Abstract: A major goal in animal genetics is to understand the role of common genetic variants in diseases susceptibility and production traits. Sahiwal cattle can be considered as a global animal genetic resource due to its relatively high milk producing ability, resistance against tropical diseases and heat tolerant. CYP11B1 gene provides instructions for making a mitochondrial enzyme called steroid 11-beta-hydroxylase. It catalyzes the 11deoxy-cortisol to cortisol and 11deoxycorticosterone to corticosterone in cattle. The bovine CYP11B1 gene is positioned on BTA14q12 comprises of eight introns and nine exons and protein is associated with mitochondrial epithelium. The present study was aimed to identify the single-nucleotide polymorphisms in CYP11B1 gene in Sahiwal cattle breed of Pakistan. Four polymorphic sites were identified in exon one of CYP11B1 gene through sequencing approach. Significant finding was the incidence of the C→T polymorphism in 5'-UTR, causing amino acid substitution from alanine to valine (A30V) in Sahiwal cattle breed. That Ala/Val polymorphism may serve as a powerful genetic tool for the development of DNA markers that can be used for the particular traits for different local cattle breeds.
Abstract: The purpose of present work was to study the drying kinetics of whole acorn and its kernel at different drying air temperatures and their effective moisture diffusivity. The results indicated that the drying time of whole acorn was 442, 206 and 188 min at the air temperature of 65, 75 and 85ºC, respectively. At the same temperatures, the drying time of kernel was 131, 56 and 76min. The results showed that the effect of drying air temperature increasing on the drying time reduction could not be significant on acorn drying at all conditions. The effective moisture diffusivity of whole acorn and kernel increased with increasing air temperature from 65 to 75ºC. However more air temperature increasing, led to decreasing this property of acorn kernel. The critical temperature of acorn drying was about 75°C in which acorn kernel had the highest effective moisture diffusivity.
Abstract: The heat and mass transfer was investigated during the falling rate period of spray drying of a slurry droplet. The effect of the porosity of crust layer formed from primary particles during liquid evaporation was studied numerically using the developed mathematical model which takes into account the heat and mass transfer in the core and crust regions, the movement of the evaporation interface, and the external heat and mass transfer between the drying air and the droplet surface. It was confirmed that the heat transfer through the crust layer was more intense in the case of the dense droplet than the loose one due to the enhanced thermal conduction resulting in the higher average droplet temperature. The mass transfer was facilitated in the crust layer of loose droplet owing to the large pore space available for diffusion of water vapor from the evaporation interface to the outer droplet surface. The longer drying time is required for the droplet of high porosity to reach the final moisture content than that for the dense one due to the larger amount of water to be evaporated during the falling rate.
Abstract: Intercalation imparts interesting features to the host graphite material. Two different types of intercalated compounds called (GIC-bisulphate or Nyex 1000 and GIC-nitrate or Nyex 3000) were tested for their adsorption capacity and ability to undergo electrochemical regeneration. It was found that Nyex 3000 showed comparatively slow kinetics along with reduced adsorption capacity to one half for acid violet 17 as adsorbate. Acid violet 17 was selected as model organic pollutant for evaluating comparative performance of said adsorbents. Both adsorbent materials showed 100% regeneration efficiency as achieved by passing a charge of 36 C g-1 at a current density of 12 mA cm-2 and a treatment time of 60 min.
Abstract: Creative design requires new approaches to assessment
in vocational and technological education. To date, there has been little
discussion on instruments used to evaluate dies produced by students
in vocational and technological education. Developing a generic
instrument has been very difficult due to the diversity of creative
domains, the specificity of content, and the subjectivity involved in
judgment. This paper presents an instrument for measuring the
creativity in the design of products by expanding the Consensual
Assessment Technique (CAT). The content-based scale was evaluated
for content validity by 5 experts. The scale comprises 5 criteria:
originality; practicability; precision; aesthetics; and exchangeability.
Nine experts were invited to evaluate the dies produced by 38 college
students who enrolled in a Product Design and Development course.
To further explore the degree of rater agreement, inter-rater reliability
was calculated for each dimension using Kendall's coefficient of
concordance test. The inter-judge reliability scores achieved
significance, with coefficients ranging from 0.53 to 0.71.
