Abstract: Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Abstract: Continuation of an active call is one of the most important quality measurements in the cellular systems. Handoff process enables a cellular system to provide such a facility by transferring an active call from one cell to another. Different approaches are proposed and applied in order to achieve better handoff service. The principal parameters used to evaluate handoff techniques are: forced termination probability and call blocking probability. The mechanisms such as guard channels and queuing handoff calls decrease the forced termination probability while increasing the call blocking probability. In this paper we present an overview about the issues related to handoff initiation and decision and discuss about different types of handoff techniques available in the literature.
Abstract: In this paper, the application of GRNN in
modeling of SOFC fuel cells were studied. The parameters
are of interested as voltage and power value and the current
changes are investigated. In addition, the comparison between
GRNN neural network application and conventional method
was made. The error value showed the superlative results.
Abstract: The counting and analysis of blood cells allows the
evaluation and diagnosis of a vast number of diseases. In particular,
the analysis of white blood cells (WBCs) is a topic of great interest to
hematologists. Nowadays the morphological analysis of blood cells is
performed manually by skilled operators. This involves numerous
drawbacks, such as slowness of the analysis and a nonstandard
accuracy, dependent on the operator skills. In literature there are only
few examples of automated systems in order to analyze the white
blood cells, most of which only partial. This paper presents a
complete and fully automatic method for white blood cells
identification from microscopic images. The proposed method firstly
individuates white blood cells from which, subsequently, nucleus and
cytoplasm are extracted. The whole work has been developed using
MATLAB environment, in particular the Image Processing Toolbox.
Abstract: A new technique based on Pattern search optimization is proposed for estimating different solar cell parameters in this paper. The estimated parameters are the generated photocurrent, saturation current, series resistance, shunt resistance, and ideality factor. The proposed approach is tested and validated using double diode model to show its potential. Performance of the developed approach is quite interesting which signifies its potential as a promising estimation tool.
Abstract: Today, cancer remains one of the major diseases that
lead to death. The main obstacle in chemotherapy as a main cancer
treatment is the toxicity to normal cells due to Multidrug Resistance
(MDR) after the use of anticancer drugs. Proposed solution to
overcome this problem is the use of MDR efflux inhibitor of cinchona
alkaloids which is delivered together with anticancer drugs
encapsulated in the form of polymeric nanoparticles. The particles
were prepared by the hydration method. The characterization of
nanoparticles was particle size, zeta potential, entrapment efficiency
and in vitro drug release. Combination nanoparticle size ranged 29-45
nm with a neutral surface charge. Entrapment efficiency was above
87% for the use quinine, quinidine or cinchonidine in combination
with etoposide. The release test results exhibited that the cinchona
alkaloids release released faster than that of etoposide. Collectively,
cinchona alkaloids can be packaged along with etoposide in
nanomicelles for better cancer therapy.
Abstract: In today-s global and competitive market,
manufacturing companies are working hard towards improving their
production system performance. Most companies develop production
systems that can help in cost reduction. Manufacturing systems
consist of different elements including production methods,
machines, processes, control and information systems. Human issues
are an important part of manufacturing systems, yet most companies
do not pay sufficient attention to them. In this paper, a workforce
planning (WP) model is presented. A non-linear programming model
is developed in order to minimize the hiring, firing, training and
overtime costs. The purpose is to determine the number of workers
for each worker type, the number of workers trained, and the number
of overtime hours. Moreover, a decision support system (DSS) based
on the proposed model is introduced using the Excel-Lingo software
interfacing feature. This model will help to improve the interaction
between the workers, managers and the technical systems in
manufacturing.
Abstract: This paper explores the opportunity of using tri-axial
wireless accelerometers for supervised monitoring of sports
movements. A motion analysis system for the upper extremities of
lawn bowlers in particular is developed. Accelerometers are placed
on parts of human body such as the chest to represent the shoulder
movements, the back to capture the trunk motion, back of the hand,
the wrist and one above the elbow, to capture arm movements. These
sensors placement are carefully designed in order to avoid restricting
bowler-s movements. Data is acquired from these sensors in soft-real
time using virtual instrumentation; the acquired data is then
conditioned and converted into required parameters for motion
regeneration. A user interface was also created to facilitate in the
acquisition of data, and broadcasting of commands to the wireless
accelerometers. All motion regeneration in this paper deals with the
motion of the human body segment in the X and Y direction, looking
into the motion of the anterior/ posterior and lateral directions
respectively.
