The Construction of a Probiotic Lactic Acid Bacterium Expressing Acid-Resistant Phytase Enzyme

Year: 2015 Volume: 9 Issue: 8 963 - 968 Pages

• Authors:
• R. Majidzadeh Heravi
• M. Sankian
• H. Kermanshahi
• M. R. Nassiri
• A. Heravi Moussavi
• S. A. Lari
• A. R. Varasteh

Abstract: The use of probiotics engineered to express specific enzymes has been the subject of considerable attention in poultry industry because of increased nutrient availability and reduced cost of enzyme supplementation. Phytase enzyme is commonly added to poultry feed to improve digestibility and availability of phosphorus from plant sources. To construct a probiotic with potential of phytate degradation, phytase gene (appA) from E. coli was cloned and transformed into two probiotic bacteria Lactobacillus salivarius and Lactococcus lactis. L. salivarous showed plasmid instability, unable to express the gene. The expression of appA gene in L. lactis was analyzed by detecting specific RNA and zymography assay. Phytase enzyme was isolated from cellular extracts of recombinant L. lactis, showing a 46 kDa band upon the SDS-PAGE analysis. Zymogram also confirmed the phytase activity of the 46 kDa band corresponding to the enzyme. An enzyme activity of 4.9U/ml was obtained in cell extracts of L. lactis. The growth of native and recombinant L. lactis was similar in the presence of two concentrations of ox bile.

• Keywords:
• Lactobacillus salivarus
• Lactococcus lactis
• recombinant
• phytase
• poultry.

Water Quality Assessment Based on Operational Indicator in West Coastal Water of Malaysia

Year: 2016 Volume: 10 Issue: 8 877 - 882 Pages

• Authors:
• Seyedeh Belin Tavakoly Sany
• H. Rosli
• R. Majid
• S. Aishah

Abstract: In this study, water monitoring was performed from Nov. 2012 to Oct. 2013 to assess water quality and evaluate the spatial and temporal distribution of physicochemical and biological variables in water. Water samples were collected from 10 coastal water stations of West Port. In the case of water-quality assessment, multi-metric indices and operational indicators have been proposed to classify the trophic status at different stations. The trophic level of West Port coastal water ranges from eutrophic to hypertrophic. Chl-a concentration was used to estimate the biological response of phytoplankton biomass and indicated eutrophic conditions in West Port and mesotrophic conditions at the control site. During the study period, no eutrophication events or secondary symptoms occurred, which may be related to hydrodynamic turbulence and water exchange, which prevent the development of eutrophic conditions in the West Port.

• Keywords:
• Water quality
• multi-metric indices
• operational indicator
• Malaysia
• West Port.