Abstract: In this study, we are interested in a species of the
family of Asteraceae (Tagetes erecta). This family is considered as a
source of antimicrobial extracts with strong capacity. The extraction
of the flavonoids is carried out by the method of liquid/liquid with the
use of successive solvents. Afterwards, we evaluated the biological
activity of the flavonoids on five pathogenic bacterial stocks such as
Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae,
Pseudomonas aeruginosa and Staphylococcus aureus and two stocks
of yeasts to knowing Candida albicans) and Saccharomyces
cerevisiae, by employing the method of the aromatogramme starting
from a solid disc. The result of the antimicrobial activity shows an
action and a variable degree of sensitivity according to bacterial
stocks tested. It will be noted that the flavonoids have an inhibiting
effect on E. coli, B. subtilis, K. pneumoniae and S. aureus. But a
resistance with respect to the extract by P. aeruginosa, C. albicans
and S. cerevisiae is to be mentioned.
Abstract: A feeding experiment was conducted to determine the
optimum dietary protein and lipid levels for juvenile fancy carp. Eight
experimental diets were formulated to contain four protein levels (200,
300, 400 and 500 g kg-1) with two lipid levels (70 and 140 g kg-1).
Triplicate groups of fish (initial weight, 12.1±0.2 g fish-1) were
hand-fed the diets to apparent satiation for 8 weeks. Fish growth
performance, feed utilization and feed intake were significantly
(P0.05). Weight gain and feed efficiency ratio tended to
increase as dietary protein level increased up to 400 and 500 g kg-1,
respectively. Daily feed intake of fish decreased with increasing
dietary protein level and that of fish fed diet contained 500 g kg-1
protein was significantly lower than other fish groups. The protein
efficiency ratio of fish fed 400 and 500 g kg-1 protein was lower than
that of fish fed 200 and 300 g kg-1 protein. Moisture, crude protein and
crude lipid contents of muscle and liver were significantly affected by
dietary protein, but not by dietary lipid level (P>0.05). The increase in
dietary lipid level resulted in an increase in linoleic acid in liver and
muscle paralleled with a decrease in n-3 highly unsaturated fatty acids
content in muscle of fish. In considering these results, it was concluded
that the diet containing 400 g kg-1 protein with 70 g kg-1 lipid level is
optimal for growth and efficient feed utilization of juvenile fancy carp.
Abstract: Monoamine oxidase A gene (MAOA) is suggested to
be a candidate gene implicated in many neuropsychiatric disorders,
including autism spectrum disorder (ASD). This meta-analytic review
evaluates the relationship between ASD and MAOA markers such as
30 bp variable number tandem repeats in the promoter region
(uVNTR) and single nucleotide polymorphisms (SNPs) by using
findings from recently published studies. It seems that in Caucasian
males, the risk of developing ASD increase with the presence of 4-
repeat allele in the promoter region of MAOA gene whereas no
differences were found between autistic patients and controls in
Egyptian, West Bengal and Korean population. Some studies point to
the importance of specific haplotype groups of SNPs and interaction
of MAOA with others genes (e. g. FOXP2 or SRY). The results of
existing studies are insufficient and further research is needed.
Abstract: The Roma (Gypsies) is a transnational minority with a
high degree of consanguineous marriages. Similar to other
genetically isolated founder populations, the Roma harbor a number
of unique or rare genetic disorders. This paper discusses about a rare
form of Charcot-Marie-Tooth disease – type 4G (CMT4G), also
called Hereditary Motor and Sensory Neuropathy type Russe, an
autosomal recessive disease caused by mutation private to Roma
characterized by abnormally increased density of non-myelinated
axons. CMT4G was originally found in Bulgarian Roma and in 2009
two putative causative mutations in the HK1 gene were identified.
Since then, several cases were reported in Roma families mainly
from Bulgaria and Spain. Here we present a Slovak Roma family in
which CMT4G was diagnosed on the basis of clinical examination
and genetic testing. This case is a further proof of the role of the HK1
gene in pathogenesis of the disease. It confirms that mutation in the
HK1 gene is a common cause of autosomal recessive CMT disease in
Roma and should be considered as a common part of a diagnostic
procedure.
