Abstract: Studies were carried out on the comparative study of the production of Avicelase enzyme using sugarcane bagasse-SCB in two different statuses (i.e. treated and untreated SCB) by thermophilic Geobacillus stearothermophilus at 50ºC. Only four thermophilic bacterial isolates were isolated and assayed for Avicelase production using UntSCB and TSCB. Only one isolate selected as most potent and identified as G. stearothermophilus used in this study. A specific endo-β-1,4-D-glucanase (Avicelase EC 3.2.1.91) was partially purified from a thermophilic bacterial strain was isolated from different soil samples when grown on cellulose enrichment SCB substrate as the sole carbon source. Results shown that G. stearothermophilus was the better Avicelase producer strain. Avicelase had an optimum pH and temperature 7.0 and 50ºC for both UntSCB and TSCB and exhibited good pH stability between "5-8" and "4-9", however, good temperature stability between (30-80ºC) for UntSCB and TSCB, respectively. Other factors affecting the production of Avicelase were compared (i.e. SCB concentration, inoculum size and different incubation periods), all results observed and obtained were revealed that the TSCB was exhibited maximal enzyme activity in comparison with the results obtained from UntSCB, so, the TSCB was enhancing the Avicelase production.
Abstract: It has been proven that early establishment of
microbial flora in digestive tract of ruminants, has a beneficial effect
on their health condition and productivity. A probiotic compound,
made from five bacteria isolated from adult bovine cattle, was dosed
to 15 Holstein newborn calves in order to measure its capacity of
improving body weight gain and reduce diarrhea incidence. The test
was performed in the municipality of Cajicá (Colombia), at 2580
m.a.s.l., throughout rainy season, with environmental temperature
that oscillated between 4 to 25 °C. Five calves were allotted to
control (no addition of probiotic). Treatments 1, and 2 (5 calves per
group) received 10 ml Probiotic mix 1 and 2, respectively. Probiotic
mixes 1 and 2 where similar in microbial composition but different in
production process. Probiotics were added to the morning milk and
dosed on a daily basis by a month and then on a weekly basis for
three additional months. Diarrhea incidence was measured by
observance of number of animals affected in each group; each animal
was weighed up on a daily basis for obtaining weight gain and rumen
fluid samples were extracted with oro-esophageal catheter for
determining level of fiber and grain consumption.
Abstract: Cow milk, is a product of the mammary gland and
soymilk is a beverage made from soybeans; it is the liquid that
remains after soybeans are soaked. In this research effort, we
compared nutritional parameters of this two kind milk such as total
fat, fiber, protein, minerals (Ca, Fe and P), fatty acids, carbohydrate,
lactose, water, total solids, ash, pH, acidity and calories content in
one cup (245 g). Results showed soymilk contains 4.67 grams of fat,
0.52 of fatty acids, 3.18 of fiber, 6.73 of protein, 4.43 of
carbohydrate, 0.00 of lactose, 228.51 of water, 10.40 of total solids
and 0.66 of ash, also 9.80 milligrams of Ca, 1.42 of Fe, and 120.05 of
P, 79 Kcal of calories, pH=6.74 and acidity was 0.24%. Cow milk
contains 8.15 grams of fat, 5.07 of fatty acids, 0.00 of fiber, 8.02 of
protein, 11.37 of carbohydrate, ´Çá4.27 of lactose, 214.69 of water,
12.90 of total solids, 1.75 of ash, 290.36 milligrams of Ca, 0.12 of
Fe, and 226.92 of P, 150 Kcal of calories, pH=6.90 and acidity was
0.21% . Soy milk is one of plant-based complete proteins and cow
milk is a rich source of nutrients as well. Cow milk is containing near
twice as much fat as and ten times more fatty acids do soymilk. Cow
milk contains greater amounts of mineral (except Fe) it contain more
than three hundred times the amount of Ca and nearly twice the
amount of P as does soymilk but soymilk contains more Fe (ten time
more) than does cow milk. Cow milk and soy milk contain nearly
identical amounts of protein and water and fiber is a big plus, dairy
has none. Although what we choose to drink is really a mater of
personal preference and our health objectives but looking at the
comparison, soy looks like healthier choices.
