Abstract: Fungal mutant strains have produced cellulase and
xylanase enzymes, and have induced high hydrolysis with enhanced
of rice straw. The mutants were obtained by exposing Penicillium
strain to UV-light treatments. Screening and selection after treatment
with UV-light were carried out using cellulolytic and xylanolytic
clear zones method to select the hypercellulolytic and
hyperxylanolytic mutants. These mutants were evaluated for their
cellulase and xylanase enzyme production as well as their abilities for
biodegradation of rice straw. The mutant 12 UV/1 produced 306.21%
and 209.91% cellulase and xylanase, respectively, as compared with
the original wild type strain. This mutant showed high capacity of
rice straw degradation. The effectiveness of tested mutant strain and
that of wild strain was compared in relation to enhancing the
composting process of rice straw and animal manures mixture. The
results obtained showed that the compost product of inoculated
mixture with mutant strain (12 UV/1) was the best compared to the
wild strain and un-inoculated mixture. Analysis of the composted
materials showed that the characteristics of the produced compost
were close to those of the high quality standard compost. The results
obtained in the present work suggest that the combination between
rice straw and animal manure could be used for enhancing the
composting process of rice straw and particularly when applied with
fungal decomposer accelerating the composting process.
Abstract: The influences of cell-free solutions (CFSs) of lactic
acid bacteria (LAB) on cadaverine and other biogenic amines
production by Listeria monocytogenes and Staphylococcus aureus
were investigated in lysine decarboxylase broth (LDB) using HPLC.
Cell free solutions were prepared from Lactococcus lactis subsp.
lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus
acidilactici and Streptococcus thermophiles. Two different
concentrations that were 50% and 25% CFS and the control without
CFSs were prepared. Significant variations on biogenic amine
production were observed in the presence of L. monocytogenes and S.
aureus (P < 0.05). The function of CFS on biogenic amine production
by foodborne pathogens varied depending on strains and specific
amine. Cadaverine formation by L. monocytogenes and S. aureus in
control were 500.9 and 948.1 mg/L, respectively while the CFSs of
LAB induced 4-fold lower cadaverine production by L.
monocytogenes and 7-fold lower cadaverine production by S. aureus.
The CFSs resulted in strong decreases in cadaverine and putrescine
production by L. monocytogenes and S. aureus, although remarkable
increases were observed for histamine, spermidine, spermine,
serotonin, dopamine, tyramine and agmatine in the presence of LAB
in lysine decarboxylase broth.
Abstract: Mechanical stress has a strong effect on the magnitude
of the Barkhausen-noise in structural steels. Because the
measurements are performed at the surface of the material, for a
sample sheet, the full effect can be described by a biaxial stress field.
The measured Barkhausen-noise is dependent on the orientation of
the exciting magnetic field relative to the axis of the stress tensor.
The sample inhomogenities including the residual stress also
modifies the angular dependence of the measured Barkhausen-noise.
We have developed a laboratory device with a cross like specimen
for bi-axial bending. The measuring head allowed performing
excitations in two orthogonal directions. We could excite the two
directions independently or simultaneously with different amplitudes.
The simultaneous excitation of the two coils could be performed in
phase or with a 90 degree phase shift. In principle this allows to
measure the Barkhausen-noise at an arbitrary direction without
moving the head, or to measure the Barkhausen-noise induced by a
rotating magnetic field if a linear superposition of the two fields can
be assumed.
Abstract: In this investigation, we have evaluated the effects of
arsenic trioxide on hepatic function in pregnant and lactating Swiss
albino mice and their suckling pups. Experiments were carried out on
female mice given 175 ppm As2O3 in their drinking water from the
14th day of pregnancy until day 14 after delivery. Our results showed
a significant decrease in plasma levels of total protein and albumin,
cholesterol and triglyceride in As2O3 treated mice and their pups. The
hyperbilirubinemia and the increased plasma total alkaline
phosphatase activity suggested the presence of cholestasis.
Transaminase activities as well as lactate deshydrogenase activity in
plasma, known as biomarkers of hepatocellular injury, were elevated
indicating hepatic cells’ damage after treatment with As2O3.