Abstract: A strain of Monascus purpureus CMU001 was used to
prepare red yeast rice from Thai glutinous rice Korkor 6 (RD 6).
Adding of different amounts of histidine (156, 312, 625 and 1250 mg
in 100 g of rice grains)) under aerobic and air limitation (air-lock)
condition were used in solid fermentation. Determination of the yield
as well as monacolin K content was done. Citrinin content was also
determined in order to confirm the safety use of prepared red yeast
rice. It was found that under air-lock condition with 1250 mg of
histidine addition gave the highest yield of 37.40 g of dried red yeast
rice prepared from 100 g of rice. Highest 5.72 mg content of
monacolin K was obtained under air-lock condition with 312 mg
histidine addition. In the other hand, citrinin content was found to be
less than 24462 ng/g of all dried red yeast rice samples under the
experimental methods used in this work.
Abstract: This paper investigates the activity of the rectus
femoris (RF) and biceps femoris (BF) in healthy subjects during salat
(prostration) and specific exercise (squat exercise) using
electromyography (EMG). A group of undergraduates aged between
19 to 25 years voluntarily participated in this study. The myoelectric
activity of the muscles were recorded and analyzed. The finding
indicated that there were contractions of the muscles during the salat
and exercise with almost same EMG’s level. From the result,
Wilcoxon’s Rank Sum test showed significant difference between
prostration and squat exercise (p
Abstract: Risperidone (RISP) is an antipsychotic agent and has
low water solubility and nontargeted delivery results in numerous
side effects. Hence, an attempt was made to develop SLNs hydrogel
for intranasal delivery of RISP to achieve maximum bioavailability
and reduction of side effects. RISP loaded SLNs composed of 1.65%
(w/v) lipid mass were produced by high shear homogenization (HSH)
coupled ultrasound (US) method using glycerylmonostearate (GMS)
or Imwitor 900K (solid lipid). The particles were loaded with 0.2%
(w/v) of the RISP & surface-tailored with a 2.02% (w/v) non-ionic
surfactant Tween® 80. Optimization was done using 32 factorial
design using Design Expert® software. The prepared SLNs
dispersion incorporated into Polycarbophil AA1 hydrogel (0.5%
w/v). The final gel formulation was evaluated for entrapment
efficiency, particle size, rheological properties, X ray diffraction, in
vitro diffusion, ex vivo permeation using sheep nasal mucosa and
histopathological studies for nasocilliary toxicity. The entrapment
efficiency of optimized SLNs was found to be 76 ± 2%,
polydispersity index
Abstract: The aim of this study was to design and simulate a
particular type of Asynchronous State Machine (ASM), namely a
‘traffic light controller’ (TLC), operated at a frequency of 0.5 Hz.
The design task involved two main stages: firstly, designing a 4-bit
binary counter using J-K flip flops as the timing signal and,
subsequently, attaining the digital logic by deploying ASM design
process. The TLC was designed such that it showed a sequence of
three different colours, i.e. red, yellow and green, corresponding to
set thresholds by deploying the least number of AND, OR and NOT
gates possible. The software Multisim was deployed to design such
circuit and simulate it for circuit troubleshooting in order for it to
display the output sequence of the three different colours on the
traffic light in the correct order. A clock signal, an asynchronous 4-
bit binary counter that was designed through the use of J-K flip flops
along with an ASM were used to complete this sequence, which was
programmed to be repeated indefinitely. Eventually, the circuit was
debugged and optimized, thus displaying the correct waveforms of
the three outputs through the logic analyser. However, hazards
occurred when the frequency was increased to 10 MHz. This was
attributed to delays in the feedback being too high.
Abstract: In this paper, Bayesian online inference in models of
data series are constructed by change-points algorithm, which
separated the observed time series into independent series and study
the change and variation of the regime of the data with related
statistical characteristics. variation of statistical characteristics of time
series data often represent separated phenomena in the some
dynamical system, like a change in state of brain dynamical reflected
in EEG signal data measurement or a change in important regime of
data in many dynamical system. In this paper, prediction algorithm
for studying change point location in some time series data is
simulated. It is verified that pattern of proposed distribution of data
has important factor on simpler and smother fluctuation of hazard
rate parameter and also for better identification of change point
locations. Finally, the conditions of how the time series distribution
effect on factors in this approach are explained and validated with
different time series databases for some dynamical system.
