Abstract: In the immunologic sense, clinical infection is a state
of failure of the immune system to combat the pathogenic weapon of
the bacteria invading the host. A motile gram negative vibroid
organism associated with marked mono and poly nuclear cell
responses was traced during the examination of a clinical material
from an infected common carp Cyprinus carpio. On primary plate
culture, growth was shown to be pure, dense population of an
Aeromonas-like colony morphotype. The pure isolate was found to
be; Aerobic, facultatively anaerobic, non-halophilic, grew at 0C, and
37C, oxidase positive utilizes glucose through fermentative pathway,
resist 0/129 and novobiocin, produces alanine and lysine
decarboxylases but non-producing ornithine dehydrolases. Tests for
the in vitro determinants of pathogenicity has shown to be; Betahaemolytic
onto blood agar, gelatinase, casienase and amylase
producer. Three in vivo determinants of pathogenicity were tested as,
the lethal dose fifty, the pathogenesis and pathogenicity. It was
evident that 0.1 milliliter of the causal bacterial cell suspension of a
density 1 x 107 CFU/ml injected intramuscularly into an average of
100gms fish toke five days incubation period, then at the day six
morbidity and mortality were initiated. LD50 was recorded at the day
12 post-infection. Use of an LD50 doses to study the pathogenicity,
reveals mononuclear and polynuclear cell responses, on examining
the stained direct films of the clinical materials from the
experimentally infected fish. Re-isolation tests confirm that the reisolant
is same. The course of the infection in natural case was shown
manifestation of; skin ulceration, haemorrhage and descaling. On
evisceration, the internal organs were shown; congestion in the
intestines, spleen and, air sacs. The induced infection showed a
milder form of these manifestations. The grading of the virulence of
this organism was virulent causing chronic course of infections as
indicated from the pathogenesis and pathogenicity studies. Thus the
infectious bacteria were consistent with Aeromonas hydrophila, and
the infection was chronic.
Abstract: This paper presents the design and characterization of
analog readout interface circuits for ion sensitive field effect
transistor (ISFET) and ion selective electrode (ISE) based sensor.
These interface circuits are implemented using MIMOS’s 0.35um
CMOS technology and experimentally characterized under 24-leads
QFN package. The characterization evaluates the circuit’s
functionality, output sensitivity and output linearity. Commercial
sensors for both ISFET and ISE are employed together with glass
reference electrode during testing. The test result shows that the
designed interface circuits manage to readout signals produced by
both sensors with measured sensitivity of ISFET and ISE sensor are
54mV/pH and 62mV/decade, respectively. The characterized output
linearity for both circuits achieves above 0.999 Rsquare. The readout
also has demonstrated reliable operation by passing all qualifications
in reliability test plan.
Abstract: Purpose: The study aimed to assess the depressant or
antidepressant effects of several Nonsteroidal Anti-Inflammatory
Drugs (NSAIDs) in mice: the selective cyclooxygenase-2 (COX-2)
inhibitor meloxicam, and the non-selective COX-1 and COX-2
inhibitors lornoxicam, sodium metamizole, and ketorolac. The
current literature data regarding such effects of these agents are
scarce.
Materials and methods: The study was carried out on NMRI mice
weighing 20-35 g, kept in a standard laboratory environment. The
study was approved by the Ethics Committee of the University of
Medicine and Pharmacy „Carol Davila”, Bucharest. The study agents
were injected intraperitoneally, 10 mL/kg body weight (bw) 1 hour
before the assessment of the locomotor activity by cage testing (n=10
mice/ group) and 2 hours before the forced swimming tests (n=15).
The study agents were dissolved in normal saline (meloxicam,
sodium metamizole), ethanol 11.8% v/v in normal saline (ketorolac),
or water (lornoxicam), respectively. Negative and positive control
agents were also given (amitryptilline in the forced swimming test).
The cage floor used in the locomotor activity assessment was divided
into 20 equal 10 cm squares. The forced swimming test involved
partial immersion of the mice in cylinders (15/9cm height/diameter)
filled with water (10 cm depth at 28C), where they were left for 6
minutes. The cage endpoint used in the locomotor activity assessment
was the number of treaded squares. Four endpoints were used in the
forced swimming test (immobility latency for the entire 6 minutes,
and immobility, swimming, and climbing scores for the final 4
minutes of the swimming session), recorded by an observer that was
„blinded” to the experimental design. The statistical analysis used the
Levene test for variance homogeneity, ANOVA and post-hoc
analysis as appropriate, Tukey or Tamhane tests.