Abstract: In-situ chemical oxidation (ISCO) has been widely
used for source zone remediation of Dense Nonaqueous Phase
Liquids (DNAPLs) in subsurface environments. DNAPL source
zones for karst aquifers are generally located in epikarst where the
DNAPL mass is trapped either in karst soil or at the regolith contact
with carbonate bedrock. This study aims to investigate the
performance of oxidation of residual trichloroethylene found in such
environments by potassium permanganate. Batch and flow cell
experiments were conducted to determine the kinetics and the mass
removal rate of TCE. pH change, Cl production, TCE and MnO4
destruction were monitored routinely during experiments. Nonreactive
tracer tests were also conducted prior and after the oxidation
process to determine the influence of oxidation on flow conditions.
The results show that oxidant consumption rate of the calcareous
epikarst soil was significant and the oxidant demand was determined
to be 20 g KMnO4/kg soil. Oxidation rate of residual TCE (1.26x10-3
s-1) was faster than the oxidant consumption rate of the soil (2.54 -
2.92x10-4 s-1) at only high oxidant concentrations (> 40 mM
KMnO4). Half life of TCE oxidation ranged from 7.9 to 10.7 min.
Although highly significant fraction of residual TCE mass in the
system was destroyed by permanganate oxidation, TCE
concentration in the effluent remained above its MCL. Flow
interruption tests indicate that efficiency of ISCO was limited by the
rate of TCE dissolution and the rate-limited desorption of TCE. The
residence time and the initial concentration of the oxidant in the
source zone also controlled the efficiency of ISCO in epikarst.
Abstract: A new and cost effective RP-HPLC method was
developed and validated for simultaneous analysis of non steroidal
anti inflammatory dugs Diclofenac sodium (DFS), Flurbiprofen
(FLP) and an opioid analgesic Tramadol (TMD) in advanced drug
delivery systems (Liposome and Microcapsules), marketed brands
and human plasma. Isocratic system was employed for the flow of
mobile phase consisting of 10 mM sodium dihydrogen phosphate
buffer and acetonitrile in molar ratio of 67: 33 with adjusted pH of
3.2. The stationary phase was hypersil ODS column (C18, 250×4.6
mm i.d., 5 μm) with controlled temperature of 30 C°. DFS in
liposomes, microcapsules and marketed drug products was
determined in range of 99.76-99.84%. FLP and TMD in
microcapsules and brands formulation were 99.78 - 99.94 % and
99.80 - 99.82 %, respectively. Single step liquid-liquid extraction
procedure using combination of acetonitrile and trichloroacetic acid
(TCA) as protein precipitating agent was employed. The detection
limits (at S/N ratio 3) of quality control solutions and plasma samples
were 10, 20, and 20 ng/ml for DFS, FLP and TMD, respectively.
The Assay was acceptable in linear dynamic range. All other
validation parameters were found in limits of FDA and ICH method
validation guidelines. The proposed method is sensitive, accurate and
precise and could be applicable for routine analysis in
pharmaceutical industry as well as in human plasma samples for
bioequivalence and pharmacokinetics studies.
Abstract: Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. A better knowledge of the fouling process can be obtained by controlling the formation of the first protein layer at a solid surface. A number of methods have been investigated to inhibit adsorption of proteins. In this study, the adsorption kinetics of
Abstract: Using ab initio theoretical calculations, we present
analysis of fragmentation process. The analysis is performed in two
steps. The first step is calculation of fragmentation energies by ab
initio calculations. The second step is application of the energies to
kinetic description of process. The energies of fragments are
presented in this paper. The kinetics of fragmentation process can be
described by numerical models. The method for kinetic analysis is
described in this paper. The result - composition of fragmentation
products - will be calculated in future. The results from model can be
compared to the concentrations of fragments from mass spectrum.
Abstract: The direct synthesis process of dimethyl ether (DME)
from syngas in slurry reactors is considered to be promising because
of its advantages in caloric transfer. In this paper, the influences of
operating conditions (temperature, pressure and weight hourly space
velocity) on the conversion of CO, selectivity of DME and methanol
were studied in a stirred autoclave over Cu-Zn-Al-Zr slurry catalyst,
which is far more suitable to liquid phase dimethyl ether synthesis
process than bifunctional catalyst commercially. A Langmuir-
Hinshelwood mechanism type global kinetics model for liquid phase
DME direct synthesis based on methanol synthesis models and a
methanol dehydration model has been investigated by fitting our
experimental data. The model parameters were estimated with
MATLAB program based on general Genetic Algorithms and
Levenberg-Marquardt method, which is suitably fitting experimental
data and its reliability was verified by statistical test and residual
error analysis.
