Abstract: Zymomonas mobilis is known as an example of the
uncoupled growth phenomenon. This microorganism also has a
unique metabolism that degrades glucose by the Entner–Doudoroff
(ED) pathway. In this paper, a genome-scale metabolic model
including 434 genes, 757 reactions and 691 metabolites was
reconstructed to simulate uncoupled growth and study its effect on
flux distribution in the central metabolism. The model properly
predicted that ATPase was activated in experimental growth yields of
Z. mobilis. Flux distribution obtained from model indicates that the
major carbon flux passed through ED pathway that resulted in the
production of ethanol. Small amounts of carbon source were entered
into pentose phosphate pathway and TCA cycle to produce biomass
precursors. Predicted flux distribution was in good agreement with
experimental data. The model results also indicated that Z. mobilis
metabolism is able to produce biomass with maximum growth yield
of 123.7 g (mol glucose)-1 if ATP synthase is coupled with growth
and produces 82 mmol ATP gDCW-1h-1. Coupling the growth and
energy reduced ethanol secretion and changed the flux distribution to
produce biomass precursors.
Abstract: Prediction of perturbations after genetic manipulation
(especially gene knockout) is one of the important challenges in
systems biology. In this paper, a new algorithm is introduced that
integrates microarray data into the metabolic model. The algorithm
was used to study the change in the cell phenotype after knockout of
Gss gene in Escherichia coli BW25113. Algorithm implementation
indicated that gene deletion resulted in more activation of the
metabolic network. Growth yield was more and less regulating gene
were identified for mutant in comparison with the wild-type strain.
Abstract: Effect of Beauveria bassiana and Metarhizium anisopliae var. acridum on the 5th instar nymphs of Schistocerca gregaria was studied in the laboratory. Infection by these both entomopathogenic fungi caused reduction in the hemolymph total protein. The average amounts of total proteins were 2.3, 2.07, 2.09 µg/100 ml of haemolymph in the control and M. anisopliae var. acridum, and B. bassiana based-treatments, respectively. Three types of haemocytes were recognized and identified as prohaemocytes, plasmatocytes and granulocytes. The treatment caused significant reduction in the total haemocyte count and in each haemocyte type on the 9th day after its application.
Abstract: The aim of the present study was to evaluate the blood lipid profile and liver lipid peroxidation in normal rat fed with different concentrations of Acacia senegal and Acacia seyal. Thirty six Sprague Dawley male rats each weighing between 180-200g were randomly divided into two groups. Each group contains eighteen rats and were divided into three groups of 6 rats per group. The rats were fed ad libitum with commercial rat’s feed and tap water containing different concentrations of Acacia senegal and Acacia seyal (3% and 6%) for 4 weeks. The results at 4 weeks showed that there was no significant difference (p≤0.05) in the total cholesterol (TC) and triglycerides (TG) between the control group and treated groups while the results for the high density lipoprotein (HDL-C) showed a significant decrease (P≥0.05) at the 3% and 6% of gum arabic treated groups compared to control group. There was a significant increase (P≥0.05) in low density lipoprotein (LDL-C) with 3% and 6% of gum Arabic (GA) groups compared to the control group. The study indicated that there was no significant (p≤0.05) effect on TC and TG but there was significant effect (P≥0.05) on HDL-C and LDL-C in blood lipid profile of normal rat. The results showed that after 4 weeks of treatment the malondialdehyde (MDA) value in rat fed with 6% of A. seyal group was significantly higher (P≥0.05) than control or other treated groups of A. seyal and A. senegal studied. Thus, the two species of gum arabic did not have beneficial effect on blood lipid profile and lipid peroxidation.
Abstract: The flora of Bozdağ (Konya) and its surroundings
were investigated between 2003 and 2005 years; 700 herbarium
specimens belonging to 482 taxa, 257 genera and 57 families were
collected and identified from the area. The families which have the
most taxa in research area are Asteraceae 67 (14.0%), Fabaceae 60
(12.6%), Lamiaceae 57 (11.9%), Brassicaceae 34 (7.1%), Poaceae
30 (6.3%), Rosaceae 24 (5.0%), Caryophyllaceae 23 (4.8%),
Liliaceae 19 (4.0%), Boraginaceae 17 (3.6%), and Apiaceae 13
(2.7%).
