Abstract: This experimental study aims at studying the
conversion of macro-algae into bioethanol under several steps of
procedure: preparation, pre-treatment, fermentation, and distillation.
The main objective of this work was to investigate the role of buffer’s
type as a stabiliser of pH level and fermentation time on the yield of
ethanol. For this purpose, experiments were carried out on biomass
macro-algae to de-couple the pre-treatment and fermentation
processes from those associated with distillation process. β-
glucosidase was used as cellulose decomposer during hydrolysis step
and yeast was used during fermentation process. The species of
macro-algae utilised as energy feedstock was Ulva lactuca and it was
harvested from southern coast of Central of Java Island – Indonesia.
Experiments were conducted in a simple fermenter over a different
buffer: citrate buffer and acetic buffer, and over a range of
fermentation times between 5 to 20 days. The ethanol production was
found to be significantly affected by both variables. The optimum
time of fermentation was 10 days with citrate buffer; result in
0.88458% of ethanol, and the ethanol content after distillation
process was shown 0.985015%.
Abstract: The aim of present study was to monitor the presence
of Trichodina sp. in Rainbow trout, Oncorhynchus mykiss collected
from various fish farms in the western provinces of Iran during
January, 2013- January, 2014. Out of 675 sampled fish 335, (49.16%)
were infested with Trichodina. The highest prevalence was observed
in the spring and winter followed by autumn and summer. In general,
the intensity of infection was low except in cases where outbreaks of
Trichodiniasis endangered the survival of fish in some ponds. In light
infestation Trichodina is usually present on gills, fins and skin of
apparently healthy fish. Clinical signs of Trichodiniasis only appear
on fish with heavy infections and cases of moderate ones that are
usually exposed to one or more stress factors including, rough
handling during transportation from ponds, overcrowdness,
malnutrition, high of free ammonia and low of oxygen concentration.
Clinical signs of Trichodiniasis in sampled fish were sluggish
movement, loss of appetite, black coloration, necrosis and ulcer on
different parts of the body, detached scales and excessive
accumulation of mucous in gill pouches. The most obvious
histopathological changes in diseased fish were sloughing of the
epidermal layer, aggregation of leucocytes and melanine-carrying
cells (between the dermis and hypodermis) and proliferative changes
including hyperplasia and hypertrophy of the epithelial lining cells of
gill filaments which resulted in fusion of secondary lamellae. Control
of Trichodiniasis, has been achieved by formalin bath treatment at a
concentration of 250 ppm for one hour.
Abstract: This paper presents a real-time visualization technique
and filtering of classified LiDAR point clouds. The visualization is
capable of displaying filtered information organized in layers by the
classification attribute saved within LiDAR datasets. We explain the
used data structure and data management, which enables real-time
presentation of layered LiDAR data. Real-time visualization is
achieved with LOD optimization based on the distance from the
observer without loss of quality. The filtering process is done in two
steps and is entirely executed on the GPU and implemented using
programmable shaders.
Abstract: 3D part library is an ideal approach to reuse the
existing design and thus facilitates the modeling process, which will
enhance the efficiency. In this paper, we implemented the thought on
the SolidWorks platform. The system supports the functions of type
and parameter selection, 3D template driving and part assembly.
Finally, BOM is exported in Excel format. Experiment shows that our
method can satisfy the requirement of die and mold designers.
Abstract: Metallic foams have good potential for lightweight
structures for impact and blast mitigation. Therefore it is important to
find out the optimized foam structure (i.e. cell size, shape, relative
density, and distribution) to maximise energy absorption. In this
paper, quasistatic compression and microstructural characterization
of closed-cell aluminium foams of different pore size and cell
distributions have been carried out. We present results for two
different aluminium metal foams of density 0.49-0.51 g/cc and 0.31-
0.34 g/cc respectively that have been tested in quasi-static
compression. The influence of cell geometry and cell topology on
quasistatic compression behaviour has been investigated using optical
microscope and computed tomography (micro-CT) analysis. It is
shown that the deformation is not uniform in the structure and
collapse begins at the weakest point.
Abstract: Identification and selection of appropriate product and
manufacturing technologies are key factors for competitiveness and
market success of technology-based companies. Therefore, many
companies perform technology intelligence (TI) activities to ensure
the identification of evolving technologies at the right time.
Technology monitoring is one of the three base activities of TI,
besides scanning and scouting.
