Abstract: The efficacy of the separate mixing of four tropical spicy and medicinal plant products: Dennettia tripetala Baker (pepper fruit), Eugenia aromatica Hook (clove), Piper guineense (Schum and Thonn) (black pepper) and Monodora myristica (Dunal) (African nut-meg) with a household vegetable oil was evaluated under tropical storage conditions for the control and reproductive performance of Dermestes maculatus (De Geer) (hide beetle) and Necroba rufipes (De Geer) (copra beetle) on African catfish, Clarias gariepinus (Burchell). Each of the plant materials was pulverized into powder and applied as a mix of 1ml of oil and plant powder at 2.5, 5.0, 7.5 and 10.0g per 100g of dried fish, and allowed to dry for 6h. Each of the four oil-mixed powder treatments evoked significant (P < 05) mortalities of the two insects compared with the control (oil only) at 1, 3 and 7 days post treatment. The oil-powder mixture dosages did not prevent insect egg hatchability but while the emergent larvae on the treated samples died, the emergent larvae in the control survived into adults. The application of oil-mixed powders effectively suppressed the emergence of the larvae of the beetles. Similarly, each of the oil-powder mixtures significantly reduced weight loss in smoked fish that were exposed to D. maculatus and N. rufipes when compared to the control (P < 05). The results of this study suggest that the plant powders rather than the domestic oil demonstrated protective ability against the fish beetles and confirm the efficacy of the plant products as pest control agents.
Abstract: Discovering new biological knowledge from the highthroughput biological data is a major challenge to bioinformatics today. To address this challenge, we developed a new approach for protein classification. Proteins that are evolutionarily- and thereby functionally- related are said to belong to the same classification. Identifying protein classification is of fundamental importance to document the diversity of the known protein universe. It also provides a means to determine the functional roles of newly discovered protein sequences. Our goal is to predict the functional classification of novel protein sequences based on a set of features extracted from each protein sequence. The proposed technique used datasets extracted from the Structural Classification of Proteins (SCOP) database. A set of spectral domain features based on Fast Fourier Transform (FFT) is used. The proposed classifier uses multilayer back propagation (MLBP) neural network for protein classification. The maximum classification accuracy is about 91% when applying the classifier to the full four levels of the SCOP database. However, it reaches a maximum of 96% when limiting the classification to the family level. The classification results reveal that spectral domain contains information that can be used for classification with high accuracy. In addition, the results emphasize that sequence similarity measures are of great importance especially at the family level.
Abstract: The use of magnetic and magnetic/gold core/shell
nanoparticles in biotechnology or medicine has shown good promise
due to their hybrid nature which possesses superior magnetic and
optical properties. Some of these potential applications include
hyperthermia treatment, bio-separations, diagnostics, drug delivery
and toxin removal. Synthesis refinement to control geometric and
magnetic/optical properties, and finding functional surfactants for
biomolecular attachment, are requirements to meet application
specifics.
Various high-temperature preparative methods were used for the
synthesis of iron oxide and gold-coated iron oxide nanoparticles.
Different surface functionalities, such as 11-aminoundecanoic and
11-mercaptoundecanoic acid, were introduced on the surface of the
particles to facilitate further attachment of biomolecular functionality
and drug-like molecules. Nanoparticle thermal stability, composition,
state of aggregation, size and morphology were investigated and the
results from techniques such as Fourier Transform-Infra Red
spectroscopy (FT-IR), Ultraviolet visible spectroscopy (UV-vis),
Transmission Electron Microscopy (TEM) and thermal analysis are
discussed.
Abstract: Prickly pear juice has received renewed attention with regard to the effects of processing and preservation on its sensory qualities (colour, taste, flavour, aroma, astringency, visual browning and overall acceptability). Juice was prepared by homogenizing fruit and treating the pulp with pectinase (Aspergillus niger). Juice treatments applied were sugar addition, acidification, heat-treatment, refrigeration, and freezing and thawing. Prickly pear pulp and juice had unique properties (low pH 3.88, soluble solids 3.68 oBrix and high titratable acidity 0.47). Sensory profiling and descriptive analyses revealed that non-treated juice had a bitter taste with high astringency whereas treated prickly pear was significantly sweeter. All treated juices had a good sensory acceptance with values approximating or exceeding 7. Regression analysis of the consumer sensory attributes for non-treated prickly pear juice indicated an overwhelming rejection, while treated prickly pear juice received overall acceptability. Thus, educed favourable sensory responses and may have positive implications for consumer acceptability.
