Abstract: Direct and indirect somatic embryogenesis (SE) from
petiole and leaf explants of Scaevola aemula R. Br. cv. 'Purple
Fanfare' was achieved. High frequency of somatic embryos was
obtained directly from petiole and leaf explants using an inductive
plant growth regulator signal thidiazuron (TDZ). Petiole explants
were more responsive to SE than leaves. Plants derived from somatic
embryos of petiole explants germinated more readily into plants. SE
occurred more efficiently in half-strength Murashige and Skoog
(MS) medium than in full-strength MS medium. Non-embryogenic
callus induced by 2, 4-dichlorophenoxyacetic acid was used to
investigate the feasibility of obtaining SE with TDZ as a secondary
inductive plant growth regulator (PGR) signal. Non-embryogenic
callus of S. aemula was able to convert into an “embryogenic
competent mode" with PGR signal. Protocol developed for induction
of direct and indirect somatic embryogenesis in S. aemula can
improve the large scale propagation system of the plant in future.
Abstract: Three alumina-supported Pt-Sn catalysts have been
prepared by means of co-impregnation and characterized by XRD and
N2 adsorption. The influence of catalyst composition and reaction
conditions on the conversion and selectivity were investigated in the
hydrogenation of acetic acid in an isothermal integral fixed bed
reactor. The experiments were performed on the temperature interval
468-548 K, liquid hourly space velocity (LHSV) of 0.3-0.7h-1,
pressures between 1.0 and 5.0Mpa. A good compromise of
0.75%Pt-1.5%Sn can act as an optimized acetic acid hydrogenation
catalyst, and the conversion and selectivity can be tuned through the
variation of reaction conditions.
Abstract: For stricter drinking water regulations in the future, reducing the humic acid and disinfection byproducts in raw water, namely, trihalomethanes (THMs) and haloacetic acids (HAAs) is worthy for research. To investigate the removal of waterborne organic material using a lab-scale of bio-activated carbon filter under different EBCT, the concentrations of humic acid prepared were 0.01, 0.03, 0.06, 0.12, 0.17, 0.23, and 0.29 mg/L. Then we conducted experiments using a pilot plant with in-field of the serially connected bio-activated carbon filters and hollow fiber membrane processes employed in traditional water purification plants. Results showed under low TOC conditions of humic acid in influent (0.69 to 1.03 mg TOC/L) with an EBCT of 30 min, 40 min, and 50 min, TOC removal rates increases with greater EBCT, attaining about 39 % removal rate. The removal rate of THMs and HAAs by BACF was 54.8 % and 89.0 %, respectively.
Abstract: Rice bran has been abandoned as agricultural waste for million tonnes per year in Thailand, therefore they have been proposed to be utilized as a rich carbon source in the production of bioethanol. Many toxic compounds are possibly released during the pretreatment of rice bran prior the fermentation process. This study aims to analyze on the availability of toxic compounds and the amount of glucose obtained from 2 different pretreatments using sulfuric acid and mixed cellulase enzymes (without and with delignification/ activated charcoal). The concentration of furfural, 5- hydroxymethyl furfural (5-HMF), levulinic acid, vanillin, syringaldehyde and4-hydroxybenzaldehyde (4-HB) and the percent acetic acid were found to be 0.0517 ± 0.049 mg/L, 0.032 ± 0.06 mg/L, 21074 ± 1685.62 mg/L, 126.265 ± 6.005 mg/L, 2.89 ± 0.30 mg/L, 0.37 ± 0.031mg/L and 0.72% under the pretreatment process without delignification/ activated charcoal treatment and 384.47 ± 99.02 g/L, 0.068 mg/L, 142107.62 ± 8664.6 mg/L, 0.19 mg/L, 5.43 ± 3.29 mg/L, 4.80 ± 0.76 mg/L and 0.254% under the pretreatment process with delignification/ activated charcoal treatment respectively. The presence of high concentration of acetic acid was found to impede the growth of Zymomonas mobilis strain TISTR 551 despite the present of high concentration of levulinic acid. Z. mobilis strain TISTR 551 was found to produce 8.96 ± 4.06 g/L of ethanol under 4 days fementation period in biofilm stage in which represented 40% theoretical yield.
Abstract: Sol-gel method has been used to fabricate
nanocomposite films on glass substrates composed halloysite clay
mineral and nanocrystalline TiO2. The methodology for the synthesis
involves a simple chemistry method utilized nonionic surfactant
molecule as pore directing agent along with the acetic acid-based solgel
route with the absence of water molecules. The thermal treatment
of composite films at 450oC ensures elimination of organic material
and lead to the formation of TiO2 nanoparticles onto the surface of
the halloysite nanotubes. Microscopy techniques and porosimetry
methods used in order to delineate the structural characteristics of the
materials. The nanocomposite films produced have no cracks and
active anatase crystal phase with small crystallite size were deposited
on halloysite nanotubes. The photocatalytic properties for the new
materials were examined for the decomposition of the Basic Blue 41
azo dye in solution. These, nanotechnology based composite films
show high efficiency for dye’s discoloration in spite of different
halloysite quantities and small amount of halloysite/TiO2 catalyst
immobilized onto glass substrates. Moreover, we examined the
modification of the halloysite/TiO2 films with silver particles in order
to improve the photocatalytic properties of the films. Indeed, the
presence of silver nanoparticles enhances the discoloration rate of the
Basic Blue 41 compared to the efficiencies obtained for unmodified
films.
Abstract: Three batches of yogurts were made with soy protein
isolate (SPI) supplemented with 2% (S2), 4% (S4) or 6% (S6) of
skim milk powder (SMP). The fourth batch (control; S0) was
prepared from SPI without SMP supplementation. Lactobacillus
delbrueckii ssp. bulgaricus ATCC 11842 (Lb 11842) and
Streptococcus thermophilus ST 1342 (ST 1342) were used as the
starter culture. Biotransformation of the inactive forms, isoflavone
glycosides (IG) to biologically active forms, isoflavone aglycones
(IA), was determined during 28 d storage. The viability of both
microorganisms was significantly higher (P < 0.05) in S2, S4, and S6
than that in S0. The ratio of lactic acid/acetic acid in S0 was in the
range of 15.53 – 22.31 compared to 7.24 – 12.81 in S2, S4 and S6.
The biotransformation of IG to IA in S2, S4 and S6 was also
enhanced by 9.9 -13.3% compared to S0.
Abstract: Chemically defined Schlegel-s medium was modified
to improve production of cell growth and other metabolites that are
produced by fluorescent pseudomonad R62 strain. The modified
medium does not require pH control as pH changes are kept within ±
0.2 units of the initial pH 7.1 during fermentation. The siderophore
production was optimized for the fluorescent pseudomonad strain in
the modified medium containing 1% glycerol as a major carbon
source supplemented with 0.05% succinic acid and 0.5% Ltryptophan.
Indole-3 acetic acid (IAA) production was higher when
L-tryptophan was used at 0.5%. The 2,4- diacetylphloroglucinol
(DAPG) was higher with amended three trace elements in medium.
The optimized medium produced 2.28 g/l of dry cell mass and 900
mg/l of siderophore at the end of 36 h cultivation, while the
production levels of IAA and DAPG were 65 mg/l and 81 mg/l
respectively at the end of 48 h cultivation.