As the technological progress is accelerating, more and more
technologies are being developed. Against the background of limited
resources it is therefore necessary to focus TI activities. In this paper
we propose a concept for defining appropriate search fields for
technology monitoring. This limitation of search space leads to more
concentrated monitoring activities. The concept will be introduced
and demonstrated through an anonymized case study conducted
within an industry project at the Fraunhofer Institute for Production
Technology IPT.
The described concept provides a customized monitoring
approach, which is suitable for use in technology-oriented companies.
It is shown in this paper that the definition of search fields and search
tasks are suitable methods to define topics of interest and thus to
align monitoring activities. Current as well as planned product,
production and material technologies and existing skills, capabilities
and resources form the basis for derivation of relevant search areas.
To further improve the concept of technology monitoring the
proposed concept should be extended during future research e.g. by
the definition of relevant monitoring parameters.
Abstract: This paper presents a 4-DOF nonlinear model of a
cracked de Laval rotor-stator system derived based on Energy
Principles. The model has been used to simulate coupled torsionallateral
response of the faulty system with multiple parametric
excitations; rotor-stator-rub, a breathing transverse crack, eccentric
mass and an axial force. Nonlinearity of a “breathing” crack is
incorporated in the model using a simple hinge mechanism suitable
for a shallow crack. Response of the system while passing via its
critical speed with intermittent rotor-stator rub is analyzed. Effects of
eccentricity with phase and acceleration are investigated. Features of
crack, rub and eccentricity in vibration response are explored for
condition monitoring. The presence of a crack and rub are observable
in the power spectrum despite excitations by an axial force and rotor
unbalance. Obtained results are consistent with existing literature and
could be adopted into rotor condition monitoring strategies.
Abstract: The article discusses multimodal mobility in
contemporary societies as a main planning and organization issue in
the functioning of administrative bodies, a problem which really
exists in the space of contemporary cities in terms of shaping modern
transport systems. The article presents classification of available
resources and initiatives undertaken for developing multimodal
mobility. Solutions can be divided into three groups of measures –
physical measures in the form of changes of the transport network
infrastructure, organizational ones (including transport policy) and
information measures. The latter ones include in particular direct
support for people travelling in the transport network by providing
information about ways of using available means of transport. A
special measure contributing to this end is a trip planner. The article
compares several selected planners. It includes a short description of
the Green Travelling Project, which aims at developing a planner
supporting environmentally friendly solutions in terms of transport
network operation. The article summarizes preliminary findings of
the project.
Abstract: Immunomodulators are substances that alter immune
system via dynamic regulation of messenger molecules. It can be
divided into immunostimulant and immunosuppressant. It can help to
increase immunity of people with a low immune system, and also can
help to normalize an overactive immune system. Aim of this study is
to investigate the effects of in vitro exposure to low and high doses of
several immunomodulators which include caffeine, kaloba and
quercetin on antigen-stimulated whole blood culture cytokine
production. Whole blood samples were taken from 5 healthy males
(age: 32 ± 12 years; weight: 75.7 ± 6.1 kg; BMI: 24.3 ± 1.5 kg/m2)
following an overnight fast with no vigorous activity during the
preceding 24 h. The whole blood was then stimulated with 50 μl of
100 x diluted Pediacel vaccine and low or high dose of
immunomodulators in the culture plate. After 20 h incubation (5%
CO2, 37°C), it was analysed using the Evidence Investigator to
determine the production of cytokines including IL-2, IL-4, IL-10,
IFN-γ, and IL-1α. Caffeine and quercetin showed a tendency towards
decrease cytokine production as the doses were increased. On the
other hand, an upward trend was evident with kaloba, where a high
dose of kaloba seemed to increase the cytokine production. In
conclusion, we found that caffeine and quercetin have potential as
immunosuppressant and kaloba as immunostimulant.
Abstract: This research focuses on the optimization of glazed
surfaces and the assessment of possible solar gains in industrial
buildings. Existing window rating methods for single windows were
evaluated and a new method for a simple analysis of energy gains and
losses by single windows was introduced. Furthermore extensive
transient building simulations were carried out to appraise the
performance of low cost polycarbonate multi-cell sheets in
interaction with typical buildings for industrial applications. Mainly
energy saving potential was determined by optimizing the orientation
and area of such glazing systems in dependency on their thermal
qualities. Moreover the impact on critical aspects such as summer
overheating and daylight illumination was considered to ensure the
user comfort and avoid additional energy demand for lighting or
cooling. Hereby the simulated heating demand could be reduced by
up to 1/3 compared to traditional architecture of industrial halls using
mainly skylights.
