Abstract: Adult mesenchymal stem cells (MSCs) have been investigated using preclinical approaches for tissue regeneration. Porcine MSCs (pMSCs) are capable of growing and attaching to plastic with a fibroblast-like morphology and then differentiating into bone, adipose, and cartilage tissues in vitro. This study was conducted to investigate the proliferating abilities, differentiation potentials, and multipotency of miniature pig adipose tissue-derived MSCs (mpAD-MSCs) with or without long-term cryopreservation, considering that cryostorage has the potential for use in clinical applications. After confirming the characteristics of the mpAD-MSCs, we examined the effect of long-term cryopreservation (> 2 years) on expression of cell surface markers (CD34, CD90 and CD105), proliferating abilities (cumulative population doubling level, doubling time, colony-forming unit, and MTT assay) and differentiation potentials into mesodermal cell lineages. As a result, the expression of cell surface markers is similar between thawed and fresh mpAD-MSCs. However, long-term cryopreservation significantly lowered the differentiation potentials (adipogenic, chondrogenic, and osteogenic) of mpAD-MSCs. When compared with fresh mpAD-MSCs, thawed mpAD-MSCs exhibited lower expression of mesodermal cell lineage-related genes such as peroxisome proliferator-activated receptor-g2, lipoprotein lipase, collagen Type II alpha 1, osteonectin, and osteocalcin. Interestingly, long-term cryostoraged mpAD-MSCs exhibited significantly higher cell viability than the fresh mpAD-MSCs. Long-term cryopreservation induced a 30% increase in the cell viability of mpAD-MSCs when compared with the fresh mpAD-MSCs at 5 days after thawing. However, long-term cryopreservation significantly lowered expression of stemness markers such as Oct3/4, Sox2, and Nanog. Furthermore, long-term cryopreservation negatively affected expression of senescence-associated genes such as telomerase reverse transcriptase and heat shock protein 90 of mpAD-MSCs when compared with the fresh mpAD-MSCs. The results from this study might be important for the successful application of MSCs in clinical trials after long-term cryopreservation.
Abstract: The objective of this research is to forecast the monthly exchange rate between Thai baht and the US dollar and to compare two forecasting methods. The methods are Box-Jenkins’ method and Holt’s method. Results show that the Box-Jenkins’ method is the most suitable method for the monthly Exchange Rate between Thai Baht and the US Dollar. The suitable forecasting model is ARIMA (1,1,0) without constant and the forecasting equation is Yt = Yt-1 + 0.3691 (Yt-1 - Yt-2) When Yt is the time series data at time t, respectively.
Abstract: The collapse of the infamous Rana Plaza, a multi-storeyed commercial building in Savar, near Dhaka, Bangladesh has brought with it a plethora of positive and negative consequences. Bangladesh being a key player in the export of clothing, found itself amidst a wave of economic upheaval following this tragic incident that resulted in numerous Bangladeshis, most of whom were factory workers. This paper compares the consequences that the country’s Ready Made Garments (RMG) sector is facing now, two years into the incident. The paper presents a comparison of statistical data from study reports and brings forward perspectives from all dimensions of Labour, Employment and Industrial Relations in Bangladesh following the event. The paper brings across the viewpoint of donor organizations and donor countries, the impacts of several initiatives taken by foreign organizations like the International Labour Organization, and local entities like the Bangladesh Garment Manufacturers and Exporters Association (BGMEA) in order to reinforce compliance and stabilize the shaky foundation that the RMG sector had found itself following the collapse. Focus of the paper remains on the stance taken by the suppliers in Bangladesh, with inputs from buying houses and factories, and also on the reaction of foreign brands. The paper also focuses on the horrific physical, mental and financial implications sustained by the victims and their families, and the consequent uproar from workers in general regarding compliance with work safety and workers’ welfare conditions. The purpose is to get across both sides of the scenario: the economic impact that suppliers / factories/ sellers/ buying houses/exporters have faced in Bangladesh as a result of complete loss of reliability on them regarding working standards; and also to cover the aftershock felt on the other end of the spectrum by the importers/ buyers, particularly the foreign entities, in terms of the sudden accountability of being affiliated with non- compliant factories. The collapse of Rana Plaza has received vast international attention and strong criticism. Nevertheless, the almost immediate strengthening of labourrights and the wholesale reform undertaken on all sides of the supply chain, evidence a move of all local and foreign stakeholders towards greater compliance and taking of precautionary steps for prevention of further disasters. The tragedy that Rana Plaza embodies served as a much-needed epiphany for the soaring RMG Sector of Bangladesh. Prompt co-operation on the part of all stakeholders and regulatory bodies now show a move towards sustainable development, which further ensures safeguarding against any future irregularities and pave the way for steady economic growth.
