Abstract: Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.
Abstract: 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate using NADPH and the enzyme is involved in rate-controlling step of mevalonate. Inhibition of HMGR is considered as effective way to lower cholesterol levels so it is drug target to treat hypercholesterolemia, major risk factor of cardiovascular disease. To discover novel HMGR inhibitor, we performed structure-based pharmacophore modeling combined with molecular dynamics (MD) simulation. Four HMGR inhibitors were used for MD simulation and representative structure of each simulation were selected by clustering analysis. Four structure-based pharmacophore models were generated using the representative structure. The generated models were validated used in virtual screening to find novel scaffolds for inhibiting HMGR. The screened compounds were filtered by applying drug-like properties and used in molecular docking. Finally, four hit compounds were obtained and these complexes were refined using energy minimization. These compounds might be potential leads to design novel HMGR inhibitor.
Abstract: In single trial analysis, when using Principal
Component Analysis (PCA) to extract Visual Evoked Potential
(VEP) signals, the selection of principal components (PCs) is an
important issue. We propose a new method here that selects only
the appropriate PCs. We denote the method as selective eigen-rate
(SER). In the method, the VEP is reconstructed based on the rate
of the eigen-values of the PCs. When this technique is applied on
emulated VEP signals added with background
electroencephalogram (EEG), with a focus on extracting the
evoked P3 parameter, it is found to be feasible. The improvement
in signal to noise ratio (SNR) is superior to two other existing
methods of PC selection: Kaiser (KSR) and Residual Power (RP).
Though another PC selection method, Spectral Power Ratio (SPR)
gives a comparable SNR with high noise factors (i.e. EEGs), SER
give more impressive results in such cases. Next, we applied SER
method to real VEP signals to analyse the P3 responses for
matched and non-matched stimuli. The P3 parameters extracted
through our proposed SER method showed higher P3 response for
matched stimulus, which confirms to the existing neuroscience
knowledge. Single trial PCA using KSR and RP methods failed to
indicate any difference for the stimuli.
Abstract: Grid composite structures have many applications in aerospace industry in which deal with transverse loadings abundantly. In present paper a stiffened composite cylindrical shell with clamped-free boundary condition under transverse end load experimentally and numerically was studied. Some electrical strain gauges were employed to measure the strains. Also a finite element analysis was done for validation of experimental result. The FEM software used was ANSYS11. In addition, the results between stiffened composite shell and unstiffened composite shell were compared. It was observed that intersection of two stiffeners has an important effect in decrease of stress in the shell. Fairly good agreements were observed between the numerical and the measured results. According to recent studies about grid composite structures, it should be noted that any investigation like this research has not been reported.
Abstract: There are three distinct stages in the evolution of
economic thought, namely:
1. in the first stage, the major concern was to accelerate
economic growth with increased availability of material
goods, especially in developing economies with very low
living standards, because poverty eradication meant faster
economic growth.
2. in the second stage, economists made distinction between
growth and development. Development was seen as going
beyond economic growth, and bringing certain changes in
the structure of the economy with more equitable
distribution of the benefits of growth, with the growth
coming automatic and sustained.
3. the third stage is now reached. Our concern is now with
“sustainable development", that is, development not only
for the present but also of the future.
Thus the focus changed from “sustained growth" to “sustained
development". Sustained development brings to the fore the long
term relationship between the ecology and economic development.
Since the creation of UNEP in 1972 it has worked for
development without destruction for environmentally sound and
sustained development. It was realised that the environment cannot
be viewed in a vaccum, it is not separate from development, nor is it
competing. It suggested for the integration of the environment with
development whereby ecological factors enter development planning,
socio-economic policies, cost-benefit analysis, trade, technology
transfer, waste management, educational and other specific areas.
Industrialisation has contributed to the growth of economy of
several countries. It has improved the standards of living of its people
and provided benefits to the society. It has also created in the process
great environmental problems like climate change, forest destruction
and denudation, soil erosion and desertification etc.
On the other hand, industry has provided jobs and improved the
prospects of wealth for the industrialists. The working class
communities had to simply put up with the high levels of pollution in
order to keep up their jobs and also to save their income.
