Process Optimization for Enhanced Production of Cell Biomass and Metabolites of Fluorescent Pseudomonad R81

The fluorescent pseudomonad strain R81 is a root colonizing rhizobacteria which promotes the growth of many plants by various mechanisms. Its broth containing siderophore (ironchelating compound) and 2,4- diacetyl phloroglucinol (DAPG) is used for preparing bioinoculant formulations for agronomical applications. Glycerol was found to be the best carbon source for improved biomass production. Splitting of nitrogen source to NH4Cl and urea had a stabilizing effect on pH during batch cultivation. Ltryptophan at 0.5 % in the medium increased the siderophore production to 850 mg/l. During batch cultivation of the strain in a bioreactor, a maximum of 4 g/l of dry cell mass, 1.8 g/l of siderophore and 20 mg/l of DAPG was achieved when glycerol was 15 g/l and C/N ratio was maintained at 12.5. In case of intermittent feeding of fresh medium during fed-batch cultivation, the dry cell mass was increased to 25 g/l with improved production of DAPG to 70 mg/l.

Blind Identification of MA Models Using Cumulants

In this paper, many techniques for blind identification of moving average (MA) process are presented. These methods utilize third- and fourth-order cumulants of the noisy observations of the system output. The system is driven by an independent and identically distributed (i.i.d) non-Gaussian sequence that is not observed. Two nonlinear optimization algorithms, namely the Gradient Descent and the Gauss-Newton algorithms are exposed. An algorithm based on the joint-diagonalization of the fourth-order cumulant matrices (FOSI) is also considered, as well as an improved version of the classical C(q, 0, k) algorithm based on the choice of the Best 1-D Slice of fourth-order cumulants. To illustrate the effectiveness of our methods, various simulation examples are presented.

An Edge-based Text Region Extraction Algorithm for Indoor Mobile Robot Navigation

Using bottom-up image processing algorithms to predict human eye fixations and extract the relevant embedded information in images has been widely applied in the design of active machine vision systems. Scene text is an important feature to be extracted, especially in vision-based mobile robot navigation as many potential landmarks such as nameplates and information signs contain text. This paper proposes an edge-based text region extraction algorithm, which is robust with respect to font sizes, styles, color/intensity, orientations, and effects of illumination, reflections, shadows, perspective distortion, and the complexity of image backgrounds. Performance of the proposed algorithm is compared against a number of widely used text localization algorithms and the results show that this method can quickly and effectively localize and extract text regions from real scenes and can be used in mobile robot navigation under an indoor environment to detect text based landmarks.

Simulation Study for Performance Comparison of Routing Protocols in Mobile Adhoc Network

Due to insufficient frequency band and tremendous growth of the mobile users, complex computation is needed for the use of resources. Long distance communication began with the introduction of telegraphs and simple coded pulses, which were used to transmit short messages. Since then numerous advances have rendered reliable transfer of information both easier and quicker. Wireless network refers to any type of computer network that is wireless, and is commonly associated with a telecommunications network whose interconnections between nodes is implemented without the use of wires. Wireless network can be broadly categorized in infrastructure network and infrastructure less network. Infrastructure network is one in which we have a base station to serve the mobile users and in the infrastructure less network is one in which no infrastructure is available to serve the mobile users this kind of networks are also known as mobile Adhoc networks. In this paper we have simulated the result for different scenarios with protocols like AODV and DSR; we simulated the result for throughput, delay and receiving traffic in the given scenario.

Collaborative Car Pooling System

This paper describes the architecture for a collaborative Car Pooling System based on a credits mechanism to motivate the cooperation among users. Users can spend the accumulated credits on parking facilities. For this, we propose a business model to support the collaboration between a car pooling system and parking facilities. The Portuguese Lisbon-s Metropolitan area is used as application scenario.

