Avicelase Production by a Thermophilic Geobacillus stearothermophilus Isolated from Soil using Sugarcane Bagasse

Studies were carried out on the comparative study of the production of Avicelase enzyme using sugarcane bagasse-SCB in two different statuses (i.e. treated and untreated SCB) by thermophilic Geobacillus stearothermophilus at 50ºC. Only four thermophilic bacterial isolates were isolated and assayed for Avicelase production using UntSCB and TSCB. Only one isolate selected as most potent and identified as G. stearothermophilus used in this study. A specific endo-β-1,4-D-glucanase (Avicelase EC 3.2.1.91) was partially purified from a thermophilic bacterial strain was isolated from different soil samples when grown on cellulose enrichment SCB substrate as the sole carbon source. Results shown that G. stearothermophilus was the better Avicelase producer strain. Avicelase had an optimum pH and temperature 7.0 and 50ºC for both UntSCB and TSCB and exhibited good pH stability between "5-8" and "4-9", however, good temperature stability between (30-80ºC) for UntSCB and TSCB, respectively. Other factors affecting the production of Avicelase were compared (i.e. SCB concentration, inoculum size and different incubation periods), all results observed and obtained were revealed that the TSCB was exhibited maximal enzyme activity in comparison with the results obtained from UntSCB, so, the TSCB was enhancing the Avicelase production.

Scientific Orientation of Youth as the Basis of Formation of a New University Culture

At present the process of formation of corporate values in Kazakh universities is under the influence of a whole range of socio-economic and cultural changes: on the one hand universities must maintain and transmit traditional cultural values of education, on the other, to improve quality of service and to involve young people to science, providing thus own competitiveness. Thus, this article presents some results of two cycles of sociological research conducted in 2012 and aimed at identifying possible ways to popularize science and readiness to participate of youth in given activities, expectations of young scientists and the prospects of future development of the Kazakh science.

Stochastic Subspace Modelling of Turbulence

Turbulence of the incoming wind field is of paramount importance to the dynamic response of civil engineering structures. Hence reliable stochastic models of the turbulence should be available from which time series can be generated for dynamic response and structural safety analysis. In the paper an empirical cross spectral density function for the along-wind turbulence component over the wind field area is taken as the starting point. The spectrum is spatially discretized in terms of a Hermitian cross-spectral density matrix for the turbulence state vector which turns out not to be positive definite. Since the succeeding state space and ARMA modelling of the turbulence rely on the positive definiteness of the cross-spectral density matrix, the problem with the non-positive definiteness of such matrices is at first addressed and suitable treatments regarding it are proposed. From the adjusted positive definite cross-spectral density matrix a frequency response matrix is constructed which determines the turbulence vector as a linear filtration of Gaussian white noise. Finally, an accurate state space modelling method is proposed which allows selection of an appropriate model order, and estimation of a state space model for the vector turbulence process incorporating its phase spectrum in one stage, and its results are compared with a conventional ARMA modelling method.

Identification Common Microbes Observed on Polyester Tufting

Tufting carpet is a very suitable substrate for growing microorganism such as pathogenic microbes, due to the direct touch with human body, long washing periods and laying on the floor; in fact there are 3 major problems: To risk human health, Prepare bad odors and Destruction of the products.. In the presented research, for investigation of presence most common microbes on polyester tufting, first goods laid in a public place (in the corridor fair) for 30 days and the existence of some microbes were investigate on it with two methods of enrichment in nutrient environments such as thioglycolate and noutrunt brath, and shake the dust off the polyester tufting onto cultivation mediums such as blood agar and noutrunt agar. After the microorganism colonics are grown, the colonies were separated and six microbial tests such as cataloes and sitrat were carried out in five phases on the colonics for identifying the varieties of bacteria. As a result of tests, 5 type of bacteria, such as Escherichia coli, staphylococcus saprophytic as were identified. Each of the mentioned bacteria can be seriously harmful for the heath of human.

A Prototype of Augmented Reality for Visualising Large Sensors’ Datasets

In this paper we discuss the development of an Augmented Reality (AR) - based scientific visualization system prototype that supports identification, localisation, and 3D visualisation of oil leakages sensors datasets. Sensors generates significant amount of multivariate datasets during normal and leak situations. Therefore we have developed a data model to effectively manage such data and enhance the computational support needed for the effective data explorations. A challenge of this approach is to reduce the data inefficiency powered by the disparate, repeated, inconsistent and missing attributes of most available sensors datasets. To handle this challenge, this paper aim to develop an AR-based scientific visualization interface which automatically identifies, localise and visualizes all necessary data relevant to a particularly selected region of interest (ROI) along the virtual pipeline network. Necessary system architectural supports needed as well as the interface requirements for such visualizations are also discussed in this paper.