Abstract: This paper presents a new method to design nonlinear
feedback linearization controller for PEMFCs (Polymer Electrolyte
Membrane Fuel Cells). A nonlinear controller is designed based on
nonlinear model to prolong the stack life of PEMFCs. Since it is
known that large deviations between hydrogen and oxygen partial
pressures can cause severe membrane damage in the fuel cell,
feedback linearization is applied to the PEMFC system so that the
deviation can be kept as small as possible during disturbances or load
variations. To obtain an accurate feedback linearization controller,
tuning the linear parameters are always important. So in proposed
study NSGA (Non-Dominated Sorting Genetic Algorithm)-II method
was used to tune the designed controller in aim to decrease the
controller tracking error. The simulation result showed that the
proposed method tuned the controller efficiently.
Abstract: Persea declinata (Bl.) Kosterm is a member of the
Lauraceae family, widely distributed in Southeast Asia. It is from the
same genus with avocado (Persea americana Mill), which is widely
consumed as food and for medicinal purposes. In the present study,
we examined the anticancer properties of Persea declinata (Bl.)
Kosterm bark methanolic crude extract (PDM). PDM exhibited a
potent antiproliferative effect in MCF-7 human breast cancer cells,
with an IC50 value of 16.68 .g/mL after 48h of treatment. We
observed that PDM caused cell cycle arrest and subsequent apoptosis
in MCF-7 cells, as exhibited by increased population at G0/G1 phase,
higher lactate dehydrogenase (LDH) release, and DNA
fragmentation. Mechanistic studies showed that PDM caused
significant elevation in ROS production, leading to perturbation of
mitochondrial membrane potential, cell permeability, and activation
of caspases-3/7. On the other hand, real-time PCR and Western blot
analysis showed that PDM treatment increased the expression of the
proapoptotic molecule, Bax, but decreased the expression of
prosurvival proteins, Bcl-2 and Bcl-xL, in a dose-dependent manner.
These findings imply that PDM could inhibit proliferation in MCF-7
cells via cell cycle arrest and apoptosis induction, indicating its
potential as a therapeutic agent worthy of further development.
Abstract: Calcium phosphate coating (CaP) has been employed
for protein delivery, but the typical direct protein adsorption on the
coating led to low incorporation content and fast release of the
protein from the coating. By using bovine serum albumin (BSA) as a
model protein, rapid biomimetic co-precipitation between calcium
phosphate and BSA was employed to control the distribution of BSA
within calcium phosphate coating during biomimetic formation on
titanium surface for only 6 h at 50oC in an accelerated calcium
phosphate solution. As a result, the amount of BSA incorporation and
release duration could be increased by using a rapid biomimetic coprecipitation
technique. Up to 43 fold increases in the BSA
incorporation content and the increase from 6 h to more than 360 h in
release duration compared to typical direct adsorption technique were
observed depending on the initial BSA concentration used during coprecipitation
(1, 10 and 100 μg.ml-1). From x-ray diffraction and
Fourier transform infrared spectroscopy studies, the coating
composition was not altered with the incorporation of BSA by this
rapid biomimetic co-precipitation and mainly comprised octacalcium
phosphate and hydroxyapatite. However, the microstructure of
calcium phosphate crystals changed from straight, plate-like units to
curved, plate-like units with increasing BSA content.
Abstract: To mimic the natural circumstances of cell growth in an organism, we present three-dimensional (3D) scaffolds fabricated by microfluidics for cultivation. This work investigates the cellular behaviors of rat cardiomyocytes in gelatin 3D scaffolds compared to those on 2D control, such as proliferation, viability and morphology. We found that the scaffolds may induce skeletal differentiation of H9c2 cells.
Abstract: Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.
Abstract: This study focuses on the cooling of a photovoltaic
panel (PV). Indeed, the cooling improves the conversion capacity of
this one and maintains, under extreme conditions of air temperature,
the panel temperature at an appreciable level which avoids the
altering. To do this, a fan provides forced circulation of air. Because
the fan is supplied by the panel, it is necessary to determine the
optimum operating point that unites efficiency of the PV with the
consumption of the fan. For this matter, numerical simulations are
performed at varying mass flow rates of air, under two extreme air
temperatures (50°C, 25°C) and a fixed solar radiation (1000W.m2) in
a case of no wind.