Capacity Optimization for Local and Cooperative Spectrum Sensing in Cognitive Radio Networks

The dynamic spectrum allocation solutions such as cognitive radio networks have been proposed as a key technology to exploit the frequency segments that are spectrally underutilized. Cognitive radio users work as secondary users who need to constantly and rapidly sense the presence of primary users or licensees to utilize their frequency bands if they are inactive. Short sensing cycles should be run by the secondary users to achieve higher throughput rates as well as to provide low level of interference to the primary users by immediately vacating their channels once they have been detected. In this paper, the throughput-sensing time relationship in local and cooperative spectrum sensing has been investigated under two distinct scenarios, namely, constant primary user protection (CPUP) and constant secondary user spectrum usability (CSUSU) scenarios. The simulation results show that the design of sensing slot duration is very critical and depends on the number of cooperating users under CPUP scenario whereas under CSUSU, cooperating more users has no effect if the sensing time used exceeds 5% of the total frame duration.

Low Resolution Single Neural Network Based Face Recognition

This research paper deals with the implementation of face recognition using neural network (recognition classifier) on low-resolution images. The proposed system contains two parts, preprocessing and face classification. The preprocessing part converts original images into blurry image using average filter and equalizes the histogram of those image (lighting normalization). The bi-cubic interpolation function is applied onto equalized image to get resized image. The resized image is actually low-resolution image providing faster processing for training and testing. The preprocessed image becomes the input to neural network classifier, which uses back-propagation algorithm to recognize the familiar faces. The crux of proposed algorithm is its beauty to use single neural network as classifier, which produces straightforward approach towards face recognition. The single neural network consists of three layers with Log sigmoid, Hyperbolic tangent sigmoid and Linear transfer function respectively. The training function, which is incorporated in our work, is Gradient descent with momentum (adaptive learning rate) back propagation. The proposed algorithm was trained on ORL (Olivetti Research Laboratory) database with 5 training images. The empirical results provide the accuracy of 94.50%, 93.00% and 90.25% for 20, 30 and 40 subjects respectively, with time delay of 0.0934 sec per image.

Synthesis and Fluorescence Spectroscopy of Sulphonic Acid-Doped Polyaniline When Exposed to Oxygen Gas

Three sulphonic acid-doped polyanilines were synthesized through chemical oxidation at low temperature (0-5 oC) and potential of these polymers as sensing agent for O2 gas detection in terms of fluorescence quenching was studied. Sulphuric acid, dodecylbenzene sulphonic acid (DBSA) and camphor sulphonic acid (CSA) were used as doping agents. All polymers obtained were dark green powder. Polymers obtained were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible absorption spectroscopy, thermogravimetry analysis, elemental analysis, differential scanning calorimeter and gel permeation chromatography. Characterizations carried out showed that polymers were successfully synthesized with mass recovery for sulphuric aciddoped polyaniline (SPAN), DBSA-doped polyaniline (DBSA-doped PANI) and CSA-doped polyaniline (CSA-doped PANI) of 71.40%, 75.00% and 39.96%, respectively. Doping level of SPAN, DBSAdoped PANI and CSA-doped PANI were 32.86%, 33.13% and 53.96%, respectively as determined based on elemental analysis. Sensing test was carried out on polymer sample in the form of solution and film by using fluorescence spectrophotometer. Samples of polymer solution and polymer film showed positive response towards O2 exposure. All polymer solutions and films were fully regenerated by using N2 gas within 1 hour period. Photostability study showed that all samples of polymer solutions and films were stable towards light when continuously exposed to xenon lamp for 9 hours. The relative standard deviation (RSD) values for SPAN solution, DBSA-doped PANI solution and CSA-doped PANI solution for repeatability were 0.23%, 0.64% and 0.76%, respectively. Meanwhile RSD values for reproducibility were 2.36%, 6.98% and 1.27%, respectively. Results for SPAN film, DBSAdoped PANI film and CSA-doped PANI film showed the same pattern with RSD values for repeatability of 0.52%, 4.05% and 0.90%, respectively. Meanwhile RSD values for reproducibility were 2.91%, 10.05% and 7.42%, respectively. The study on effect of the flow rate on response time was carried out using 3 different rates which were 0.25 mL/s, 1.00 mL/s and 2.00 mL/s. Results obtained showed that the higher the flow rate, the shorter the response time.

