Abstract: With the advance in wireless networking, IEEE 802.16 WiMAX technology has been widely deployed for several applications such as “last mile" broadband service, cellular backhaul, and high-speed enterprise connectivity. As a result, military employed WiMAX as a high-speed wireless connection for data-link because of its point to multi-point and non-line-of-sight (NLOS) capability for many years. However, the risk of using WiMAX is a critical factor in some sensitive area of military applications especially in ammunition manufacturing such as solid propellant rocket production. The US DoD policy states that the following certification requirements are met for WiMAX: electromagnetic effects on the environment (E3) and Hazards of Electromagnetic Radiation to Ordnance (HERO). This paper discuses the Recommended Power Densities and Safe Separation Distance (SSD) for HERO on WiMAX systems deployed on solid propellant rocket production. The result of this research found that WiMAX is safe to operate at close proximity distances to the rocket production based on AF Guidance Memorandum immediately changing AFMAN 91-201.
Abstract: A 2-Degrees of freedom powered prosthetic wrist
actuator has been proposed that can provide the Abduction/Adduction
& Flexion/Extension movements of the human wrist. The basic
structure of the actuator is a Ball and Socket joint and the force is
transmitted from the DC geared servo motors to the joint through the
Bowden cables. The proposed design is capable of providing the
required DOF in both axes i.e. 85° & 90° in flexion extension axis.
The size and weight of the actuator lies within the ranges of an
average human being-s wrist.
Abstract: The research study evaluated the performance of
irrigation system by using special scientific tools like Remote
Sensing and GIS technology, so that proper measurements could be
taken for the sustainable agriculture and water management.
Different performance evaluation parameters had been calculated for
the purposed data was gathered from field investigation and different
government and private organizations. According to the calculations,
organic matter ranges from 0.19% (low value) to 0.76% (high value).
In flat irrigation system for wheat yield ranges from 3347.16 to
5260.39 kg/ha, while the total water applied to wheat crop ranges
from 252.94 to 279.19 mm and WUE ranges from 13.07 to 18.37
kg/ha/mm. For rice yield ranges from 3347.47 to 5433.07 kg/ha with
total water supplied to rice crop ranges from 764.71 to 978.15 mm
and WUE ranges from 3.49 to 5.71 kg/ha/mm. Similarly, in raised
bed system wheat yield ranges from 4569.13 to 6008.60 kg/ha, total
water supplied ranges from 158.87 to 185.09 mm and WUE ranges
from 27.20 to 33.54 kg/ha/mm while in rice crop, yield ranges from
5285.04 to 6716.69 kg/ha, total water supplied ranges from 600.72 to
755.06 mm and WUE ranges from 6.41 to 10.05 kg/ha/mm. Almost
51.3% water saving is observed in bed irrigation system as compared
to flat system. Less water supplied to beds is more affective as its
WUE value is higher than flat system where more water is supplied
in both the seasons. Similarly, RWS values show that maximum
water deficit while minimum area is getting adequate water supply.
Greater yield is recorded in bed system as plant per square meter is
more in bed system in comparison of flat system Thus, the integration
of GIS tools to regularly compute performance indices could provide
irrigation managers with the means for managing efficiently the
irrigation system.
Abstract: The main goal of microarray experiments is to quantify the expression of every object on a slide as precisely as possible, with a further goal of clustering the objects. Recently, many studies have discussed clustering issues involving similar patterns of gene expression. This paper presents an application of fuzzy-type methods for clustering DNA microarray data that can be applied to typical comparisons. Clustering and analyses were performed on microarray and simulated data. The results show that fuzzy-possibility c-means clustering substantially improves the findings obtained by others.
Abstract: In this study, inhibition of Microcystis aeruginosa by
antialgal alleochemical gramine, was studied by analyzing algal
metabolic activity (represented by esterase and total dehydrogenase
activities) and cell ultrastructure (showing morphological and
ultrastructure alterations using transmission electron microscopy and
DNA ladder analysis). After gramine exposure, esterase and total
dehydrogenase activities were increased firstly but decreased later. In
contrast with the controls, the cells exposed to gramine showed
apparent ultrastructure alterations with thylakoids in breakage,
phycobilins in decrease, lipid and cyanophycin granules abundant
firstly but dissolved afterwards, DNA in fragementation. The
occurrence of increase of metabolic activity and specific granules
reflected that the resistance of cellular response to gramine was
initiated. DNA fragementation associated with the increase of
metabolic activity and specific granules hinted that gramine caused M.
aeruginosa cells to initiate some morphotype of programmed cell
death.