Abstract: In this paper, we present an analytical method for
analysis of nano-scale spherical shell subjected to thermo-mechanical
shocks based on nonlocal elasticity theory. Thermo-mechanical
properties of nano shpere is assumed to be temperature dependent.
Governing partial differential equation of motion is solved
analytically by using Laplace transform for time domain and power
series for spacial domain. The results in Laplace domain is
transferred to time domain by employing the fast inverse Laplace
transform (FLIT) method. Accuracy of present approach is assessed
by comparing the the numerical results with the results of published
work in literature. Furtheremore, the effects of non-local parameter
and wall thickness on the dynamic characteristics of the nano-sphere
are studied.
Abstract: 10 clinically healthy hemal nodes were collected from male bulls aged 2-3 years. Light microscopy revealed a capsule of connective tissue consisted mainly of collagen fiber surrounding hemal node, numerous erythrocytes were found in wide subcapsular sinus under the capsule. The parenchyma of the hemal node was divided into cortex and medulla. Diffused lymphocytes, and lymphoid follicles, having germinal centers were the main components of the cortex, while in the medulla there was wide medullary sinus, diffused lymphocytes and few lymphoid nodules. The area occupied with lymph nodules was larger than that occupied with non-nodular structure of lymphoid cords and blood sinusoids. Electron microscopy revealed the cellular components of hemal node including elements of circulating erythrocytes intermingled with lymphocytes, plasma cells, mast cells, reticular cells, macrophages, megakaryocytes and endothelial cells lining the blood sinuses. The lymphocytes were somewhat triangular in shape with cytoplasmic processes extending between adjacent erythrocytes. Nuclei were triangular to oval in shape, lightly stained with clear nuclear membrane indentation and clear nucleoli. The reticular cells were elongated in shape with cytoplasmic processes extending between adjacent lymphocytes, rough endoplasmic reticulum, ribosomes and few lysosomes were seen in their cytoplasm. Nucleus was elongated in shape with less condensed chromatin. Plasma cells were oval to irregular in shape with numerous dilated rough endoplasmic reticulum containing electron lucent material occupying the whole cytoplasm and few mitochondria were found. Nuclei were centrally located and oval in shape with heterochromatin emarginated and often clumped near the nuclear membrane. Occasionally megakaryocytes and mast cells were seen among lymphocytes. Megakaryocytes had multilobulated nucleus and free ribosomes often appearing as small aggregates in their cytoplasm, while mast cell had their characteristic electron dense granule in the cytoplasm, few electron lucent granules were found also, we conclude that, the main function of the hemal node of cattle is proliferation of lymphocytes. No role for plasma cell in erythrophagocytosis could be suggested.
Abstract: In order to understand whether there is a better than the
learning function of learning methods and improve the CAD Courses
for enterprise’s design human resource development, this research is
applied in learning practical learning computer graphics software. In
this study, Revit building information model for learning content,
design of two different modes of learning curriculum to learning,
learning functions, respectively, and project learning. Via a post-test,
questionnaires and student interviews, etc., to study the effectiveness
of a comparative analysis of two different modes of learning. Students
participate in a period of three weeks after a total of nine-hour course,
and finally written and hands-on test. In addition, fill in the
questionnaire response by the student learning, a total of fifteen
questionnaire title, problem type into the base operating software,
application software and software-based concept features three
directions. In addition to the questionnaire, and participants were
invited to two different learning methods to conduct interviews to
learn more about learning students the idea of two different modes.
The study found that the ad hoc short-term courses in learning, better
learning outcomes. On the other hand, functional style for the whole
course students are more satisfied, and the ad hoc style student is
difficult to accept the ad hoc style of learning.
