Abstract: The p53 tumor suppressor gene plays two important
roles in genomic stability: blocking cell proliferation after DNA
damage until it has been repaired, and starting apoptosis if the
damage is too critical. Codon 72 exon4 polymorphism (Arg72Pro) of
the P53 gene has been implicated in cancer risk. Various studies have
been done to investigate the status of p53 at codon 72 for arginine
(Arg) and proline (Pro) alleles in different populations and also the
association of this codon 72 polymorphism with various tumors. Our
objective was to investigate the possible association between P53
Arg72Pro polymorphism and susceptibility to colorectal cancer
among Isfahan and Chaharmahal Va Bakhtiari (a part of south west
of Iran) population. We investigated the status of p53 at codon 72 for
Arg/Arg, Arg/Pro and Pro/Pro allele polymorphisms in blood
samples from 145 colorectal cancer patients and 140 controls by
Nested-PCR of p53 exon 4 and digestion with BstUI restriction
enzyme and the DNA fragments were then resolved by
electrophoresis in 2% agarose gel. The Pro allele was 279 bp, while
the Arg allele was restricted into two fragments of 160 and 119 bp.
Among the 145 colorectal cancer cases 49 cases (33.79%) were
homozygous for the Arg72 allele (Arg/Arg), 18 cases (12.41%) were
homozygous for the Pro72 allele (Pro/Pro) and 78 cases (53.8%)
found in heterozygous (Arg/Pro).
In conclusion, it can be said that p53Arg/Arg genotype may be
correlated with possible increased risk of this kind of cancers in south
west of Iran.
Abstract: The explosive growth of World Wide Web has posed
a challenging problem in extracting relevant data. Traditional web
crawlers focus only on the surface web while the deep web keeps
expanding behind the scene. Deep web pages are created
dynamically as a result of queries posed to specific web databases.
The structure of the deep web pages makes it impossible for
traditional web crawlers to access deep web contents. This paper,
Deep iCrawl, gives a novel and vision-based approach for extracting
data from the deep web. Deep iCrawl splits the process into two
phases. The first phase includes Query analysis and Query translation
and the second covers vision-based extraction of data from the
dynamically created deep web pages. There are several established
approaches for the extraction of deep web pages but the proposed
method aims at overcoming the inherent limitations of the former.
This paper also aims at comparing the data items and presenting them
in the required order.
Abstract: To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
Abstract: Macrobenthos distribution along the coastal waters of
Penang National Park was studid to estimate the effect of different
environmental parameters at three stations, during six sampling
months, from June 2010 to April 2011. The aim of this survey was to
investigate different environment stress over soft bottom polychaete
community along Teluk Ketapang and Pantai Acheh (Penang
National Park) over a year period. Variations in the polychaete
community were evaluated using univariate and multivariate
methods. A total of 604 individuals were examined which was
grouped into 23 families. Family Nereidae was the most abundant
(22.68%), followed by Spionidae (22.02%), Hesionidae (12.58%),
Nephtylidae (9.27%) and Orbiniidae (8.61%). It is noticeable that
good results can only be obtained on the basis of good taxonomic
resolution. The maximum Shannon-Wiener diversity (H'=2.16) was
recorded at distance 200m and 1200m (August 2010) in Teluk
Ketapang and lowest value of diversity was found at distance 1200m
(December 2010) in Teluk Ketapang.