Abstract: Sediment and mangrove root samples from Iko River
Estuary, Nigeria were analyzed for microbial and polycyclic
aromatic hydrocarbon (PAH) content. The total heterotrophic
bacterial (THB) count ranged from 1.1x107 to 5.1 x107 cfu/g, total
fungal (TF) count ranged from 1.0x106 to 2.7x106 cfu/g, total
coliform (TC) count ranged from 2.0x104 to 8.0x104cfu/g while
hydrocarbon utilizing bacterial (HUB) count ranged from 1.0x 105 to
5.0 x 105cfu/g. There was a range of positive correlation (r = 0.72 to
0.93) between THB count and total HUB count, respectively. The
organisms were Staphylococcus aureus, Bacillus cereus,
Flavobacterium breve, Pseudomonas aeruginosa, Erwinia
amylovora, Escherichia coli, Enterobacter sp, Desulfovibrio sp,
Acinetobacter iwoffii, Chromobacterium violaceum, Micrococcus
sedentarius, Corynebacterium sp, and Pseudomonas putrefaciens.
The PAH were Naphthalene, 2-Methylnaphthalene, Acenapthylene,
Acenaphthene, Fluorene, Phenanthene, Anthracene, Fluoranthene,
Pyrene, Benzo(a)anthracene, Chrysene, Benzo(b)fluoranthene,
Benzo(k)fluoranthene, Benzo(a)pyrene, Dibenzo(a,h)anthracene,
Benzo(g,h,l)perylene ,Indeno(1,2,3-d)pyrene with individual PAH
concentrations that ranged from 0.20mg/kg to 1.02mg/kg, 0.20mg/kg
to 1.07mg/kg and 0.2mg/kg to 4.43mg/kg in the benthic sediment,
epipellic sediment and mangrove roots, respectively. Total PAH
ranged from 6.30 to 9.93mg/kg, 6.30 to 9.13mg/kg and 9.66 to
16.68mg/kg in the benthic sediment, epipellic sediment and
mangrove roots, respectively. The high concentrations in the
mangrove roots are indicative of bioaccumulation of the pollutant in
the plant tissue. The microorganisms are of ecological significance
and the detectable quantities of polycyclic aromatic hydrocarbon
could be partitioned and accumulated in tissues of infaunal and
epifaunal organisms in the study area.
Abstract: The purpose of this study was to elucidate the factors affecting antimicrobial effectiveness of essential oils against food spoilage and pathogenic bacteria. The minimum inhibition concentrations (MIC) of the essential oils, were determined by turbidimetric technique using Biocreen C, analyzer. The effects of pH ranging from 7.3 to 5.5 in absence and presence of essential oils and/or NaCl on the lag time and mean generation time of the bacteria at 370C, were carried out and results were determined showed that, combination of low pH and essential oil at 370C had additive effects against the test micro-organisms. The combination of 1.2 % (w/v) of NaCl and clove essential oil at 0.0325% (v/v) was effective against E. coli. The use of concentrations less than MIC in combination with low pH and or NaCl has the potential of being used as an alternative to “traditional food preservatives".
Abstract: This study applied the Gaussian trajectory
transfer-coefficient model (GTx) to simulate the particulate matter
concentrations and the source apportionments at Nanzih Air Quality
Monitoring Station in southern Taiwan from November 2007 to
February 2008. The correlation coefficient between the observed and
the calculated daily PM10 concentrations is 0.5 and the absolute bias of
the PM10 concentrations is 24%. The simulated PM10 concentrations
matched well with the observed data. Although the emission rate of
PM10 was dominated by area sources (58%), the results of source
apportionments indicated that the primary sources for PM10 at Nanzih
Station were point sources (42%), area sources (20%) and then upwind
boundary concentration (14%). The obvious difference of PM10 source
apportionment between episode and non-episode days was upwind
boundary concentrations which contributed to 20% and 11% PM10
sources, respectively. The gas-particle conversion of secondary
aerosol and long range transport played crucial roles on the PM10
contribution to a receptor.
Abstract: A comparative evaluation of acute toxicity of
synthesized nano silvers using two different procedures (biological
and chemical reduction methods) and silver ions on bacteria
Vibrio fischeri was investigated. The bacterial light inhibition test as
a toxicological endpoint was used by applying of a homemade
luminometer. To compare the toxicity effects as a quantitative
parameter, a nominal effective concentrations (EC) of chemicals and
a susceptibility constant (Z-value) of bacteria, after 5 min and 30 min
exposure times, were calculated. After 5 and 30 min contact times,
the EC50 values of two silver nanoparticles and the EC20 values were
about similar. It demonstrates that toxicity of silvers was independent
of their procedure. The EC values of nanoparticles were larger than
those of the silver ions. The susceptibilities(Z- Values) of V.fischeri
(L/mg) to the silver ions were greater than those of the nano silvers.