There are many roots of the environmental problem. They may be
political, economic, cultural and technological conditions of the
modern society. The experts concede that industrial growth lies
somewhere close to the heart of the matter. Therefore, the objective
of this paper is not to document all roots of an environmental crisis
but rather to discuss the effects of industrial growth and
development.
We have come to the conclusion that although public intervention
is often unnecessary to ensure that perfectly competitive markets will
function in society-s best interests, such intervention is necessary
when firms or consumers pollute.
Abstract: In this paper we propose a computational model for the representation and processing of morpho-phonological phenomena in a natural language, like Modern Greek. We aim at a unified treatment of inflection, compounding, and word-internal phonological changes, in a model that is used for both analysis and generation. After discussing certain difficulties cuase by well-known finitestate approaches, such as Koskenniemi-s two-level model [7] when applied to a computational treatment of compounding, we argue that a morphology-based model provides a more adequate account of word-internal phenomena. Contrary to the finite state approaches that cannot handle hierarchical word constituency in a satisfactory way, we propose a unification-based word grammar, as the nucleus of our strategy, which takes into consideration word representations that are based on affixation and [stem stem] or [stem word] compounds. In our formalism, feature-passing operations are formulated with the use of the unification device, and phonological rules modeling the correspondence between lexical and surface forms apply at morpheme boundaries. In the paper, examples from Modern Greek illustrate our approach. Morpheme structures, stress, and morphologically conditioned phoneme changes are analyzed and generated in a principled way.
Abstract: Identifying the nature of protein-nanoparticle
interactions and favored binding sites is an important issue in
functional characterization of biomolecules and their physiological
responses. Herein, interaction of silver nanoparticles with lysozyme
as a model protein has been monitored via fluorescence spectroscopy.
Formation of complex between the biomolecule and silver
nanoparticles (AgNPs) induced a steady state reduction in the
fluorescence intensity of protein at different concentrations of
nanoparticles. Tryptophan fluorescence quenching spectra suggested
that silver nanoparticles act as a foreign quencher, approaching the
protein via this residue. Analysis of the Stern-Volmer plot showed
quenching constant of 3.73 μM−1. Moreover, a single binding site in
lysozyme is suggested to play role during interaction with AgNPs,
having low affinity of binding compared to gold nanoparticles.
Unfolding studies of lysozyme showed that complex of lysozyme-
AgNPs has not undergone structural perturbations compared to the
bare protein. Results of this effort will pave the way for utilization of
sensitive spectroscopic techniques for rational design of
nanobiomaterials in biomedical applications.
Abstract: Using vision based solution in intelligent vehicle application often needs large memory to handle video stream and image process which increase complexity of hardware and software. In this paper, we present a FPGA implement of a vision based lane departure warning system. By taking frame of videos, the line gradient of line is estimated and the lane marks are found. By analysis the position of lane mark, departure of vehicle will be detected in time. This idea has been implemented in Xilinx Spartan6 FPGA. The lane departure warning system used 39% logic resources and no memory of the device. The average availability is 92.5%. The frame rate is more than 30 frames per second (fps).
Abstract: To investigate the correspondence of theory and
practice, a successfully implemented Knowledge Management
System (KMS) is explored through the lens of Alavi and Leidner-s
proposed KMS framework for the analysis of an information system
in knowledge management (Framework-AISKM). The applied KMS
system was designed to manage curricular knowledge in a distributed
university environment. The motivation for the KMS is discussed
along with the types of knowledge necessary in an academic setting.
Elements of the KMS involved in all phases of capturing and
disseminating knowledge are described. As the KMS matures the
resulting data stores form the precursor to and the potential for
knowledge mining. The findings from this exploratory study indicate
substantial correspondence between the successful KMS and the
theory-based framework providing provisional confirmation for the
framework while suggesting factors that contributed to the system-s
success. Avenues for future work are described.
