Abstract: Cocoa beans (Theobroma cocoa L.) are the main components for chocolate manufacturing. The beans must be correctly fermented at first. Traditional process to perform the first fermentation (lactic fermentation) often consists in confining cacao beans using banana leaves or a fermentation basket, both of them leading to a poor product thermal insulation and to an inability to mix the product. Box fermenter reduces this loss by using a wood with large thickness (e>3cm), but mixing to homogenize the product is still hard to perform. Automatic fermenters are not rentable for most of producers. Heat (T>45°C) and acidity produced during the fermentation by microbiology activity of yeasts and bacteria are enabling the emergence of potential flavor and taste of future chocolate. In this study, a cylindro-rotative fermenter (FCR-V1) has been built and coconut fibers were used in its structure to confine heat. An axis of rotation (360°) has been integrated to facilitate the turning and homogenization of beans in the fermenter. This axis permits to put fermenter in a vertical position during the anaerobic alcoholic phase of fermentation, and horizontally during acetic phase to take advantage of the mid height filling. For circulation of air flow during turning in acetic phase, two woven rattan with grid have been made, one for the top and second for the bottom of the fermenter. In order to reduce air flow during acetic phase, two airtight covers are put on each grid cover. The efficiency of the turning by this kind of rotation, coupled with homogenization of the temperature, caused by the horizontal position in the acetic phase of the fermenter, contribute to having a good proportion of well-fermented beans (83.23%). In addition, beans’pH values ranged between 4.5 and 5.5. These values are ideal for enzymatic activity in the production of the aromatic compounds inside beans. The regularity of mass loss during all fermentation makes it possible to predict the drying surface corresponding to the amount being fermented.
Abstract: Current research is targeting new molecular
mechanisms that underlie non-alcoholic fatty liver disease (NAFLD)
and associated metabolic disorders like non-alcoholic steatohepatitis
(NASH). Forty New Zealand White rabbits have been used and fed a
high protein (HP) and energy diet based on grains and containing
11.76 MJ/kg. Boron added to 3 experimental groups’ drinking waters
(30 mg boron/L) as boron compounds. Biochemical analysis
including boron levels, and nuclear magnetic resonance (NMR) based
metabolomics evaluation, and mRNA expression of peroxisome
proliferator-activated receptor (PPAR) family was performed. LDLcholesterol
concentrations alone were decreased in all the
experimental groups. Boron levels in serum and feces were increased.
Content of acetate was in about 2x higher for anhydrous borax group,
at least 3x higher for boric acid group. PPARα mRNA expression
was significantly decreased in boric acid group. Anhydrous borax
attenuated mRNA levels of PPARγ, which was further suppressed by
boric acid. Boron supplementation decreased the degenerative
alterations in hepatocytes. Except borax group other boron groups did
not have a pronounced change in tubular epithels of kidney. In
conclusion, high protein and energy diet leads hepatocytes’
degenerative changes which can be prevented by boron
supplementation. Boric acid seems to be more effective in this
situation.
Abstract: Kigelia africana (Lam.) Benth. (Bignoniaceae) is a
reputed traditional remedy for various human ailments such as skin
diseases, microbial infections, melanoma, stomach troubles,
metabolic disorders, malaria and general pains. In spite of the fruit
being widely used for purposes related to its antibacterial and
antifungal properties, the chemical constituents associated with the
activity have not been fully identified. To elucidate the active
principles, we evaluated the antimicrobial activity of fruit extracts
and purified fractions against Staphylococcus aureus, Enterococcus
faecalis, Moraxella catarrhalis, Escherichia coli, Candida albicans
and Candida tropicalis. Shade-dried fruits were powdered and
extracted with hydroalcoholic (1:1) mixture by soaking at room
temperature for 72 h. The crude extract was further fractionated by
column chromatography, with successive elution using hexane,
dichloromethane, ethyl acetate, acetone and methanol. The
dichloromethane and ethyl acetate fractions were combined and
subjected to column chromatography to furnish a wax and oil from
the eluates of 20% and 40% ethyl acetate in hexane, respectively. The
GC-MS and GC×GC-MS results revealed that linoleic acid, linolenic
acid, palmitic acid, arachidic acid and stearic acid were the major
constituents in both oil and wax. The crude hydroalcoholic extract
exhibited the strongest activity with MICs of 0.125-0.5 mg/mL,
followed by the ethyl acetate (MICs = 0.125-1.0 mg/mL),
dichloromethane (MICs = 0.250-2.0 mg/mL), hexane (MICs = 0.25-
2.0 mg/mL), acetone (MICs = 0.5-2.0 mg/mL) and methanol (MICs =
1.0-2.0 mg/mL), whereas the wax (MICs = 2.0-4.0 mg/mL) and oil
(MICs = 4.0-8.0 mg/mL) showed poor activity. The study concludes
that synergistic interactions of chemical constituents could be
responsible for the antimicrobial activity of K. africana fruits, which
needs a more holistic approach to understand the mechanism of its
antimicrobial activity.
