Abstract: Microbes have been used to solve environmental
problems for many years. The role of microorganism to sequester,
precipitate or alter the oxidation state of various heavy metals has
been extensively studied. Treatment using microorganism interacts
with toxic metal are very diverse. The purpose of this research is to
remove the mercury using Pseudomonas putida (P. putida), pure
culture ATTC 49128 at optimum growth parameters such as
techniques of culture, acclimatization time and speed of incubator
shaker. Thus, in this study, the optimum growth parameters of P.
putida were obtained to achieve the maximum of mercury removal.
Based on the optimum parameters of P. putida for specific growth
rate, the removal of two different mercury concentration, 1 ppm and
4 ppm were studied. From mercury nitrate solution, a mercuryresistant
bacterial strain which is able to reduce from ionic mercury
to metallic mercury was used to reduce ionic mercury. The overall
levels of mercury removal in this study were between 80% and 89%.
The information obtained in this study is of fundamental for
understanding of the survival of P. putida ATTC 49128 in mercury
solution. Thus, microbial mercury removal is a potential
bioremediation for wastewater especially in petrochemical industries
in Malaysia.
Abstract: This study was carried out to investigate the adverse
effect of industrial wastewater on surface water quality in Gebeng
industrial estate, Pahang, Malaysia. Surface water was collected from
six sampling stations. Physicochemical parameters were
characterized based on in-situ and ex-situ analysis according to
standard methods by American Public Health Association (APHA).
Selected heavy metals were determined by using Inductively Coupled
Plasma Mass Spectrometry (ICP MS). The results revealed that the
concentration of heavy metals such as Pb, Cu, Cd, Cr and Hg were
high in samples. The results also showed that the value of Pb and Hg
were higher in the wet season in comparison to dry season.
According to Malaysia National Water Quality Standard (NWQS)
and Water Quality Index (WQI) all the sampling station were
categorized as class IV (highly polluted). The present study revealed
that the adverse effects of careless disposal of wastes and directly
discharge of effluents affected on surface water quality. Therefore,
the authorities should implement the laws to ensure the proper
practices of wastewater management for environmental sustainability
around the study area.