Simulation of a Sustainable Cement Supply Chain; Proposal Model Review

In recent years, sustainable supply chain management (SSCM) has been widely researched in academic domain. However, due to the traditional operational role and the complexity of supply chain management in the cement industry, a relatively small amount of research has been conducted on cement supply chain simulation integrated with sustainability criteria. This paper analyses the cement supply chain operations using the Push-Pull supply chain frameworks, the Life Cycle Assessment (LCA) methodology; and proposal integration approach, proposes three supply chain scenarios based on Make-To-Stock (MTS), Pack-To-Order (PTO) and Grind- To-Order (GTO) strategies. A Discrete-Event Simulation (DES) model of SSCM is constructed using Arena software to implement the three-target scenarios. We conclude with the simulation results that (GTO) is the optimal supply chain strategy that demonstrates the best economic, ecological and social performance in the cement industry.

Optimal Placement of Processors based on Effective Communication Load

This paper presents a new technique for the optimum placement of processors to minimize the total effective communication load under multi-processor communication dominated environment. This is achieved by placing heavily loaded processors near each other and lightly loaded ones far away from one another in the physical grid locations. The results are mathematically proved for the Algorithms are described.

The Role of Driving Experience in Hazard Perception and Categorization: A Traffic-Scene Paradigm

This study examined the role of driving experience in hazard perception and categorization using traffic scene pictures. Specifically, young-inexperienced, moderately experienced and very experienced (taxi) drivers observed traffic scene pictures while connected to an eye tracking system and were asked to rate the level of hazardousness of each picture and to mention the three most prominent hazards in it. Target pictures included nine, nearly identical, pairs of pictures where one picture in each pair included an actual hazard as an additional element. Altogether, 22 areas of interest (AOIs) were predefined and included 13 potential hazards and 9 actual hazards. Data analysis included both verbal reports and eye scanning patterns of these AOIs. Generally, both experienced and taxi drivers noted a relatively larger number of potential hazards than young inexperienced drivers Thus, by relating to less salient potential hazards, experienced drivers have demonstrated a better situation model of the traffic environment.

Miocene Warm Tropical Climate: Evidence Based on Oxygen Isotope in Central Java, Indonesia

Oxygen and carbon isotopes records of multi-species planktonic, benthic foraminifera and bulk carbonate sample from Central Java Indonesia demonstrate that warm sea surface temperature occurred during the Miocene. Planktonic δ18O values from this study consistently lighter (-4 to -3 ‰PDB) than previous studies that indicate sea surface temperature during Miocene in this area was warm than tropical/equatorial localities. A surprising decrease of oxygen isotopic composition was recorded at ±14 Ma where the maximum of δ18O values is -4.87 ‰PDB for Orbulina universa, -5.02 ‰PDB for Globigerinoides sacculifer and -4.30 ‰PDB for Globoquadrina dehiscens, this event we predict as Middle Miocene Optimum. Warming of sea surface temperature we interpret as related to the development of Western Pacific Warm Pool where warm water from Pacific Ocean through the Indonesian seaway appears to remain during Miocene. Our result also show increasing suddenly of oxygen isotope values of planktic, benthic and bulk carbonate sample from ± 12 Ma, the increasing cooled surface water relatively high degree with Late Miocene global cooling climate or we predict that due to closing of Indonesian Gateway.