Abstract: Reservoirs with high pressures and temperatures
(HPHT) that were considered to be atypical in the past are now
frequent targets for exploration. For downhole oilfield drilling tools
and components, the temperature and pressure affect the mechanical
strength. To address this issue, a finite element analysis (FEA) for
206.84 MPa (30 ksi) pressure and 165°C has been performed on the
pressure housing of the measurement-while-drilling/logging-whiledrilling
(MWD/LWD) density tool.
The density tool is a MWD/LWD sensor that measures the density
of the formation. One of the components of the density tool is the
pressure housing that is positioned in the tool. The FEA results are
compared with the experimental test performed on the pressure
housing of the density tool. Past results show a close match between
the numerical results and the experimental test. This FEA model can
be used for extreme HPHT and ultra HPHT analyses, and/or optimal
design changes.
Abstract: Mobile IP has been developed to provide the
continuous information network access to mobile users. In IP-based
mobile networks, location management is an important component of
mobility management. This management enables the system to track
the location of mobile node between consecutive communications. It
includes two important tasks- location update and call delivery.
Location update is associated with signaling load. Frequent updates
lead to degradation in the overall performance of the network and the
underutilization of the resources. It is, therefore, required to devise
the mechanism to minimize the update rate. Mobile IPv6 (MIPv6)
and Hierarchical MIPv6 (HMIPv6) have been the potential
candidates for deployments in mobile IP networks for mobility
management. HMIPv6 through studies has been shown with better
performance as compared to MIPv6. It reduces the signaling
overhead traffic by making registration process local. In this paper,
we present performance analysis of MIPv6 and HMIPv6 using an
analytical model. Location update cost function is formulated based
on fluid flow mobility model. The impact of cell residence time, cell
residence probability and user-s mobility is investigated. Numerical
results are obtained and presented in graphical form. It is shown that
HMIPv6 outperforms MIPv6 for high mobility users only and for low
mobility users; performance of both the schemes is almost equivalent
to each other.
Abstract: In this paper, we present a new algorithm for clustering data in large datasets using image processing approaches. First the dataset is mapped into a binary image plane. The synthesized image is then processed utilizing efficient image processing techniques to cluster the data in the dataset. Henceforth, the algorithm avoids exhaustive search to identify clusters. The algorithm considers only a small set of the data that contains critical boundary information sufficient to identify contained clusters. Compared to available data clustering techniques, the proposed algorithm produces similar quality results and outperforms them in execution time and storage requirements.
Abstract: Recently, X. Ge and J. Qian investigated some relations between higher mathematics scores and calculus scores (resp. linear algebra scores, probability statistics scores) for Chinese university students. Based on rough-set theory, they established an information system S = (U,CuD,V, f). In this information system, higher mathematics score was taken as a decision attribute and calculus score, linear algebra score, probability statistics score were taken as condition attributes. They investigated importance of each condition attribute with respective to decision attribute and strength of each condition attribute supporting decision attribute. In this paper, we give further investigations for this issue. Based on the above information system S = (U, CU D, V, f), we analyze the decision rules between condition and decision granules. For each x E U, we obtain support (resp. strength, certainty factor, coverage factor) of the decision rule C —>x D, where C —>x D is the decision rule induced by x in S = (U, CU D, V, f). Results of this paper gives new analysis of on higher mathematics scores for Chinese university students, which can further lead Chinese university students to raise higher mathematics scores in Chinese graduate student entrance examination.
Abstract: Due to today-s fierce competition, companies have to
be proactive creators of the future by effectively developing
innovations. Especially radical innovations allow high profit margins
– but they also entail high risks. One possibility to realize radical
innovations and reduce the risk of failure is cross-industry innovation
(CII). CII brings together problems and solution ideas from different
industries. However, there is a lack of systematic ways towards CII.
Bridging this gap, the present paper provides a systematic approach
towards planned CII. Starting with the analysis of potentials, the
definition of promising search strategies is crucial. Subsequently,
identified solution ideas need to be assessed. For the most promising
ones, the adaption process has to be systematically planned –
regarding the risk affinity of a company. The introduced method is
explained on a project from the furniture industry.