Abstract: In the other to Study of drought stress and Selenium
spraying effect on superoxide dismotase (SOD) activity of rapeseed
(Brassica napus L.) cultivars in Shahr-e-Rey region, an experiment
carried out in Split factorial design in the basis of randomized
complete blocks with 4 replications in 2006. Irrigation in two levels:
Normal irrigation and irrigation with drought stress when the soil
electrical conductivity reached to 60 as main factor and rapeseed
cultivars in 3 levels Zarfam, Okapi, Opera and selenium spraying at
the beginning of flowering stage in 3 levels: 0, 16 and 21 g/ha as sub
factor.
The results showed that the simple and interaction effect of
irrigation, selenium and cultivars on SOD activity had significant
difference. In this case Zarfam cultivar with 2010 u.mg-1 protein and
Opera with 1454 u.mg-1 protein produced maximum and minimum
amounts of SOD activitiy. Interaction effect of irrigation and variety
showed that, normal irrigation in Opera with 1115 u.mg-1 protein
and drought stress in Zarfam with 2784 u.mg-1 protein conducted to
and minimum and maximum amounts of SOD activity.
Interaction effect of irrigation, cultivar and selenium on SOD
indicated that drought stress condition and 21 gr/ha selenium
spraying in Zarfam variety with 3146 u.mg-1 protein gained to
highest activities of SOD.
Abstract: Microparticles carrier systems made from naturally occurring polymers based on chitosan/casein system appears to be a promising carrier for the sustained release of orally and parenteral administered drugs. In the current study we followed a microencapsulation technique based aqueous coacervation method to prepare chitosan/casein microparticles of compositions 1:1, 1:2 and 1:5 incorporated with chloramphenicol. Glutaraldehyde was used as a chemical cross-linking agent. The microparticles were prepared by aerosol method and studied by optical microscopy, infrared spectroscopy, thermo gravimetric analysis, swelling studies and drug release studies at various pH. The percentage swelling of the polymers are found to be in the order pH 4 > pH 10 > pH 7 and the increase in casein composition decrease the swelling percentage. The drug release studies also follow the above order.
Abstract: The proximate composition, physical traits and
sensory properties of beef and chicken patties incorporated with
various level of dried cornsilk (Maydis stigma) were studied. The
beef and chicken patties were formulated with either 2%, 4% or 6%
of cornsilk. Both cooked beef and chicken patties incorporated with
6% cornsilk recorded the highest protein concentration at 23.3% and
28.42%, respectively. Both cooked beef and chicken patties
containing 6% cornsilk significantly recorded the lowest
concentration of fat at 11.4% and 14.60%, respectively. Beef and
chicken patties formulated with 6% cornsilk recorded the highest
cooking yield at 80.13% and 83.03% compared to other treatments.
The inclusion of cornsilk did not change the sensory properties and
consumer acceptability of cornsilk-based beef and chicken patties.
Cornsilk fibre has been effective in improving cooking yield,
moisture and fat retention of beef and chicken patties
Abstract: This study was conducted to investigate the efficacy
of five herbicides on narrow leaved weeds and growth and yield of
wheat. An experiment was conducted at Agronomic Research
Farm, University of Agriculture Faisalabad. The experiment was
laid out in randomized complete block designee (RCBD) with three
replications. Treatments studied were clodinafop (Topic-15 WG) at
37 g a.i. ha-1, clodinafop (Topaz-15 WG) at 45 g a.i. ha-1,
fenoxaprop-p-ethyl (Puma Super-75 EW) at 45 g a.i. ha-1,
fenoxaprop-p-ethyl (Gramicide-6.9 EW) at 85 g a.i. ha-1,
fenoxaprop-p-ethyl (Chinlima-6.9 EW) at 85 g a.i. ha-1 and weedy
check. Plots treated with fenoxaprop-p-ethyl (Puma Super-75 EW)
at 45 g a.i. ha-1 produced relatively less weed biomass, more plant
height, number of spike bearing tillers, number of grains per spike,
1000-grain weight and grain yield (4.20 t ha-1).