Abstract: Bacterial strains capable of degradation of malathion
from the domestic sewage were isolated by an enrichment culture
technique. Three bacterial strains were screened and identified as
Acinetobacter baumannii (AFA), Pseudomonas aeruginosa (PS1),
and Pseudomonas mendocina (PS2) based on morphological,
biochemical identification and 16S rRNA sequence analysis.
Acinetobacter baumannii AFA was the most efficient malathion
degrading bacterium, so used for further biodegradation study. AFA
was able to grow in mineral salt medium (MSM) supplemented with
malathion (100 mg/l) as a sole carbon source, and within 14 days,
84% of the initial dose was degraded by the isolate measured by high
performance liquid chromatography. Strain AFA could also degrade
other organophosphorus compounds including diazinon, chlorpyrifos
and fenitrothion. The effect of different culture conditions on the
degradation of malathion like inoculum density, other carbon or
nitrogen sources, temperature and shaking were examined.
Degradation of malathion and bacterial cell growth were accelerated
when culture media were supplemented with yeast extract, glucose
and citrate. The optimum conditions for malathion degradation by
strain AFA were; an inoculum density of 1.5x 10^12CFU/ml at 30°C
with shaking. A specific polymerase chain reaction primers were
designed manually using multiple sequence alignment of the
corresponding carboxylesterase enzymes of Acinetobacter species.
Sequencing result of amplified PCR product and phylogenetic
analysis showed low degree of homology with the other
carboxylesterase enzymes of Acinetobacter strains, so we suggested
that this enzyme is a novel esterase enzyme. Isolated bacterial strains
may have potential role for use in bioremediation of malathion
contaminated.
Abstract: Analyzing DNA microarray data sets is a great
challenge, which faces the bioinformaticians due to the complication
of using statistical and machine learning techniques. The challenge
will be doubled if the microarray data sets contain missing data,
which happens regularly because these techniques cannot deal with
missing data. One of the most important data analysis process on
the microarray data set is feature selection. This process finds the
most important genes that affect certain disease. In this paper, we
introduce a technique for imputing the missing data in microarray
data sets while performing feature selection.
Abstract: An experiment was conducted to determine the effect
of pollination on seed quality of rapeseed in Chitwan, Nepal during
2012-2013. The experiment was designed in Randomized Complete
Block with four replications and five treatments. The rapeseed plots
were caged with mosquito nets at 10% flowering except natural
pollination. Two-framed colonies of Apis mellifera L. and Apis
cerana F. were introduced separately for pollination, and control plot
caged without pollinators. The highest germination percent was
observed on Apis cerana F. pollinated plot seeds (90.50%
germination) followed by Apis mellifera L. pollinated plots (87.25 %)
and lowest on control plots (42.00% germination) seeds. Similarly,
seed test weight of Apis cerana F. pollinated plots (3.22 gm/ 1000
seed) and Apis mellifera L. pollinated plots (2.93 gm/1000 seed) were
and lowest on control plots (2.26 gm/ 1000 seed) recorded. Likewise,
oil content was recorded highest on pollinated by Apis cerana F.
(36.1%) followed by pollinated by Apis mellifera L. (35.4%) and
lowest on control plots (32.8%). This study clearly indicated
pollination increases the seed quality of rapeseed and therefore,
management of honeybee is necessary for producing higher quality of
rapeseed under Chitwan condition.
Abstract: Human leukocyte antigen (HLA) typing from next
generation sequencing (NGS) data has the potential for applications in
clinical laboratories and population genetic studies. Here we introduce
a novel technique for HLA typing from NGS data based on
read-mapping using a comprehensive reference panel containing all
known HLA alleles and de novo assembly of the gene-specific short
reads. An accurate HLA typing at high-digit resolution was achieved
when it was tested on publicly available NGS data, outperforming
other newly-developed tools such as HLAminer and PHLAT.