The research area is in the district of Konya and is in the B4
square according to the Grid System. The phytogeographic elements
are represented in the study area as follows; Irano-Turanian 91
(18.9%), Mediterranean 72 (14.9%), Euro-Siberian 21 (4.3%). The
phytogeographic regions of 273 (56.6%) taxa are either multi-regional
or unknown. The number of endemic taxa is 79 (16.3%).
Abstract: The Algeria by its location offers a rich and diverse
vegetation. A large number of aromatic and medicinal plants grow
spontaneously. The interest in these plants has continued to grow in
recent years. Their particular properties due to the essential oil
fraction can be utilized to treat microbial infections. To this end, and
in the context of the valuation of the Algerian flora, we became
interested in the species of the family Lamiaceae which is one of the
most used as a global source of spices. The plant on which we have
based our choice is a species of sage "Salvia officinalis" from the
Isser localized region within the province of Boumerdes. This work
focuses on the study of the antimicrobial activity of essential oil
extracted from the leaves of Salvia officinalis. The extraction is
carried out by essential oil hydrodistillation and reveals a yield of
1.06℅. The study of the antimicrobial activity of the essential oil by
the method of at aromatogramme shown that Gram positive bacteria
are most susceptible (Staphylococcus aureus and Bacillus subtilis)
with a strong inhibition of growth. The yeast Candida albicans
fungus Aspergillus niger and have shown moderately sensitive.
Abstract: Diminished antioxidant defense or increased
production of reactive oxygen species in the biological system can
result in oxidative stress which may lead to various
neurodegenerative diseases including Alzheimer’s disease (AD).
Microglial activation also contributes to the progression of AD by
producing several proinflammatory cytokines, nitric oxide (NO) and
prostaglandin E2 (PGE2). Oxidative stress and inflammation have
been reported to be possible pathophysiological mechanisms
underlying AD. In addition, the cholinergic hypothesis postulates that
memory impairment in patient with AD is also associated with the
deficit of cholinergic function in the brain. Although a number of
drugs have been approved for the treatment of AD, most of these
synthetic drugs have diverse side effects and yield relatively modest
benefits. Marine algae have great potential in pharmaceutical and
biomedical applications as they are valuable sources of bioactive
properties such as anticoagulation, antimicrobial, antioxidative,
anticancer and anti-inflammatory. Hence, this study aimed to provide
an overview of the properties of Malaysian seaweeds (Padina
australis, Sargassum polycystum and Caulerpa racemosa) in
inhibiting oxidative stress, neuroinflammation and cholinesterase
enzymes. These seaweeds significantly exhibited potent DPPH and
moderate superoxide anion radical scavenging ability (P
Abstract: In this study, we are interested in a species of the
family of Asteraceae (Tagetes erecta). This family is considered as a
source of antimicrobial extracts with strong capacity. The extraction
of the flavonoids is carried out by the method of liquid/liquid with the
use of successive solvents. Afterwards, we evaluated the biological
activity of the flavonoids on five pathogenic bacterial stocks such as
Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae,
Pseudomonas aeruginosa and Staphylococcus aureus and two stocks
of yeasts to knowing Candida albicans) and Saccharomyces
cerevisiae, by employing the method of the aromatogramme starting
from a solid disc. The result of the antimicrobial activity shows an
action and a variable degree of sensitivity according to bacterial
stocks tested. It will be noted that the flavonoids have an inhibiting
effect on E. coli, B. subtilis, K. pneumoniae and S. aureus. But a
resistance with respect to the extract by P. aeruginosa, C. albicans
and S. cerevisiae is to be mentioned.
Abstract: A field experiment was carried out at Arab El-
Awammer Research Station, Agric. Res. Center. Assiut Governorate
during summer seasons of 2013 and 2014. The present study assessed
the effect of cowpea with maize intercropping on yield and its
components. The experiment comprised of three treatments (sole
cowpea, sole maize and cowpea-maize intercrop). The experimental
design was a randomized complete block with four replications.
