In silico Simulations for DNA Shuffling Experiments

DNA shuffling is a powerful method used for in vitro evolute molecules with specific functions and has application in areas such as, for example, pharmaceutical, medical and agricultural research. The success of such experiments is dependent on a variety of parameters and conditions that, sometimes, can not be properly pre-established. Here, two computational models predicting DNA shuffling results is presented and their use and results are evaluated against an empirical experiment. The in silico and in vitro results show agreement indicating the importance of these two models and motivating the study and development of new models.

Energy Efficient Clustering and Data Aggregation in Wireless Sensor Networks

Wireless Sensor Networks (WSNs) are wireless networks consisting of number of tiny, low cost and low power sensor nodes to monitor various physical phenomena like temperature, pressure, vibration, landslide detection, presence of any object, etc. The major limitation in these networks is the use of nonrechargeable battery having limited power supply. The main cause of energy consumption WSN is communication subsystem. This paper presents an efficient grid formation/clustering strategy known as Grid based level Clustering and Aggregation of Data (GCAD). The proposed clustering strategy is simple and scalable that uses low duty cycle approach to keep non-CH nodes into sleep mode thus reducing energy consumption. Simulation results demonstrate that our proposed GCAD protocol performs better in various performance metrics.

Multiple Power Flow Solutions Using Particle Swarm Optimization with Embedded Local Search Technique

Particle Swarm Optimization (PSO) with elite PSO parameters has been developed for power flow analysis under practical constrained situations. Multiple solutions of the power flow problem are useful in voltage stability assessment of power system. A method of determination of multiple power flow solutions is presented using a hybrid of Particle Swarm Optimization (PSO) and local search technique. The unique and innovative learning factors of the PSO algorithm are formulated depending upon the node power mismatch values to be highly adaptive with the power flow problems. The local search is applied on the pbest solution obtained by the PSO algorithm in each iteration. The proposed algorithm performs reliably and provides multiple solutions when applied on standard and illconditioned systems. The test results show that the performances of the proposed algorithm under critical conditions are better than the conventional methods.

Design a Line Start synchronous Motor and Analysis Effect of the Rotor Structure on the Efficiency

The line start permanent magnet motor (LSPMM) combines a permanent magnet rotor for a better motor efficiency during synchronous running with an induction motor squirrel cage rotor to permit the motor starting by direct coupling to power source. In this paper effect of the rotor structure on a line start synchronous permanent magnet motor (LSPMM) is analyzed. LSPMM motor with three different structures for rotor is designed by using RMxprt software; efficiency and line current of LSPMM motor for different structures in full-load condition have been presented. The results indicate that with correct choosing of rotor structure, maximum efficiency can be found.

Generator Capability Curve Constraint for PSO Based Optimal Power Flow

An optimal power flow (OPF) based on particle swarm optimization (PSO) was developed with more realistic generator security constraint using the capability curve instead of only Pmin/Pmax and Qmin/Qmax. Neural network (NN) was used in designing digital capability curve and the security check algorithm. The algorithm is very simple and flexible especially for representing non linear generation operation limit near steady state stability limit and under excitation operation area. In effort to avoid local optimal power flow solution, the particle swarm optimization was implemented with enough widespread initial population. The objective function used in the optimization process is electric production cost which is dominated by fuel cost. The proposed method was implemented at Java Bali 500 kV power systems contain of 7 generators and 20 buses. The simulation result shows that the combination of generator power output resulted from the proposed method was more economic compared with the result using conventional constraint but operated at more marginal operating point.

Detection of Airborne Bacteria and Mildew in the Shanghai Metro System

This study aimed to detect and to identify the main strains of airborne microorganisms present in the Shanghai Metro system. Samples were collected using agar plates exposed to the air and microorganisms were identified using catalase, plasma coagulase and hymolytic analysis. The results show that the concentration of mildew present within a newly opened metro line was significantly higher than for other lines. Differences among underground and elevated stations can be attributed to differences in passenger flow and the environment surrounding the stations. Additionally, the investigation indicated that bacteria reached maximum levels at different times on weekdays and weekends. The bacteria in the Metro stations were identified as primarily Gram positive, consisting mainly of coagulase-negative staphylococcus strains (CNS).