According to the EC and Z values, the toxicity of silvers decreased in
the following order: Silver ions >> silver nanoparticles from
chemical reduction method ~ silver nanoparticles from biological
method.
Abstract: The evolution of silica optical fiber strength aged in cetyltrimethylammonium chloride solution (CTAC) has been investigated. If the solution containing surfactants presents appreciable changes in physical and chemical properties at the critical micelle concentration (CMC), a non negligible mechanical behavior fiber change is observed for silica fiber aged in cationic surfactants as CTAC which can lead to optical fiber reliability questioning. The purpose of this work is to study the mechanical behavior of silica coated and naked optical fibers in contact with CTAC solution at different concentrations. Result analysis proves that the immersion in CTAC drastically decreases the fiber strength and specially near the CMC point. Beyond CMC point, a small increase of fiber strength is analyzed and commented.
Abstract: The aim of the present study was to evaluate the
mucoadhesion and the release of nicotinamide gel formulations using
in vitro methods. An agar plate technique was used to investigate the
adhesiveness of the gels whereas a diffusion apparatus was employed
to determine the release of nicotinamide from the gels. In this
respect, 10% w/w nicotinamide gels containing bioadhesive
polymers: Carbopol 934P (0.5-2% w/w), hydroxypropylmethyl
cellulose (HPMC) (4-10% w/w), sodium carboxymethyl cellulose
(SCMC) (4-6% w/w) and methylcellulose 4000 (MC) (3-5% w/w)
were prepared. The gel formulations had pH values in the range of
7.14 - 8.17, which were considered appropriate to oral mucosa
application. In general, the rank order of pH values appeared to be
SCMC > MC4000 > HPMC > Carbopol 934P. Types and
concentrations of polymers used somewhat affected the
adhesiveness. It was found that anionic polymers (Carbopol 934 and
SCMC) adhered more firmly to the agar plate than the neutral
polymers (HPMC and MC 4000). The formulation containing 0.5%
Carbopol 934P (F1) showed the highest release rate. With the
exception of the formulation F1, the neutral polymers tended to give
higher relate rates than the anionic polymers. For oral tissue
treatment, the optimum has to be balanced between the residence
time (adhesiveness) of the formulations and the release rate of the
drug. The formulations containing the anionic polymers: Carbopol
934P or SCMC possessed suitable physical properties (appearance,
pH and viscosity). In addition, for anionic polymer formulations,
justifiable mucoadhesive properties and reasonable release rates of
nicotinamide were achieved. Accordingly, these gel formulations
may be applied for the treatment of oral mucosal lesions.
Abstract: Surface sediment samples were collected from the
Canon River mouth, Taiwan and analyzed for polycyclic aromatic
hydrocarbons (PAHs). Total PAHs concentrations varied from 337 to
1,252 ng/g dry weight, with a mean concentration of 827 ng/g dry
weight. The spatial distribution of PAHs reveals that the PAHs
concentration is relatively high in the river mouth region, and
gradually diminishes toward the harbor region. Diagnostic ratios
showed that the possible source of PAHs in the Canon River mouth
could be petroleum combustion. The toxic equivalent concentrations
(TEQcarc) of PAHs varied from 47 to 112 ng TEQ/g dry weight. Higher
total TEQcarc values were found in the river mouth region. As
compared with the US Sediment Quality Guidelines (SQGs), the
observed levels of PAHs at Canon River mouth were lower than the
effects range low (ERL), and would probably not exert adverse
biological effects.
Abstract: The acid rain causes change in pH level of soil it is
directly influence on root and leaf growth. Yield of the crop was
reduced if acidity of soil is more. Acid rain seeps into the earth and
poisons plants and trees by dissolving toxic substances in the soil,
such as aluminum, which get absorbed by the roots. In present
investigation, effect of acid rain on crop Vigna radiata was studied.
