Abstract: Cytogenetic analysis still remains the gold standard method for prenatal diagnosis of trisomy 21 (Down syndrome, DS). Nevertheless, the conventional cytogenetic analysis needs live cultured cells and is too time-consuming for clinical application. In contrast, molecular methods such as FISH, QF-PCR, MLPA and quantitative Real-time PCR are rapid assays with results available in 24h. In the present study, we have successfully used a novel MGB TaqMan probe-based real time PCR assay for rapid diagnosis of trisomy 21 status in Down syndrome samples. We have also compared the results of this molecular method with corresponding results obtained by the cytogenetic analysis. Blood samples obtained from DS patients (n=25) and normal controls (n=20) were tested by quantitative Real-time PCR in parallel to standard G-banding analysis. Genomic DNA was extracted from peripheral blood lymphocytes. A high precision TaqMan probe quantitative Real-time PCR assay was developed to determine the gene dosage of DSCAM (target gene on 21q22.2) relative to PMP22 (reference gene on 17p11.2). The DSCAM/PMP22 ratio was calculated according to the formula; ratio=2 -ΔΔCT. The quantitative Real-time PCR was able to distinguish between trisomy 21 samples and normal controls with the gene ratios of 1.49±0.13 and 1.03±0.04 respectively (p value
Abstract: The performance of high-resolution schemes is investigated for unsteady, inviscid and compressible multiphase flows. An Eulerian diffuse interface approach has been chosen for the simulation of multicomponent flow problems. The reduced fiveequation and seven equation models are used with HLL and HLLC approximation. The authors demonstrated the advantages and disadvantages of both seven equations and five equations models studying their performance with HLL and HLLC algorithms on simple test case. The seven equation model is based on two pressure, two velocity concept of Baer–Nunziato [10], while five equation model is based on the mixture velocity and pressure. The numerical evaluations of two variants of Riemann solvers have been conducted for the classical one-dimensional air-water shock tube and compared with analytical solution for error analysis.
Abstract: Mobile ad hoc network is a collection of mobile
nodes communicating through wireless channels without any existing
network infrastructure or centralized administration. Because of the
limited transmission range of wireless network interfaces, multiple
"hops" may be needed to exchange data across the network. In order
to facilitate communication within the network, a routing protocol is
used to discover routes between nodes. The primary goal of such an
ad hoc network routing protocol is correct and efficient route
establishment between a pair of nodes so that messages may be
delivered in a timely manner. Route construction should be done
with a minimum of overhead and bandwidth consumption. This paper
examines two routing protocols for mobile ad hoc networks– the
Destination Sequenced Distance Vector (DSDV), the table- driven
protocol and the Ad hoc On- Demand Distance Vector routing
(AODV), an On –Demand protocol and evaluates both protocols
based on packet delivery fraction, normalized routing load, average
delay and throughput while varying number of nodes, speed and
pause time.
Abstract: The in vitro culture procedure of purple nutsedge
(Cyperus rotundus L.) for multiple shoot induction and tuber
formation was established. Multiple shoots were significantly
induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige
and Skoog (MS) medium supplemented with 4.44 μM 6-
benzyladinine (BA) alone or in combination with 2.85 μM 1-
indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant
with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of
culturing. Moreover, MS medium supplemented with 4.44 μM BA
and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9
tubers with 3.4 rhizomes per explant. In combination with ancymidol
and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose
or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5
tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively.
However, MS medium containing 3.9 or 7.8 μM ancymidol in
combination with either 60 or 80 g/L sucrose enchanced significant
root formation at 20.9 – 23.6 roots per explant.
Abstract: Flash memory has become an important storage device
in many embedded systems because of its high performance, low
power consumption and shock resistance. Multi-level cell (MLC) is
developed as an effective solution for reducing the cost and increasing
the storage density in recent years. However, most of flash file system
cannot handle the error correction sufficiently. To correct more errors
for MLC, we implement Reed-Solomon (RS) code to YAFFS, what is
widely used for flash-based file system. RS code has longer computing
time but the correcting ability is much higher than that of Hamming
code.
Abstract: This paper is devoted to predict laminar and turbulent
heating rates around blunt re-entry spacecraft at hypersonic
conditions. Heating calculation of a hypersonic body is normally
performed during the critical part of its flight trajectory. The
procedure is of an inverse method, where a shock wave is assumed,
and the body shape that supports this shock, as well as the flowfield
between the shock and body, are calculated. For simplicity the
normal momentum equation is replaced with a second order pressure
relation; this simplification significantly reduces computation time.
The geometries specified in this research, are parabola and ellipsoids
which may have conical after bodies. An excellent agreement is
observed between the results obtained in this paper and those
calculated by others- research. Since this method is much faster than
Navier-Stokes solutions, it can be used in preliminary design,
parametric study of hypersonic vehicles.