Abstract: A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 - kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide α- factor, under the control of AOX1 promoter. The recombined plasmid was transformed into the host strain P. pastoris KM71H and X33 by electroporation. Both host strains could efficiently express and secret phytase. The multicopy strains were screened for over expression of phytase. All the selected multicopy strains of P. pastoris X33 were examined for phytase activity, the maximum phytase yield of 1329 IU/ml was obtained after 4 days of incubation in medium BMM. The recombinant protein with MW of 97.4 KW showed to be the only one protein secreted in the culture broth. Multicopy transformant P. pastoris X33 supposed to be potential candidate for producing the commercial preparation of phytase.
Abstract: Spectrum is a scarce commodity, and considering the spectrum scarcity faced by the wireless-based service providers led to high congestion levels. Technical inefficiencies from pooled, since all networks share a common pool of channels, exhausting the available channels will force networks to block the services. Researchers found that cognitive radio (CR) technology may resolve the spectrum scarcity. A CR is a self-configuring entity in a wireless networking that senses its environment, tracks changes, and frequently exchanges information with their networks. However, CRN facing challenges and condition become worst while tracks changes i.e. reallocation of another under-utilized channels while primary network user arrives. In this paper, channels or resource reallocation technique based on DNA-inspired computing algorithm for CRN has been proposed.
Abstract: Geometry optimizations of metal complexes of Salen(bis(Salicylidene)1,2-ethylenediamine) were carried out at HF and DFT methods employing Lanl2DZ basis set. In this work structural, energies, bond lengths and other physical properties between Mn2+,Cu2+ and Ni2+ ions coordinated by salen–type ligands are examined. All calculations were performed using Gaussian 98W program series. To investigate local aromaticities, NICS were calculated at all centers of rings. The higher the band gap indicating a higher global aromaticity. The possible binding energies have been evaluated. We have evaluated Frequencies and Zero-point energy with freq calculation. The NICS(Nucleous Independent Chemical Shift) Results show Ni(II) complexes are antiaromatic and aromaticites of Mn(II) complexes are larger than Cu(II) complexes. The energy Results show Cu(II) complexes are stability than Mn(II) and Ni(II) complexes.
Abstract: In the present era of aviation technology, autonomous navigation and control have emerged as a prime area of active research. Owing to the tremendous developments in the field, autonomous controls have led today’s engineers to claim that future of aerospace vehicle is unmanned. Development of guidance and navigation algorithms for an unmanned aerial vehicle (UAV) is an extremely challenging task, which requires efforts to meet strict, and at times, conflicting goals of guidance and control. In this paper, aircraft altitude and heading controllers and an efficient algorithm for self-governing navigation using MATLAB® mapping toolbox is presented which also enables loitering of a fixed wing UAV over a specified area. For this purpose, a nonlinear mathematical model of a UAV is used. The nonlinear model is linearized around a stable trim point and decoupled for controller design. The linear controllers are tested on the nonlinear aircraft model and navigation algorithm is subsequently developed for for autonomous flight of the UAV. The results are presented for trajectory controllers and waypoint based navigation. Our investigation reveals that MATLAB® mapping toolbox can be exploited to successfully deliver an efficient algorithm for autonomous aerial navigation for a UAV.
Abstract: A series of microarray experiments produces observations
of differential expression for thousands of genes across multiple
conditions.
Principal component analysis(PCA) has been widely used in
multivariate data analysis to reduce the dimensionality of the data in
order to simplify subsequent analysis and allow for summarization of
the data in a parsimonious manner. PCA, which can be implemented
via a singular value decomposition(SVD), is useful for analysis of
microarray data.
For application of PCA using SVD we use the DNA microarray
data for the small round blue cell tumors(SRBCT) of childhood
by Khan et al.(2001). To decide the number of components which
account for sufficient amount of information we draw scree plot.
Biplot, a graphic display associated with PCA, reveals important
features that exhibit relationship between variables and also the
relationship of variables with observations.
Abstract: Deaminated lesions were produced via nitrosative oxidation of natural nucleobases; uracul (Ura, U) from cytosine (Cyt, C), hypoxanthine (Hyp, H) from adenine (Ade, A), and xanthine (Xan, X) and oxanine (Oxa, O) from guanine (Gua, G). Such damaged nucleobases may induce mutagenic problems, so that much attentions and efforts have been poured on the revealing of their mechanisms in vivo or in vitro. In this study, we employed these deaminated lesions as useful probes for analysis of DNA-binding/recognizing proteins or enzymes. Since the pyrimidine lesions such as Hyp, Oxa and Xan are employed as analogues of guanine, their comparative uses are informative for analyzing the role of Gua in DNA sequence in DNA-protein interaction. Several DNA oligomers containing such Hyp, Oxa or Xan substituted for Gua were designed to reveal the molecular interaction between DNA and protein. From this approach, we have got useful information to understand the molecular mechanisms of the DNA-recognizing enzymes, which have not ever been observed using conventional DNA oligomer composed of just natural nucleobases.