Abstract: Nonalcoholic fatty liver disease (NAFLD) has
increased in conjunction with obesity. The accuracy of risk factors
for detecting NAFLD in obese adolescents has not undergone a
formal evaluation. The aim of this study was to evaluate predictors of
NAFLD among Egyptian female obese adolescents. The study
included 162 obese female adolescents. All were subjected to
anthropometry, biochemical analysis and abdominal ultrasongraphic
assessment. Metabolic syndrome (MS) was diagnosed according to
the IDF criteria. Significant association between presence of MS and
NAFLD was observed. Obese adolescents with NAFLD had
significantly higher levels of ALT, triglycerides, fasting glucose,
insulin, blood pressure and HOMA-IR, whereas decreased HDL-C
levels as compared with obese cases without NAFLD. Receiver–
operating characteristic (ROC) curve analysis shows that ALT is a
sensitive predictor for NAFLD, confirming that ALT can be used as a
marker of NAFLD.
Abstract: The use of herbs to treat disease is accompanied with the history of human life. This research is aimed to study the anti-inflammatory and antinociceptive effects of hydroalcoholic extract of aerial parts of "Tanacetum balsamita balsamita". In the experimental studies 144 male mice are used. In the inflammatory test, animals were divided into six groups: Control, positive control (receiving Dexamethason at dose of 15mg/kg), and four experimental groups receiving Tanacetum balsamita balsamita hydroalcoholic extract at doses of 25, 50, 100 and 200mg/kg. Xylene was used to induce inflammation. Formalin was used to study the nociceptive effects. Animals were divided into six groups: control group, positive control group (receiving morphine) and four experimental groups receiving Tanacetum balsamita balsamita (Tb.) hydroalcoholic extract at doses of 25, 50, 100 and 200mg/kg. I.p. injection of drugs or normal saline was performed 30 minutes before test. The data were analyzed by using one way Variance analysis and Tukey post test. Aerial parts of Tanacetum balsamita balsamita hydroalcoholic extract decreased significantly inflammatory at dose of 200mg/kg (P
Abstract: In this paper, several improvements are proposed to
previous work of automated classification of alcoholics and nonalcoholics.
In the previous paper, multiplayer-perceptron neural
network classifying energy of gamma band Visual Evoked Potential
(VEP) signals gave the best classification performance using 800
VEP signals from 10 alcoholics and 10 non-alcoholics. Here, the
dataset is extended to include 3560 VEP signals from 102 subjects:
62 alcoholics and 40 non-alcoholics. Three modifications are
introduced to improve the classification performance: i) increasing
the gamma band spectral range by increasing the pass-band width of
the used filter ii) the use of Multiple Signal Classification algorithm
to obtain the power of the dominant frequency in gamma band VEP
signals as features and iii) the use of the simple but effective knearest
neighbour classifier. To validate that these two modifications
do give improved performance, a 10-fold cross validation
classification (CVC) scheme is used. Repeat experiments of the
previously used methodology for the extended dataset are performed
here and improvement from 94.49% to 98.71% in maximum
averaged CVC accuracy is obtained using the modifications. This
latest results show that VEP based classification of alcoholics is
worth exploring further for system development.
Abstract: In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes
management.