Abstract: Calcite aCalcite and aragonite are the two common
polymorphs of CaCO3 observed as biominerals. It is universal that
the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM).
In vivo crystallization, Mg2+ inhibits calcite formation. For this
reason, stony corals skeleton may be formed only aragonite crystals
in the biocalcification. It is special in case of soft corals of which
formed only calcite crystal; however, this interesting phenomenon,
still uncharacterized in the marine environment, has been explored in
this study using newly purified cell-free proteins isolated from the
endoskeletal sclerites of soft coral. By recording the decline of pH in
vitro, the control of CaCO3 nucleation and crystal growth by the cellfree
proteins was revealed. Using Atomic Force Microscope, here we
find that these endoskeletal cell-free proteins significantly design the
morphological shape in the molecular-scale kinetics of crystal
formation and those proteins act as surfactants to promote ion
attachment at calcite steps.nd aragonite are the two common polymorphs of CaCO3 observed as biominerals. It is universal that the sea water contents a high Mg2+ (50mM) relative to Ca2+ (10mM). In vivo crystallization, Mg2+ inhibits calcite formation. For this reason, stony corals skeleton may be formed only aragonite crystals in the biocalcification. It is special in case of soft corals of which formed only calcite crystal; however, this interesting phenomenon, still uncharacterized in the marine environment, has been explored in this study using newly purified cell-free proteins isolated from the endoskeletal sclerites of soft coral. By recording the decline of pH in vitro, the control of CaCO3 nucleation and crystal growth by the cell-free proteins was revealed. Using Atomic Force Microscope, here we find that these endoskeletal cell-free proteins significantly design the morphological shape in the molecular-scale kinetics of crystal formation and those proteins act as surfactants to promote ion attachment at calcite steps. KeywordsBiomineralization, Calcite, Cell-free protein, Soft coral
Abstract: Essential hypertension (HTN) usually clusters with other cardiovascular risk factors such as age, overweight, diabetes, insulin resistance and dyslipidemia. The target organ damage (TOD) such as left ventricular hypertrophy, microalbuminuria (MA), acute coronary syndrome (ACS), stroke and cognitive dysfunction takes place early in course of hypertension. Though the prevalence of hypertension is high in India, the relationship between microalbuminuria and target organ damage in hypertension is not well studied. This study aim at detecting MA in essential hypertension and its relation to severity of HTN, duration of HTN, body mass index (BMI), age and TOD such as HTN retinopathy and acute coronary syndrome The present study was done in 100 patients of essential hypertension non diabetics admitted to B.L.D.E.University-s Sri B.M.Patil Medical College, Bijapur, from October 2008 to April 2011. The patients underwent detailed history and clinical examination. Early morning 5 ml of urine sample was collected & MA was estimated by immunoturbidometry method. The relationship of MA with the duration & severity of HTN, BMI, age, sex and TOD's like hypertensive retinopathy, ACS was assessed by univariate analysis. The prevalence of MA in this study was found to be 63 %. In that 42% were male & 21% were female. In this study a significant association between MA and the duration of hypertension (p = 0.036) & (OR =0.438). Longer the duration of hypertension, more possibility of microalbumin in urine. Also there was a significant association between severity of hypertension and MA (p=0.045) and (OR=0.093). MA was positive in 50 (79.4%) patients out of 63, whose blood pressure was >160/100 mm Hg. In this study a significant association between MA and the grades of hypertensive retinopathy (p =0.011) and acute coronary syndrome (p = 0.041) (OR =2.805). Gender and BMI did not pose high risk for MA in this study.The prevalence of MA in essential hypertension is high in this part of the community and MA will increase the risk of developing target organ damage.Early screening of patients with essential hypertension for MA and aggressive management of positive cases might reduce the burden of chronic kidney diseases and cardiovascular diseases in the community.