The effect of acid rain on change in soil fertility was detected in
which pH of control sample was 6.5 and pH of 1% H2SO4 and 1%
HNO3 were 3.5. Nitrogen nitrate in soil was high in 1% HNO3 treated
soil & Control sample. Ammonium nitrogen in soil was low in 1%
HNO3 & H2SO4 treated soil. Ammonium nitrogen was medium in
control and other samples. The effect of acid rain on seed
germination on 3rd day of germination control sample growth was
6.1cm with plumule 0.001% HNO3 & 0.001% H2SO4 was 5.5cm
with plumule and 8cm with plumule. On 10th day fungal growth was
observed in 1% and 0.1% H2SO4 concentrations when all plants were
dead. The effect of acid rain on crop productivity was investigated on
3rd day roots were developed in plants. On 12th day Vigna radiata
showed more growth in 0.1% HNO3 and 0.1% H2SO4 treated plants
as compare to control plants. On 20th day development of
discoloration of plant pigments were observed on acid treated plants
leaves. On 34th day Vigna radiata showed flower in 0.1% HNO3,
0.01% HNO3 and 0.01% H2SO4treated plants and no flowers were
observed on control plants. On 42th day 0.1% HNO3, 0.01% HNO
and 0.01% H2SO4 treated Vigna radiata variety and control plants
were showed seeds on plants. In Vigna radiate variety 0.1%, 0.01%
HNO3, 0.01% H2SO4treated plants were dead on 46th day and fungal
growth was observed. The toxicological study was carried out on
Vigna radiata plants exposed to 1% HNO3 cells were damaged more
than 1% H2SO4. Leaf sections exposed to 0.001% HNO3 & H2SO4
showed less damaged of cells and pigmentation observed in entire
slide when compare with control plant.
Abstract: Carbon dioxide is one of the major green house gases.
It is removed from different streams using amine absorption process.
Sterically hindered amines are suggested as good CO2 absorbers.
Solubility of carbon dioxide (CO2) was measured in aqueous
solutions of 2-Amino-2-methyl-1-propanol (AMP) at temperatures 30
oC, 40 oC and 60 oC. The effect of pressure and temperature was
studied over various concentrations of AMP. It has been found that
pressure has positive effect on CO2 solubility where as solubility
decreased with increasing temperature. Absorption performance of
AMP increased with increasing pressure. Solubility of aqueous AMP
was compared with mo-ethanolamine (MEA) and the absorption
capacity of aqueous solutions of AMP was found to be better.
Abstract: Boron minerals are very useful for various industrial
activities, such as glass industry and detergent industry, due to its
mechanical and chemical properties. During the production of boron
compounds, many of these are introduced into the environment in the
form of waste. Boron is also an important micro nutrient for the
plants to vegetate but if it exists in high concentrations, it could have
toxic effects. The maximum boron level in drinking water for human
health is given as 0.3 mg/L in World Health Organization (WHO)
standards. The toxic effects of boron should be noted especially for
dry regions, thus, in recent years, increasing attention has been paid
to remove the boron from waste waters. In this study, boron removal
is implemented by ion exchange process using Amberlite IRA-743
resin. Amberlite IRA-743 resin is a boron specific resin and it
belongs to the polymerizate sorbent group within the aminopolyol
functional group. Batch studies were performed to investigate the
effects of various experimental parameters, such as adsorbent dose,
initial concentration and pH, on the removal of boron. It is found
that, when the adsorbent dose increases removal of boron from the
liquid phase increases. However, an increase in the initial
concentration decreases the removal of boron. The effective pH
values for removal of boron are determined between 8.5 and 9.
Equilibrium isotherms were also analyzed by Langmuir and
Freundlich isotherm models. The Langmuir isotherm is obeyed better
than the Freundlich isotherm.
Abstract: This study investigated the removal efficiency of electrokinetic remediation of copper-contaminated soil at different combinations of enhancement reagents used as anolyte and catholyte. Sodium hydroxide (at 0.1, 0.5, and 1.0 M concentrations) and distilled water were used as anolyte, while lactic acid (at 0.01, 0.1, and 0.5 M concentrations), ammonium citrate (also at 0.01, 0.1, and 0.5 M concentrations) and distilled water were used as catholyte. A continuous voltage application (1.0 VDC/cm) was employed for 240 hours for each experiment. The copper content of the catholyte was determined at the end of the 240-hour period. Optimization was carried out with a Response Surface Methodology - Optimal Design, including F test, and multiple comparison method, to determine which pair of anolyte-catholyte was the most significant for the removal efficiency. "1.0 M NaOH" was found to be the most significant anolyte while it was established that lactic acid was the most significant type of catholyte to be used for the most successful electrokinetic experiments. Concentrations of lactic acid should be at the range of 0.1 M to 0.5 M to achieve maximum percent removal values.