Abstract: In the context of global climate change, flooding and sea level rise is increasingly threatening coastal urban areas, in which large population is continuously concentrated. Dutch experiences in urban water system management provide high reference value for sustainable coastal urban development projects. Preliminary studies shows the urban water system in Almere, a typical Dutch polder city, have three kinds of operational modes, achieving functions as: (1) coastline control – strong multiple damming system prevents from storm surges and maintains sufficient capacity upon risks; (2) high flexibility – large area and widely scattered open water system greatly reduce local runoff and water level fluctuation; (3) internal water maintenance – weir and sluice system maintains relatively stable water level, providing excellent boating and landscaping service, coupling with water circulating model maintaining better water quality. Almere has provided plenty of hints and experiences for ongoing development of coastal cities in emerging economies.
Abstract: A five-class density histogram with an index named cumulative density was proposed to analyze the short-term HRV. 150 subjects participated in the test, falling into three groups with equal numbers -- the healthy young group (Young), the healthy old group (Old), and the group of patients with congestive heart failure (CHF). Results of multiple comparisons showed a significant differences of the cumulative density in the three groups, with values 0.0238 for Young, 0.0406 for Old and 0.0732 for CHF (p
Abstract: By employing BS (Base Station) cooperation we can
increase substantially the spectral efficiency and capacity of cellular
systems. The signals received at each BS are sent to a central unit that
performs the separation of the different MT (Mobile Terminal) using
the same physical channel. However, we need accurate sampling and
quantization of those signals so as to reduce the backhaul
communication requirements.
In this paper we consider the optimization of the quantizers for BS
cooperation systems. Four different quantizer types are analyzed and
optimized to allow better SQNR (Signal-to-Quantization Noise
Ratio) and BER (Bit Error Rate) performance.
Abstract: The most important parameter in transformers life
expectancy is the hot-spot temperature level which accelerates the
rate of aging of the insulation. The aim of this paper is to present
thermal models for transformers loaded at prefabricated MV/LV
transformer substations and outdoor situations. The hot-spot
temperature of transformers is studied using their top-oil temperature
rise models. The thermal models proposed for hot-spot and top-oil
temperatures of different operating situations are compared. Since the
thermal transfer is different for indoor and outdoor transformers
considering their operating conditions, their hot-spot thermal models
differ from each other. The proposed thermal models are verified by
the results obtained from the experiments carried out on a typical
1600 kVA, 30 /0.4 kV, ONAN transformer for both indoor and
outdoor situations.
Abstract: Median filter is widely used to remove impulse noise
without blurring sharp edges. However, when noise level increased,
or with thin edges, median filter may work poorly. This paper
proposes a new filter, which will detect edges along four possible
directions, and then replace noise corrupted pixel with estimated
noise-free edge median value. Simulations show that the proposed
multi-stage directional median filter can provide excellent
performance of suppressing impulse noise in all situations.
Abstract: In this paper, we study a class of serially concatenated block codes (SCBC) based on matrix interleavers, to be employed in fixed wireless communication systems. The performances of SCBC¬coded systems are investigated under various interleaver dimensions. Numerical results reveal that the matrix interleaver could be a competitive candidate over conventional block interleaver for frame lengths of 200 bits; hence, the SCBC coding based on matrix interleaver is a promising technique to be employed for speech transmission applications in many international standards such as pan-European Global System for Mobile communications (GSM), Digital Cellular Systems (DCS) 1800, and Joint Detection Code Division Multiple Access (JD-CDMA) mobile radio systems, where the speech frame contains around 200 bits.