Abstract: MinC plays an important role in bacterial cell division
system by inhibiting FtsZ assembly. However, the molecular
mechanism of the action is poorly understood. E. coli MinC Nterminus
domain was purified and crystallized using 1.4 M sodium
citrate pH 6.5 as a precipitant. X-ray diffraction data was collected
and processed to 2.3 Å from a native crystal. The crystal belonged to
space group P212121, with the unit cell parameters a = 52.7, b = 54.0,
c = 64.7 Å. Assuming the presence of two molecules in the
asymmetric unit, the Matthews coefficient value is 1.94 Å3 Da-1,
which corresponds to a solvent content of 36.5%. The overall
structure of MinCN is observed as a dimer form through anti-parallel
ß-strand interaction.
Abstract: Perspective of food security in 21 century showed
shortage of food that production is faced to vital problem. Food
security strategy is applied longtime method to assess required food.
Meanwhile, nanotechnology revolution changes the world face.
Nanotechnology is adequate method utilize of its characteristics to
decrease environmental problems and possible further access to food
for small farmers. This article will show impact of production and
adoption of nanocrops on food security. Population is researchers of
agricultural research center of Esfahan province. The results of study
show that there was a relationship between uses, conversion,
distribution, and production of nanocrops, operative human
resources, operative circumstance, and constrains of usage of
nanocrops and food security. Multivariate regression analysis by
enter model shows that operative circumstance, use, production and
constrains of usage of nanocrops had positive impact on food security
and they determine in four steps 20 percent of it.
Abstract: In this study, the possibility of using potato as a
substrate for glutamic acid production by Brevibacterium linens
was investigated. For preparation of fermentation medium, potato
was hydrolyzed by hydrochloridric acid. The medium contained
potato hydrolysate, tween 80, mineral solution, glucose, and
potassium hydrogen phosphate. The initial pH of the medium was
adjusted to 7-7.5. For achieving the optimum time with maximum
yield, the beakers containing the medium and the inoculums were
incubated in a rotary water bath flask shaker for one to five days.
Thin layer choromatography was used for quantitative and
qualitative assay of the glutamic acid produced. The results
revealed that as fermentation time increased, pH of the
fermentation medium significantly decreased (P
Abstract: In large Internet backbones, Service Providers
typically have to explicitly manage the traffic flows in order to
optimize the use of network resources. This process is often referred
to as Traffic Engineering (TE). Common objectives of traffic
engineering include balance traffic distribution across the network
and avoiding congestion hot spots. Raj P H and SVK Raja designed
the Bayesian network approach to identify congestion hors pots in
MPLS. In this approach for every node in the network the
Conditional Probability Distribution (CPD) is specified. Based on
the CPD the congestion hot spots are identified. Then the traffic can
be distributed so that no link in the network is either over utilized or
under utilized. Although the Bayesian network approach has been
implemented in operational networks, it has a number of well known
scaling issues.
This paper proposes a new approach, which we call the Pragati
(means Progress) Node Popularity (PNP) approach to identify the
congestion hot spots with the network topology alone. In the new
Pragati Node Popularity approach, IP routing runs natively over the
physical topology rather than depending on the CPD of each node as
in Bayesian network. We first illustrate our approach with a simple
network, then present a formal analysis of the Pragati Node
Popularity approach. Our PNP approach shows that for any given
network of Bayesian approach, it exactly identifies the same result
with minimum efforts. We further extend the result to a more
generic one: for any network topology and even though the network
is loopy. A theoretical insight of our result is that the optimal routing
is always shortest path routing with respect to some considerations of
hot spots in the networks.
Abstract: An experiment was conducted with 80 unsexed
broilers of the Arbor Acress strain to determine the capability of a
carrot and fruit juice wastes mixture (carrot, apple, manggo, avocado,
orange, melon and Dutch egg plant) in the same proportion for
replacing corn in broiler diet. This study involved a completely
randomized design (CRD) with 5 treatments (0, 5, 10, 15, and 20% of
juice wastes mixture in diets) and 4 replicates per treatment. Diets
were isonitrogenous (22% crude protein) and isocaloric (3000 kcal/kg
diet). Measured variables were feed consumption, average daily
gain, feed conversion, as well as percentages of abdominal fat pad,
carcass, digestive organs (liver, pancreas and gizzard), and heart.