Abstract: The studying of enzymatic esterification of carboxylic
acids and higher alcohols was performed by esterase Saccharomyces
cerevisiae in water-organic medium. Investigation of the enzyme
specificity to acetic substrates showed the best result with acetic acid
in esterification reactions with ethanol whereas within other
carboxylic acids the esterification decreased with acids: hexanoic >
pentanoic > butyric > decanoic. In relation to higher alcohols C3-C5,
esterification increased with alcohols propanol < butanol < amylol.
Also it was determined that esterase was more specific to alcohols
with branched chain such as isobutyl alcohol and isoamyl alcohol.
Data obtained may have important practical implications, for
example, for application of yeast esterase in producing various
volatile esters as well as in enzymatic transformation of volatile acids
and toxic fusel alcohols into volatile esters by providing the
production of the high quality alcoholic beverages with redused
content of higher alcohols as well as with improved degustational and
hygienic properties.
Abstract: In this paper, a second order autoregressive (AR)
model is proposed to discriminate alcoholics using single trial
gamma band Visual Evoked Potential (VEP) signals using 3 different
classifiers: Simplified Fuzzy ARTMAP (SFA) neural network (NN),
Multilayer-perceptron-backpropagation (MLP-BP) NN and Linear
Discriminant (LD). Electroencephalogram (EEG) signals were
recorded from alcoholic and control subjects during the presentation
of visuals from Snodgrass and Vanderwart picture set. Single trial
VEP signals were extracted from EEG signals using Elliptic filtering
in the gamma band spectral range. A second order AR model was
used as gamma band VEP exhibits pseudo-periodic behaviour and
second order AR is optimal to represent this behaviour. This
circumvents the requirement of having to use some criteria to choose
the correct order. The averaged discrimination errors of 2.6%, 2.8%
and 11.9% were given by LD, MLP-BP and SFA classifiers. The
high LD discrimination results show the validity of the proposed
method to discriminate between alcoholic subjects.
Abstract: Vinegar or sour wine is a product of alcoholic and
subsequent acetous fermentation of sugary precursors derived from
several fruits or starchy substrates. This delicious food additive and
supplement contains not less than 4 grams of acetic acid in 100 cubic
centimeters at 20°C. Among the large number of bacteria that are
able to produce acetic acid, only few genera are used in vinegar
industry most significant of which are Acetobacter and
Gluconobacter. In this research we isolated and identified an
Acetobacter strain from Iranian apricot, a very delicious and sensitive
summer fruit to decay, we gathered from fruit's stores in Isfahan,
Iran. The main culture media we used were Carr, GYC, Frateur and
an industrial medium for vinegar production. We isolated this strain
using a novel miniature fermentor we made at Pars Yeema
Biotechnologists Co., Isfahan Science and Technology Town (ISTT),
Isfahan, Iran. The microscopic examinations of isolated strain from
Iranian apricot showed gram negative rods to cocobacilli. Their
catalase reaction was positive and oxidase reaction was negative and
could ferment ethanol to acetic acid. Also it showed an acceptable
growth in 5%, 7% and 9% ethanol concentrations at 30°C using
modified Carr media after 24, 48 and 96 hours incubation
respectively. According to its tolerance against high concentrations of
ethanol after four days incubation and its high acetic acid production,
8.53%, after 144 hours, this strain could be considered as a suitable
industrial strain for a production of a new type of vinegar, apricot
vinegar, with a new and delicious taste. In conclusion this is the first
report of isolation and identification of an Acetobacter strain from
Iranian apricot with a very good tolerance against high ethanol
concentrations as well as high acetic acid productivity in an
acceptable incubation period of time industrially. This strain could be
used in vinegar industry to convert apricot spoilage to a beneficiary
product and mentioned characteristics have made it as an amenable
strain in food and agricultural biotechnology.
Abstract: Enzymatic hydrolysis of starch from natural sources
finds potential application in commercial production of alcoholic
beverage and bioethanol. In this study the effect of starch
concentration, temperature, time and enzyme concentration were
studied and optimized for hydrolysis of Potato starch powder (of
mesh 80/120) into glucose syrup by immobilized (using Sodium
arginate) α-amylase using central composite design. The
experimental result on enzymatic hydrolysis of Potato starch was
subjected to multiple linear regression analysis using MINITAB 14
software. Positive linear effect of starch concentration, enzyme
concentration and time was observed on hydrolysis of Potato starch
by α-amylase. The statistical significance of the model was validated
by F-test for analysis of variance (p ≤ 0.01). The optimum value of
starch concentration, enzyme concentration, temperature, time and
were found to be 6% (w/v), 2% (w/v), 40°C and 80min respectively.