Abstract: Malathion (ML) is a well known pesticide commonly
used in many agricultural and non-agricultural processes. Its toxicity
has been attributed primarily to the accumulation of acetylcholine
(Ach) at nerve junctions, due to the inhibition of acetylcholinesterase
(AChE). The aim of the current research was to study the protective
effect of the melissa plant extract against reproductive impairment
induced by malathion in 32 male albino rats, and the biological
experiment was divided into four groups (8 in each) that given
malathion (27 mg/kg; 1/50 of the LD50 for an oral dose) and/or
Melissa officinalis (MO) extract (200mg/kg/day) by gavages
technique. The sperm counts, sperm motility, sperm morphology,
FSH, LH, and testosterone levels had been determined in testes
homogenate at the end of the experiment. It is worthy to report that,
rats treated with melissa extract did not show a significant difference
when compared with the control group, while rats given malathion
alone had significantly lower sperm count, sperm motility, and
significantly higher abnormal sperm numbers, than the untreated
control rats as well as having significantly lower serum FSH, LH, and
testosterone levels compared with the control group. Administrations
of melissa extract restore all mentioned histological parameters
towards the control group and the melissa extract had a strong
positive protective effect against malathion toxicity. Results the of
biological parameters were confirmed by the histological
examination of rat testes and indicated that, both control and melissa
groups showing normal seminiferous tubules, while malathion group
testicular tissues had necrosis, edema in the seminiferous tubules and
degeneration of spermatogonial cells lining the seminiferous tubules
with incomplete spermatogenesis. The use of melissa against
malathion improved the histological picture and showing normal
seminiferous tubules with complete spermatogenesis and almost there
was no histopathological changes could be noted.
Abstract: Currently, biological control programs in greenhouse
crops involve the use, at the same time, several natural enemies
during the crop cycle. Also, large number of plant species grown in
greenhouses, among them, the used cultivars are also wide. However,
the cultivar effects on entomophagous species efficacy (predators and
parasitoids) have been scarcely studied. A new method had been
developed, using the factitious prey or host Ephestia kuehniella. It
allow us to evaluate, under greenhouse or controlled conditions
(semi-field), the cultivar effects on the entomophagous species
effectiveness. The work was carried out in greenhouse tomato crop. It
has been found the biological and ecological activities of predatory
species (Nesidiocoris tenuis) and egg-parasitoid (Trichogramma
achaeae) can be well represented with the use of the factitious prey
or host; being better in the former than the latter. The data found in
the trial are shown and discussed. The developed method could be
applied to evaluate new plant materials before making available to
farmers as commercial varieties, at low costs and easy use.
Abstract: Two types of commercial cylindrical lithium ion
batteries (Panasonic 3.4 Ah NCR-18650B and Samsung 2.9 Ah
INR-18650), were investigated experimentally. The capacities of these
samples were individually measured using constant current-constant
voltage (CC-CV) method at different ambient temperatures (-10°C,
0°C, 25°C). Their internal resistance was determined by
electrochemical impedance spectroscopy (EIS) and pulse discharge
methods. The cells with different configurations of parallel connection
NCR-NCR, INR-INR and NCR-INR were charged/discharged at the
aforementioned ambient temperatures. The results showed that the
difference of internal resistance between cells much more evident at
low temperatures. Furthermore, the parallel connection of NCR-NCR
exhibits the most uniform temperature distribution in cells at -10°C,
this feature is quite favorable for the safety of the battery pack.
Abstract: Recent perceived climate variability raises concerns
with unprecedented hydrological phenomena and extremes.
Distribution and circulation of the waters of the Earth become
increasingly difficult to determine because of additional uncertainty
related to anthropogenic emissions. The world wide observed
changes in the large-scale hydrological cycle have been related to an
increase in the observed temperature over several decades. Although
the effect of change in climate on hydrology provides a general
picture of possible hydrological global change, new tools and
frameworks for modelling hydrological series with nonstationary
characteristics at finer scales, are required for assessing climate
change impacts. Of the downscaling techniques, dynamic
downscaling is usually based on the use of Regional Climate Models
(RCMs), which generate finer resolution output based on atmospheric
physics over a region using General Circulation Model (GCM) fields
as boundary conditions. However, RCMs are not expected to capture
the observed spatial precipitation extremes at a fine cell scale or at a
basin scale. Statistical downscaling derives a statistical or empirical
relationship between the variables simulated by the GCMs, called
predictors, and station-scale hydrologic variables, called predictands.
The main focus of the paper is on the need for using statistical
downscaling techniques for projection of local hydrometeorological
variables under climate change scenarios. The projections can be then
served as a means of input source to various hydrologic models to
obtain streamflow, evapotranspiration, soil moisture and other
hydrological variables of interest.