Abstract: Iron in groundwater is one of the problems that render the water unsuitable for drinking. The concentration above 0.3 mg/L is common in groundwater. The conventional method of removal is by precipitation under oxic condition. In this study, iron removal under anaerobic conditions was examined by batch experiment as a main purpose. The process involved by purging of groundwater samples with H2S to form iron sulfide. Removal up to 83% for 1 mg/L iron solution was achieved. The removal efficiency dropped to 82% and 75% for the higher initial iron concentrations 3.55 and 5.01 mg/L, respectively. The average residual sulfide concentration in water after the process was 25*g/L. The Eh level during the process was -272 mV. The removal process was found to follow the first order reaction with average rate constant of 4.52 x 10-3. The half-life for the concentrations to reduce from initial values was 157 minutes.
Abstract: Adsorption of CS2 vapors has been studied on
different types of activated carbons obtained from different source
raw materials. The activated carbons have different surface areas and
are associated with varying amounts of the carbon-oxygen surface
groups. The adsorption of CS2 vapors is not directly related to surface
area, but is considerably influenced by the presence of carbonoxygen
surface groups. The adsorption decreases on increasing the
amount of carbon-oxygen surface groups on oxidation and increases
when these surface groups are eliminated on degassing. The
adsorption is maximum in case of the 950°-degassed carbon sample
which is almost completely free of any associated oxygen. The
kinetic data as analysed by Empirical diffusion model and Linear
driving force mass transfer model indicate that the adsorption does
not involve Fickian diffusion but may be considered as a pseudo first
order mass transfer process. The activation energy of adsorption and
isosteric enthalpies of adsorption indicate that the adsorption does not
involve interaction between CS2 and carbon-oxygen surface groups,
but hydrophobic interactions between CS2 and C-C atoms in the
carbon lattice.
Abstract: The genus Fumaria L. (Papaveraceae) in Iran
comprises 8 species with a vast medicinal use in Asian folk
medicine. These herbs are considered to be useful in the
treatment of gastrointestinal disease and skin disorders.
Antioxidant activities of alkaloids and phenolic extracts of
these species had been studied previously. These species are:
F. officinalis, F. parviflora, F. asepala, F. densiflora, F.
schleicheri, F. vaillantii and F. indica. More than 50
populations of Fumaria species were sampled from nature. In
this study different fatty acids are extracted. Their picks were
recorded by GC technique. This species contain some kind of
fatty acids with antioxidant effects. A part of these lipids are
phospholipids. As these are unsaturated fatty acids they may
have industrial use as natural additive to cosmetics, dermal
and oral medicines. The presences of different materials are
discussed. Our studies for antioxidant effects of these
substances are continued.
Abstract: Impurity metals such as manganese and cadmium
from high-tenor cobalt electrolyte solution were selectively removed
by solvent extraction method using Co-D2EHPA after converting the functional group of D2EHPA with Co2+ ions. The process parameters
such as pH, organic concentration, O/A ratio, kinetics etc. were
investigated and the experiments were conducted by batch tests in the laboratory bench scale. Results showed that a significant amount
of manganese and cadmium can be extracted using Co-D2EHPA for the optimum processing of cobalt electrolyte solution at equilibrium pH about 3.5. The McCabe-Thiele diagram, constructed from the
extraction studies showed that 100% impurities can be extracted through four stages for manganese and three stages for cadmium
using O/A ratio of 0.65 and 1.0, respectively. From the stripping study, it was found that 100% manganese and cadmium can be stripped from the loaded organic using 0.4 M H2SO4 in a single
contact. The loading capacity of Co-D2EHPA by manganese and cadmium were also investigated with different O/A ratio as well as
with number of stages of contact of aqueous and organic phases. Valuable information was obtained for the designing of an impurities
removal process for the production of pure cobalt with less trouble in the electrowinning circuit.
Abstract: Single crystals of Magnesium alloys such as pure Mg,
Mg-1Zn-0.5Y, Mg-0.1Y, and Mg-0.1Ce alloys were successfully
fabricated in this study by employing the modified Bridgman method.