The maximum glucose yield at optimum condition was 2.34 mg/mL.
Abstract: There is a variety of inconsistencies in the differences
in alcohol use and related problems between male and female
genders. This study was aimed at analyzing the gender differences in
alcohol use and related problems among university students in
Minsk, Belarus. A total of 465 male (average age of 21) and 1030
female (average age of 20.5) students from four major universities in
Minsk, Belarus were administered WHO recommended standardized
screening instruments – AUDIT, MAST, CAGE questionnaire, as
well as other alcohol related questions. The male to female ratio for
the prevalence of alcohol problems according to the AUDIT was
3.34, while the ratio for alcohol users was 0.97. There are a wide
gender differences in the pattern of alcohol use and preference for
different alcoholic beverages, cause for drinking, and other alcohol
related problems like injuries and blackouts.
Abstract: Titanium oxide films with different morphologies have for the first time been fabricated through hydrothermal reactions between a titanium substrate and iodine powder in water or ethanol. SEM revealed that iodine supported titanium (Ti-I2) surface shows different morphologies with variable treatment conditions. The mean surface roughness (Ra) was increased in the different groups. Use of surfactant has a role to increase the roughness of the film. The surface roughness was in the range of 0.15 μm-0.42 μm. Furthermore, the electrochemical examinations showed that the Ti-I2 surface fabricated in alcoholic medium has high corrosion resistance than in aqueous medium.
Abstract: This is the first report from India on a beverage resulting from alcoholic fermentation of the juice of sea buckthorn (Hippophae rhamnoides L) using lab isolated yeast strain. The health promoting potential of the product was evaluated based on its total phenolic content. The most important finding was that under the present fermentation condition, the total phenolic content of the wine product was 689 mg GAE/L. Investigation of influence of bottle ageing on the sea buckthorn wine showed a slight decrease in the phenolic content (534 m mg GAE/L). This study also includes the comparative analysis of the phenolic content of wines from other selected fruit juices like grape, apple and black currant. KeywordsAlcoholic fermentation, Hippophae, Total phenolic content, Wine
Abstract: Champs Bourcin black grape originated from
Aquitaine, France and planted in Sapa, Lao cai provice, exhibited
high total acidity (11.72 g/L). After 9 days of alcoholic fermentation
at 25oC using Saccharomyces cerevisiae UP3OY5 strain, the ethanol
concentration of wine was 11.5% v/v, however the sharp sour taste of
wine has been found. The malolactic fermentation (MLF) was carried
out by Oenococcus oeni ATCCBAA-1163 strain which had been preadapted
to acid (pH 3-4) and ethanol (8-12%v/v) conditions. We
obtained the highest vivability (83.2%) upon malolactic fermentation
after 5 days at 22oC with early stationary phase O. oeni cells preadapted
to pH 3.5 and 8% v/v ethanol in MRS medium. The malic
acid content in wine was decreased from 5.82 g/L to 0.02 g/L after
MLF (21 days at 22oC). The sensory quality of wine was
significantly improved.
Abstract: The present study was designed to investigate the
cardio protective role of chronic oral administration of alcoholic
extract of Terminalia arjuna in in-vivo ischemic reperfusion injury
and the induction of HSP72. Rabbits, divided into three groups, and
were administered with the alcoholic extract of the bark powder of
Terminalia arjuna (TAAE) by oral gavage [6.75mg/kg: (T1) and
9.75mg/kg: (T2), 6 days /week for 12 weeks]. In open-chest
Ketamine pentobarbitone anaesthetized rabbits, the left anterior
descending coronary artery was occluded for 15 min of ischemia
followed by 60 min of reperfusion. In the vehicle-treated group,
ischemic-reperfusion injury (IRI) was evidenced by depression of
global hemodynamic function (MAP, HR, LVEDP, peak LV (+) & (-
) (dP/dt) along with depletion of HEP compounds. Oxidative stress
in IRI was evidenced by, raised levels of myocardial TBARS and
depletion of endogenous myocardial antioxidants GSH, SOD and
catalase. Western blot analysis showed a single band corresponding
to 72 kDa in homogenates of hearts from rabbits treated with both the
doses. In the alcoholic extract of the bark powder of Terminalia
arjuna treatment groups, both the doses had better recovery of
myocardial hemodynamic function, with significant reduction in
TBARS, and rise in SOD, GSH, catalase were observed. The results
of the present study suggest that the alcoholic extract of the bark
powder of Terminalia arjuna in rabbit induces myocardial HSP 72
and augments myocardial endogenous antioxidants, without causing
any cellular injury and offered better cardioprotection against
oxidative stress associated with myocardial IR injury.