Abstract: Small cracks or chips of a product appear very
frequently in the course of continuous production of an automatic
press process system. These phenomena become the cause of not only
defective product but also damage of a press mold. In order to solve
this problem AE system was introduced. AE system was expected to
be very effective to real time detection of the defective product and to
prevention of the damage of the press molds.
In this study, for pick and analysis of AE signals generated from the
press process, AE sensors/pre-amplifier/analysis and processing board
were used as frequently found in the other similar cases. For analysis
and processing the AE signals picked in real time from the good or bad
products, specialized software called cdm8 was used. As a result of
this work it was conformed that intensity and shape of the various AE
signals differ depending on the weight and thickness of metal sheet
and process type.
Abstract: Bovine viral diarrhea virus (BVDV) can cause lifelong
persistent infection. One reason for the phenomena is attributed to
BVDV infection to placenta tissue. However the mechanisms that
BVDV invades into placenta tissue remain unclear. To clarify the
molecular mechanisms, we investigated the possible means that
BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid
system was used to identify proteins extracted from TPC, which
interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast
expression vector and TPC cDNA library were constructed. Through
two rounds of screening, three positive clones were identified.
Sequencing analysis indicated that all the three positive clones
encoded the same protein clathrin. Physical interaction between
clathrin and BVDV E2 protein was further confirmed by
coimmunoprecipitation experiments. This result suggested that the
clathrin might play a critical role in the process of BVDV entry into
placenta tissue and might be a novel antiviral target for preventing
BVDV infection.
Abstract: Long term rainfall analysis and prediction is a
challenging task especially in the modern world where the impact of
global warming is creating complications in environmental issues.
These factors which are data intensive require high performance
computational modeling for accurate prediction. This research paper
describes a prototype which is designed and developed on grid
environment using a number of coupled software infrastructural
building blocks. This grid enabled system provides the demanding
computational power, efficiency, resources, user-friendly interface,
secured job submission and high throughput. The results obtained
using sequential execution and grid enabled execution shows that
computational performance has enhanced among 36% to 75%, for
decade of climate parameters. Large variation in performance can be
attributed to varying degree of computational resources available for
job execution.
Grid Computing enables the dynamic runtime selection, sharing
and aggregation of distributed and autonomous resources which plays
an important role not only in business, but also in scientific
implications and social surroundings. This research paper attempts to
explore the grid enabled computing capabilities on weather indices
from HOAPS data for climate impact modeling and change
detection.
Abstract: The Mobile Ad-hoc Network (MANET) is a collection of self-configuring and rapidly deployed mobile nodes (routers) without any central infrastructure. Routing is one of the potential issues. Many routing protocols are reported but it is difficult to decide which one is best in all scenarios. In this paper on demand routing protocols DSR and DYMO based on IEEE 802.11 DCF MAC protocol are examined and characteristic summary of these routing protocols is presented. Their performance is analyzed and compared on performance measuring metrics throughput, dropped packets due to non availability of routes, duplicate RREQ generated for route discovery and normalized routing load by varying CBR data traffic load using QualNet 5.0.2 network simulator.
Abstract: This paper studies the mean square exponential synchronization problem of a class of stochastic neutral type chaotic neural networks with mixed delay. On the Basis of Lyapunov stability theory, some sufficient conditions ensuring the mean square exponential synchronization of two identical chaotic neural networks are obtained by using stochastic analysis and inequality technique. These conditions are expressed in the form of linear matrix inequalities (LMIs), whose feasibility can be easily checked by using Matlab LMI Toolbox. The feedback controller used in this paper is more general than those used in previous literatures. One simulation example is presented to demonstrate the effectiveness of the derived results.
Abstract: Data envelopment analysis (DEA) has gained great popularity in environmental performance measurement because it can provide a synthetic standardized environmental performance index when pollutants are suitably incorporated into the traditional DEA framework. Since some of the environmental performance indicators cannot be controlled by companies managers, it is necessary to develop the model in a way that it could be applied when discretionary and/or non-discretionary factors were involved. In this paper, we present a semi-radial DEA approach to measuring environmental performance, which consists of non-discretionary factors. The model, then, has been applied on a real case.