Results: No statistically significant increase or decrease in the
number of treaded squares was seen in the locomotor activity
assessment of any mice group. In the forced swimming test,
amitryptilline showed an antidepressant effect in each experiment, at
the 10 mg/kg bw dosage. Sodium metamizole was depressant at 100
mg/kg bw (increased the immobility score, p=0.049, Tamhane test),
but not in lower dosages as well (25 and 50 mg/kg bw). Ketorolac
showed an antidepressant effect at the intermediate dosage of 5
mg/kg bw, but not so in the dosages of 2.5 and 10 mg/kg bw,
respectively (increased the swimming score, p=0.012, Tamhane test).
Meloxicam and lornoxicam did not alter the forced swimming
endpoints at any dosage level.
Discussion: 1) Certain NSAIDs caused changes in the forced
swimming patterns without interfering with locomotion. 2) Sodium
metamizole showed a depressant effect, whereas ketorolac proved
antidepressant. Conclusion: NSAID-induced mood changes are not
class effects of these agents and apparently are independent of the
type of inhibited cyclooxygenase (COX-1 or COX-2).
Disclosure: This paper was co-financed from the European Social
Fund, through the Sectorial Operational Programme Human Resources Development 2007-2013, project number POSDRU /159
/1.5 /S /138907 "Excellence in scientific interdisciplinary research,
doctoral and postdoctoral, in the economic, social and medical fields
-EXCELIS", coordinator The Bucharest University of Economic
Studies.
Abstract: This paper presents the development of a robot car
that can track the motion of an object by detecting its color through
an Android device. The employed computer vision algorithm uses the
OpenCV library, which is embedded into an Android application of a
smartphone, for manipulating the captured image of the object. The
captured image of the object is subjected to color conversion and is
transformed to a binary image for further processing after color
filtering. The desired object is clearly determined after removing
pixel noise by applying image morphology operations and contour
definition. Finally, the area and the center of the object are
determined so that object’s motion to be tracked. The smartphone
application has been placed on a robot car and transmits by Bluetooth
to an Arduino assembly the motion directives so that to follow
objects of a specified color. The experimental evaluation of the
proposed algorithm shows reliable color detection and smooth
tracking characteristics.
Abstract: Reliability allocation is quite important during early
design and development stages for a system to apportion its specified
reliability goal to subsystems. This paper improves the reliability
fuzzy allocation method, and gives concrete processes on determining
the factor and sub-factor sets, weight sets, judgment set, and
multi-stage fuzzy evaluation. To determine the weight of factor and
sub-factor sets, the modified trapezoidal numbers are proposed to
reduce errors caused by subjective factors. To decrease the fuzziness
in fuzzy division, an approximation method based on linear
programming is employed. To compute the explicit values of fuzzy
numbers, centroid method of defuzzification is considered. An
example is provided to illustrate the application of the proposed
reliability allocation method based on fuzzy arithmetic.
Abstract: Interaction of Schiff base complexes of Iron and
Manganese: Iron [N, N’ Bis (5- (triphenyl phosphonium methyl)
salicylidene) -1, 2 ethanediamine) chloride, [Fe Salen]Cl; Manganese
[N, N’ Bis (5- (triphenyl phosphonium methyl) salicylidene) -1, 2
ethanediamine) acetate, were investigated by spectroscopic and
isothermal titration calorimetry techniques (ITC).
The absorbance spectra of complexes have shown hyper and
hypochromism in the presence of DNA that is indication of
interaction of complexes with DNA. The linear dichroism (LD)
measurements confirmed the bending of DNA in the presence of
complexes.
Furthermore, Isothermal titration calorimetry experiments
approved that complexes bound to DNA on the base of both
electrostatic and hydrophobic interactions. More, ITC profile exhibits
the existence of two binding phases for the complexes. Antibacterial
activity of ligand and complexes were tested in vitro to evaluate their
activity against the gram positive and negative bacteria.
Abstract: In this paper, we used data mining to extract
biomedical knowledge. In general, complex biomedical data
collected in studies of populations are treated by statistical methods,
although they are robust, they are not sufficient in themselves to
harness the potential wealth of data. For that you used in step two
learning algorithms: the Decision Trees and Support Vector Machine
(SVM). These supervised classification methods are used to make the
diagnosis of thyroid disease. In this context, we propose to promote
the study and use of symbolic data mining techniques.