Abstract: In present study the effects of anti-inflammatory and
antinociceptive of vitex hydro-alcoholic extract were evaluated on
male mice. In inflammatory test mice were divided into 7 groups:
first group was control. The second group, positive control group,
received dexamethasone (15 mg/kg) and the other five groups
received different doses of hydroalcohol extract of Vitex fruit (265,
365, 465, 565, and 665 mg/kg). The inflammation was caused by
xylene-induced ear edema. Formalin test was used for evaluation of
antinociceptive effect of extract. In this test, mice were divided into 7
groups: control, morphine (10mg/kg) as positive control group, and
Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs
were administered intrapritoneally, 30 min before each test. The data
were analyzed using one-way ANOVA followed by Tukey-kramer
multiple comparison test. Results have shown significant antiinflammatory
effects of extract at all dosed as compared with control
(P
Abstract: The objective of this research was to study the themes
of alcoholic beverage advertisements in Thailand after the enactment
of the 2008 Alcoholic Beverage Control Act. Data was collected
through textual analysis of 35 television and cinema advertisements
for alcoholic beverage products broadcast in Thailand. Nine themes
were identified, seven of which were themes that had previously been
used before the new law (i.e. power, competition, friendship,
Thainess, success, romance and safety) and two of which were new
themes (volunteerism and conservation) that were introduced as a
form of adaptation and negotiation in response to the new law.
Abstract: According to the governmental data, the cases of oral
cancers doubled in the past 10 years. This had brought heavy burden to
the patients- family, the society, and the country. The literature
generally evidenced the betel nut contained particular chemicals that
can cause oral cancers. Research in Taiwan had also proofed that 90
percent of oral cancer patients had experience of betel nut chewing. It
is thus important to educate the betel-nut hobbyists to cease such a
hazardous behavior. A program was then organized to establish
several training classes across different areas specific to help ceasing
this particular habit. Purpose of this research was to explore the
attitude and intention toward ceasing betel-nut chewing before and
after attending the training classes. 50 samples were taken from a
ceasing class with average age at 45 years old with high school
education (54%). 74% of the respondents were male in service or
agricultural industries. Experiences in betel-nut chewing were 5-20
years with a dose of 1-20 pieces per day. The data had shown that 60%
of the respondents had cigarette smoking habit, and 30% of the
respondents were concurrently alcoholic dependent. Research results
indicated that the attitude, intentions, and the knowledge on oral
cancers were found significant different between before and after
attendance. This provided evidence for the effectiveness of the training
class. However, we do not perform follow-up after the class.
Noteworthy is the test result also shown that participants who were
drivers as occupation, or habitual smokers or alcoholic dependents
would be less willing to quit the betel-nut chewing. The test results
indicated as well that the educational levels and the type of occupation
may have significant impacts on an individual-s decisions in taking
betel-nut or substance abuse.
Abstract: The regulatory competence of blood glucose homeostasis might determine the degree of academic performance. The aim of this study was to produce a model of students' alcohol use based on glucose homeostasis control and cognitive functions that might define the pathogenetic mechanism of alcohol's effect on academic performance. The study took six hours and thirty minutes on fasting, involving thirteen male students. Disturbances in cognitive functions, precisely a decrease in the effectiveness of active attention and a faster development of fatigue after four to six hours of mental work in alcohol users, compared to abstainers was statistically proven. These disturbances in alcohol users were retained even after seven to ten days of moderate alcohol use and might be the reason for the low academic performances among students who use alcoholic beverages.