Abstract: The maintenance of work rolls in hot strip processing
has been lengthy and difficult tasks for hot strip manufacturer
because heavy work rolls have to be taken out of the production line,
which could take hours. One way to increase the time between
maintenance is to improve the effectiveness of the work roll cooling
system such that the wear and tear more slowly occurs, while the
operation cost is kept low. Therefore, this study aims to improve the
work roll cooling system by providing the manufacturer the
relationship between the work-roll temperature reduced by cooling
and the water flow that can help manufacturer determining the more
effective water flow of the cooling system. The relationship is found
using simulation with a systematic process adjustment so that the
satisfying quality of product is achieved. Results suggest that the
manufacturer could reduce the water flow by 9% with roughly the
same performance. With the same process adjustment, the feasibility
of finishing-mill-stand reduction is also investigated. Results suggest
its possibility.
Abstract: The source of the jet noise is generated by rocket exhaust plume during rocket engine testing. A domain decomposition approach is applied to the jet noise prediction in this paper. The aerodynamic noise coupling is based on the splitting into acoustic sources generation and sound propagation in separate physical domains. Large Eddy Simulation (LES) is used to simulate the supersonic jet flow. Based on the simulation results of the flow-fields, the jet noise distribution of the sound pressure level is obtained by applying the Ffowcs Williams-Hawkings (FW-H) acoustics equation and Fourier transform. The calculation results show that the complex structures of expansion waves, compression waves and the turbulent boundary layer could occur due to the strong interaction between the gas jet and the ambient air. In addition, the jet core region, the shock cell and the sound pressure level of the gas jet increase with the nozzle size increasing. Importantly, the numerical simulation results of the far-field sound are in good agreement with the experimental measurements in directivity.
Abstract: This paper outlines the development of an
experimental technique in quantifying supersonic jet flows, in an
attempt to avoid seeding particle problems frequently associated with
particle-image velocimetry (PIV) techniques at high Mach numbers.
Based on optical flow algorithms, the idea behind the technique
involves using high speed cameras to capture Schlieren images of the
supersonic jet shear layers, before they are subjected to an adapted
optical flow algorithm based on the Horn-Schnuck method to
determine the associated flow fields. The proposed method is capable
of offering full-field unsteady flow information with potentially
higher accuracy and resolution than existing point-measurements or
PIV techniques. Preliminary study via numerical simulations of a
circular de Laval jet nozzle successfully reveals flow and shock
structures typically associated with supersonic jet flows, which serve
as useful data for subsequent validation of the optical flow based
experimental results. For experimental technique, a Z-type Schlieren
setup is proposed with supersonic jet operated in cold mode,
stagnation pressure of 4 bar and exit Mach of 1.5. High-speed singleframe
or double-frame cameras are used to capture successive
Schlieren images. As implementation of optical flow technique to
supersonic flows remains rare, the current focus revolves around
methodology validation through synthetic images. The results of
validation test offers valuable insight into how the optical flow
algorithm can be further improved to improve robustness and
accuracy. Despite these challenges however, this supersonic flow
measurement technique may potentially offer a simpler way to
identify and quantify the fine spatial structures within the shock shear
layer.
Abstract: Fermentation is well known as an essential process to
develop chocolate flavor in dried cocoa beans. Besides developing
the precursor of cocoa flavor, it also induces the color changes in the
beans. The fermentation process is influenced by various factors such
as planting material, preconditioning of cocoa pod and fermentation
technique. Therefore, this study was conducted to evaluate color of
Malaysian cocoa beans and how the duration of pods storage and
fermentation technique using shallow box will effect on its color
characteristics. There are two factors being studied i.e. duration of
cocoa pod storage (0, 2, 4 and 6 days) and duration of cocoa
fermentation (0, 1, 2, 3, 4 and 5 days). The experiment is arranged in
4 x 6 factorial designs with 24 treatments and arrangement is in a
Completely Randomised Design (CRD). The produced beans are
inspected for color changes under artificial light during cut test and
divided into four groups of color namely fully brown, purple brown,
fully purple and slaty. Cut tests indicated that cocoa beans which are
directly dried without undergone fermentation has the highest slaty
percentage. However, application of pods storage before fermentation
process is found to decrease the slaty percentage. In contrast, the
percentages of fully brown beans start to dominate after two days of
fermentation, especially from four and six days of pods storage batch.