Design and Economical Performance of Gray Water Treatment Plant in Rural Region

In India, the quarrel between the budding human populace and the planet-s unchanging supply of freshwater and falling water tables has strained attention the reuse of gray water as an alternative water resource in rural development. This paper present the finest design of laboratory scale gray water treatment plant, which is a combination of natural and physical operations such as primary settling with cascaded water flow, aeration, agitation and filtration, hence called as hybrid treatment process. The economical performance of the plant for treatment of bathrooms, basins and laundries gray water showed in terms of deduction competency of water pollutants such as COD (83%), TDS (70%), TSS (83%), total hardness (50%), oil and grease (97%), anions (46%) and cations (49%). Hence, this technology could be a good alternative to treat gray water in residential rural area.

Use of Bayesian Network in Information Extraction from Unstructured Data Sources

This paper applies Bayesian Networks to support information extraction from unstructured, ungrammatical, and incoherent data sources for semantic annotation. A tool has been developed that combines ontologies, machine learning, and information extraction and probabilistic reasoning techniques to support the extraction process. Data acquisition is performed with the aid of knowledge specified in the form of ontology. Due to the variable size of information available on different data sources, it is often the case that the extracted data contains missing values for certain variables of interest. It is desirable in such situations to predict the missing values. The methodology, presented in this paper, first learns a Bayesian network from the training data and then uses it to predict missing data and to resolve conflicts. Experiments have been conducted to analyze the performance of the presented methodology. The results look promising as the methodology achieves high degree of precision and recall for information extraction and reasonably good accuracy for predicting missing values.

Adaptive Impedance Control for Unknown Time-Varying Environment Position and Stiffness

This study is concerned with a new adaptive impedance control strategy to compensate for unknown time-varying environment stiffness and position. The uncertainties are expressed by Function Approximation Technique (FAT), which allows the update laws to be derived easily using Lyapunov stability theory. Computer simulation results are presented to validate the effectiveness of the proposed strategy.

Fuzzy Join Dependency in Fuzzy Relational Databases

The join dependency provides the basis for obtaining lossless join decomposition in a classical relational schema. The existence of Join dependency shows that that the tables always represent the correct data after being joined. Since the classical relational databases cannot handle imprecise data, they were extended to fuzzy relational databases so that uncertain, ambiguous, imprecise and partially known information can also be stored in databases in a formal way. However like classical databases, the fuzzy relational databases also undergoes decomposition during normalization, the issue of joining the decomposed fuzzy relations remains intact. Our effort in the present paper is to emphasize on this issue. In this paper we define fuzzy join dependency in the framework of type-1 fuzzy relational databases & type-2 fuzzy relational databases using the concept of fuzzy equality which is defined using fuzzy functions. We use the fuzzy equi-join operator for computing the fuzzy equality of two attribute values. We also discuss the dependency preservation property on execution of this fuzzy equi- join and derive the necessary condition for the fuzzy functional dependencies to be preserved on joining the decomposed fuzzy relations. We also derive the conditions for fuzzy join dependency to exist in context of both type-1 and type-2 fuzzy relational databases. We find that unlike the classical relational databases even the existence of a trivial join dependency does not ensure lossless join decomposition in type-2 fuzzy relational databases. Finally we derive the conditions for the fuzzy equality to be non zero and the qualification of an attribute for fuzzy key.

Artificial Accelerated Ageing Test of 22 kVXLPE Cable for Distribution System Applications in Thailand

This paper presents the experimental results on artificial ageing test of 22 kV XLPE cable for distribution system application in Thailand. XLPE insulating material of 22 kV cable was sliced to 60-70 μm in thick and was subjected to ac high voltage at 23 Ôùª C, 60 Ôùª C and 75 Ôùª C. Testing voltage was constantly applied to the specimen until breakdown. Breakdown voltage and time to breakdown were used to evaluate life time of insulating material. Furthermore, the physical model by J. P. Crine for predicts life time of XLPE insulating material was adopted as life time model and was calculated in order to compare the experimental results. Acceptable life time results were obtained from Crine-s model comparing with the experimental result. In addition, fourier transform infrared spectroscopy (FTIR) for chemical analysis and scanning electron microscope (SEM) for physical analysis were conducted on tested specimens.

