-positive bacteria,
such as Bacillus megaterium and Bacillus subtilis. However, it did
not affect against gram-negative bacteria such as Salmonella typhi
and Escherichia coli. The largest zone of inhibition was recorded of
Bacillus megaterium (12 in diameter) and Bacillus subtilis (10 mm in
diameter) at a concentration of the lectin (250 μg/disc). On the other
hand, the antifungal activity of the lectin was investigated against six
phytopathogenic fungi based on food poisoning technique. The lectin
has shown maximum inhibition (22.83%) of mycelial growth of
Botrydiplodia theobromae at a concentration of 100 μg/mL media.
These findings indicate that the lectin may be of importance to
clinical microbiology and have therapeutic applications.
Abstract: A study was conducted to determine the effect of
feeding glycerol on dairy cows performance. Twenty four Holstein
Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in
early lactation; averaging 13+2.4 kg of milk, 64+45 days in milk,
55+16 months old and 325+26 kg live weight, were stratified for
milk yield, days in milk, age, stage of lactation and body weight, and
then randomly allocated to three treatment groups. All cows were fed
approximate 8 kg of concentrate together with ad libitum corn silage
and freely access to clean water. Nil or 150 and 300g of glycerol
were supplemented to the cows according to treatment groups. All
cows consumed similar concentrate, corn silage and total DM and
NELP. There were no significant differences in DM intake, CP intake,
NELP intake, milk and milk composition yields. All cows had similar
fat, protein, lactose, solid not fat and total solid percentage. All cows
gain similar live weight. The present study indicated that,
supplementation of glycerol did not enhance milk yield, milk
composition and live weight change.