Results indicated that intercropped maize plants with cowpea,
exhibited greater potentiality and resulted in higher values of most of
the studied criteria viz., plant height, number of ears/plant, number of
rows/ear, number of grains/row, grains weight/ear, 100–grain weight
and straw and grain yields. Fresh and dry forage yields of cowpea
were lower in intercropping with maize than sole. Furthermore, the
combined of the two seasons revealed that the total Land Equivalent
Ratio (LER) between cowpea and maize was 1.65. The Aggressivity
(A) maize was 0.45 and cowpea was -0.45. This showed that maize
was the dominant crop, whereas cowpea was the dominated. The
Competitive Ratio (CR) indicated that maize more competitive than
cowpea, maize was 1.75 and cowpea was 0.57. The Actual Yield
Loss (AYL) maize was 0.05 and cowpea was -0.40. The Monetary
Advantage Index (MAI) was 2360.80.
Abstract: This study presents an attempt to evaluate the
antioxidant potential and antimicrobial activity of methanolic extract,
and essential oils prepared from the leaves of sage (Salvia officinalis
L.). The content of polyphenol in the methanolic extracts from the
leaves of Salvia officinalis was determined spectrophotometrically,
calculated as gallic acid and catechin equivalent. The essential oils
and methanol extract were also subjected to screenings for the
evaluation of their antioxidant activities using 2, 2-diphenyl-1-
picrylhydrazyl (DPPH) test. While the plant essential oils showed
only weak antioxidant activities, its methanol extract was
considerably active in DPPH (IC50 = 37.29 μg/ml) test. Appreciable
total polyphenol content (31.25 mg/g) was also detected for the plant
methanol extract as gallic acid equivalent in the Folin–Ciocalteu test.
The plant was also screened for its antimicrobial activity and good to
moderate inhibitions were recorded for its essential oils, and
methanol extracts against most of the tested microorganisms.
The present investigation revealed that this plant had rich source
of antioxidant properties. It is for this reason that sage has found
increasing application in food formulations.
Abstract: Animal fats (camel, sheep, goat, rabbit and chicken)
and vegetable oils (corn, sunflower, palm oil and olive oil) were
substituted with different proportions (1, 5, 10 and 20%) of lard.
Fatty acid composition in TG and 2-MG were determined using
lipase hydrolysis and gas chromatography before and after
adulteration. Results indicated that, genuine lard had a high
proportion (60.97%) of the total palmitic acid at 2-MG. However, it
was 8.70%, 16.40%, 11.38%, 10.57%, 29.97 and 8.97% for camel,
beef, sheep, goat, rabbit and chicken, respectively. It could be noticed
also the position-2-MG is mostly occupied by unsaturated fatty acids
among all tested fats except lard. Vegetable oils (corn, sunflower,
palm oil and olive oil) revealed that the levels of palmitic acid
esterifies at 2-MG position was 6.84, 1.43, 9.86 and 1.70%,
respectively. It could be observed also the studied oils had a higher
level of unsaturated fatty acids in the same position, compared with
animal fats under investigation. Moreover, palmitic acid esterifies at
2-MG and PAEF increased gradually as the substituted levels
increased among all tested fat and oil samples. Statistical analysis
showed that the PAEF correlated well with lard level. The detection
of lard in some commercial processed foods (5 French fries, 4 Butter
fats, 5 processed meat and 6 candy samples) was carried out. Results
revealed that 2 samples of French fries and 4 samples of processed
meat contained lard due to their higher PAEF, while butter fat and
candy were free of lard.
Abstract: Due to the determination of the pollution status of
fresh resources in the Egyptian territorial waters is very important for
public health; this study was carried out to reveal the levels of heavy
metals in the shellfish and their environment and its relation to the
highly developed industrial activities in those areas. A total of 100
shellfish samples from the Rosetta, Edku, El-Maadiya, Abo-Kir and
El-Max coasts [10 crustaceans (shrimp) and 10 mollusks (oysters)]
were randomly collected from each coast. Additionally, 10 samples
from both the water and the sediment were collected from each coast.
Each collected sample was analyzed for cadmium, chromium,
copper, lead and zinc residues using a Perkin Elmer atomic
absorption spectrophotometer (AAS). The results showed that the
levels of heavy metals were higher in the water and sediment from
Abo-Kir. The heavy metal levels decreased successively for the
Rosetta, Edku, El-Maadiya, and El-Max coasts, and the
concentrations of heavy metals, except copper and zinc, in shellfish
exhibited the same pattern. For the concentration of heavy metals in
shellfish tissue, the highest was zinc and the concentrations decreased
successively for copper, lead, chromium and cadmium for all coasts,
except the Abo-Kir coast, where the chromium level was highest and
the other metals decreased successively for zinc, copper, lead and
cadmium. In Rosetta, chromium was higher only in the mollusks,
while the level of this metal was lower in the crustaceans; this trend
was observed at the Edku, El-Maadiya and El-Max coasts as well.
