Abstract: In this study Vitaton (an organic supplement which
contains fermentative β-carotene) and synthetic astaxanthin
(CAROPHYLL® Pink) were evaluated as pro-growth factors in
Rainbow trout diet. An 8 week feeding trial was conducted to
determine the effects of Vitaton versus astaxanthin on rearing factors,
survival rate and visual flesh color of Rainbow trout
(Oncorhnchynchus mykiss) with initial weight of 196±5. Four
practical diets were formulated to contain 50 and 80 (ppm) of β-
carotene and astaxanthin and also a control diet was prepared without
any pigment. Each diet was fed to triplicate groups of fish rearing in
fresh water. Fish were fed twice daily. The water temperature
fluctuated from 12 to 15 (C˚) and also dissolved oxygen content was
between 7 to 7.5 (mg/lit) during the experimental period. At the end
of the experiment, growth and food utilization parameters and
survival rate were unaffected by dietary treatments (p>0.05). Also,
there was no significant difference between carcass yield within
treatments (p>0.05). No significant difference recognized between
visual flesh color (SalmoFan score) of fish fed Vitaton-containing
diets. On the contrary, feeding on diets containing 50 and 80 (ppm)
of astaxanthin, increased SalmoFan score (flesh astaxanthin
concentration) from
Abstract: This study aims to screen out and to optimize the
major nutrients for maximum carotenoid production and
antioxidation characteristics by Rhodotorula rubra. It was found that
supplementary of 10 g/l glucose as carbon source, 1 g/l ammonium
sulfate as nitrogen source and 1 g/l yeast extract as growth factor in
the medium provided the better yield of carotenoid content of 30.39
μg/g cell dry weight the amount of antioxidation of Rhodotorula
rubra by DPPH, ABTS and MDA method were 1.463%, 34.21% and
34.09 μmol/l, respectively.
Abstract: Experiments with pumpkin-rowanberry marmalade
candies were carried out at the Faculty of Food Technology of the
Latvia University of Agriculture. The objective of this investigation
was to evaluate the quality changes of pumpkin-rowanberry
marmalade candies packed in different packaging materials during
the storage of 15 weeks, and to find the most suitable packaging
material for prolongation of low sugar marmalade candies shelf-life.
An active packaging in combination with modified atmosphere
(MAP, CO2 100%) was examined and compared with traditional
packaging in air ambiance. Polymer Multibarrier 60 and paper bags
were used. Influence of iron based oxygen absorber in sachets of 500
cc obtained from Mitsubishi Gas Chemical Europe Ageless® on the
marmalade candies’ quality was tested during shelf life. Samples of
80±5 g were packaged in polymer pouches (110 mm x 110 mm),
hermetically sealed by MULTIVAC C300 vacuum chamber machine,
and stored in a room temperature +21±0.5 °C. The physiochemical
properties –moisture content, hardness, aw, pH, changes of
atmosphere content (CO2 and O2), ascorbic acid, total carotenoids,
total phenols in headspace of packs, and microbial conditions were
analysed before packaging and in the 1st, 3rd , 5th, 8th, 11th and 15th
weeks of storage.
Abstract: Lutein is a dietary oxycarotenoid which is found
to reduce the risks of Age-related Macular Degeneration
(AMD). Supercritical fluid extraction of lutein esters from
marigold petals was carried out and was found to be much
effective than conventional solvent extraction. The
saponification of pre-concentrated lutein esters to produce free
lutein was studied which showed a composition of about 88%
total carotenoids (UV-VIS spectrophotometry) and 90.7%
lutein (HPLC). The lipase catalyzed hydrolysis of lutein esters
in conventional medium was investigated. The optimal
temperature, pH, enzyme concentration and water activity
were found to be 50°C, 7, 15% and 0.33 respectively and the
activity loss of lipase was about 25% after 8 times re-use in at
50°C for 12 days. However, the lipase catalyzed hydrolysis of
lutein esters in conventional media resulted in poor
conversions (16.4%).
