Effect of Drought Stress and Selenium Spraying on Superoxide Dismotase Activity of Winter Rapeseed (Brassica napus L.) Cultivars
In the other to Study of drought stress and Selenium
spraying effect on superoxide dismotase (SOD) activity of rapeseed
(Brassica napus L.) cultivars in Shahr-e-Rey region, an experiment
carried out in Split factorial design in the basis of randomized
complete blocks with 4 replications in 2006. Irrigation in two levels:
Normal irrigation and irrigation with drought stress when the soil
electrical conductivity reached to 60 as main factor and rapeseed
cultivars in 3 levels Zarfam, Okapi, Opera and selenium spraying at
the beginning of flowering stage in 3 levels: 0, 16 and 21 g/ha as sub
factor.
The results showed that the simple and interaction effect of
irrigation, selenium and cultivars on SOD activity had significant
difference. In this case Zarfam cultivar with 2010 u.mg-1 protein and
Opera with 1454 u.mg-1 protein produced maximum and minimum
amounts of SOD activitiy. Interaction effect of irrigation and variety
showed that, normal irrigation in Opera with 1115 u.mg-1 protein
and drought stress in Zarfam with 2784 u.mg-1 protein conducted to
and minimum and maximum amounts of SOD activity.
Interaction effect of irrigation, cultivar and selenium on SOD
indicated that drought stress condition and 21 gr/ha selenium
spraying in Zarfam variety with 3146 u.mg-1 protein gained to
highest activities of SOD.
[1] R.L. Foot, Comparative Responses of Field Grown Crops to Phosphate
Concentrations in Soil Solutions. In: Stress Physiology in Crop Plants,
Mussell, H. and R.C. Stapley (Eds.).Wiley,NewYork,2001,pp.81-
106.URL: www.publish.csiro. au/?act=view_file&file_id=AR9760479.
[2] C.H Foyer, M. Lelandais and K.J. Kunert, Photooxidative stress in
plants. Physiol. Plant,1992,vol 92 ,pp.696-717.
http://cat.inist.fr/?aModele=afficheN&cpsidt=3339390.
[3] M.Germ , I. Kreft I, and J. Osvald, Influence of UV-B exclusion and
selenium treatment on photochemical efficiency of photosystem II, yield
and respiratory potential in pumpkins (Cucurbita pepo L.). Plant Physiol.
Biochem,2005,vol. 43,pp. 445-448.
[4] H.Hartikainen , T. Xue, and V.Piironen, Selenium as an antioxidant and
pro-oxidant in ryegrass. Plant Soil,2000, vol .225,pp. 193-200.
[5] A. Hegedus, S. Erdei, and G. Horvath, Comparative studies of H2O2
detoxifying enzymes in green and greening barley seedlings under
cadmium stress. Plant Sci,2001, vol. 160: 1085-1093. DOI:
10.1016/S0168-9452(01)00330-2.
[6] S.M. Johnson, S.J. Doherty, and R.R.D. Croy, Biphasic superoxide
generation in potato Tubers: A self amplifying response to stress. Plant
Physiol,2003, vol 13,pp. 1440-1449.
http://www.plantphysiol.org/cgi/content/abstract/1 31/3/1440.
[7] V.V. Kuznetsov , V.P. Kholodova , V.V. Kuznetsov B.A. Yagodin ,
Selenium regulates the water status of plants exposed to drought. Dokl.
Biol. Sci,2003,vol.390,pp.266-268.
[8] J.Mwanamwenge, S.P. Loss, K.H.M. Siddique, and P.S. Cocks, Effect of
water stress during floral initiation, flowering and podding on the growth
and yield of faba bean (Vicia faba L.). Eur. J. Agron, 1999,vol. 11,pp.1-
11. DOI: 10.1016/S1161- 0301(99)00003-9.
[9] M.Nasri, M.Khalatbari, H.Zahedi, F.Paknejad, and H.R. Tohidi
Moghadam, Evaluation of Micro and Macro Elements in Drought Stress
Condition in Cultivars of Rapeseed (Brassica napus L.). American
Journal of Agricultural and Biological Sciences, 2003,vol.3 (3),pp.579-
583.