Herein, we discuss the importance of such contamination for public
health and the sources of shellfish contamination with heavy metals.
We suggest measures to minimize and prevent these pollutants in the
aquatic environment and, furthermore, how to protect humans from
excessive intake.
Abstract: The presence of endocrine-disrupting compounds,
such as bisphenol A (BPA), in the environment can cause serious
health problems. However, there are controversial opinions. This
study investigated the reproductive, metabolic, oxidative and
immunologic-disrupting effects of bisphenol A in male rabbits.
Rabbits were divided into five groups. The first four rabbit groups
were administered oral BPA (1, 10, 50, or 100 mg/kg/day) for ten
weeks. The fifth group was administered corn oil as the vehicle. BPA
significantly decreased serum testosterone, estradiol and the free
androgen index (FAI) and significantly increased sex hormone
binding globulin (SHBG) compared with the placebo group. The
higher doses of BPA showed a significant decrease in follicular
stimulating hormone (FSH) and luteinizing hormone (LH). A
significant increase in blood glucose levels was identified in the BPA
groups. The non-significant difference in insulin levels is a novel
finding. The cumulative testicular toxicity of BPA was clearly
demonstrated by the dose-dependent decrease in absolute testes
weight, primary measures of semen quality and a significant increase
in testicular malonaldehyde (MDA). Moreover, BPA significantly
decreased total antioxidant capacity (TAC) and significantly
increased immunoglobulin G (IgG) at the highest concentration. Our
results suggest that BPA, especially at higher doses, is associated
with many adverse effects on metabolism, oxidative stress, immunity,
sperm quality and markers of androgenic action.
Abstract: Curcuma longa L. (Zingiberaceae), commonly known
as turmeric, has a long history of traditional uses for culinary
purposes as a spice and a food colorant. The present study aimed to
document the ethnobotanical knowledge about Curcuma longa, and
to assess the variation in the herbalists’ experience in Northeastern
Algeria. Data were collected using semi-structured questionnaires
and direct interviews with 30 herbalists. Ethnobotanical indices,
including the fidelity level (FL%), the relative frequency citation
(RFC), and use value (UV) were determined by quantitative methods.
Diversity in the level of knowledge was analyzed using univariate,
non-parametric, and multivariate statistical methods. Three main
categories of uses were recorded for C. longa: for food, for medicine,
and for cosmetic purposes. As a medicine, turmeric was used for the
treatment of gastrointestinal, dermatological, and hepatic diseases.
Medicinal and food uses were correlated with both forms of
preparation (rhizome and powder). The age group did not influence
the use. Multivariate analyses showed a significant variation in
traditional knowledge, associated with the use value, origin, quality,
and efficacy of the drug. The findings suggested that the geographical
origin of C. longa affected the use in Algeria.
Abstract: Fritillaria oranensis (Liliaceae) was described in 1874
by pomel from Algeria. Plant samples have been collected from the
mount of Tessala (Sidi-Bel-Abbes). The morphological features of
various organs of the plant are described in detail. In the
morphological part of the study, features of various organs of the
plants such as stem and leaf were determined and illustrated.
Ecological studies provide information about the physical and
chemical structure of soil types in Tessala Mountain. The aim of this
original investigation is to put forth ecological and anatomical
features of these species for the first time, but at the same time given
detailed account of the morphological characteristics of the stem and
leaf of Fritillaria oranensis.
Abstract: Ongoing landscape transformation is one of the major
causes behind disappearance of traditional landscapes, and lead to
species and resource loss. Tree in paddy fields in the northeast of
Thailand is one of those traditional landscapes. Using three different
historical time layers, we acknowledged the severe deforestation and
rapid urbanization happened in the region. Despite the general
thinking of decline in tree density as consequences, the heterogeneous
trend of changes in total tree density in three studied landscapes denied
the hypothesis that number of trees in paddy field depend on the length
of land use practice. On the other hand, due to selection of planting
new trees on levees, existence of trees in paddy field now relies on
their values for human use. Besides, changes in land use and landscape
structure had a significant impact on decision of which tree density
level is considered as suitable for the landscape.