Abstract: Lipases constitute one of the most important groups of
industrial enzymes that catalyze the hydrolysis of triacylglycerol to
glycerol and fatty acids. Muscarinic antagonist relieves smooth
muscle spasm of the gastrointestinal tract and effect on the
cardiovascular system. In this research the effect of a muscarinic
antagonist on the lipase activity of Pseudomonas aeruginosa was
studied. Lineweaver–Burk plot showed that the drug inhibited the
enzyme by competitive inhibition. The IC50 value (0.16 mM) and Ki
(0.03 mM) of the drug revealed the drug bound to enzyme with high
affinity. Determination of enzyme activity in various pH and
temperature showed that the maximum activity of lipase was at pH 8
and 60oC both in presence and absence of the drug.
Abstract: Natural hydrocarbon seepage has helped petroleum
exploration as a direct indicator of gas and/or oil subsurface
accumulations. Surface macro-seeps are generally an indication of a
fault in an active Petroleum Seepage System belonging to a Total
Petroleum System. This paper describes a case study in which
multiple analytical techniques were used to identify and characterize
trace petroleum-related hydrocarbons and other volatile organic
compounds in groundwater samples collected from Sousse aquifer
(Central Tunisia). The analytical techniques used for analyses of
water samples included gas chromatography-mass spectrometry (GCMS),
capillary GC with flame-ionization detection, Compound
Specific Isotope Analysis, Rock Eval Pyrolysis. The objective of the
study was to confirm the presence of gasoline and other petroleum
products or other volatile organic pollutants in those samples in order
to assess the respective implication of each of the potentially
responsible parties to the contamination of the aquifer. In addition,
the degree of contamination at different depths in the aquifer was also
of interest. The oil and gas seeps have been investigated using
biomarker and stable carbon isotope analyses to perform oil-oil and
oil-source rock correlations. The seepage gases are characterized by
high CH4 content, very low δ13CCH4 values (-71,9 ‰) and high
C1/C1–5 ratios (0.95–1.0), light deuterium–hydrogen isotope ratios (-
198 ‰) and light δ13CC2 and δ13CCO2 values (-23,8‰ and-23,8‰
respectively) indicating a thermogenic origin with the contribution of
the biogenic gas. An organic geochemistry study was carried out on
the more ten oil seep samples. This study includes light hydrocarbon
and biomarkers analyses (hopanes, steranes, n-alkanes, acyclic
isoprenoids, and aromatic steroids) using GC and GC-MS. The
studied samples show at least two distinct families, suggesting two
different types of crude oil origins: the first oil seeps appears to be
highly mature, showing evidence of chemical and/or biological
degradation and was derived from a clay-rich source rock deposited
in suboxic conditions. It has been sourced mainly by the lower
Fahdene (Albian) source rocks. The second oil seeps was derived
from a carbonate-rich source rock deposited in anoxic conditions,
well correlated with the Bahloul (Cenomanian-Turonian) source rock.
Abstract: Diminished antioxidant defense or increased
production of reactive oxygen species in the biological system can
result in oxidative stress which may lead to various
neurodegenerative diseases including Alzheimer’s disease (AD).
Microglial activation also contributes to the progression of AD by
producing several proinflammatory cytokines, nitric oxide (NO) and
prostaglandin E2 (PGE2). Oxidative stress and inflammation have
been reported to be possible pathophysiological mechanisms
underlying AD. In addition, the cholinergic hypothesis postulates that
memory impairment in patient with AD is also associated with the
deficit of cholinergic function in the brain. Although a number of
drugs have been approved for the treatment of AD, most of these
synthetic drugs have diverse side effects and yield relatively modest
benefits. Marine algae have great potential in pharmaceutical and
biomedical applications as they are valuable sources of bioactive
properties such as anticoagulation, antimicrobial, antioxidative,
anticancer and anti-inflammatory. Hence, this study aimed to provide
an overview of the properties of Malaysian seaweeds (Padina
australis, Sargassum polycystum and Caulerpa racemosa) in
inhibiting oxidative stress, neuroinflammation and cholinesterase
enzymes. These seaweeds significantly exhibited potent DPPH and
moderate superoxide anion radical scavenging ability (P
Abstract: The influences of cell-free solutions (CFSs) of lactic
acid bacteria (LAB) on cadaverine and other biogenic amines
production by Listeria monocytogenes and Staphylococcus aureus
were investigated in lysine decarboxylase broth (LDB) using HPLC.