Real-Time Detecting Concentration of Mycobacterium Tuberculosis by CNTFET Biosensor

Aptamers are useful tools in microorganism researches, diagnoses, and treatment. Aptamers are specific target molecules formed by oligonucleic acid molecules, and are not decomposed by alcohol. Aptamers used to detect Mycobacterium tuberculosis (MTB) have been proved to have specific affinity to the outer membrane proteins of MTB. This article presents a biosensor chip set with aptamers for early detection of MTB with high specificity and sensitivity, even in very low concentration. Meanwhile, we have already made a modified hydrophobic facial mask module with internal rendering hydrophobic for effectively collecting M. tuberculosis.

Modeling and Simulation of Robotic Arm Movement using Soft Computing

In this research paper we have presented control architecture for robotic arm movement and trajectory planning using Fuzzy Logic (FL) and Genetic Algorithms (GAs). This architecture is used to compensate the uncertainties like; movement, friction and settling time in robotic arm movement. The genetic algorithms and fuzzy logic is used to meet the objective of optimal control movement of robotic arm. This proposed technique represents a general model for redundant structures and may extend to other structures. Results show optimal angular movement of joints as result of evolutionary process. This technique has edge over the other techniques as minimum mathematics complexity used.

Supporting QoS-aware Multicasting in Differentiated Service Networks

A scalable QoS aware multicast deployment in DiffServ networks has become an important research dimension in recent years. Although multicasting and differentiated services are two complementary technologies, the integration of the two technologies is a non-trivial task due to architectural conflicts between them. A popular solution proposed is to extend the functionality of the DiffServ components to support multicasting. In this paper, we propose an algorithm to construct an efficient QoSdriven multicast tree, taking into account the available bandwidth per service class. We also present an efficient way to provision the limited available bandwidth for supporting heterogeneous users. The proposed mechanism is evaluated using simulated tests. The simulated result reveals that our algorithm can effectively minimize the bandwidth use and transmission cost

Robot Motion Planning in Dynamic Environments with Moving Obstacles and Target

This paper presents a new sensor-based online method for generating collision-free near-optimal paths for mobile robots pursuing a moving target amidst dynamic and static obstacles. At each iteration, first the set of all collision-free directions are calculated using velocity vectors of the robot relative to each obstacle and target, forming the Directive Circle (DC), which is a novel concept. Then, a direction close to the shortest path to the target is selected from feasible directions in DC. The DC prevents the robot from being trapped in deadlocks or local minima. It is assumed that the target's velocity is known, while the speeds of dynamic obstacles, as well as the locations of static obstacles, are to be calculated online. Extensive simulations and experimental results demonstrated the efficiency of the proposed method and its success in coping with complex environments and obstacles.

Physico-Mechanical Properties of Jute-Coir Fiber Reinforced Hybrid Polypropylene Composites

The term hybrid composite refers to the composite containing more than one type of fiber material as reinforcing fillers. It has become attractive structural material due to the ability of providing better combination of properties with respect to single fiber containing composite. The eco-friendly nature as well as processing advantage, light weight and low cost have enhanced the attraction and interest of natural fiber reinforced composite. The objective of present research is to study the mechanical properties of jute-coir fiber reinforced hybrid polypropylene (PP) composite according to filler loading variation. In the present work composites were manufactured by using hot press machine at four levels of fiber loading (5, 10, 15 and 20 wt %). Jute and coir fibers were utilized at a ratio of (1:1) during composite manufacturing. Tensile, flexural, impact and hardness tests were conducted for mechanical characterization. Tensile test of composite showed a decreasing trend of tensile strength and increasing trend of the Young-s modulus with increasing fiber content. During flexural, impact and hardness tests, the flexural strength, flexural modulus, impact strength and hardness were found to be increased with increasing fiber loading. Based on the fiber loading used in this study, 20% fiber reinforced composite resulted the best set of mechanical properties.