Abstract: The aim of the present study was to analyze and
distinguish playing pattern between winning and losing field hockey
team in Delhi 2012 tournament. The playing pattern is focus to the D
penetration (right, center, left.) and to distinguish D penetration
linking to end shot made from it. The data was recorded and analyzed
using Sportscode elite computer software. 12 matches were analyzed
from the tournament. Two groups of performance indicators are used
to analyze, that is D penetration right, center, and left. The type of
shot chosen is hit, push, flick, drag, drag flick, deflect sweep, deflect
push, scoop, sweep, and reverse hit. This is to distinguish the pattern
of play between winning and losing, only 2 performance indicator
showed high significant differences from right (Z=-2.87, p=.004,
p
Abstract: The alternative technique for sterilization of culture
medium to replace autoclaving was carried out. For sterilization of
culture medium without autoclaving, some commercial pure essential
oils, bergamot oil, betel oil, cinnamon oil, lavender oil and turmeric
oil, were tested alone or in combinations with some disinfectants,
10% povidone-iodine and 2% iodine + 2.4% potassium iodide. Each
essential oil or combination was added to 25-mL Murashige and
Skoog (MS) medium before medium was solidified in a 120-mL
container, kept for 2 weeks before evaluating sterile conditions.
Treated media, supplemented with essential oils, were compared to
control medium, autoclaved at 121 degree Celsius for 15 min. In
vitro sterile conditions were found 20 – 100% from these treated
media compared to 100% sterile condition from autoclaved medium.
Treated media obtained 100% sterile conditions were chosen for
culturing chrysanthemum shoots. It was found that 10% povidoneiodine
in combination with cinnamon oil (3:1) and 2% iodine + 2.4%
potassium iodide in combination with lavender oil (1:3) at the
concentration of 36 3L/25 mL medium provided the promising
growth of shoot explants.
Abstract: Cabbage seedlings grown in vitro were exposed to
excess levels of heavy metals, including Cd, Mo, and Zn. High metal
levels affected plant growth at cotyledonary stage. Seedlings under
Cd, Mo, and Zn treatments could not produce root hairs and true
leaves. Under stress conditions, seedlings accumulated a higher
amount of anthocyanins in their cotyledons than those in the control.
The pigments isolated from Cd and Zn stressed seedling cotyledons
appeared as pink, while under Mo stress, was dark pink or purple.
Moreover, excess Mo stress increased antioxidant enzyme activities
of APX, CAT, SOD. These results suggest that, under excess Mo
stress, the induced antioxidant enzyme activity of cabbage seedlings
may function as a protective mechanism to shield the plants from
toxicity and exacerbated growth.
Abstract: Recently, a vehicular ad-hoc networks(VANETs) for
Intelligent Transport System(ITS) have become able safety and convenience services surpassing the simple services such as
an electronic toll collection system. To provide the proper services,
VANET needs infrastructure over the country infrastructure. Thus, we have to spend a huge sum of
human resources. In this reason, several studies have been made on the
usage of cellular networks instead of new protocols
this study is to assess a performance evaluation of the
cellular network for VANET. In this paper, the result of a
for the suitability of cellular networks for VANET
experiment, The LTE(Long Term Evolution) of cellular networks found to be most suitable among the others cellular networks
Abstract: Two species of Physalis, P.angulataL. and P.
peruviana L. were used as models for comparative study to
understand the values of micro-morphological, -anatomical and
architectural characteristics of leaf for taxonomic purposes and
possibly breeding and commercial applications. Both speciespossess
amphistomaticleaves with 1-layer epidermis, 3-4-layer spongy
mesophyll andbicollateral bundle midrib. Palisade parenchyma cells
of P. angulatawere almost twice longer (65-75 μm) than the other
one. Type of stomata was similar as anomocyticbut stomatal
index(SI) at adaxial surface and abaxial surface of P. angulata were
less than of P. peruvianaas 3.57, 4.00 and6.25, 6.66 respectively.
Some leaf architectural characteristics such as leaf shape, order of
venationalsoprovided information of taxonomic significance
Abstract: In a 10-week (May – August, 2008) Phase I trial, 840, 1+ rainbow trout, Oncorhynchus mykiss, received a commercial oral immunomodulator, Fin Immune™, at four different dosages (0, 10, 20 and 30 mg g-1) to evaluate immune response and growth. The overall objective of was to determine an optimal dosage of this product for rainbow trout that provides enhanced immunity with maximal growth and health. Biweekly blood samples were taken from 10 randomly selected fish in each tank (30 samples per treatment) to evaluate the duration of enhanced immunity conferred by Fin-Immune™. The immunological assessment included serum white blood cell (lymphocyte, neutrophil) densities and blood hematocrit (packed cell volume %). Of these three variables, only lymphocyte density increased significantly among trout fed Fin- Immune™ at 20 and 30 mg g-1 which peaked at week 6. At week 7, all trout were switched to regular feed (lacking Fin-Immune™) and by week 10, lymphocyte levels decreased among all levels but were still greater than at week 0. There was growth impairment at the highest dose of Fin-Immune™ tested (30 mg g-1) which can be associated with a physiological compensatory mechanism due to a dose-specific threshold level. Thus, our main objective of this Phase I study was achieved, the 20 mg g-1 dose of Fin-Immune™ should be the most efficacious (of those we tested) to use for a Phase II disease challenge trial.