Abstract: Noise has adverse effect on human health and
comfort. Noise not only cause hearing impairment, but it also acts as
a causal factor for stress and raising systolic pressure. Additionally it
can be a causal factor in work accidents, both by marking hazards
and warning signals and by impeding concentration. Industry
workers also suffer psychological and physical stress as well as
hearing loss due to industrial noise. This paper proposes an approach
to enable engineers to point out quantitatively the noisiest source for
modification, while multiple machines are operating simultaneously.
The model with the point source and spherical radiation in a free field
was adopted to formulate the problem. The procedure works very
well in ideal cases (point source and free field). However, most of the
industrial noise problems are complicated by the fact that the noise is
confined in a room. Reflections from the walls, floor, ceiling, and
equipment in a room create a reverberant sound field that alters the
sound wave characteristics from those for the free field. So the model
was validated for relatively low absorption room at NIT Kurukshetra
Central Workshop. The results of validation pointed out that the
estimated sound power of noise sources under simultaneous
conditions were on lower side, within the error limits 3.56 - 6.35 %.
Thus suggesting the use of this methodology for practical
implementation in industry. To demonstrate the application of the
above analytical procedure for estimating the sound power of noise
sources under simultaneous operating conditions, a manufacturing
facility (Railway Workshop at Yamunanagar, India) having five
sound sources (machines) on its workshop floor is considered in this
study. The findings of the case study had identified the two most
effective candidates (noise sources) for noise control in the Railway
Workshop Yamunanagar, India. The study suggests that the
modification in the design and/or replacement of these two identified
noisiest sources (machine) would be necessary so as to achieve an
effective reduction in noise levels. Further, the estimated data allows
engineers to better understand the noise situations of the workplace
and to revise the map when changes occur in noise level due to a
workplace re-layout.
Abstract: This paper examines the depiction of Muslim militants in Thai newspapers in 2004. Stuart Hall-s “representation" and “public idioms" are used as theoretical frameworks. Critical Discourse Analysis is employed as a methodology to examine 240 news articles from two leading Thai language newspapers. The results show that the militants are usually labeled as “southern bandits." This suggests that they are just a culprit of the violence in the deep south of Thailand. They are usually described as people who cause turbulence. Consequently, the military have to get rid of them. However, other aspects of the groups such as their political agenda or the failures of the Thai state in dealing with the Malay Muslims were not mention in the news stories. In the time of violence, the researcher argues that this kind of newspaper coverage may help perpetuate the discourse of Malay Muslim, instead of providing fuller picture of the ongoing conflicts.
Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: Bio-chips are used for experiments on genes and
contain various information such as genes, samples and so on. The
two-dimensional bio-chips, in which one axis represent genes and the
other represent samples, are widely being used these days. Instead of
experimenting with real genes which cost lots of money and much
time to get the results, bio-chips are being used for biological
experiments. And extracting data from the bio-chips with high
accuracy and finding out the patterns or useful information from such
data is very important. Bio-chip analysis systems extract data from
various kinds of bio-chips and mine the data in order to get useful
information. One of the commonly used methods to mine the data is
classification. The algorithm that is used to classify the data can be
various depending on the data types or number characteristics and so
on. Considering that bio-chip data is extremely large, an algorithm that
imitates the ecosystem such as the ant algorithm is suitable to use as an
algorithm for classification. This paper focuses on finding the
classification rules from the bio-chip data using the Ant Colony
algorithm which imitates the ecosystem. The developed system takes
in consideration the accuracy of the discovered rules when it applies it
to the bio-chip data in order to predict the classes.
Abstract: A Watson-Crick automaton is recently introduced as a
computational model of DNA computing framework. It works on
tapes consisting of double stranded sequences of symbols. Symbols
placed on the corresponding cells of the double-stranded sequences are
related by a complimentary relation. In this paper, we investigate a
variation of Watson-Crick automata in which both heads read the tape
in reverse directions. They are called reverse Watson-Crick finite
automata (RWKFA). We show that all of following four classes, i.e.,
simple, 1-limited, all-final, all-final and simple, are equal to
non-restricted version of RWKFA.