Haptics Enabled Offline AFM Image Analysis

Current advancements in nanotechnology are dependent on the capabilities that can enable nano-scientists to extend their eyes and hands into the nano-world. For this purpose, a haptics (devices capable of recreating tactile or force sensations) based system for AFM (Atomic Force Microscope) is proposed. The system enables the nano-scientists to touch and feel the sample surfaces, viewed through AFM, in order to provide them with better understanding of the physical properties of the surface, such as roughness, stiffness and shape of molecular architecture. At this stage, the proposed work uses of ine images produced using AFM and perform image analysis to create virtual surfaces suitable for haptics force analysis. The research work is in the process of extension from of ine to online process where interaction will be done directly on the material surface for realistic analysis.

Statistical Optimization of the Enzymatic Saccharification of the Oil Palm Empty Fruit Bunches

A statistical optimization of the saccharification process of EFB was studied. The statistical analysis was done by applying faced centered central composite design (FCCCD) under response surface methodology (RSM). In this investigation, EFB dose, enzyme dose and saccharification period was examined, and the maximum 53.45% (w/w) yield of reducing sugar was found with 4% (w/v) of EFB, 10% (v/v) of enzyme after 120 hours of incubation. It can be calculated that the conversion rate of cellulose content of the substrate is more than 75% (w/w) which can be considered as a remarkable achievement. All the variables, linear, quadratic and interaction coefficient, were found to be highly significant, other than two coefficients, one quadratic and another interaction coefficient. The coefficient of determination (R2) is 0.9898 that confirms a satisfactory data and indicated that approximately 98.98% of the variability in the dependent variable, saccharification of EFB, could be explained by this model.

Positive Periodic Solutions in a Discrete Competitive System with the Effect of Toxic Substances

In this paper, a delayed competitive system with the effect of toxic substances is investigated. With the aid of differential equations with piecewise constant arguments, a discrete analogue of continuous non-autonomous delayed competitive system with the effect of toxic substances is proposed. By using Gaines and Mawhin,s continuation theorem of coincidence degree theory, a easily verifiable sufficient condition for the existence of positive solutions of difference equations is obtained.

Effect of Concrete Nonlinear Parameters on the Seismic Response of Concrete Gravity Dams

Behavior of dams against the seismic loads has been studied by many researchers. Most of them proposed new numerical methods to investigate the dam safety. In this paper, to study the effect of nonlinear parameters of concrete in gravity dams, a twodimensional approach was used including the finite element method, staggered method and smeared crack approach. Effective parameters in the models are physical properties of concrete such as modulus of elasticity, tensile strength and specific fracture energy. Two different models were used in foundation (mass-less and massed) in order to determine the seismic response of concrete gravity dams. Results show that when the nonlinear analysis includes the dam- foundation interaction, the foundation-s mass, flexibility and radiation damping are important in gravity dam-s response.

Evolutionary Origin of the αC Helix in Integrins

Integrins are a large family of multidomain α/β cell signaling receptors. Some integrins contain an additional inserted I domain, whose earliest expression appears to be with the chordates, since they are observed in the urochordates Ciona intestinalis (vase tunicate) and Halocynthia roretzi (sea pineapple), but not in integrins of earlier diverging species. The domain-s presence is viewed as a hallmark of integrins of higher metazoans, however in vertebrates, there are clearly three structurally-different classes: integrins without I domains, and two groups of integrins with I domains but separable by the presence or absence of an additional αC helix. For example, the αI domains in collagen-binding integrins from Osteichthyes (bony fish) and all higher vertebrates contain the specific αC helix, whereas the αI domains in non-collagen binding integrins from vertebrates and the αI domains from earlier diverging urochordate integrins, i.e. tunicates, do not. Unfortunately, within the early chordates, there is an evolutionary gap due to extinctions between the tunicates and cartilaginous fish. This, coupled with a knowledge gap due to the lack of complete genomic data from surviving species, means that the origin of collagen-binding αC-containing αI domains remains unknown. Here, we analyzed two available genomes from Callorhinchus milii (ghost shark/elephant shark; Chondrichthyes – cartilaginous fish) and Petromyzon marinus (sea lamprey; Agnathostomata), and several available Expression Sequence Tags from two Chondrichthyes species: Raja erinacea (little skate) and Squalus acanthias (dogfish shark); and Eptatretus burgeri (inshore hagfish; Agnathostomata), which evolutionary reside between the urochordates and osteichthyes. In P. marinus, we observed several fragments coding for the αC-containing αI domain, allowing us to shed more light on the evolution of the collagen-binding integrins.