Whereas, almost all batches of cocoa beans have a percentage of fully
purple less than 20%. Interestingly, the percentage of purple brown
beans are scattered in the entire beans batch regardless any specific
trend. Meanwhile, statistical analysis using General Linear Model
showed that the pods storage has a significant effect on the color
characteristic of the Malaysian dried beans compared to fermentation
duration.
Abstract: Small-size and low-power sensors with sensing, signal
processing and wireless communication capabilities is suitable for the
wireless sensor networks. Due to the limited resources and battery
constraints, complex routing algorithms used for the ad-hoc networks
cannot be employed in sensor networks. In this paper, we propose
node-disjoint multi-path hexagon-based routing algorithms in wireless
sensor networks. We suggest the details of the algorithm and compare
it with other works. Simulation results show that the proposed scheme
achieves better performance in terms of efficiency and message
delivery ratio.
Abstract: Opportunistic Routing (OR) increases the
transmission reliability and network throughput. Traditional routing
protocols preselects one or more predetermined nodes before
transmission starts and uses a predetermined neighbor to forward a
packet in each hop. The opportunistic routing overcomes the
drawback of unreliable wireless transmission by broadcasting one
transmission can be overheard by manifold neighbors. The first
cooperation-optimal protocol for Multirate OR (COMO) used to
achieve social efficiency and prevent the selfish behavior of the
nodes. The novel link-correlation-aware OR improves the
performance by exploiting the miscellaneous low correlated forward
links. Context aware Adaptive OR (CAOR) uses active suppression
mechanism to reduce packet duplication. The Context-aware OR
(COR) can provide efficient routing in mobile networks. By using
Cooperative Opportunistic Routing in Mobile Ad hoc Networks
(CORMAN), the problem of opportunistic data transfer can be
tackled. While comparing to all the protocols, COMO is the best as it
achieves social efficiency and prevents the selfish behavior of the
nodes.
Abstract: Escherichia coli (E. coli) is the most isolated bacteria
from blood circulation of septicemic calves. Given the prevalence of
septicemia in animals and its economic importance in veterinary
practice, better understanding of changes in clinical signs following
disease, may contribute to early detection of disorder. The present
study has been carried out to detect changes of clinical signs in
induced sepsis in calves with E. coli. Colisepticemia has been
induced in 10 twenty-day old healthy Holstein- Frisian calves with
intravenous injection of 1.5 X 109 colony forming units (cfu) of
O111:H8 strain of E. coli. Clinical signs including rectal temperature,
heart rate, respiratory rate, shock, appetite, sucking reflex, feces
consistency, general behavior, dehydration and standing ability were
recorded in experimental calves during 24 hours after induction of
colisepticemia. Blood culture was also carried out from calves four
times during experiment. ANOVA with repeated measure is used to
see changes of calves’ clinical signs to experimental colisepticemia,
and values of P≤ 0.05 was considered statistically significant. Mean
values of rectal temperature and heart rate as well as median values
of respiratory rate, appetite, suckling reflex, standing ability and feces
consistency of experimental calves increased significantly during
study (P 0.05). The
results of present study showed that total score of clinical signs in
calves with experimental colisepticemia increased significantly,
although score of some clinical signs such as shock did not change
significantly.
Abstract: The aim of this paper is to propose a general
framework for storing, analyzing, and extracting knowledge from
two-dimensional echocardiographic images, color Doppler images,
non-medical images, and general data sets. A number of high
performance data mining algorithms have been used to carry out this
task. Our framework encompasses four layers namely physical
storage, object identification, knowledge discovery, user level.
Techniques such as active contour model to identify the cardiac
chambers, pixel classification to segment the color Doppler echo
image, universal model for image retrieval, Bayesian method for
classification, parallel algorithms for image segmentation, etc., were
employed. Using the feature vector database that have been
efficiently constructed, one can perform various data mining tasks
like clustering, classification, etc. with efficient algorithms along
with image mining given a query image. All these facilities are
included in the framework that is supported by state-of-the-art user
interface (UI). The algorithms were tested with actual patient data
and Coral image database and the results show that their performance
is better than the results reported already.