Comparative Analysis of Various Multiuser Detection Techniques in SDMA-OFDM System Over the Correlated MIMO Channel Model for IEEE 802.16n

SDMA (Space-Division Multiple Access) is a MIMO (Multiple-Input and Multiple-Output) based wireless communication network architecture which has the potential to significantly increase the spectral efficiency and the system performance. The maximum likelihood (ML) detection provides the optimal performance, but its complexity increases exponentially with the constellation size of modulation and number of users. The QR decomposition (QRD) MUD can be a substitute to ML detection due its low complexity and near optimal performance. The minimum mean-squared-error (MMSE) multiuser detection (MUD) minimises the mean square error (MSE), which may not give guarantee that the BER of the system is also minimum. But the minimum bit error rate (MBER) MUD performs better than the classic MMSE MUD in term of minimum probability of error by directly minimising the BER cost function. Also the MBER MUD is able to support more users than the number of receiving antennas, whereas the rest of MUDs fail in this scenario. In this paper the performance of various MUD techniques is verified for the correlated MIMO channel models based on IEEE 802.16n standard.

Towards a Sustained Use of Renewable Energy Sources in Romania

The paper presents the potential for RES in Romania and the results of the Romanian national research project “Romania contribution to the European targets regarding the development of renewable energy sources - PROMES". The objective of the project is the development of energy generation from renewable energy sources (RES) in Romania by drawing up scenarios and prognosis harmonized with national and European targets, RES development effects modeling (environmental, economic, social etc.), research of the impact of the penetration of RES into the main, implementation of an advanced software system tool for RES information recording and communication, experimental research based on demonstrative applications. The expected results are briefly presented, as well as the social, economic and environmental impact.

An Environmental Impact Tool to Assess National Energy Scenarios

The Long-range Energy and Alternatives Planning (LEAP) energy planning system has been developed for South Africa, for the 2005 base year and a limited number of plausible future scenarios that may have significant implications (negative or positive) in terms of environmental impacts. The system quantifies the national energy demand for the domestic, commercial, transport, industry and agriculture sectors, the supply of electricity and liquid fuels, and the resulting emissions. The South African National Energy Research Institute (SANERI) identified the need to develop an environmental assessment tool, based on the LEAP energy planning system, to provide decision-makers and stakeholders with the necessary understanding of the environmental impacts associated with different energy scenarios. A comprehensive analysis of indicators that are used internationally and in South Africa was done and the available data was accessed to select a reasonable number of indicators that could be utilized in energy planning. A consultative process was followed to determine the needs of different stakeholders on the required indicators and also the most suitable form of reporting. This paper demonstrates the application of Energy Environmental Sustainability Indicators (EESIs) as part of the developed tool, which assists with the identification of the environmental consequences of energy generation and use scenarios and thereby promotes sustainability, since environmental considerations can then be integrated into the preparation and adoption of policies, plans, programs and projects. Recommendations are made to refine the tool further for South Africa.

Application of Fluorescent Pseudomonads Inoculant Formulations on Vigna mungo through Field Trial

Vermiculite was used to develop inorganic carrier-based formulations of fluorescent pseudomonad strains R62 and R81. The effect of bio-inoculation of fluorescent pseudomonad strains R62 and R81 (plant growth promoting and biocontrol agent) on growth responses of Vigna-mungo under field condition was enumerated. The combined bioinoculation of these two organisms in a formuation increased the pods yield by 300% in comparison to the control crop. There was also significant increment in the other plant growth responses such as dry root weight, dry shoot weight, shoot length and number of branches per plant.

Contrast-Enhanced Multispectal Upconversion Fluorescence Analysis for High-Resolution in-vivo Deep Tissue Imaging

Lanthanide-doped upconversion nanoparticles which can convert near-infrared lights to visible lights have attracted growing interest because of their great potentials in fluorescence imaging. Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. However, improving the detection sensitivity and decreasing the absorption and scattering in biological tissues are as yet unresolved problems. In this present study, a novel NIR-reflected multispectral imaging system was developed for upconversion fluorescent imaging in small animals. Based on this system, we have obtained the high contrast images without the autofluorescence when biocompatible UCPs were injected near the body surface or deeply into the tissue. Furthermore, we have extracted respective spectra of the upconversion fluorescence and relatively quantify the fluorescence intensity with the multispectral analysis. To our knowledge, this is the first time to analyze and quantify the upconversion fluorescence in the small animal imaging.