Data were analyzed by analysis of variance for CRD. Increasing
juice wastes mixture levels in diets increased feed consumption
(P
Abstract: This study was carried out to evaluate concentration
of micro minerals (Zn, Fe, Mn, Cu and Se) of forages and their
distribution in fiber fraction (neutral detergent fiber/NDF and acid
detergent fiber/ADF) in South Sumatra during dry and rainy seasons.
Seven species of commonly forages namely Axonopus compressus,
Panicum maximum, Pennisetum purpuphoides, Leucaena
leucocephala, Centrocema pubescens, Calopogonium mucunoides
and Acacia mangium were collected at native pasture during rainy
and dry seasons. The results showed that micro minerals
concentration of forages and their distribution in fiber fraction varied
among species and season. In general, concentration of micro
minerals was slightly higher in rainy season compared to dry season
either in grass or legumes forages. In grass, concentration of Fe and
Mn were above the critical level, while 33.3 %, 100 % and 16.7 % of
evaluated grass were deficient in Zn, Cu and Se. Data on legume
forages show that 75 % of legumes were deficient in Zn and Mn, 62.5
% deficient in Cu and 50 % deficient in Se. There was no species of
legume deficient in Fe. Distribution of micro minerals in NDF and
ADF were also significantly affected by species and season and
depends on the kinds of element measured. Generally, micro minerals
were associated in fiber fractions much higher during dry season
compared to rainy season. Iron (Fe) and selenium (Se) in forages
were the highest elements associated in NDF and ADF, while the
lowest was found in Copper (Cu).
Abstract: A feeding trial was conducted to investigate the effect
of periodically use of garlic on performance and carcass
characteristics in broiler chickens. 240 1-day-old Ross broiler chicks
randomly allocated into the 10 dietary treatments (A, B, C, D, E, F,
G, H, I and J) for 6 wk. Treatment A or control group, received basal
diet (based on standards of Ross management guidelines) without
supplementation of garlic powder while B, C and D dietary
treatments were basal diet supplemented with 0.5, 1 and 3% garlic
powder, respectively for the whole time of experiment (6 weeks).
Birds in group E, F and G were fed control diet supplemented with
0.5, 1 and 3% garlic powder, respectively just in their starter diet (0-
21d). Birds in three other treatments (H, I and J) received control diet
for the first 21 days and 0.5, 1 and 3% of garlic powder was added to
their finisher diets, respectively. 1 and 3% supplemented groups in
finisher period had better performance as compared with other
groups. Since present study conducted in optimum and antiseptic
conditions, it seems that better or more responses could be expected
in performance if the raising conditions would not be healthy.
Abstract: Antimicrobial resistant is becoming a major factor in
virtually all hospital acquired infection may soon untreatable is a
serious public health problem. These concerns have led to major
research effort to discover alternative strategies for the treatment of
bacterial infection. Nanobiotehnology is an upcoming and fast
developing field with potential application for human welfare. An
important area of nanotechnology for development of reliable and
environmental friendly process for synthesis of nanoscale particles
through biological systems In the present studies are reported on the
use of fungal strain Aspergillus species for the extracellular synthesis
of bionanoparticles from 1 mM silver nitrate (AgNO3) solution. The
report would be focused on the synthesis of metallic bionanoparticles
of silver using a reduction of aqueous Ag+ ion with the
culture supernatants of Microorganisms. The bio-reduction of the
Ag+ ions in the solution would be monitored in the aqueous
component and the spectrum of the solution would measure through
UV-visible spectrophotometer The bionanoscale particles were
further characterized by Atomic Force Microscopy (AFM), Fourier
Transform Infrared Spectroscopy (FTIR) and Thin layer
chromatography. The synthesized bionanoscale particle showed a
maximum absorption at 385 nm in the visible region. Atomic Force
Microscopy investigation of silver bionanoparticles identified that
they ranged in the size of 250 nm - 680 nm; the work analyzed the
antimicrobial efficacy of the silver bionanoparticles against various
multi drug resistant clinical isolates. The present Study would be
emphasizing on the applicability to synthesize the metallic
nanostructures and to understand the biochemical and molecular
mechanism of nanoparticles formation by the cell filtrate in order to
achieve better control over size and polydispersity of the
nanoparticles. This would help to develop nanomedicine against
various multi drug resistant human pathogens.