The Determination of Cellulose Spiral Angle by Small-Angle X-Ray Scattering from Structurally Characterized Acacia mangium Cell Wall

The spiral angle of the elementary cellulose fibril in the wood cell wall, often called microfibril angle, (MFA). Microfibril angle in hardwood is one of the key determinants of solid timber performance due to its strong influence on the stiffness, strength, shrinkage, swelling, thermal-dynamics mechanical properties and dimensional stability of wood. Variation of MFA (degree) in the S2 layer of the cell walls among Acacia mangium trees was determined using small-angle X-ray scattering (SAXS). The length and orientation of the microfibrils of the cell walls in the irradiated volume of the thin samples are measured using SAXS and optical microscope for 3D surface measurement. The undetermined parameters in the analysis are the MFA, (M) and the standard deviation (σФ) of the intensity distribution arising from the wandering of the fibril orientation about the mean value. Nine separate pairs of values are determined for nine different values of the angle of the incidence of the X-ray beam relative to the normal to the radial direction in the sample. The results show good agreement. The curve distribution of scattered intensity for the real cell wall structure is compared with that calculated with that assembly of rectangular cells with the same ratio of transverse to radial cell wall length. It is demonstrated that for β = 45°, the peaks in the curve intensity distribution for the real and the rectangular cells coincide. If this peak position is Ф45, then the MFA can be determined from the relation M = tan-1 (tan Ф45 / cos 45°), which is precise for rectangular cells. It was found that 92.93% of the variation of MFA can be attributed to the distance from pith to bark. Here we shall present our results of the MFA in the cell wall with respect to its shape, structure and the distance from pith to park as an important fast check and yet accurate towards the quality of wood, its uses and application.

Proposal of a Means for Reducing the Torque Variation on a Vertical-Axis Water Turbine by Increasing the Blade Number

This paper presents a means for reducing the torque variation during the revolution of a vertical-axis water turbine (VAWaterT) by increasing the blade number. For this purpose, twodimensional CFD analyses have been performed on a straight-bladed Darrieus-type rotor. After describing the computational model and the relative validation procedure, a complete campaign of simulations, based on full RANS unsteady calculations, is proposed for a three, four and five-bladed rotor architectures, characterized by a NACA 0025 airfoil. For each proposed rotor configuration, flow field characteristics are investigated at several values of tip speed ratio, allowing a quantification of the influence of blade number on flow geometric features and dynamic quantities, such as rotor torque and power. Finally, torque and power curves are compared for the three analyzed architectures, achieving a quantification of the effect of blade number on overall rotor performance.

Some Complexiton Type Solutions of the (3+1)-Dimensional Jimbo-Miwa Equation

By means of the extended homoclinic test approach (shortly EHTA) with the aid of a symbolic computation system such as Maple, some complexiton type solutions for the (3+1)-dimensional Jimbo-Miwa equation are presented.

The Intuitionistic Fuzzy Ordered Weighted Averaging-Weighted Average Operator and its Application in Financial Decision Making

We present a new intuitionistic fuzzy aggregation operator called the intuitionistic fuzzy ordered weighted averaging-weighted average (IFOWAWA) operator. The main advantage of the IFOWAWA operator is that it unifies the OWA operator with the WA in the same formulation considering the degree of importance that each concept has in the aggregation. Moreover, it is able to deal with an uncertain environment that can be assessed with intuitionistic fuzzy numbers. We study some of its main properties and we see that it has a lot of particular cases such as the intuitionistic fuzzy weighted average (IFWA) and the intuitionistic fuzzy OWA (IFOWA) operator. Finally, we study the applicability of the new approach on a financial decision making problem concerning the selection of financial strategies.