Abstract: According to FDA (Food and Drug Administration of the United States), vinegar is definedas a sour liquid containing at least 4 grams acetic acid in 100 cubic centimeter (4% solution of acetic acid) of solution that is produced from sugary materials by alcoholic fermentation. In the base of microbial starters, vinegars could be contained of more than 50 types of volatile and aromatic substances that responsible for their sweet taste and smelling. Recently the vinegar industry has a great proportion in agriculture, food and microbial biotechnology. The acetic acid bacteria are from the family Acetobacteraceae. Regarding to the latest version of Bergy-s Mannual of Systematic Bacteriology that has categorized bacteria in the base of their 16s RNA differences, the most important acetic acid genera are included Acetobacter (genus I), Gluconacetobacter (genus VIII) and Gluconobacter (genus IX). The genus Acetobacter that is primarily used in vinegar manufacturing plants is a gram negative, obligate aerobe coccus or rod shaped bacterium with the size 0.6 - 0.8 X 1.0 - 4.0 μm, nonmotile or motile with peritrichous flagella and catalase positive – oxidase negative biochemically. Some strains are overoxidizer that could convert acetic acid to carbon dioxide and water.In this research one Acetobacter native strain with high acetic acid productivity was isolated from Iranian white – red cherry. We used two specific culture media include Carr medium [yeast extract, 3%; ethanol, 2% (v/v); bromocresol green, 0.002%; agar, 2% and distilled water, 1000 ml], Frateur medium [yeast extract, 10 g/l; CaCO3, 20 g/l; ethanol, 20 g/l; agar, 20 g/l and distilled water, 1000 ml] and an industrial culture medium. In addition to high acetic acid production and high growth rate, this strain had a good tolerance against ethanol concentration that was examined using modified Carr media with 5%, 7% and 9% ethanol concentrations. While the industrial strains of acetic acid bacteria grow in the thermal range of 28 – 30 °C, this strain was adapted for growth in 34 – 36 °C after 96 hours incubation period. These dramatic characteristics suggest a potential biotechnological strain in production of cherry vinegar with a sweet smell and different nutritional properties in comparison to recent vinegar types. The lack of growth after 24, 48 and 72 hours incubation at 34 – 36 °C and the growth after 96 hours indicates a good and fast thermal flexibility of this strain as a significant characteristic of biotechnological and industrial strains.
Abstract: In this study, we investigated the effects of ginger and
L-carnitine on the reproductive performance of male rats with respect
to semen parameters, male sex hormones and the testicular
antioxidant system. A total of sixty mature male albino rats were
divided into four groups of fifteen rats. The control group received
saline, whereas the other three groups received ginger (100 mg kg-1 d-
1.), L-carnitine (150 mg kg-1 d-1.) or a combination of both ginger
(100 mg kg-1 d-1.) and L-carnitine (150 mg kg-1 d-1.) via a stomach
tube daily for one month. At the end of the treatment period, the rats
were sacrificed, and their sperm characteristics (count, motility and
viability), antioxidant enzyme factors levels (reduced glutathione,
catalase, superoxide dismutase and total antioxidant capacity) and sex
hormone levels (testosterone, Follicle stimulating hormone(FSH) and
luteinizing hormone (LH) were analysed. Our results showed that the
three experimental treatments improved sperm parameters,
antioxidant enzyme activity and testosterone hormone levels; the
most pronounced positive effects were observed in the group that
received a combination of both ginger and L-carnitine. Therefore, the
administration of a combination of ginger and L-carnitine may be
beneficial for improving male sexual performance.
Abstract: Kombucha Tea Ferment (KT), was given to male
albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks
before intraperitoneal administration of 3.5 mg/Kg body weight
CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks
after each treatment. Hepatic and nephritic pathological changes
included significant increases of serum alanine transaminase (ALT),
aspartate transaminase (AST), and alkaline phosphatase (ALP)
activities, and creatinine and urea contents with significant decrease
in serum total antioxidant capacity (TAC). Increase in oxidative
stress markers in liver and kidney tissues expressed by significant
increase in malondialdehyde (MDA) and nitric oxide (NO) contents
associated to significant depletion in superoxide dismutase (SOD)
and catalase (CAT) activities, and reduced glutathione (GSH) content
were recorded. KT administration results in recovery of all the
pathological changes. It could be concluded that KT might protect
liver and kidney from oxidative damage induced by exposure to
cadmium and/ or γ-irradiation.
Abstract: The use of bioindicators plants (lichens, bryophytes
and Sphagnum....) in monitoring pollution by heavy metals has been
the subject of several works. However, few studies have addressed
the impact of specific type-s pollutants (fertilizers, pesticides.) on
these organisms.
We propose in this work to make the highlighting effect of NPKs
(NPK: nitrogen-phosphate-potassium-sulfate (NP2O5K2O) (15,15,15),
at concentrations of 10, 20, 30 , 40 and 50mM/L) on the activity of
detoxification enzymes (GSH/GST, CAT, APX and MDA) of plant
bioindicators (mosses and lichens) after treatment for 3 and 7 days.
This study shows the important role of the defense system in the
accumulation and tolerance to chemical pollutants through the
activation of enzymatic (GST (glutathione-S-transferase, APX
(ascorbat peroxidase), CAT (catalase)) and nonenzymatic biomarkers
(GSH (glutathione), MDA (malondialdehyde)) against oxidative
stress generated by the NPKs.
Abstract: This study aimed to detect and to identify the main
strains of airborne microorganisms present in the Shanghai Metro
system. Samples were collected using agar plates exposed to the air
and microorganisms were identified using catalase, plasma coagulase
and hymolytic analysis. The results show that the concentration of
mildew present within a newly opened metro line was significantly
higher than for other lines. Differences among underground and
elevated stations can be attributed to differences in passenger flow and
the environment surrounding the stations. Additionally, the
investigation indicated that bacteria reached maximum levels at
different times on weekdays and weekends. The bacteria in the Metro
stations were identified as primarily Gram positive, consisting mainly
of coagulase-negative staphylococcus strains (CNS).