Exposure to arsenic led to an increase of liver thiobarbituric acid
reactive substances level along with a concomitant decrease in the
activities of superoxide dismutase, catalase and glutathione
peroxidase and in glutathione.
Abstract: Cell volume, together with membrane potential and
intracellular hydrogen ion concentration, is an essential biophysical
parameter for normal cellular activity. Cell volumes can be altered by
osmotically active compounds and extracellular tonicity.
In this study, a simple mathematical model of osmotically induced
cell swelling and shrinking is presented. Emphasis is given to water
diffusion across the membrane. The mathematical description of the
cellular behavior consists in a system of coupled ordinary differential
equations. We compare experimental data of cell volume alterations
driven by differences in osmotic pressure with mathematical
simulations under hypotonic and hypertonic conditions. Implications
for a future model are also discussed.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: This study was conducted to investigate the effect of
the antioxidant activity of germinated African Yam Bean (AYB) on
oxidative stress markers in alloxan induced diabetic rat. Rats were
randomized into three groups; control, diabetic and germinated AYB
– treated diabetic rats. The Total phenol and flavonoid content and
DPPH radical scavenging activity before and after germination were
investigated. The glucose level, lipid peroxidation and reduced
glutathione of the animals were also determined using standard
technique for four weeks. Germination increased the total phenol,
flavonoid and antioxidant activity of AYB extract by 19.14%,
32.28% and 57.25% respectively. The diabetic rats placed on
germinated AYB diet had a significant decrease in the blood glucose
and lipid peroxidation with a corresponding increase in glutathione
(p
Abstract: In orthopedic surgery there are various situations in
which the surgeon needs to implement methods of cutting and
drilling the bone. With this type of procedure the generated friction
leads to a localized increase in temperature, which may lead to the
bone necrosis. Recognizing the importance of studying this
phenomenon, an experimental evaluation of the temperatures
developed during the procedure of drilling bone has been done.
Additionally the influence of the use of the procedure with / without
additional lubrication during drilling of bone has also been done. The
obtained results are presented and discussed and suggests an
advantage in using additional lubrication as a way to minimize the
appearance of bone tissue necrosis during bone drilling procedures.
Abstract: Chemotherapy and radiotherapy are one of the major
treatment modalities that play important role in the management of a
number of different cancers. This study for the first time evaluates the
toxicity of these treatment modalities and its impact on quality of life
of cancer patients in Pakistan. The study also for the first time
determines what cancer patients of different ages and cancer stages
believe would be an effective intervention to manage their
psychosocial needs and treatment induced toxicity. The article also
provides evidence based approach for the use of variety of
interventions to mange cancer treatment induced morbidity and
toxicity. In light of the present study and reviewed research data,
evidence based recommendations are also made for selection of
appropriate interventions to manage Pain, Nausea and Vomiting,
Anxiety and Depression, Fatigue and Overall QOL of cancer
survivors.
Abstract: Anthocyanins are natural pigments with effective UV
protection but their topical use could be limited due to their
physicochemical characteristics. An attempt to overcome such
limitations by complexation of 2 major anthocyanin-rich sources, C.
ternatea and Z. mays, has potentiated its use as topical antiinflammatory.
Cell studies indicate no cytotoxicity of the
anthocyanin complex (AC) up to 1 mg/ml tested in HaCaT and
human fore head fibroblasts by MTT. Croton oil-induced ear edema
in Wistar rats suggests an effective dose of 5 mg/cm2 of AC as a
topical anti-inflammatory in comparison to 0.5 mg/cm2 of
fluocinolone acetonide. Niosomal encapsulation of the AC
significantly prolonged the anti-inflammatory activity particularly at
8 h after topical application (p = 0.0001). The AC was not cytotoxic
and its anti-inflammatory and activity was dose-dependent and
prolonged by niosomal encapsulation. It has also shown to promote
collagen type 1 production in cell culture. Thus, AC could be a
potential candidate for topical anti-inflammatory agent from natural
resources.