Abstract: Type 2 diabetes mellitus (T2DM) is a complex
metabolic disorder that characterized by the presence of high glucose
in blood that cause from insulin resistance and insufficiency due to
deterioration β-cell Langerhans functions. T2DM is commonly
caused by the combination of inherited genetic variations as well as
our own lifestyle. Metallothionein (MT) is a known cysteine-rich
protein responsible in helping zinc homeostasis which is important in
insulin signaling and secretion as well as protection our body from
reactive oxygen species (ROS). MT scavenged ROS and free
radicals in our body happen to be one of the reasons of T2DM and its
complications. The objective of this study was to investigate the
association of MT1A and MT2A polymorphisms between T2DM and
control subjects among Malay populations. This study involved 150
T2DM and 120 Healthy individuals of Malay ethnic with mixed
genders. The genomic DNA was extracted from buccal cells and
amplified for MT1A and MT2A loci; the 347bp and 238bp banding
patterns were respectively produced by mean of the Polymerase
Chain Reaction (PCR). The PCR products were digested with Mlucl
and Tsp451 restriction enzymes respectively and producing
fragments lengths of (158/189/347bp) and (103/135/238bp)
respectively. The ANOVA test was conducted and it shown that there
was a significant difference between diabetic and control subjects for
age, BMI, WHR, SBP, FPG, HBA1C, LDL, TG, TC and family
history with (P0.05). The genotype
frequency for AA, AG and GG of MT1A polymorphisms was 72.7%,
22.7% and 4.7% in cases and 15%, 55% and 30% in control
respectively. As for MT2A, genotype frequency of GG, GC and CC
was 42.7%, 27.3% and 30% in case and 5%, 40% and 55% for
control respectively. Both polymorphisms show significant difference
between two investigated groups with (P=0.000). The Post hoc test
was conducted and shows a significant difference between the
genotypes within each polymorphism (P=0. 000). The MT1A and
MT2A polymorphisms were believed to be the reliable molecular
markers to distinguish the T2DM subjects from healthy individuals in
Malay populations.
Abstract: Authentication of multimedia contents has gained much attention in recent times. In this paper, we propose a secure semi-fragile watermarking, with a choice of two watermarks to be embedded. This technique operates in integer wavelet domain and makes use of semi fragile watermarks for achieving better robustness. A self-recovering algorithm is employed, that hides the image digest into some Wavelet subbands to detect possible malevolent object manipulation undergone by the image (object replacing and/or deletion). The Semi-fragility makes the scheme tolerant for JPEG lossy compression as low as quality of 70%, and locate the tempered area accurately. In addition, the system ensures more security because the embedded watermarks are protected with private keys. The computational complexity is reduced using parameterized integer wavelet transform. Experimental results show that the proposed scheme guarantees the safety of watermark, image recovery and location of the tempered area accurately.
Abstract: Artemia is one of the most conspicuous invertebrates
associated with aquaculture. It can be considered as a model
organism, offering numerous advantages for comprehensive and
multidisciplinary studies using morphologic or molecular methods.
Since DNA extraction is an important step of any molecular
experiment, a new and a rapid method of DNA extraction from adult
Artemia was described in this study. Besides, the efficiency of this
technique was compared with two widely used alternative techniques,
namely Chelex® 100 resin and SDS-chloroform methods. Data
analysis revealed that the new method is the easiest and the most cost
effective method among the other methods which allows a quick and
efficient extraction of DNA from the adult animal.
Abstract: Unified Theory of Acceptance and Use of Technology
(UTAUT) model has demonstrated the influencing factors for generic
information systems use such as tablet personal computer (TPC) and
mobile communication. However, in the context of digital library
system, there has been very little effort to determine factors affecting
the intention to use digital library based on the UTAUT model. This
paper investigates factors that are expected to influence the intention
of postgraduate students to use digital library based on modified
UTAUT model. The modified model comprises of constructs
represented by several latent variables, namely performance
expectancy (PE), effort expectancy (EE), information quality (IQ)
and service quality (SQ) and moderated by age, gender and
experience in using digital library. Results show that performance
expectancy, effort expectancy and information quality are positively
related to the intention to use digital library, while service quality is
negatively related to the intention to use digital library. Age and
gender have shown no evidence of any significant interactions, while
experience in using digital library significantly interacts with effort
expectancy and intention to use digital library. This has provided the
evidence of a moderating effect of experience in the intention to use
digital library. It is expected that this research will shed new lights
into research of acceptance and intention to use the library in a digital
environment.