Abstract: Routing in adhoc networks is a challenge as nodes are
mobile, and links are constantly created and broken. Present ondemand
adhoc routing algorithms initiate route discovery after a path
breaks, incurring significant cost to detect disconnection and
establish a new route. Specifically, when a path is about to be broken,
the source is warned of the likelihood of a disconnection. The source
then initiates path discovery early, avoiding disconnection totally. A
path is considered about to break when link availability decreases.
This study modifies Adhoc On-demand Multipath Distance Vector
routing (AOMDV) so that route handoff occurs through link
availability estimation.
Abstract: In this article, we used the residual correction method
to deal with transient thermoelastic problems with a hollow spherical
region when the continuum medium possesses spherically isotropic
thermoelastic properties. Based on linear thermoelastic theory, the
equations of hyperbolic heat conduction and thermoelastic motion
were combined to establish the thermoelastic dynamic model with
consideration of the deformation acceleration effect and non-Fourier
effect under the condition of transient thermal shock. The approximate
solutions of temperature and displacement distributions are obtained
using the residual correction method based on the maximum principle
in combination with the finite difference method, making it easier and
faster to obtain upper and lower approximations of exact solutions.
The proposed method is found to be an effective numerical method
with satisfactory accuracy. Moreover, the result shows that the effect
of transient thermal shock induced by deformation acceleration is
enhanced by non-Fourier heat conduction with increased peak stress.
The influence on the stress increases with the thermal relaxation time.
Abstract: During machining process, chatter is an unavoidable
phenomenon. Boring bars possess the cantilever shape and due to
this, it is subjected to chatter. The adverse effect of chatter includes
the increase in temperature which will leads to excess tool wear. To
overcome these problems, in this investigation, Cartridge brass (Cu –
70% and Zn – 30%) is passively fixed on the boring bar and also
clearance is provided in order to reduce the displacement, tool wear
and cutting temperature. A conventional all geared lathe is attached
with vibrometer and pyrometer is used to measure the displacement
and temperature. The influence of input parameters such as cutting
speed, depth of cut and clearance on temperature, tool wear and
displacement are investigated for various cutting conditions. From
the result, the optimum conditions to obtain better damping in boring
process for chatter reduction is identified.
Abstract: The development of composite materials and the
related design and manufacturing technologies is one of the most
important advances in the history of materials. Composites are
multifunctional materials having unprecedented mechanical and
physical properties that can be tailored to meet the requirements of a
particular application. Some composites also exhibit great resistance
to high-temperature corrosion, oxidation, and wear. Polymers are
widely used indoors and outdoors, therefore they are exposed to a
chemical environment which may include atmospheric oxygen, acidic
fumes, acidic rain, moisture heat and thermal shock, ultra-violet light,
high energy radiation, etc. Different polymers are affected differently
by these factors even though the amorphous polymers are more
sensitive. Ageing is also important and it is defined as the process of
deterioration of engineering materials resulting from the combined
effects of atmospheric radiation, heat, oxygen, water, microorganisms
and other atmospheric factors.
Abstract: The main issue in designing a wireless sensor network
(WSN) is the finding of a proper routing protocol that complies with
the several requirements of high reliability, short latency, scalability,
low power consumption, and many others. This paper proposes a
novel routing algorithm that complies with these design
requirements. The new routing protocol divides the WSN into several subnetworks
and each sub-network is divided into several clusters. This
division is designed to reduce the number of radio transmission and
hence decreases the power consumption. The network division may
be changed dynamically to adapt with the network changes and
allows the realization of the design requirements.
Abstract: A Mobile Adhoc Network (MANET) is a collection of mobile nodes that communicate with each other with wireless links and without pre-existing communication infrastructure. Routing is an important issue which impacts network performance. As MANETs lack central administration and prior organization, their security concerns are different from those of conventional networks. Wireless links make MANETs susceptible to attacks. This study proposes a new trust mechanism to mitigate wormhole attack in MANETs. Different optimization techniques find available optimal path from source to destination. This study extends trust and reputation to an improved link quality and channel utilization based Adhoc Ondemand Multipath Distance Vector (AOMDV). Differential Evolution (DE) is used for optimization.