To determine the exact orientation of crystals, pole figure
measurement using X-ray diffraction were carried out on each single
crystal. Hardness and compression tests were conducted followed by
subsequent recrysatllization annealing. Recrystallization kinetics of
Mg alloy single crystals has been investigated. Fabricated single
crystals were cut into rectangular shaped specimen and solution
treated at 400oC for 24 hrs, and then deformed in compression mode
by 30% reduction. Annealing treatment for recrystallization has been
conducted on these cold-rolled plates at temperatures of 300oC for
various times from 1 to 20 mins. The microstructure observation and
hardness measurement conducted on the recrystallized specimens
revealed that static recrystallization of ternary alloy single crystal was
very slow, while recrystallization behavior of binary alloy single
crystals appeared to be very fast.
Abstract: Protein-protein interactions (PPI) play a crucial role in many biological processes such as cell signalling, transcription, translation, replication, signal transduction, and drug targeting, etc. Structural information about protein-protein interaction is essential for understanding the molecular mechanisms of these processes. Structures of protein-protein complexes are still difficult to obtain by biophysical methods such as NMR and X-ray crystallography, and therefore protein-protein docking computation is considered an important approach for understanding protein-protein interactions. However, reliable prediction of the protein-protein complexes is still under way. In the past decades, several grid-based docking algorithms based on the Katchalski-Katzir scoring scheme were developed, e.g., FTDock, ZDOCK, HADDOCK, RosettaDock, HEX, etc. However, the success rate of protein-protein docking prediction is still far from ideal. In this work, we first propose a more practical measure for evaluating the success of protein-protein docking predictions,the rate of first success (RFS), which is similar to the concept of mean first passage time (MFPT). Accordingly, we have assessed the ZDOCK bound and unbound benchmarks 2.0 and 3.0. We also createda new benchmark set for protein-protein docking predictions, in which the complexes have experimentally determined binding affinity data. We performed free energy calculation based on the solution of non-linear Poisson-Boltzmann equation (nlPBE) to improve the binding mode prediction. We used the well-studied thebarnase-barstarsystem to validate the parameters for free energy calculations. Besides,thenlPBE-based free energy calculations were conducted for the badly predicted cases by ZDOCK and ZRANK. We found that direct molecular mechanics energetics cannot be used to discriminate the native binding pose from the decoys.Our results indicate that nlPBE-based calculations appeared to be one of the promising approaches for improving the success rate of binding pose predictions.
Abstract: The ability of pomelo peel, a natural biosorbent, to remove Cd(II) ions from aqueous solution by biosorption was investigated. The experiments were carried out by batch method at 25 °C. The influence of solution pH, initial cadmium ion concentrations and contact times were evaluated. Cadmium ion removal increased significantly as the pH of the solution increased from pH 1 to pH 5. At pH 5, the cadmium ion removal reached a maximum value. The equilibrium process was described well by the Langmuir isotherm model, with a maximum biosorption capacity of 21.83 mg/g. The biosorption was relatively quick, (approx. 20 min). Biosorption kinetics followed a pseudo-second-order model. The result showed that pomelo peel was effective as a biosorbent for removing cadmium ions from aqueous solution. It is a low cost material that shows potential to be applied in wastewater technology for remediation of heavy metal contamination.
Abstract: Verapamil has been shown to inhibit fentanyl uptake in vitro and is a potent P-glycoprotein inhibitor. Tissue partitioning of loperamide, a commercially available opioid, is closely controlled by the P-gp efflux transporter. The following studies were designed to evaluate the effect of opioids on verapamil partitioning in the lung and brain, in vivo. Opioid (fentanyl or loperamide) was administered by intravenous infusion to Sprague Dawley rats alone or in combination with verapamil and plasma, with lung and brain tissues were collected at 1, 5, 6, 8, 10 and 60 minutes. Drug dispositions were modeled by recirculatory pharmacokinetic models. Fentanyl slightly increased the verapamil lung (PL) partition coefficient yet decreased the brain (PB) partition coefficient. Furthermore, loperamide significantly increased PLand PB. Fentanyl reduced the verapamil volume of distribution (V1) and verapamil elimination clearance (ClE). Fentanyl decreased verapamil brain partitioning, yet increased verapamil lung partitioning. Also, loperamide increased lung and brain partitioning in vivo. These results suggest that verapamil and fentanyl may be substrates of an unidentified inward transporter in brain tissue and confirm that verapamil and loperamide are substrates of the efflux transporter P-gp.