Abstract: In this study, the effect of L-arginine was examined at the neuromuscular junction of the chick biventer cervicis muscle. LArginine at 500 μg/ ml, decreased twitch response to electerical stimulation, and produced rightward shift of the dose- response curve for acetylcholine or carbachol. L-Arginine at 1000μg/ ml produced a strong shift to the right of the dose – response curve for acetylcholine or carbachol with a reduction in the efficacy. The inhibitory effect of L-arginine on the twitch response was blocked by caffeine (200μg/ ml). NO levels were also measured in the chick biventer cervicis muscle homogenates, using spectrophotometric method for the direct detection of NO, nitrite and nitrate. Total nitrite (nitrite + nitrate) was measured by a spectrophotometer at 540 nm after the conversion of nitrate to nitrite by copperized cadmium granules. NO levels were found to be significantly increased in concentrations 500 and 1000μg/ ml of L-arginine in comparison with the control group (p
Abstract: Ten lactating multiparous Holstein cows were used in
a cross-over design with two dietary treatments and 28-d periods
(with 14 d as an adaptation) to study the effect of restaurant fat on
milk production and composition. Each cow was offered 14.7 kg
DM /d of the basal concentrate diet based on barley and corn (crude
protein = 17.7%, neutral detergent fiber = 23.5%, and acid detergent
fiber = 5.8% of dry matter) with free access to alfalfa. Dietary
treatments were arranged as supplying each cow with 0 (CONTROL)
or 150 g/day (RF) of restaurant fat. Supplemental RF did not
significantly (P > 0.25) affect milk yield, composition, and
composition yields, except for milk fat contents. Milk fat contents
were depressed (P < 0.05) with supplemental RF. Our results indicate
that RF could depress milk fat without affecting milk yield and that
the depression in milk fat in response to RF precedes the depression
in milk yield.
Abstract: In order to increase in chickpea quality and
agroecosystem sustainability, field experiments were carried out in
2007 and 2008 growing seasons. In this research the effects of
different organic, chemical and biological fertilizers were
investigated on grain yield and quality of chickpea. Experimental
units were arranged in split-split plots based on randomized complete
blocks with three replications. The highest amounts of yield and yield
components were obtained in G1×N5 interaction. Significant
increasing of N, P, K, Fe and Mg content in leaves and grains
emphasized on superiority of mentioned treatment because each one
of these nutrients has an approved role in chlorophyll synthesis and
photosynthesis ability of the crop. The combined application of
compost, farmyard manure and chemical phosphorus (N5) had the
best grain quality due to high protein, starch and total sugar contents,
low crude fiber and reduced cooking time.
Abstract: To study on effect of PEG and NaCl stress on
germination and early seedling stages on two cultivar of corn, two
separated experiment were laid out at physiology laboratory, faculty
of Agriculture, Razi University, Kermanshah, Iran in 2009. This
investigation was performed as factorial experiment under Complete
Randomized Design (CRD) with three replications. Cultivar factor
contains of two varieties (sweet corn SC403 and Flint corn SC704)
and five levels of stress (0, -2, -4, -6 and -8 bar). The principal aim of
current study was to compare the two varieties of maize in relative to
the stress conditions. Results indicated that significant decrease was
observed in percentage of germination, germination rate, length of
radicle and plumule and radicle and plumule dry matter. On the basis
of the results, NaCl as compared with PEG had more effect on
germination and early seedling stage and sweet corn had more
resistant than flint corn in both stress conditions.
Abstract: In the present study, the response of Nili Ravi buffalo
oocytes to recombinant human follicle stimulating hormone (rhFSH)
(Organon) on meiotic maturation in vitro was examined. Oocytes
were matured in vitro in medium containing either 0 or 0.05 IU/ ml
rhFSH and the stage of nuclear maturation recorded after 24 hours.
The percentage of oocytes in the control group undergoing germinal
vesicle breakdown (GVBD) observed after 24 hours of culture was
29 % whereas as in rhFSH group the percentage was 10 % were at
this stage (P< 0.001).Thus in the presence of rhFSH, a significantly
greater number of oocytes had progressed to the more advanced
stages of nuclear maturation. Indeed, the maturation of GV
(Germinal Vesicle) stage oocytes to the metaphase II (M II) stage
after 24 hours was significantly (P< 0.0001) increased by the
addition of rhFSH (82 % VS 47 %). The percentage of degenerated
oocytes after 24 hours of culture was 24 % in control group, whereas
in rhFSH group the percentage was 8 % after 24 hours. Degeneration
of the oocytes after 24 hours was not significantly (P = 0. 9361)
decreased.