Cell free solutions were prepared from Lactococcus lactis subsp.
lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus
acidilactici and Streptococcus thermophiles. Two different
concentrations that were 50% and 25% CFS and the control without
CFSs were prepared. Significant variations on biogenic amine
production were observed in the presence of L. monocytogenes and S.
aureus (P < 0.05). The function of CFS on biogenic amine production
by foodborne pathogens varied depending on strains and specific
amine. Cadaverine formation by L. monocytogenes and S. aureus in
control were 500.9 and 948.1 mg/L, respectively while the CFSs of
LAB induced 4-fold lower cadaverine production by L.
monocytogenes and 7-fold lower cadaverine production by S. aureus.
The CFSs resulted in strong decreases in cadaverine and putrescine
production by L. monocytogenes and S. aureus, although remarkable
increases were observed for histamine, spermidine, spermine,
serotonin, dopamine, tyramine and agmatine in the presence of LAB
in lysine decarboxylase broth.
Abstract: The aim of this work is to build a model based on
tissue characterization that is able to discriminate pathological and
non-pathological regions from three-phasic CT images. With our
research and based on a feature selection in different phases, we are
trying to design a neural network system with an optimal neuron
number in a hidden layer. Our approach consists of three steps:
feature selection, feature reduction, and classification. For each
region of interest (ROI), 6 distinct sets of texture features are
extracted such as: first order histogram parameters, absolute gradient,
run-length matrix, co-occurrence matrix, autoregressive model, and
wavelet, for a total of 270 texture features. When analyzing more
phases, we show that the injection of liquid cause changes to the high
relevant features in each region. Our results demonstrate that for
detecting HCC tumor phase 3 is the best one in most of the features
that we apply to the classification algorithm. The percentage of
detection between pathology and healthy classes, according to our
method, relates to first order histogram parameters with accuracy of
85% in phase 1, 95% in phase 2, and 95% in phase 3.
Abstract: The effects of basil and/or chamomile seed
supplementation on the growth of Hubbard broiler chicks were
evaluated. The antioxidant effects of these supplements were also
assessed. 120 1-day-old broiler chicks were randomly divided into
four equal groups. The control group (group 1) was fed a basal diet
(BD) without supplementation. Groups 2, 3, and 4 were fed the BD
supplemented with 10g basil, 10g chamomile, and 5g basil plus 5g
chamomile per kg of food, respectively. Basil supplementation alone
or in combination with chamomile non-significantly (P≥0.05)
increased final body weight (3.2% and 0.3%, respectively) and
weight gain (3.5% and 3.6%, respectively) over the experimental
period. Chamomile supplementation alone non-significantly (P≥0.05)
reduced final body weight and weight gain over the experimental
period by 1.7% and 1.7%, respectively. In comparison to the control
group, herbal seed supplementation reduced feed intake and
improved the feed conversion and protein efficiency ratios. In
general, basil seed supplementation stimulated chicken growth and
improved the feed efficiency more effectively than chamomile seed
supplementation. The antioxidant activities of basil and/or chamomile
supplementation were examined in the thymus, bursa, and spleen. In
chickens that received supplements, the level of malondialdehyde
was significantly decreased, whereas the activities of glutathione,
superoxide dismutase, and catalase were significantly increased
(P
Abstract: Sound exposure effects have been investigated by
broadcasting a group of broilers with sound of Quran verses (Group
B) whereas the other group is the control broilers (Group C). The
growth rate comparisons in terms of weight and raw meat texture
measured by shear force have been investigated. Twenty-seven
broilers were randomly selected from each group on Day 24 and
weight measurement was carried out every week till the harvest day
(Day 39).Group B showed a higher mean weight on Day 24 (1.441 ±
0.013 kg) than Group C. Significant difference in the weight on Day
39 existed for Group B compared to Group C (p < 0.05). However,
there was no significant (p >0.05) difference of shear force in the
same muscles (breast and drumstick raw meat) of both groups but the
shear force of the breast meat for Group B and C broilers was lower
(p < 0.05) than that of their drumstick meat. Thus, broadcasting the
sound of Quran verses in the coop can be applied to improve the
growth rate of broilers for producing better quality poultry.
Abstract: Speech Segmentation is the measure of the change
point detection for partitioning an input speech signal into regions
each of which accords to only one speaker. In this paper, we apply
two features based on multi-scale product (MP) of the clean speech,
namely the spectral centroid of MP, and the zero crossings rate of
MP. We focus on multi-scale product analysis as an important tool
for segmentation extraction. The MP is based on making the product
of the speech wavelet transform coefficients (WTC). We have
estimated our method on the Keele database. The results show the
effectiveness of our method. It indicates that the two features can find
word boundaries, and extracted the segments of the clean speech.