Abstract: Development of a method to estimate gene functions is
an important task in bioinformatics. One of the approaches for the
annotation is the identification of the metabolic pathway that genes are
involved in. Since gene expression data reflect various intracellular
phenomena, those data are considered to be related with genes’
functions. However, it has been difficult to estimate the gene function
with high accuracy. It is considered that the low accuracy of the
estimation is caused by the difficulty of accurately measuring a gene
expression. Even though they are measured under the same condition,
the gene expressions will vary usually. In this study, we proposed a
feature extraction method focusing on the variability of gene
expressions to estimate the genes' metabolic pathway accurately. First,
we estimated the distribution of each gene expression from replicate
data. Next, we calculated the similarity between all gene pairs by KL
divergence, which is a method for calculating the similarity between
distributions. Finally, we utilized the similarity vectors as feature
vectors and trained the multiclass SVM for identifying the genes'
metabolic pathway. To evaluate our developed method, we applied the
method to budding yeast and trained the multiclass SVM for
identifying the seven metabolic pathways. As a result, the accuracy
that calculated by our developed method was higher than the one that
calculated from the raw gene expression data. Thus, our developed
method combined with KL divergence is useful for identifying the
genes' metabolic pathway.
Abstract: With drug resistance becoming widespread in
Plasmodium falciparum infections, the development of the alternative
drugs is the desired strategy for prevention and cure of malaria. Three
drug targets were selected to screen promising drug molecules from
the GSK library of 13469 molecules. Using an in silico structure-based
drug designing approach, the differences in binding energies of
the substrate and inhibitor were exploited between target sites of
parasite and human to design a drug molecule against Plasmodium.
The docking studies have shown several promising molecules from
GSK library with more effective binding as compared to the already
known inhibitors for the drug targets. Though stronger interaction has
been shown by several molecules as compared to the reference, few
molecules have shown the potential as drug candidates though in
vitro studies are required to validate the results. In case of
thymidylate synthase-dihydrofolatereductase (TS-DHFR), three
compounds have shown promise for future studies as potential drugs.
Abstract: Lipases constitute one of the most important groups of
industrial enzymes that catalyze the hydrolysis of triacylglycerol to
glycerol and fatty acids. Muscarinic antagonist relieves smooth
muscle spasm of the gastrointestinal tract and effect on the
cardiovascular system. In this research the effect of a muscarinic
antagonist on the lipase activity of Pseudomonas aeruginosa was
studied. Lineweaver–Burk plot showed that the drug inhibited the
enzyme by competitive inhibition. The IC50 value (0.16 mM) and Ki
(0.03 mM) of the drug revealed the drug bound to enzyme with high
affinity. Determination of enzyme activity in various pH and
temperature showed that the maximum activity of lipase was at pH 8
and 60oC both in presence and absence of the drug.
Abstract: Skin aging is a slow multifactorial process influenced
by both internal as well as external factors. Ultra-violet radiations
(UV), diet, smoking and personal habits are the most common
environmental factors that affect skin aging. Fat contents and fibrous
proteins as collagen and elastin are core internal structural
components. The direct influence of UV on elastin integrity and
health is central on aging of skin especially by time. The deposition
of abnormal elastic material is a major marker in a photo-aged skin.
Searching for compounds that may protect against cutaneous photodamage
is exceedingly valued. Retinoids and alpha hydroxy acids
have been endorsed by some researchers as possible candidates for
protecting and or repairing the effect of UV damaged skin. For
consolidating a better system of anti- and protective effects of such
anti-aging agents, we evaluated the combinatory effects of various
dosages of lactic acid and retinol on the dermal fibroblast’s elastin
levels exposed to UV. The UV exposed cells showed significant
reduction in the elastin levels. A combination of drugs with a higher
concentration of lactic acid (30 -35 mM) and a lower concentration of
retinol (10-15mg/mL) showed to work better in maintaining elastin
concentration in UV exposed cells. We assume this preservation
could be the result of increased tropo-elastin gene expression
stimulated by retinol whereas lactic acid probably repaired the UV
irradiated damage by enhancing the amount and integrity of the
elastin fibers.