Abstract: Levan, an exopolysaccharide, was produced by
Microbacterium laevaniformans and its yield was characterized as a
function of concentrations of date syrup, sucrose and the fermentation
time. The optimum condition for levan production from sucrose was
at concentration of 20% sucrose for 48 h and for date syrup was 25%
for 48 h. The results show that an increase in fermentation time
caused a decrease in the levan production at all concentrations of date
syrup tested. Under these conditions after 48 h in sucrose medium,
levan production reached 48.9 g/L and for date syrup reached 10.48
g/L . The effect of pH on the yield of the purified levan was examined
and the optimum pH for levan production was determined to be 6.0.
Levan was composed mainly of fructose residues when analyzed by
TLC and FT-IR spectroscopy. Date syrup is a cheap substrate widely
available in Iran and has potential for levan production. The thermal
stability of levan was assessed by Thermo Gravimetric Analysis
(TGA) that revealed the onset of decomposition near to 49°C for the
levan produced from sucrose and 51°C for the levan from date syrup.
DSC results showed a single Tg at 98°C for levan produced from
sucrose and 206 °C for levan from date syrup.
Abstract: The effect of wheat flour extraction rates on flour
composition, farinographic characteristics and the quality of
sourdough naans was investigated. The results indicated that by
increasing the extraction rate, the amount of protein, fiber, fat and
ash increased, whereas moisture content decreased. Farinographic
characteristic like water absorption and dough development time
increased with an increase in flour extraction rate but the dough
stabilities and tolerance indices were reduced with an increase in
flour extraction rates. Titratable acidity for both sourdough and
sourdough naans also increased along with flour extraction rate. The
study showed that overall quality of sourdough naans were affected
by both flour extraction rate and starter culture used. Sensory
analysis of sourdough naans revealed that desirable extraction rate
for sourdough naan was 76%.
Abstract: Stocking density is considered one of the important
factors affecting fish growth. But, information related to impact of
stocking density on growth performance of monosex tilapia population
under the ecological conditions of Gangetic plains in West Bengal,
India is limited. The aim of our study was to compare the growth
potential of monosex tilapia at various stocking densities and to
determine an ideal stocking density for culture of all-male monosex
fish. The males were isolated by examination of genital papilla region
and were stocked separately in 0.01 ha earthen ponds at different
stocking densities (5000, 10000, 15000, 20000, 25000 and 30000
fingerlings/ha). It was found that the highest weight, length, daily
weight gain, growth rate and protein content were observed for the
20000 fish/ha density class. Thus, culture of monosex tilapia at a
density of 20000 fish/ha can be considered ideal for augmented
production of the fish under Indian context.
Abstract: The heterotrophic seedling growth can be defined as a
product of two components: (1) the weight of mobilized seed reserve,
and (2) conversion efficiency of utilized seed reserve to seedling
tissue. The first component can be further divided into (1) initial seed
weight, and (2) the fraction of seed reserve, which is mobilized. The
objective of this study was the identification of the sensitive seedling
growth component(s) in response to drought and salinity stresses.
Two experiments were separately conducted using various salinity
levels (osmotic pressure) of 0, 0.25, 0.50, 0.75, 1, 1.25 and 1.5 MPa
created using NaCl as first experiment and by polyethylene glycol
(drought stress) of 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2 and 1.4 MPa in second
experiment. Seeds of five crops species (Hordeum vulgare, Brassica
napus, Zea mays, Medicago sativa and Medicago scutellata) were
used in each experiment. In both experiments, seedling growth,
fraction of seed reserve utilization and weight of mobilized seed
reserve decreased with increasing drought and salt intensity.
However, drought and salinity stresses had no effect on the
conversion efficiency. It was concluded that the sensitive component
of seedling growth is the weight of mobilized seed reserve.