Abstract: Atlantic herring (Clupea harengus) is an important
commercial fish and shows to be more and more demanded for
human consumption. Therefore, it is very important to find good
methods for monitoring the freshness of the fish in order to keep it in
the best quality for human consumption. In this study, the fish was
stored in ice up to 2 weeks. Quality changes during storage were
assessed by the Quality Index Method (QIM), quantitative
descriptive analysis (QDA) and Torry scheme, by texture
measurements: puncture tests and Texture Profile Analysis (TPA)
tests on texture analyzer TA.XT2i, and by electronic nose (e-nose)
measurements using FreshSense instrument. Storage time of herring
in ice could be estimated by QIM with ± 2 days using 5 herring per
lot. No correlation between instrumental texture parameters and
storage time or between sensory and instrumental texture variables
was found. E-nose measurements could be use to detect the onset of
spoilage.
Abstract: The authors report a case of swine urolithiasis caused
by improper administration of sulfamonomethoxine and which was
diagnosed by examination of urinary sediments and analyzing the
composition of the uroliths. The chemical composition of urinary
calculi obtained from affected pigs with urolithiasis was further
confimed as sulfamonomethoxine by fourier transform infrared
(FTIR). It is suggested that appearance of typical fanlike or wheat
bunchy crystals in urinary sediments under observation of lightmicroscope
and determination by FTIR for the crystals are helpful in
diagnosing sulfa calculi causced swine urolithiasis.
Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: We have developed an energy based approach for identifying the binding sites and important residues for binding in protein-protein complexes. We found that the residues and residuepairs with charged and aromatic side chains are important for binding. These residues influence to form cation-¤Ç, electrostatic and aromatic interactions. Our observation has been verified with the experimental binding specificity of protein-protein complexes and found good agreement with experiments. The analysis on surrounding hydrophobicity reveals that the binding residues are less hydrophobic than non-binding sites, which suggests that the hydrophobic core are important for folding and stability whereas the surface seeking residues play a critical role in binding. Further, the propensity of residues in the binding sites of receptors and ligands, number of medium and long-range contacts, and influence of neighboring residues will be discussed.
Abstract: Influence of octane and benzene on plant cell
ultrastructure and enzymes of basic metabolism, such as nitrogen
assimilation and energy generation have been studied. Different
plants: perennial ryegrass (Lolium perenne) and alfalfa (Medicago
sativa); crops- maize (Zea mays L.) and bean (Phaseolus vulgaris);
shrubs – privet (Ligustrum sempervirens) and trifoliate orange
(Poncirus trifoliate); trees - poplar (Populus deltoides) and white
mulberry (Morus alba L.) were exposed to hydrocarbons of different
concentrations (1, 10 and 100 mM). Destructive changes in bean and
maize leaves cells ultrastructure under the influence of benzene
vapour were revealed at the level of photosynthetic and energy
generation subcellular organells. Different deviations at the level of
subcellular organelles structure and distribution were observed in
alfalfa and ryegrass root cells under the influence of benzene and
octane, absorbed through roots. The level of destructive changes is
concentration dependent. Benzene at low 1 and 10 mM concentration
caused the increase in glutamate dehydrogenase (GDH) activity in
maize roots and leaves and in poplar and mulberry shoots, though to
higher extent in case of lower, 1mM concentration. The induction
was more intensive in plant roots. The highest tested 100mM
concentration of benzene was inhibitory to the enzyme in all plants.
Octane caused induction of GDH in all grassy plants at all tested
concentrations; however the rate of induction decreased parallel to
increase of the hydrocarbon concentration. Octane at concentration 1
mM caused induction of GDH in privet, trifoliate and white mulberry
shoots. The highest, 100mM octane was characterized by inhibitory
effect to GDH activity in all plants. Octane had inductive effect on
malate dehydrogenase in almost all plants and tested concentrations,
indicating the intensification of Trycarboxylic Acid Cycle.
The data could be suggested for elaboration of criteria for plant
selection for phytoremediation of oil hydrocarbons contaminated
soils.
Abstract: The experiment was conducted to evaluate
digestibility quantities of protein in Canola Meals (CMs) between
caecectomised and intact adult Rhode Island Red (RIR) cockerels
with using conventional addition method (CAM) for 7 d: a 4-d
adaptation and a 3-d experiment period on the basis of a completely
randomized design with 4 replicates. Results indicated that
caecectomy decreased (P
Abstract: Ligula intestinalis is a three-host life-cycle
Pseudophyllidean Cestode which in its plerocercoid stage infests a
range of fresh water species. The objective of the present study was
the worm occurrence within planctonic copepods, fishes and
piscivorous birds and examine of parasite-hosts samples in the Lake
of Sattarkhan Dam (near the city of Ahar, East Azerbaijan, Iran).