Abstract: Pulmonary Function Tests are important non-invasive
diagnostic tests to assess respiratory impairments and provides
quantifiable measures of lung function. Spirometry is the most
frequently used measure of lung function and plays an essential role
in the diagnosis and management of pulmonary diseases. However,
the test requires considerable patient effort and cooperation,
markedly related to the age of patients resulting in incomplete data
sets. This paper presents, a nonlinear model built using Multivariate
adaptive regression splines and Random forest regression model to
predict the missing spirometric features. Random forest based feature
selection is used to enhance both the generalization capability and the
model interpretability. In the present study, flow-volume data are
recorded for N= 198 subjects. The ranked order of feature importance
index calculated by the random forests model shows that the
spirometric features FVC, FEF25, PEF, FEF25-75, FEF50 and the
demographic parameter height are the important descriptors. A
comparison of performance assessment of both models prove that, the
prediction ability of MARS with the `top two ranked features namely
the FVC and FEF25 is higher, yielding a model fit of R2= 0.96 and
R2= 0.99 for normal and abnormal subjects. The Root Mean Square
Error analysis of the RF model and the MARS model also shows that
the latter is capable of predicting the missing values of FEV1 with a
notably lower error value of 0.0191 (normal subjects) and 0.0106
(abnormal subjects) with the aforementioned input features. It is
concluded that combining feature selection with a prediction model
provides a minimum subset of predominant features to train the
model, as well as yielding better prediction performance. This
analysis can assist clinicians with a intelligence support system in the
medical diagnosis and improvement of clinical care.
Abstract: Morphological interaction of porcine cumulus-oocyte
complexes (pCOCs) was investigated on in vitro condition using
electron microscope (SEM and TEM). The totals of 1,923 oocytes
were round in shape, surrounded by Zona pellucida with layer of
cumulus cells ranging between 59.29-202.14 μm in size. They were
classified into intact-, multi-, partial cumulus cell layer oocyte, and
completely denuded oocyte, at the percentage composition of 22.80%
32.70%, 18.60%, and 25.90 % respectively. The pCOCs classified as
intact- and multi cumulus cell layer oocytes were further culturing at
37°C with 5% CO2, 95% air atmosphere and high humidity for 44 h
in M199 with Earle’s salts supplemented with 10% HTFCS, 2.2
mg/mL NaHCO3, 1 M Hepes, 0.25 mM pyruvate, 15 μg/mL porcine
follicle-stimulating hormone, 1 μg/mL LH, 1μg/mL estradiol with
ethanol, and 50 μg/mL gentamycin sulfate. On electron microscope
study, cumulus cells were found to stick their processes to secrete
substance from the sac-shape end into Zona pellucida of the oocyte
and also communicated with the neighboring cells through their
microvilli on the beginning of incubation period. It is believed that
the cumulus cells communicate with the oocyte by inserting the
microvilli through this gap and embedded in the oocyte cytoplasm
before secreting substance, through the sac-shape end of the
microvilli, to inhibit primary oocyte development at the prophase I.
Morphological changes of the complexes were observed after
culturing for 24-44 h. One hundred percentages of the cumulus layers
were expanded and cumulus cells were peeling off from the oocyte
surface. In addition, the round-shape cumulus cells transformed
themselves into either an elongate shape or a columnar shape, and no
communication between cumulus neighboring cells. After 44 h of
incubation time, diameter of oocytes surrounded by cumulus cells
was larger than 0 h incubation. The effect of hormones in culture
medium is exerted by their receptors present in porcine oocyte. It is
likely that all morphological changes of the complexes after hormone
treatment were to allow maturation of the oocyte. This study
demonstrated that the association of hormones in M199 could
promote porcine follicle activation in 44 h in vitro condition. This
culture system should be useful for studying the regulation of early
follicular growth and development, especially because these follicles represent a large source of oocytes that could be used in vitro for cell
technology.
Abstract: This paper presents system level CMOS solid-state
nanopore techniques enhancement for speedup next generation
molecular recording and high throughput channels. This discussion
also considers optimum number of base-pair (bp) measurements
through channel as an important role to enhance potential read
accuracy. Effective power consumption estimation offered suitable
range of multi-channel configuration. Nanopore bp extraction model
in statistical method could contribute higher read accuracy with
longer read-length (200 < read-length). Nanopore ionic current
switching with Time Multiplexing (TM) based multichannel readout
system contributed hardware savings.