Abstract: The effect of Zn2+, Mg2+, and Ba2+ on Saccharomyces
pastorianus performance was evaluated in this study at independent
and three variable combinations. After 96 h of fermentation, high
wort fermentability (%F) = 29.53 was obtained in medium containing
900:4 ppm Mg2+ + Ba2+. Increased ethanol yield 7.35 %(v/v) and
7.13 %(v/v) were obtained in media containing 900:4 ppm Mg2+ +
Ba2+ and 12:900 ppm Zn2+ + Mg2+. Decrease %F = 22.54 and ethanol
yield 6.18 % (v/v) was obtained in medium containing 12:4 ppm Zn2+
+ Ba2+. In media containing the individual ions, increased %F =
27.94 and 26.03 were recorded for media containing 700 ppm Mg2+
and 2 ppm Ba2+ , with ethanol yield of 7.88% (v/v) and 7.62% (v/v)
respectively. Reduced %F and ethanol yield was observed for 10 ppm
Zn2+ and 4 ppm Ba2+ media. The impact of Ba2+ at 1 and 2 ppm was
significant.
Abstract: This study was conducted to evaluate the manganese
removal from aqueous solution using Banana peels activated carbon
(BPAC). Batch experiments have been carried out to determine the
influence of parameters such as pH, biosorbent dose, initial metal ion
concentrations and contact times on the biosorption process. From
these investigations, a significant increase in percentage removal of
manganese 97.4% is observed at pH value 5.0, biosorbent dose 0.8 g,
initial concentration 20 ppm, temperature 25 ± 2°C, stirring rate 200
rpm and contact time 2h. The equilibrium concentration and the
adsorption capacity at equilibrium of the experimental results were
fitted to the Langmuir and Freundlich isotherm models; the Langmuir
isotherm was found to well represent the measured adsorption data
implying BPAC had heterogeneous surface. A raw groundwater
samples were collected from Baharmos groundwater treatment plant
network at Embaba and Manshiet Elkanater City/District-Giza,
Egypt, for treatment at the best conditions that reached at first phase
by BPAC. The treatment with BPAC could reduce iron and
manganese value of raw groundwater by 91.4% and 97.1%,
respectively and the effect of the treatment process on the
microbiological properties of groundwater sample showed decrease
of total bacterial count either at 22°C or at 37°C to 85.7% and 82.4%,
respectively. Also, BPAC was characterized using SEM and FTIR
spectroscopy.
Abstract: Artemisia species, which are medically beneficial, are
widespread in temperate regions of both Northern and Southern
hemispheres among which Iran is located. About 35 species of
Artemisia are indigenous in Iran among them some are widespread in
all or most provinces, yet some are restricted to some specific
regions. In this review paper, initially, GC-Mass results of some
experiments done in different provinces of Iran are mentioned among
them some compounds are common among species, some others are
mostly restricted to other species; after that, medical advantages
based on some researches on species of this genus are reviewed;
different qualities such as anti-leishmania, anti-bacteria, antiviral as
well as anti-proliferative could be mentioned.
Abstract: Ficus deltoidea from the Moraceae family is a popular
medicinal herb in Malaysia. It possesses strong antioxidant and antiinflammatory
properties. In the present study, the anti-inflammatory
effects of F. deltoidea extract on UVB-irradiated HaCaT
Keratinocytes were investigated. HaCaT Keratinocytes were UVBirradiated
(12.5 mJ/cm3) and were treated with 0.05, 0.08 or 0.1% of
F. deltoidea extract. Cell viability following UVB irradiation was
significantly higher in the groups treated with the F. deltoidea extract
at doses of 0.05, 0.08 or 0.1% than in control group with UVB
irradiation only. Tumor necrosis factor-α (TNF-α), interleukin-1α
(IL-1α), interleukin-6 (IL-6) and cyclooxygenase (COX-2) play
primary roles in the inflammation process upon UV irradiation and
are known to be stimulated by UVB irradiation. Treatment with the
F. deltoidea extract dramatically inhibited the UV-induced TNF-α,
IL-1α, IL-6, and COX-2 expression. These results suggest that the F.
deltoidea extract inhibits the production of pro-inflammatory
cytokines and may be an effective protective agent for the treatment
of skin diseases.