Fish sample were collected with fyke and gill nets and the abdominal
cavity was examined for the presence of ligula. Zooplanktons were
captured using a planktonic net and occurrence of parasitic larval
form in the body cavity was determined. Piscivorous birds were
selected by telescope, they hunted and dissected for presence of
parasite eggs in their gut. Results indicated that prevalence of
infection was 16% for cyclopid copepoda and majority of infected
cyclopid were female Cyclops. Investigation of 310 fishes specimens
were indicated to infection of five species of cyprinid fishes. In
addition, results indicated to manipulation of six species of migratory
aquatic and semi aquatic birds by ligula. Obtained results are in
agreement by previous studies. Its definite in this study that all of
fishes in Sattarkhan Lake capable to infection, its important for
health because they capture by native people and it is documented
that ligula can be introduce as a zoonose. It's seemed that to prevent
from disperses of parasite and restricted of infection, biological
elimination can be effective and it's necessary to inform native
people about sanitation.
Abstract: The case study was conducted to show the effect of milking method in goat called half day milking on the milk production and the growth of kids. Data were collected by interviewing farmers and investigating goat production in the communal goat housing from June 2008 to May 2009. The interview was conducted to collect data about goat management. The observations were conducted on 10 goats, which were selected based on the uniformity of age, number of kid born/goat and the milking method in practice. The samples were divided into two groups; those were full 3 months nursing and half day milked goats (in this group the kids were separated from goat during the previous night milking and then the kids were allowed to suck the goat during the day). The result showed that the communal goat housing had 138 goats and 25% of the farmers milked the goat. The implementation of half day milking increased the milk production significantly (P
Abstract: Using 1km grid datasets representing monthly mean
precipitation, monthly mean temperature, and dry matter production
(DMP), we considered the regional plant production ability in
Southeast and South Asia, and also employed pixel-by-pixel
correlation analysis to assess the intensity of relation between climate
factors and plant production. While annual DMP in South Asia was
approximately less than 2,000kg, the one in most part of Southeast
Asia exceeded 2,500 - 3,000kg. It suggested that plant production in
Southeast Asia was superior to South Asia, however, Rain-Use
Efficiency (RUE) representing dry matter production per 1mm
precipitation showed that inland of Indochina Peninsula and India
were higher than islands in Southeast Asia. By the results of
correlation analysis between climate factors and DMP, while the area
in most parts of Indochina Peninsula indicated negative correlation
coefficients between DMP and precipitation or temperature, the area
in Malay Peninsula and islands showed negative correlation to
precipitation and positive one to temperature, and most part of India
dominating South Asia showed positive to precipitation and negative
to temperature. In addition, the areas where the correlation coefficients
exceeded |0.8| were regarded as “susceptible" to climate factors, and
the areas smaller than |0.2| were “insusceptible". By following the
discrimination, the map implying expected impacts by climate change
was provided.
Abstract: The great challenge of the agricultural sector is to
produce more crop from less water, which can be achieved by
increasing crop water productivity. The modernization of the
irrigation systems offers a number of possibilities to expand the
economic productivity of water and improve the virtual water status.
The objective of the present study is to assess the global water
productivity (GWP) within the major irrigation command areas of
I.R. Iran. For this purpose, fourteen irrigation command areas where
located in different areas of Iran were selected. In order to calculate
the global water productivity of irrigation command areas, all data on
the delivered water to cropping pattern, cultivated area, crops water
requirement, and yield production rate during 2002-2006 were
gathered. In each of the command areas it seems that the cultivated
crops have a higher amount of virtual water and thus can be replaced
by crops with less virtual water. This is merely suggested due to crop
water consumption and at the time of replacing crops, economic
value as well as cultural and political factors must be considered. The
results indicated that the lowest GWP belongs to Mahyar and
Borkhar irrigation areas, 0.24 kg m-3, and the highest is that of the
Dez irrigation area, 0.81 kg m-3. The findings demonstrated that
water management in the two irrigation areas is just efficient. The
difference in the GWP of irrigation areas is due to variations in the
cropping pattern, amount of crop productions, in addition to the
effective factors in the water use efficiency in the irrigation areas.