Abstract: T7 phage could be used as a perfect vector for peptides expression and haptens presentation. T7-3GnRH recombinant phage was constructed by inserting three copies of Gonadotrophin Releasing Hormone (GnRH) gene into the multiple cloning site of T7 Select 415-1b phage genome. The positive T7-3GnRH phage was selected by using polymerase chain reaction amplification, and the p10B-3GnRH fusion protein was verified by SDS-PAGE and Western-blotting assay. T7-3GnRH vaccine was made and immunized with 1010 pfu in 0.2 ml per dose in mice. Blood samples were collected at an interval in weeks, and anti-GnRH antibody and testosterone concentrations were detected by ELISA and radioimmunoassay, respectively. The results show that T7-3GnRH phage particles confer a high immunogenicity to the GnRH-derived epitope. Moreover, the T7-3GnRH vaccine induced higher level of anti-GnRH antibody than ImproVac®. However, the testosterone concentrations in both immunized groups were at a similar level, and the testis developments were significantly inhibited compared to controls. These findings demonstrated that the anti-GnRH antibody could neutralize the endogenous GnRH to down regulate testosterone level and limit testis development, highlighting the potential value of T7-3GnRH in the immunocastration vaccine research.
Abstract: Human leukocyte antigen (HLA) typing from next
generation sequencing (NGS) data has the potential for applications in
clinical laboratories and population genetic studies. Here we introduce
a novel technique for HLA typing from NGS data based on
read-mapping using a comprehensive reference panel containing all
known HLA alleles and de novo assembly of the gene-specific short
reads. An accurate HLA typing at high-digit resolution was achieved
when it was tested on publicly available NGS data, outperforming
other newly-developed tools such as HLAminer and PHLAT.
Abstract: This research attempts to study the feasibility of
augmenting an augmented reality (AR) image card on a Quick
Response (QR) code. The authors have developed a new visual tag,
which contains a QR code and an augmented AR image card. The new
visual tag has features of reading both of the revealed data of the QR
code and the instant data from the AR image card. Furthermore, a
handheld communicating device is used to read and decode the new
visual tag, and then the concealed data of the new visual tag can be
revealed and read through its visual display. In general, the QR code is
designed to store the corresponding data or, as a key, to access the
corresponding data from the server through internet. Those reveled
data from the QR code are represented in text. Normally, the AR
image card is designed to store the corresponding data in
3-Dimensional or animation/video forms. By using QR code's
property of high fault tolerant rate, the new visual tag can access those
two different types of data by using a handheld communicating device.
The new visual tag has an advantage of carrying much more data than
independent QR code or AR image card. The major findings of this
research are: 1) the most efficient area for the designed augmented AR
card augmenting on the QR code is 9% coverage area out of the total
new visual tag-s area, and 2) the best location for the augmented AR
image card augmenting on the QR code is located in the bottom-right
corner of the new visual tag.