Abstract: Liver disorders are one of the major problems of the
world. Despite its frequent occurrence, high morbidity and high
mortality, its medical management is currently inadequate. This study
was designed to evaluate the hepatoprotective effect of saponin
extract of the root of Garcinia kola on the integrity of the liver of
paracetamol induced wistar albino rats. Twenty five (25) male adult
wistar albino rats were divided into five (5) groups. Group I was the
Control group that received distilled water only, group II was the
negative control that received 2 g/kg of paracetamol on the 13th day,
and group III, IV and V were pre-treated with 100, 200 and
400mg/kg of the saponin extract before inducing the liver damage on
the 13th day with 2 g/kg of paracetamol. Twenty four (24) h after
administration, the rats were sacrificed and blood samples were
collected. The serum Alanine Transaminase (ALT), Aspartate
Transaminase (AST), Alkaline Phosphatase (ALP) activities,
Bilirubin and conjugated bilirubin, glucose and protein
concentrations were evaluated. The liver was fixed immediately in
Formalin and was processed and stained in Haematoxylin and Eosin
(H&E). Administration of saponin extract from the root of Garcinia
kola significantly decreased paracetamol induced elevated enzymes
in the test group. Also histological observations showed that saponin
extract of the root of Garcinia kola exhibited a significant liver
protection against the toxicant as evident by the cells trying to return
to normal. Saponin extract from the root of Garcinia kola indicated a
protection of structural integrity of the hepatocytic cell membrane
and regeneration of the damaged liver.
Abstract: In this paper we describe the Levenvberg-Marquardt
(LM) algorithm for identification and equalization of CDMA
signals received by an antenna array in communication channels.
The synthesis explains the digital separation and equalization of
signals after propagation through multipath generating intersymbol
interference (ISI). Exploiting discrete data transmitted and three
diversities induced at the reception, the problem can be composed
by the Block Component Decomposition (BCD) of a tensor of
order 3 which is a new tensor decomposition generalizing the
PARAFAC decomposition. We optimize the BCD decomposition by
Levenvberg-Marquardt method gives encouraging results compared to
classical alternating least squares algorithm (ALS). In the equalization
part, we use the Minimum Mean Square Error (MMSE) to perform
the presented method. The simulation results using the LM algorithm
are important.
Abstract: Floods play a key role in landform evolution of an
area. This process is likely to alter the topography of the earth’s
surface. The present study area, Kota Bharu is very prone to floods
extends from upstream of Kelantan River near Kemubu to the
downstream area near Kuala Besar. These flood events which occur
every year in the study area exhibit a strong bearing on river
morphological set-up. In the present study, three satellite imageries of
different time periods have been used to manifest the post-flood
landform changes. The pre-processing of the images such as subset,
geometric corrections and atmospheric corrections were carried-out
using ENVI 4.5 followed by the analysis processes. Twenty sets of
cross sections were plotted using software Erdas 9.2, ERDAS and
ArcGis 10 for the all three images. The results show a significant
change in the length of the cross section which suggest that the
geomorphological processes play a key role in carving and shaping
the river banks during the floods.
Abstract: Search engine plays an important role in internet, to
retrieve the relevant documents among the huge number of web
pages. However, it retrieves more number of documents, which are
all relevant to your search topics. To retrieve the most meaningful
documents related to search topics, ranking algorithm is used in
information retrieval technique. One of the issues in data miming is
ranking the retrieved document. In information retrieval the ranking
is one of the practical problems. This paper includes various Page
Ranking algorithms, page segmentation algorithms and compares
those algorithms used for Information Retrieval. Diverse Page Rank
based algorithms like Page Rank (PR), Weighted Page Rank (WPR),
Weight Page Content Rank (WPCR), Hyperlink Induced Topic
Selection (HITS), Distance Rank, Eigen Rumor, Distance Rank Time
Rank, Tag Rank, Relational Based Page Rank and Query Dependent
Ranking algorithms are discussed and compared.
Abstract: This paper reveals the interaction between hydrogen
and surface stress in austenitic stainless steel by X-ray diffraction
stress measurement and thermal desorption analysis before and after
being charged with hydrogen. The surface residual stress was varied
by surface finishing using several disc polishing agents. The obtained
results show that the residual stress near surface had a significant
effect on hydrogen absorption behavior, that is, tensile residual stress
promoted the hydrogen absorption and compressive one did opposite.