Abstract: Due to the low heavy metal concentrations, the
bottom ash from a 32 MW municipal district heating plant was
determined to be a potential forest fertilizer as such. However,
additional Ca would be needed, because its Ca concentration of 1.9-
% (d.w.) was lower than the statutory Finnish minimum limit value
of 6.0-% (d.w.) for Ca in forest fertilizer. Due to the elevated As
concentration (53.0 mg/kg; d.w.) in the fly ash from the 32 MW
municipal district heating plant, and Cr concentration (620 mg/kg;
d.w.) in the ash fraction (i.e. mixture of the bottom ash and fly ash)
from the 6 MW municipal district heating plant, which exceed the
limit values of 30 mg/kg (d.w.) and 300 mg/kg (d.w.) for As and Cr,
respectively, these residues are not suitable as forest fertilizers.
Although these ash fractions cannot be used as a forest fertilizer as
such, they can be used for the landscaping of landfills or in industrial
and other areas that are closed to the public. However, an
environmental permit is then needed.
Abstract: Sugarcane bagasses are one of the most extensively used agricultural residues. Using acid hydrolysis and fermentation, conversion of sugarcane bagasses to lactic acid was technically and economically feasible. This research was concerned with the solubility of lignin in ammonium hydroxide, acid hydrolysis and lactic acid fermentation by Lactococcus lactis, Lactobacillus delbrueckii, Lactobacillus plantarum, and Lactobacillus casei. The lignin extraction results for different ammonium hydroxide concentrations showed that 10 % (v/v) NH4OH was favorable to lignin dissolution. Acid hydrolysis can be enhanced with increasing acid concentration and reaction temperature. The optimum glucose and xylose concentrations occurred at 121 ○C for 1 hour hydrolysis time in 10% sulphuric acid solution were 32 and 11 g/l, respectively. In order to investigate the significance of medium composition on lactic acid production, experiments were undertaken whereby a culture of Lactococcus lactis was grown under various glucose, peptone, yeast extract and xylose concentrations. The optimum medium was composed of 5 g/l glucose, 2.5 g/l xylose, 10 g/l peptone and 5 g/l yeast extract. Lactococcus lactis represents the most efficient for lactic acid production amongst those considered. The lactic acid fermentation by Lactococcus lactis after 72 hours gave the highest yield of 1.4 (g lactic acid per g reducing sugar).
Abstract: A local wastewater treatment plant (WWTP)
experiencing poor nitrification tracked down high level of
surfactants in the plant-s influent and effluent. The aims of this project were to assess the potential inhibitory effect of surfactants on activated sludge processes. The effect of the
presence of TergitolNP-9, TrigetolNP-7, Trigetol15-S-9,
dodecylbenzene sulphonate (SDBS) and sodium dodecyl
sulfate (SDS) on activated sludge oxygen uptake rate (OUR) and nitrification were assessed. The average concentration of non-ionic and anionic
surfactants in the influent to the local WWTP were 7 and 8.7 mg/L, respectively. Removal of 67% to 90% of the non-ionic and 93-99% of the anionic surfactants tested were measured. All surfactants tested showed inhibitory effects both on OUR
and nitrification. SDS incurred the lowest inhibition whereas
SDBS and NP-9 caused severe inhibition to OUR and
Nitrification. Activated sludge flocs sizes slightly decreased
after 3 hours contact with the surfactant present in the test.
The results obtained indicated that high concentrations of
surfactants are likely to have an adverse effect on the
performance of WWTPs utilizing activated sludge processes.
Abstract: To determine if the murine insulinoma, β-TC-6, is a
suitable substitute for primary pancreatic β-cells in the study of β-
cell functional heterogeneity, we used three distinct functional assays
to ascertain the cell line-s response to glucose or a glucose analog.
These assays include: (i) a 2-NBDG uptake assay; (ii) a calcium
influx assay, and; (iii) a quinacrine secretion assay. We show that a
population of β-TC-6 cells endocytoses the glucose analog, 2-
NBDG, at different rates, has non-uniform intracellular calcium ion
concentrations and releases quinacrine at different rates when
challenged with glucose. We also measured the Km for β-TC-6
glucose uptake to be 46.9 mM and the Vm to be 8.36 x 10-5
mmole/million cells/min. These data suggest that β-TC-6 might be
used as an alternative to primary pancreatic β-cells for the study of
glucose-dependent β-cell functional heterogeneity.
Abstract: The toxicity of aqueous extracts of two plants,
Nicotiana tobacum and Eucalyptus globulus were investigated against second instar larvae of Lycoriella auripila, one of the most
important pests of button mushroom, using agar dilution technique.