Abstract: To evaluate genetic variation of wheat (Triticum
aestivum) affected by heat and drought stress on eight Australian
wheat genotypes that are parents of Doubled Haploid (HD) mapping
populations at the vegetative stage, the water stress experiment was
conducted at 65% field capacity in growth room. Heat stress
experiment was conducted in the research field under irrigation over
summer. Result show that water stress decreased dry shoot weight
and RWC but increased osmolarity and means of Fv/Fm values in all
varieties except for Krichauff. Krichauff and Kukri had the
maximum RWC under drought stress. Trident variety was shown
maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield
and Fv/Fm 0.815 under water stress condition. However, the
recovery of quantum yield was apparent between 4 to 7 days after
stress in all varieties. Nevertheless, increase in water stress after that
lead to strong decrease in quantum yield. There was a genetic
variation for leaf pigments content among varieties under heat stress.
Heat stress decreased significantly the total chlorophyll content that
measured by SPAD. Krichauff had maximum value of Anthocyanin
content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and
chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b
(0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content
belonged to Kukri. The quantum yield of all varieties decreased
significantly, when the weather temperature increased from 28 ÔùªC to
36 ÔùªC during the 6 days. However, the recovery of quantum yield
was apparent after 8th day in all varieties. The maximum decrease
and recovery in quantum yield was observed in Krichauff. Drought
and heat tolerant and moderately tolerant wheat genotypes were
included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut
and Excalibur were clustered into most sensitive and moderately
sensitive genotypes. Finally, the results show that there was a
significantly genetic variation among the eight varieties that were
studied under heat and water stress.
Abstract: Milk from differently fed cows (supplemented with carotenoids from carrots or palm oil product Carotino CAF 100) was obtained in a conventional dairy farm to assess the carotenoid potential to protect milk fat against oxidation. The extracted anhydrous milk fat (AMF) was tested by peroxide value, and Rancimat tests. Temperature, and light stimulation for reaction acceleration was used. The oxidative stability enhancement by carotenoids was detected in peroxide value test – the strongest effect was observed in palm oil, following by carrot supplemented group, compared to control group, whose feed was unchanged. Rancimat accelerated oxidation test results did not show any superiority of the oxidative stability of the AMF samples from milk of the carotenoidsupplemented cow groups. The average oxidation stability of AMF dark-stored samples was 12.59 ± 0.294 h, and it was significantly (p < 0.05) higher than that of AMF light-affected samples, i.e. 2.60 ± 0.191 h.
Abstract: Application of pesticides in the paddy fields has
deleterious effects on non-target organisms including cyanobacteria
which are photosynthesizing and nitrogen fixing micro-organisms
contributing significantly towards soil fertility and crop yield.
Pesticide contamination in the paddy fields has manifested into a
serious global environmental concern. To study the effect of one such
pesticide, three cyanobacterial strains; Anabaena fertilissima,
Aulosira fertilissima and Westiellopsis prolifica were selected for
their stress responses to an Organochlorine insecticide - 6, 7, 8, 9, 10,
10-hexachloro-1, 5, 5a, 6, 9, 9a-hexahydro-6, 9-methano-2, 4, 3-
benzodioxathiepine-3-oxide, with reference to their photosynthesic
pigments-chlorophyll-a and carotenoids as well as accessory
pigments-phycobiliproteins (phycocyanin, allophycocyanin and
phycoerythrin), stress induced biochemical metabolites like
carbohydrates, proteins, amino acids, phenols and enzymes-nitrate
reductase, glutamine synthetase and succinate dehydrogenase. All
the three cyanobacterial strains were adversely affected by the
insecticide doses and inhibition was dose dependent. Reduction in
photosynthetic and accessory pigments, metabolites, nitrogen fixing
and respiratory enzymes of the test organisms were accompanied
with an initial increase in their total protein at lower Organochlorine
doses. On the other hand, increased amount of phenols in all the
insecticide treated concentrations was indicative of stressed activities
of the organisms.