Abstract: Currently, there is excessively growing information
about places on Facebook, which is the largest social network but
such information is not explicitly organized and ranked. Therefore
users cannot exploit such data to recommend places conveniently and
quickly. This paper proposes a Facebook application and an Android
application that recommend places based on the number of check-ins
of those places, the distance of those places from the current location,
the number of people who like Facebook page of those places, and
the number of talking about of those places. Related Facebook data is
gathered via Facebook API requests. The experimental results of the
developed applications show that the applications can recommend
places and rank interesting places from the most to the least. We have
found that the average satisfied score of the proposed Facebook
application is 4.8 out of 5. The users’ satisfaction can increase by
adding the app features that support personalization in terms of
interests and preferences.
Abstract: The western Tombolo of the Giens peninsula in
southern France, known as Almanarre beach, is subject to coastal
erosion. We are trying to use computer simulation in order to propose
solutions to stop this erosion. Our aim was first to determine the main
factors for this erosion and successfully apply a coupled hydrosedimentological
numerical model based on observations and
measurements that have been performed on the site for decades.
We have gathered all available information and data about waves,
winds, currents, tides, bathymetry, coastal line, and sediments
concerning the site. These have been divided into two sets: one
devoted to calibrating a numerical model using Mike 21 software, the
other to serve as a reference in order to numerically compare the
present situation to what it could be if we implemented different
types of underwater constructions.
This paper presents the first part of the study: selecting and
melting different sources into a coherent data basis, identifying the
main erosion factors, and calibrating the coupled software model
against the selected reference period.
Our results bring calibration of the numerical model with good
fitting coefficients. They also show that the winter South-Western
storm events conjugated to depressive weather conditions constitute a
major factor of erosion, mainly due to wave impact in the northern
part of the Almanarre beach. Together, current and wind impact is
shown negligible.
Abstract: The tombolo of Giens is located in the town of Hyères
(France). We recall the history of coastal erosion, and prominent
factors affecting the evolution of the western tombolo. We then
discuss the possibility of stabilizing the western tombolo. Our
argumentation relies on a coupled model integrating swells, currents,
water levels and sediment transport. We present the conclusions of
the simulations of various scenarios, including pre-existing
propositions from coastal engineering offices. We conclude that
beach replenishment seems to be necessary but not sufficient for the
stabilization of the beach. Breakwaters reveal effective particularly in
the most exposed northern area. Some solutions fulfill conditions so
as to be elected as satisfactory. We give a comparative analysis of the
efficiency of 14 alternatives for the protection of the tombolo.
Abstract: A new method for determining the distribution of
birefringence and linear dichroism in optical polymer materials is
presented. The method is based on the use of polarizationholographic
diffraction grating that forms an orthogonal circular basis
in the process of diffraction of probing laser beam on the grating. The
intensities ratio of the orders of diffraction on this grating enables the
value of birefringence and linear dichroism in the sample to be
determined. The distribution of birefringence in the sample is
determined by scanning with a circularly polarized beam with a
wavelength far from the absorption band of the material. If the
scanning is carried out by probing beam with the wavelength near to
a maximum of the absorption band of the chromophore then the
distribution of linear dichroism can be determined. An appropriate
theoretical model of this method is presented. A laboratory setup was
created for the proposed method. An optical scheme of the laboratory
setup is presented. The results of measurement in polymer films with
two-dimensional gradient distribution of birefringence and linear
dichroism are discussed.
Abstract: Mahasweta Devi and Toni Morrison are the two
stalwarts of the Indian English and the Afro-American literature
respectively. The writings of these two novelists are authentic and
powerful records of the lives of the people because much of their
personal experiences have gone into the making of their works. Devi,
a representative force of the Indian English literature, is also a social
activist working with the tribals of Bihar, Jharkhand, Orissa and West
Bengal. Most of her works echo the lives and struggles of the
subalterns as is evident in her “best beloved book” Chotti Munda and
His Arrow. The novelist focuses on the struggle of the tribals against
the colonial and the feudal powers to create their own identity,
thereby, embarking on the ideological project of ‘setting the record
straight’. The Nobel Laureate Toni Morrison, on the other hand,
brings to the fore the crucial issues of gender, race and class in many
of her significant works. In one of her representative works Sula, the
protagonist emerges as a non- conformist and directly confronts the
notion of a ‘good woman’ nurtured by the community of the Blacks.
In addition to this, the struggle of the Blacks against the White
domination, also become an important theme of the text. The thrust
of the paper lies in making a critical analysis of the portrayal of the
heroic attempts of the subaltern protagonist and the artistic endeavor
of the novelists in challenging the stereotypes.