Seven concentrations of aqueous extracts of both plants were applied
on second instar larvae and their mortality were evaluated after 24, 48 and 72 h. The obtained results revealed that aqueous extracts of N.
tabacum and E. globulus caused 77.55 and 72.5% mortality of larvae
of L. auripila at concentration of 4000 ppm after 72h, respectively. Toxicities of tobacco extract after 24, 48 and 72 h were 1.52, 1.85
and 1.70 times greather than eucalyptus, respectively. The estimated LC50 after 24, 48 and 72 h were 7316.5, 2468.5 and 2013.1 ppm for
tobacco and 64870.0, 6839.5 and 3326.4 ppm for eucalyptus, respectively. These plants merit further study as potential insecticides
for the control of L. auripila.
Abstract: A measurement system was successfully fabricated to
detect ion concentrations (hydrogen and chlorine) in this study.
PIC18F4520, the microcontroller was used as the control unit in the
measurement system. The measurement system was practically used
to sense the H+ and Cl- in different examples, and the pH and pCl
values were exhibited on real-time LCD display promptly. In the study,
the measurement method is used to judge whether the response voltage
is stable. The change quantity is smaller than 0.01%, that the present
response voltage compares with next response voltage for H+
measurement, and the above condition is established only 6 sec.
Besides, the change quantity is smaller than 0.01%, that the present
response voltage compares with next response voltage for Clmeasurement,
and the above condition is established only 5 sec.
Furthermore, the average error quantities would also be considered,
and they are 0.05 and 0.07 for measurements of pH and pCl values,
respectively.
Abstract: the obligatory step during immunoglobulin and lysozyme concentration process is thermal treatment. The combination of temperature and time used in processing can affect the structure of the proteins and involve unfolding and aggregation. The aim of the present study was to evaluate the heat stability of total Igs, the particular immunoglobulin classes and lysozyme in milk. Milk samples were obtained from conventional dairy herd in Latvia. Raw milk samples were pasteurized in different regimes: 63 °C 30 min, 72 °C 15-20 s, 78 °C 15-20 s, 85 °C 15-20 s, 95 °C 15-20 s. The concentrations of Igs (IgA, IgG, IgM) and lysozyme were determined by turbodimetric method. During research was established, that activity of antimicrobial proteins decreases differently. Less concentration reduce was established in a case of lysozyme.
Abstract: There are a many of needs for the development of
SiC-based hydrogen sensor for harsh environment applications. We
fabricated and investigated Pd/Ta2O5/SiC-based hydrogen sensors
with MOS capacitor structure for high temperature process monitoring
and leak detection applications in such automotive, chemical and
petroleum industries as well as direct monitoring of combustion
processes. In this work, we used silicon carbide (SiC) as a substrate to
replace silicon which operating temperatures are limited to below
200°C. Tantalum oxide was investigated as dielectric layer which has
high permeability for hydrogen gas and high dielectric permittivity,
compared with silicon dioxide or silicon nitride. Then, electrical
response properties, such as I-V curve and dependence of capacitance
on hydrogen concentrations were analyzed in the temperature ranges
of room temperature to 500°C for performance evaluation of the
sensor.
Abstract: We present an Electronic Nose (ENose), which is
aimed at identifying the presence of one out of two gases, possibly
detecting the presence of a mixture of the two. Estimation of the
concentrations of the components is also performed for a volatile
organic compound (VOC) constituted by methanol and acetone, for
the ranges 40-400 and 22-220 ppm (parts-per-million), respectively.
Our system contains 8 sensors, 5 of them being gas sensors (of the
class TGS from FIGARO USA, INC., whose sensing element is a tin
dioxide (SnO2) semiconductor), the remaining being a temperature
sensor (LM35 from National Semiconductor Corporation), a
humidity sensor (HIH–3610 from Honeywell), and a pressure sensor
(XFAM from Fujikura Ltd.).
Our integrated hardware–software system uses some machine
learning principles and least square regression principle to identify at
first a new gas sample, or a mixture, and then to estimate the
concentrations. In particular we adopt a training model using the
Support Vector Machine (SVM) approach with linear kernel to teach
the system how discriminate among different gases. Then we apply
another training model using the least square regression, to predict
the concentrations.
The experimental results demonstrate that the proposed
multiclassification and regression scheme is effective in the
identification of the tested VOCs of methanol and acetone with
96.61% correctness. The concentration prediction is obtained with
0.979 and 0.964 correlation coefficient for the predicted versus real
concentrations of methanol and acetone, respectively.