[10] G.Noctor, and C.H. Foyer, Ascorbate and glutathione: Keeping active
oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol,1998,
vol.49,pp 249-279. PMID: 15012235.
[11] J,Nowak, K.Kaklewski, M.Ligocki , Influence of selenium on
oxidoreductive enzymes activity in soil and in plants. Soil Biol.
Biochem,2004,vol. 36,pp.1553-1558.
[12] S.Ouchi, A.Nishikawa, and E.Kameda, Soilimproving effect of a superaterabsorbentpolymer
II. Evaporation, leaching of salts and growth of
vegetables. Jap. J. Soil Sci. Plant Nutr,1990,Vol.61,pp.606-613.
http://www.fao.org/agris/search/display.do?f=./199
4/v2007/JP9306919.xml;JP9306919
[13] A.Pennanen, T.Xue, H.Hartikainen , Protective role of selenium in plant
subjected to severe UV irridiation stress. J. Appl. Bot,2002,vol.76,pp.
66-76.
[14] J.G. Scandalios, Oxygen stress and superoxide dismutase. Plant
Physiol,1993,vol.101,pp.712-726.
http://www.pubmedcentral.nih.gov/articlerender.fc gi?artid=158641.
[15] M.Seppanen , M. Turakainen, H.Hartikainen, Selenium effects on
oxidative stress in potato . Plant Science,2003,vol. 165 ,pp.311-/319.
[16] A.Shalata, V. Mittova, M. Volokita, M. Guy, and M. Tal, Response of
the cultivated tomato and its wild salt-tolerant relative Lycopersicon
pennellii to salt-dependent oxidative stress: The root antioxidative
system. Physiol. Plant,2001,vol. 112: 487-494. DOI: 10.1034/j.1399-
3054.2001.1120405.x.
[17] N.Smirnoff, The role of active oxygen in the response to water deficit
and desiccation. New Phytol,1993,vol.125,pp. 27-58. DOI:
10.1111/j.1469- 8137.1993.tb03863.x.
[18] H.Soleimanzadeh, D.Habibi, M.R. Ardakani, F.Paknejad, and F.Rejali,
Effect of Potassium Levels on Antioxidant Enzymes and
Malondialdehyde Content under Drought Stress in Sunflower
(Helianthus annuus L.). American Journal of Agricultural and Biological
Sciences,2010,vol. 5 (1), pp. 56-61.
[19] M. Sreekala, T.R. Santosh, and K. Lilitha, Oxidative stress during
selenium deficiency in seedlings of Trigonella foenum-graecum and
mitigation by mimosine. Part I. Hydroperoxide metabolism, Biol. Trace
Element Res,1999,vol.70, pp.193-207.
[20] H.Tapiero ,D.M, Townsend, K.D.Tew, Dossier: Oxidative stress
pathologies and antioxidants: The antioxidant role of selenium and
seleno-compounds. Biomed. Pharmacoth,2003,vol. 57,pp. 134-144.
[21] H.R.Tohidi-Moghadam, A.H. Shirani- Rad, G.Nourmmohamadi,
D.Habibi, M.Mashhadi-Akbar-Boojar, Effect of Super Absorbent
Application on Antioxidant Enzyme Activities in Canola (Brassica
napus L.) Cultivars under Water Stress conditions. American Journal of
Agricultural and Biological Sciences ,2009,vol.4 (3): 215-223.
[22] T.L. Xue, H.Hartikainen, V.Piironen , Antioxidative and growthpromoting
effects of selenium on senescing lettuce. Plant Soil,2001,vol.
237, pp. 55-61.
[23] H.Zahedi, G. Noormohammadi, A. H Shirani-Rad, D. Habibi , M.
Mashhadi Akbar Boojar, Effect of Zeolite and Foliar Application of
Selenium on Growth, Yield and Yield Component of Three Canola
Cultivar under Conditions of Late Season Drought Stress . Not Sci Biol,
2009,vol.1 (1) pp.73-80.
[1] R.L. Foot, Comparative Responses of Field Grown Crops to Phosphate
Concentrations in Soil Solutions. In: Stress Physiology in Crop Plants,
Mussell, H. and R.C. Stapley (Eds.).Wiley,NewYork,2001,pp.81-
106.URL: www.publish.csiro. au/?act=view_file&file_id=AR9760479.