Abstract: The least mean square (LMS) algorithmis one of the
most well-known algorithms for mobile communication systems
due to its implementation simplicity. However, the main limitation
is its relatively slow convergence rate. In this paper, a booster
using the concept of Markov chains is proposed to speed up the
convergence rate of LMS algorithms. The nature of Markov
chains makes it possible to exploit the past information in the
updating process. Moreover, since the transition matrix has a
smaller variance than that of the weight itself by the central limit
theorem, the weight transition matrix converges faster than the
weight itself. Accordingly, the proposed Markov-chain based
booster thus has the ability to track variations in signal
characteristics, and meanwhile, it can accelerate the rate of
convergence for LMS algorithms. Simulation results show that the
LMS algorithm can effectively increase the convergence rate and
meantime further approach the Wiener solution, if the
Markov-chain based booster is applied. The mean square error is
also remarkably reduced, while the convergence rate is improved.
Abstract: The effects of enzyme action and heat pretreatment on oil extraction yield from sunflower kernels were analysed using hexane extraction with Soxhlet, and aqueous extraction with incubator shaker. Ground kernels of raw and heat treated kernels, each with and without Viscozyme treatment were used. Microscopic images of the kernels were taken to analyse the visible effects of each treatment on the cotyledon cell structure of the kernels. Heat pretreated kernels before both extraction processes produced enhanced oil extraction yields than the control, with steam explosion the most efficient. In hexane extraction, applying a combination of steam explosion and Viscozyme treatments to the kernels before the extraction gave the maximum oil extractable in 1 hour; while for aqueous extraction, raw kernels treated with Viscozyme gave the highest oil extraction yield. Remarkable cotyledon cell disruption was evident in kernels treated with Viscozyme; whereas steam explosion and conventional heat treated kernels had similar effects.
Abstract: Bone remodeling occurs by the balanced action of
bone resorbing osteoclasts (OC) and bone-building osteoblasts.
Increased bone resorption by excessive OC activity contributes
to malignant and non-malignant diseases including osteoporosis.
To study OC differentiation and function, OC formed in
in vitro cultures are currently counted manually, a tedious
procedure which is prone to inter-observer differences. Aiming
for an automated OC-quantification system, classification of
OC and precursor cells was done on fluorescence microscope
images based on the distinct appearance of fluorescent nuclei.
Following ellipse fitting to nuclei, a combination of eight
features enabled clustering of OC and precursor cell nuclei.
After evaluating different machine-learning techniques, LOGREG
achieved 74% correctly classified OC and precursor cell
nuclei, outperforming human experts (best expert: 55%). In
combination with the automated detection of total cell areas,
this system allows to measure various cell parameters and most
importantly to quantify proteins involved in osteoclastogenesis.
Abstract: In this paper, we propose a novel improvement for the generalized Lloyd Algorithm (GLA). Our algorithm makes use of an M-tree index built on the codebook which makes it possible to reduce the number of distance computations when the nearest code words are searched. Our method does not impose the use of any specific distance function, but works with any metric distance, making it more general than many other fast GLA variants. Finally, we present the positive results of our performance experiments.
Abstract: The effect of antifungal compound from Bacillus
subtilis strain LB5 was tested against conidial germination of
Colletotrichum gloeosporioides and Pestalotiopsis eugeniae, causal
agent of anthracnose and fruit rot of wax apple, respectively.
Observation under scanning electron microscope and light compound
microscope revealed that conidial germination was completely
inhibited when treated with culture broth, culture filtrate, or crude
extract from strain LB5. Identification of purified antifungal
compound produced by strain LB5 in cell-free supernatant by nuclear
magnetic resonance and fast atom bombardment showed that the
active compound was iturin A-2.
Abstract: The increasing influence of traffic on building
objects and people residing in them should be taken into account in
diagnosis and design. Users of buildings expect that vibrations
occurring in their environment, will not only lead to damage to the
building or its accelerated wear, but neither would affect the required
comfort in rooms designed to accommodate people. This article
describes the methods and principles useful in designing and building
diagnostics located near transportation routes, with particular
emphasis on the impact of traffic vibration on people in buildings. It
also describes the procedures used in obtaining information about the
parameters of vibrations in different cases of diagnostics and design.
A universal algorithm of procedure in diagnostics and design of
buildings taking into account assurance of human vibration comfort
of people residing in the these buildings was presented.