Implementation of the SIP Express Router with Mediaproxy Method on VoIP

Voice Over IP (VoIP) is a technology that could pass the voice traffic and data packet form over an IP network. Network can be used for intranet or Internet. Phone calls using VoIP has advantages in terms of cheaper cost of PSTN phone to more than half, because the cost is calculated by the cost of the global nature of the Internet. Session Initiation Protocol (SIP) is a signaling protocol at the application layer which serves to establish, modify, and terminate a multimedia session involving one or more users. This SIP signaling has SIP message in text form that is used for session management by the SIP components, such as User Agent, Registrar, Redirect Server, and Proxy Server. To build a SIP communication is required SIP Express Router (SER) to be able to receive SIP messages, for handling the basic functions of SIP messages. Problems occur when the NAT through which affects the voice communication will be blocked starting from the sound that is not sent or one side of the sound are sent (half duplex). How that could be used to penetrate NAT is to use a given mediaproxy random RTP port to penetrate NAT.

Application of Simulation and Response Surface to Optimize Hospital Resources

This paper presents a case study that uses processoriented simulation to identify bottlenecks in the service delivery system in an emergency department of a hospital in the United Arab Emirates. Using results of the simulation, response surface models were developed to explain patient waiting time and the total time patients spend in the hospital system. Results of the study could be used as a service improvement tool to help hospital management in improving patient throughput and service quality in the hospital system.

Development of an Efficient CVT using Electromecanical System

Continuously variable transmission (CVT) is a type of automatic transmission that can change the gear ratio to any arbitrary setting within the limits. The most common type of CVT operates on a pulley system that allows an infinite variability between highest and lowest gears with no discrete steps. However, the current CVT system with hydraulic actuation method suffers from the power loss. It needs continuous force for the pulley to clamp the belt and hold the torque resulting in large amount of energy consumption. This study focused on the development of an electromechanical actuated control CVT to eliminate the problem that faced by the existing CVT. It is conducted with several steps; computing and selecting the appropriate sizing for stroke length, lead screw system and etc. From the visual observation it was found that the CVT system of this research is satisfactory.

Automation of Fishhooks Objective Measures

Fishing has always been an essential component of the Polynesians- life. Fishhooks, mostly in pearl shell, found during archaeological excavations are the artifacts related to this activity the most numerous. Thanks to them, we try to reconstruct the ancient techniques of resources exploitation, inside the lagoons and offshore. They can also be used as chronological and cultural indicators. The shapes and dimensions of these artifacts allow comparisons and classifications used in both functional approach and chrono-cultural perspective. Hence it is very important for the ethno-archaeologists to dispose of reliable methods and standardized measurement of these artifacts. Such a reliable objective and standardized method have been previously proposed. But this method cannot be envisaged manually because of the very important time required to measure each fishhook manually and the quantity of fishhooks to measure (many hundreds). We propose in this paper a detailed acquisition protocol of fishhooks and an automation of every step of this method. We also provide some experimental results obtained on the fishhooks coming from three archaeological excavations sites.

A Ring Segmented Bus Architecture for Globally Asynchronous Locally Synchronous System

Recently, most digital systems are designed as GALS (Globally Asynchronous Locally Synchronous) systems. Several architectures have been proposed as bus architectures for a GALS system : shared bus, segmented bus, ring bus, and so on. In this study, we propose a ring segmented bus architecture which is a combination of segmented bus and ring bus architecture with the aim of throughput enhancement. In a segmented bus architecture, segments are connected in series. By connecting the segments at the end of the bus and constructing the ring bus, it becomes possible to allocate a channel of the bus bidirectionally. The bus channel is allocated to the shortest path between segments. We consider a metastable operation caused by asynchronous communication between segments and a burst transfer between segments. According to the result of simulation, it is shown that the GALS system designed by the proposed method has the desired operations.