[2] C.H Foyer, M. Lelandais and K.J. Kunert, Photooxidative stress in
plants. Physiol. Plant,1992,vol 92 ,pp.696-717.
http://cat.inist.fr/?aModele=afficheN&cpsidt=3339390.
[3] M.Germ , I. Kreft I, and J. Osvald, Influence of UV-B exclusion and
selenium treatment on photochemical efficiency of photosystem II, yield
and respiratory potential in pumpkins (Cucurbita pepo L.). Plant Physiol.
Biochem,2005,vol. 43,pp. 445-448.
[4] H.Hartikainen , T. Xue, and V.Piironen, Selenium as an antioxidant and
pro-oxidant in ryegrass. Plant Soil,2000, vol .225,pp. 193-200.
[5] A. Hegedus, S. Erdei, and G. Horvath, Comparative studies of H2O2
detoxifying enzymes in green and greening barley seedlings under
cadmium stress. Plant Sci,2001, vol. 160: 1085-1093. DOI:
10.1016/S0168-9452(01)00330-2.
[6] S.M. Johnson, S.J. Doherty, and R.R.D. Croy, Biphasic superoxide
generation in potato Tubers: A self amplifying response to stress. Plant
Physiol,2003, vol 13,pp. 1440-1449.
http://www.plantphysiol.org/cgi/content/abstract/1 31/3/1440.
[7] V.V. Kuznetsov , V.P. Kholodova , V.V. Kuznetsov B.A. Yagodin ,
Selenium regulates the water status of plants exposed to drought. Dokl.
Biol. Sci,2003,vol.390,pp.266-268.
[8] J.Mwanamwenge, S.P. Loss, K.H.M. Siddique, and P.S. Cocks, Effect of
water stress during floral initiation, flowering and podding on the growth
and yield of faba bean (Vicia faba L.). Eur. J. Agron, 1999,vol. 11,pp.1-
11. DOI: 10.1016/S1161- 0301(99)00003-9.
[9] M.Nasri, M.Khalatbari, H.Zahedi, F.Paknejad, and H.R. Tohidi
Moghadam, Evaluation of Micro and Macro Elements in Drought Stress
Condition in Cultivars of Rapeseed (Brassica napus L.). American
Journal of Agricultural and Biological Sciences, 2003,vol.3 (3),pp.579-
583.
[10] G.Noctor, and C.H. Foyer, Ascorbate and glutathione: Keeping active
oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol,1998,
vol.49,pp 249-279. PMID: 15012235.
[11] J,Nowak, K.Kaklewski, M.Ligocki , Influence of selenium on
oxidoreductive enzymes activity in soil and in plants. Soil Biol.
Biochem,2004,vol. 36,pp.1553-1558.
[12] S.Ouchi, A.Nishikawa, and E.Kameda, Soilimproving effect of a superaterabsorbentpolymer
II. Evaporation, leaching of salts and growth of
vegetables. Jap. J. Soil Sci. Plant Nutr,1990,Vol.61,pp.606-613.
http://www.fao.org/agris/search/display.do?f=./199
4/v2007/JP9306919.xml;JP9306919
[13] A.Pennanen, T.Xue, H.Hartikainen , Protective role of selenium in plant
subjected to severe UV irridiation stress. J. Appl. Bot,2002,vol.76,pp.
66-76.
[14] J.G. Scandalios, Oxygen stress and superoxide dismutase. Plant
Physiol,1993,vol.101,pp.712-726.
http://www.pubmedcentral.nih.gov/articlerender.fc gi?artid=158641.
[15] M.Seppanen , M. Turakainen, H.Hartikainen, Selenium effects on
oxidative stress in potato . Plant Science,2003,vol. 165 ,pp.311-/319.
[16] A.Shalata, V. Mittova, M. Volokita, M. Guy, and M. Tal, Response of
the cultivated tomato and its wild salt-tolerant relative Lycopersicon
pennellii to salt-dependent oxidative stress: The root antioxidative
system. Physiol. Plant,2001,vol. 112: 487-494. DOI: 10.1034/j.1399-
3054.2001.1120405.x.