Abstract: Simulations are developed in this paper with usual DSGE model equations. The model is based on simplified version of Smets-Wouters equations in use at European Central Bank which implies 10 macro-economic variables: consumption, investment, wages, inflation, capital stock, interest rates, production, capital accumulation, labour and credit rate, and allows take into consideration the banking system. Throughout the simulations, this model will be used to evaluate the impact of rate shocks recounting the actions of the European Central Bank during 2008.
Abstract: For decades, the defense business has been plagued by
not having a reliable, deterministic method to know when the Kalman
filter solution for passive ranging application is reliable for use by the
fighter pilot. This has made it hard to accurately assess when the
ranging solution can be used for situation awareness and weapons
use. To date, we have used ad hoc rules-of-thumb to assess when we
think the estimate of the Kalman filter standard deviation on range is
reliable. A reliable algorithm has been developed at BAE Systems
Electronics & Integrated Solutions that monitors the Kalman gain
matrix elements – and a patent is pending. The “settling" of the gain
matrix elements relates directly to when we can assess the time when
the passive ranging solution is within the 10 percent-of-truth value.
The focus of the paper is on surface-based passive ranging – but the
method is applicable to airborne targets as well.
Abstract: Association rules are an important problem in data
mining. Massively increasing volume of data in real life databases
has motivated researchers to design novel and incremental algorithms
for association rules mining. In this paper, we propose an incremental
association rules mining algorithm that integrates shocking
interestingness criterion during the process of building the model. A
new interesting measure called shocking measure is introduced. One
of the main features of the proposed approach is to capture the user
background knowledge, which is monotonically augmented. The
incremental model that reflects the changing data and the user beliefs
is attractive in order to make the over all KDD process more
effective and efficient. We implemented the proposed approach and
experiment it with some public datasets and found the results quite
promising.
Abstract: The paper presents the results of theoretical and
numerical modeling of propagation of shock waves in bubbly liquids
related to nonlinear effects (realistic equation of state, chemical
reactions, two-dimensional effects). On the basis on the Rankine-
Hugoniot equations the problem of determination of parameters of
passing and reflected shock waves in gas-liquid medium for
isothermal, adiabatic and shock compression of the gas component is
solved by using the wide-range equation of state of water in the
analitic form. The phenomenon of shock wave intensification is
investigated in the channel of variable cross section for the
propagation of a shock wave in the liquid filled with bubbles
containing chemically active gases. The results of modeling of the
wave impulse impact on the solid wall covered with bubble layer are
presented.
Abstract: This paper generalizes Yeh Lam-s shock model for
renewal shock arrivals and random threshold. Several interesting
statistical measures are explicitly obtained. A few special cases and
an optimal replacement problem are also discussed.
Abstract: Mobile adhoc network (MANET) is a collection of
mobile devices which form a communication network with no preexisting
wiring or infrastructure. Multiple routing protocols have
been developed for MANETs. As MANETs gain popularity, their
need to support real time applications is growing as well. Such
applications have stringent quality of service (QoS) requirements
such as throughput, end-to-end delay, and energy. Due to dynamic
topology and bandwidth constraint supporting QoS is a challenging
task. QoS aware routing is an important building block for QoS
support. The primary goal of the QoS aware protocol is to determine
the path from source to destination that satisfies the QoS
requirements. This paper proposes a new energy and delay aware
protocol called energy and delay aware TORA (EDTORA) based on
extension of Temporally Ordered Routing Protocol (TORA).Energy
and delay verifications of query packet have been done in each node.
Simulation results show that the proposed protocol has a higher
performance than TORA in terms of network lifetime, packet
delivery ratio and end-to-end delay.
Abstract: Honeycomb sandwich panels are increasingly used in the construction of space vehicles because of their outstanding strength, stiffness and light weight properties. However, the use of honeycomb sandwich plates comes with difficulties in the design process as a result of the large number of design variables involved, including composite material design, shape and geometry. Hence, this work deals with the presentation of an optimal design of hexagonal honeycomb sandwich structures subjected to space environment. The optimization process is performed using a set of algorithms including the gravitational search algorithm (GSA). Numerical results are obtained and presented for a set of algorithms. The results obtained by the GSA algorithm are much better compared to other algorithms used in this study.
Abstract: In this work, we try to find the best setting
of Computational Fluid Dynamic solver available for the problems in
the field of supersonic internal flows. We used the supersonic air-toair
ejector to represent the typical problem in focus. There are
multiple oblique shock waves, shear layers, boundary layers
and normal shock interacting in the supersonic ejector making this
device typical in field of supersonic inner flows. Modeling of shocks
in general is demanding on the physical model of fluid, because
ordinary conservation equation does not conform to real conditions in
the near-shock region as found in many works. From these reasons,
we decided to take special care about solver setting in this article by
means of experimental approach of color Schlieren pictures and
pneumatic measurement. Fast pressure transducers were used to
measure unsteady static pressure in regimes with normal shock in
mixing chamber. Physical behavior of ejector in several regimes is
discussed. Best choice of eddy-viscosity setting is discussed on the
theoretical base. The final verification of the k-ω SST is done on the
base of comparison between experiment and numerical results.