A Decision Support Tool for Evaluating Mobility Projects

Success is a European project that will implement several clean transport offers in three European cities and evaluate the environmental impacts. The goal of these measures is to improve urban mobility or the displacement of residents inside cities. For e.g. park and ride, electric vehicles, hybrid bus and bike sharing etc. A list of 28 criteria and 60 measures has been established for evaluation of these transport projects. The evaluation criteria can be grouped into: Transport, environment, social, economic and fuel consumption. This article proposes a decision support system based that encapsulates a hybrid approach based on fuzzy logic, multicriteria analysis and belief theory for the evaluation of impacts of urban mobility solutions. A web-based tool called DeSSIA (Decision Support System for Impacts Assessment) has been developed that treats complex data. The tool has several functionalities starting from data integration (import of data), evaluation of projects and finishes by graphical display of results. The tool development is based on the concept of MVC (Model, View, and Controller). The MVC is a conception model adapted to the creation of software's which impose separation between data, their treatment and presentation. Effort is laid on the ergonomic aspects of the application. It has codes compatible with the latest norms (XHTML, CSS) and has been validated by W3C (World Wide Web Consortium). The main ergonomic aspect focuses on the usability of the application, ease of learning and adoption. By the usage of technologies such as AJAX (XML and Java Script asynchrones), the application is more rapid and convivial. The positive points of our approach are that it treats heterogeneous data (qualitative, quantitative) from various information sources (human experts, survey, sensors, model etc.).

Application of Fuzzy Neural Network for Image Tumor Description

This paper used a fuzzy kohonen neural network for medical image segmentation. Image segmentation plays a important role in the many of medical imaging applications by automating or facilitating the diagnostic. The paper analyses the tumor by extraction of the features of (area, entropy, means and standard deviation).These measurements gives a description for a tumor.

Urban Land Cover Change of Olomouc City Using LANDSAT Images

This paper regards the phenomena of intensive suburbanization and urbanization in Olomouc city and in Olomouc region in general for the period of 1986–2009. A Remote Sensing approach that involves tracking of changes in Land Cover units is proposed to quantify the urbanization state and trends in temporal and spatial aspects. It actually consisted of two approaches, Experiment 1 and Experiment 2 which implied two different image classification solutions in order to provide Land Cover maps for each 1986–2009 time split available in the Landsat image set. Experiment 1 dealt with the unsupervised classification, while Experiment 2 involved semi- supervised classification, using a combination of object-based and pixel-based classifiers. The resulting Land Cover maps were subsequently quantified for the proportion of urban area unit and its trend through time, and also for the urban area unit stability, yielding the relation of spatial and temporal development of the urban area unit. Some outcomes seem promising but there is indisputably room for improvements of source data and also processing and filtering.

First Studies of the Influence of Single Gene Perturbations on the Inference of Genetic Networks

Inferring the network structure from time series data is a hard problem, especially if the time series is short and noisy. DNA microarray is a technology allowing to monitor the mRNA concentration of thousands of genes simultaneously that produces data of these characteristics. In this study we try to investigate the influence of the experimental design on the quality of the result. More precisely, we investigate the influence of two different types of random single gene perturbations on the inference of genetic networks from time series data. To obtain an objective quality measure for this influence we simulate gene expression values with a biologically plausible model of a known network structure. Within this framework we study the influence of single gene knock-outs in opposite to linearly controlled expression for single genes on the quality of the infered network structure.

Manufacturing Process of a Novel Biomass Composite Inspired from Cellular Structure of Wood

A novel biomass composite inspired from wood porous structure was manufactured by impregnating vinyl monomer into wood cellular structure under vacuum conditions, and initiating the monomer for in situ polymerization through a thermal treatment. The vacuum condition was studied, and the mechanical properties of the composite were also tested. SEM observation shows that polymer generated in the wood porous structure, and strongly interacted with wood matrix; and the polymer content increased with vacuum value increasing. FTIR indicates that polymer grafted onto wood matrix, resulting chemical complex between them. The rate of monomer loading increased with increasing vacuum value and time, accordance with rate of polymer loading. The compression strength and modulus of elasticity linearly increased with the increasing rate of polymer loading. Results indicate that the novel biomass composite possesses good mechanical properties capable of applying in the fields of construction, traffic and so forth.