Virtual Learning Process Environment: Cohort Analytics for Learning and Learning Processes

Traditional higher-education classrooms allow lecturers to observe students- behaviours and responses to a particular pedagogy during learning in a way that can influence changes to the pedagogical approach. Within current e-learning systems it is difficult to perform continuous analysis of the cohort-s behavioural tendency, making real-time pedagogical decisions difficult. This paper presents a Virtual Learning Process Environment (VLPE) based on the Business Process Management (BPM) conceptual framework. Within the VLPE, course designers can model various education pedagogies in the form of learning process workflows using an intuitive flow diagram interface. These diagrams are used to visually track the learning progresses of a cohort of students. This helps assess the effectiveness of the chosen pedagogy, providing the information required to improve course design. A case scenario of a cohort of students is presented and quantitative statistical analysis of their learning process performance is gathered and displayed in realtime using dashboards.

Analysis of Plasmids and Restriction Fragment Length Polymorphisms of Acinetobacter baumannii Isolated from Hospitals- AL Jouf Region- KSA

Abstract–The objectives of the current study are to determine the prevalence, etiological agents, drug susceptibility pattern and plasmid profile of Acinetobacter baumannii isolates from Hospital-Acquired Infections (HAI) at Community Hospital, Al Jouf Province, Saudi Arabia. A total of 1890 patients had developed infection during hospital admission and were included in the study. Among those who developed nosocomial infections, 15(9.4), 10(2.7) and 118 (12.7) had respiratory tract infection (RTI), blood stream infections (BSI) and urinary tract (UTI) respectively. A total of 268 bacterial isolates were isolated from nosocomial infection. S. aureus was reported in 23.5% for of the total isolates followed by Klebsiella pneumoniae (17.5%), E. coli (17.2%), P. aeruginosa (11.9%), coagulase negative staphylococcus (9%), A. baumannii (7.1%), Enterobacter spp. (3.4%), Citrobacter freundii (3%), Proteus mirabilis (2.6%), and Proteus vulgaris and Enterococcous faecalis (0.7%). Isolated organisms are multi-drug resistant, predominantly Gram-positive pathogens with a high incidence of methicillin-resistant S. aureus, extended spectrum beta lactamase and vancomycin resistant enterococci organisms. The RFLP (Fragment Length Polymorphisms) patterns of plasmid preparations from isolated A. baumannii isolates had altered RFLP patterns, possibly due to the presence of plasmid(s). Five A. baumannii isolates harbored plasmids all of which were not less than 2.71kbp in molecular weight. Hence, it showed that the gene coding for the isolates were located on the plasmid DNA while the remaining isolates which have no plasmid might showed gene coding for antibiotic resistance being located on chromosomal DNA. Nosocomial infections represent a current problem in Community Hospital, Al Jouf Province, Saudi Arabia. Problems associated with SSI include infection with multidrug resistant pathogens which are difficult to treat and are associated with increased mortality.

Effects of Wastewater Strength and Salt Stress on Microalgal Biomass Production and Lipid Accumulation

This work aims to investigate a potential of microalgae for utilizing industrial wastewater as a cheap nutrient for their growth and oil accumulation. Wastewater was collected from the effluent ponds of agro-industrial factories (cassava and ethanol production plants). Only 2 microalgal strains were isolated and identified as Scenedesmus quadricauda and Chlorella sp.. However, only S. quadricauda was selected to cultivate in various wastewater concentrations (10%, 20%, 40%, 60%, 80% and 100%). The highest biomass obtained at 6.6×106 and 6.27×106 cells/ml when 60% wastewater was used in flask and photo-bioreactor. The cultures gave the highest lipid content at 18.58 % and 42.86% in cases of S. quadricauda and S. obliquus. In addition, under salt stress (1.0 M NaCl), S. obliquus demonstrated the highest lipid content at 50% which was much more than the case of no NaCl adding. However, the concentration of NaCl does not affect on lipid accumulation in case of S. quadricauda.