Abstract: Cryo-electron microscopy (CEM) in combination with
single particle analysis (SPA) is a widely used technique for
elucidating structural details of macromolecular assemblies at closeto-
atomic resolutions. However, development of automated software
for SPA processing is still vital since thousands to millions of
individual particle images need to be processed. Here, we present our
workflow for automated particle picking. Our approach integrates
peak shape analysis to the classical correlation and an iterative
approach to separate macromolecules and background by
classification. This particle selection workflow furthermore provides
a robust means for SPA with little user interaction. Processing
simulated and experimental data assesses performance of the
presented tools.