Abstract: This paper studied the synthesis of monoacylglycerol (monolaurin) by glycerolysis of coconut oil and crude glycerol, catalyzed by Carica papaya lipase. Coconut oil obtained from cold pressed extraction method and crude glycerol obtained from the biodiesel plant in Department of Chemistry, Uttaradit Rajabhat University, Thailand which used oils were used as raw materials for biodiesel production through transesterification process catalyzed by sodium hydroxide. The influences of the following variables were studied: (i) type of organic solvent, (ii) molar ratio of substrate, (iii) reaction temperature, (iv) reaction time, (v) lipase dosage, and (vi) initial water activity of enzyme. High yields in monoacylglycerol (58.35%) were obtained with molar ratio of glycerol to oil at 8:1 in ethanol, temperature was controlled at 45oC for 36 hours, the amount of enzyme used was 20 wt% of oil and initial water activity of enzyme at 0.53.
Abstract: In this paper, naturally immobilized lipase, Carica
papaya lipase, catalyzed biodiesel production from fish oil was
studied. The refined fish oil, extracted from the discarded parts of
fish, was used as a starting material for biodiesel production. The
effects of molar ratio of oil: methanol, lipase dosage, initial water
activity of lipase, temperature and solvent were investigated. It was
found that Carica papaya lipase was suitable for methanolysis of fish
oil to produce methyl ester. The maximum yield of methyl ester
could reach up to 83% with the optimal reaction conditions: oil:
methanol molar ratio of 1: 4, 20% (based on oil) of lipase, initial
water activity of lipase at 0.23 and 20% (based on oil) of tert-butanol
at 40oC after 18 h of reaction time. There was negligible loss in
lipase activity even after repeated use for 30 cycles.