Abstract: This work studied the ability of adipose tissue-derived
mesenchymal stromal cells (MSCs) to form stroma for expansion of
cord blood hematopoietic cells. We showed that 72-hour interaction
of MSCs with cord blood mononuclear cells (MNCs) in vitro at
atmospheric (20%) and low (5%) O2 conditions increased the
expression of ICAM-1, HCAM (at the beginning of interaction) on
MSCs. Viability of MSCs and MNCs were maintained at high level.
Adhesion of MNCs to MSCs was faster at 20% O2. MSCs promoted
the proliferation of adhered MNCs to form the suspension containing
great number of hematopoietic colony-forming units, and this effect
was more pronounced at 5% O2. Thus, adipose-derived MSCs
supplied sufficient stromal support to cord blood MNCs both at 20%
and 5% О2, providing their adhesion with further expansion of new
generation of different hematopoietic lineages.