Also, hydrogen induced equi-biaxial stress and this stress has a linear
correlation with hydrogen content.
Abstract: The presence of endocrine-disrupting compounds,
such as bisphenol A (BPA), in the environment can cause serious
health problems. However, there are controversial opinions. This
study investigated the reproductive, metabolic, oxidative and
immunologic-disrupting effects of bisphenol A in male rabbits.
Rabbits were divided into five groups. The first four rabbit groups
were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten
weeks. The fifth group was administered corn oil as the vehicle. BPA
significantly decreased serum testosterone, estradiol and the free
androgen index (FAI) and significantly increased sex hormone
binding globulin (SHBG) compared with the placebo group. The
higher doses of BPA showed a significant decrease in follicular
stimulating hormone (FSH) and luteinizing hormone (LH). A
significant increase in blood glucose levels was identified in the BPA
groups. The non-significant difference in insulin levels is a novel
finding. The cumulative testicular toxicity of BPA was clearly
demonstrated by the dose-dependent decrease in absolute testes
weight, primary measures of semen quality and a significant increase
in testicular malonaldehyde (MDA). Moreover, BPA significantly
decreased total antioxidant capacity (TAC) and significantly
increased immunoglobulin G (IgG) at the highest concentration. Our
results suggest that BPA, especially at higher doses, is associated
with many adverse effects on metabolism, oxidative stress, immunity,
sperm quality and markers of androgenic action.
Abstract: The liver is the strongest regenerating organ of the
organism, and even with 2/3 surgically removed, it can regenerate
completely. Hence liver cirrhosis may only develop when the
regenerating system is off.
We present the results of a comparative study of structural and
functional characteristics of rat liver tissue under the conditions of
toxic liver cirrhosis development, induced by carbon tetrachloride,
and its prevention/treatment by natural compounds with antioxidant
and immune stimulating action. Studies were made on Wister rats,
weighing 120~140 g. Grape seeds extracts, separately and in
combination with well-known anticirrhotic drug ursodeoxycholic
acid (Urdoxa), have demonstrated effectiveness in prevention of liver
cirrhosis development and its treatment.
Abstract: The phased-array ultrasound transducer types are
utilities for medical ultrasonography as well as optical imaging.
However, their discontinuity characteristic limits the applications due
to the artifacts contaminated into the reconstructed images. Because
of the effects of the ultrasound pressure field pattern to the echo
ultrasonic waves as well as the optical modulated signal, the side
lobes of the focused ultrasound beam induced by discontinuity of the
phased-array ultrasound transducer might the reason of the artifacts.
In this paper, a simple method in approach of numerical simulation
was used to investigate the limitation of discontinuity of the elements
in phased-array ultrasound transducer and their effects to the
ultrasound pressure field. Take into account the change of ultrasound
pressure field patterns in the conditions of variation of the pitches
between elements of the phased-array ultrasound transducer, the
appropriated parameters for phased-array ultrasound transducer
design were asserted quantitatively.
Abstract: A novel simulation method to determine the
displacements of machine tools due to thermal factors is presented.
The specific characteristic of this method is the employment of
original CAD data from the design process chain, which is
interpreted by an algorithm in terms of geometry-based allocation of
convection and radiation parameters. Furthermore analogous models
relating to the thermal behaviour of machine elements are
automatically implemented, which were gained by extensive
experimental testing with thermography imaging. With this a
transient simulation of the thermal field and in series of the
displacement of the machine tool is possible simultaneously during
the design phase. This method was implemented and is already used
industrially in the design of machining centres in order to improve
the quality of herewith manufactured workpieces.
Abstract: The theoretical approach is developed to describe the
change of drops in the atmosphere of own steam and buffer gas under
irradiation. It is shown that the irradiation influences on size of stable
droplet and on the conditions under which the droplet exists. Under
irradiation the change of drop becomes more complex: the not
monotone and periodical change of size of drop becomes possible.
All possible solutions are represented by means of phase portrait. It is
found all qualitatively different phase portraits as function of critical
parameters: rate generation of clusters and substance density.