[17] N.Smirnoff, The role of active oxygen in the response to water deficit
and desiccation. New Phytol,1993,vol.125,pp. 27-58. DOI:
10.1111/j.1469- 8137.1993.tb03863.x.
[18] H.Soleimanzadeh, D.Habibi, M.R. Ardakani, F.Paknejad, and F.Rejali,
Effect of Potassium Levels on Antioxidant Enzymes and
Malondialdehyde Content under Drought Stress in Sunflower
(Helianthus annuus L.). American Journal of Agricultural and Biological
Sciences,2010,vol. 5 (1), pp. 56-61.
[19] M. Sreekala, T.R. Santosh, and K. Lilitha, Oxidative stress during
selenium deficiency in seedlings of Trigonella foenum-graecum and
mitigation by mimosine. Part I. Hydroperoxide metabolism, Biol. Trace
Element Res,1999,vol.70, pp.193-207.
[20] H.Tapiero ,D.M, Townsend, K.D.Tew, Dossier: Oxidative stress
pathologies and antioxidants: The antioxidant role of selenium and
seleno-compounds. Biomed. Pharmacoth,2003,vol. 57,pp. 134-144.
[21] H.R.Tohidi-Moghadam, A.H. Shirani- Rad, G.Nourmmohamadi,
D.Habibi, M.Mashhadi-Akbar-Boojar, Effect of Super Absorbent
Application on Antioxidant Enzyme Activities in Canola (Brassica
napus L.) Cultivars under Water Stress conditions. American Journal of
Agricultural and Biological Sciences ,2009,vol.4 (3): 215-223.
[22] T.L. Xue, H.Hartikainen, V.Piironen , Antioxidative and growthpromoting
effects of selenium on senescing lettuce. Plant Soil,2001,vol.
237, pp. 55-61.
[23] H.Zahedi, G. Noormohammadi, A. H Shirani-Rad, D. Habibi , M.
Mashhadi Akbar Boojar, Effect of Zeolite and Foliar Application of
Selenium on Growth, Yield and Yield Component of Three Canola
Cultivar under Conditions of Late Season Drought Stress . Not Sci Biol,
2009,vol.1 (1) pp.73-80.
@article{"International Journal of Biological, Life and Agricultural Sciences:55968", author = "A.R. Pazoki and A. H. Shirani Rad and D. Habibi and F. Paknejad and S. Kobraee and N. Hadayat", title = "Effect of Drought Stress and Selenium Spraying on Superoxide Dismotase Activity of Winter Rapeseed (Brassica napus L.) Cultivars", abstract = "In the other to Study of drought stress and Selenium
spraying effect on superoxide dismotase (SOD) activity of rapeseed
(Brassica napus L.) cultivars in Shahr-e-Rey region, an experiment
carried out in Split factorial design in the basis of randomized
complete blocks with 4 replications in 2006. Irrigation in two levels:
Normal irrigation and irrigation with drought stress when the soil
electrical conductivity reached to 60 as main factor and rapeseed
cultivars in 3 levels Zarfam, Okapi, Opera and selenium spraying at
the beginning of flowering stage in 3 levels: 0, 16 and 21 g/ha as sub
factor.
The results showed that the simple and interaction effect of
irrigation, selenium and cultivars on SOD activity had significant
difference. In this case Zarfam cultivar with 2010 u.mg-1 protein and
Opera with 1454 u.mg-1 protein produced maximum and minimum
amounts of SOD activitiy. Interaction effect of irrigation and variety
showed that, normal irrigation in Opera with 1115 u.mg-1 protein
and drought stress in Zarfam with 2784 u.mg-1 protein conducted to
and minimum and maximum amounts of SOD activity.
Interaction effect of irrigation, cultivar and selenium on SOD
indicated that drought stress condition and 21 gr/ha selenium
spraying in Zarfam variety with 3146 u.mg-1 protein gained to
highest activities of SOD.", keywords = "Drought stress, Rapeseed, Selenium, Superoxide dismutase.", volume = "4", number = "8", pages = "577-4", }
