Adherence of Alveolar Fibroblasts and Microorganisms on Titanium Implants
An implant elicits a biological response in the
surrounding tissue which determines the acceptance and long-term
function of the implant. Dental implants have become one of the
main therapy methods in clinic after teeth lose. A successful implant
is in contact with bone and soft tissue represent by fibroblasts. In our
study we focused on the interaction between six different chemically
and physically modified titanium implants (Tis-MALP, Tis-O, Tis-
OA, Tis-OPAAE, Tis-OZ, Tis-OPAE) with alveolar fibroblasts as
well as with five type of microorganisms (S. epidermis, S.mutans, S.
gordonii, S. intermedius, C.albicans). The analysis of microorganism
adhesion was determined by CFU (colony forming unite) and biofilm
formation. The presence of α3β1 and vinculin expression on alveolar
fibroblasts was demonstrated using phospho specific cell based
ELISA (PACE). Alveolar fibroblasts have the highest expression of
these proteins on Tis-OPAAE and Tis-OPAE. It corresponds with
results from bacterial adhesion and biofilm formation and it was
related to the lowest production of collagen I by alveolar fibroblasts
on Tis-OPAAE titanium disc.
[1] B. H. Zhao, W. M. Tian, H .L. Feng, I. S. Lee and F. Z. Cui, "Current
effects of RGD peptide grafting to titanium dental implants on the
adhesion of human gingival fibroblasts and epithelial cells," in Appl
Phys, 2005, vol.5, pp.407-410.
[2] B. Lia, W.Sunb and Y. Jic, "The effect of Nifedipine on the expression
of type I collagen in gingival fibroblasts," JNMU, 2008, vol.22, pp.92-
95.
[3] A. Surazynskia, P. Sienkiewicza, S. Wolczynskib and J. Palka,
"Differential effect of echistatin and trombin on collagen production and
prolidase activity in human dermal fibroblasts and their possible
implication in 1-integrin-mediated signaling," Pharmacol Res, 2005,
vol.51, pp.217-221.
[4] P.R. Kramera, A.J: Keitha, Z. Caib, S. Maa and I. Watanabeb, "Integrin
mediated attachment of periodontal ligament to titanium surfaces," Dent
Mat, 2009, vol.25, pp.877-883.
[5] L. Montanaroa, D. Campocciaa, S Rizzia, M. E. Donatia, L. Breschic, C.
Pratic and C.R. Arciolaa, "Evaluation of bacterial adhesion of
Streptococus mutans on dental restorative material," Biomaterials, 2004,
vol.25, pp.4457-4463.
[6] A. Jabbour, M. Srebnik, B. Zaks, V. Dembitsky and D. Steinberg,
"Evaluation of oxazaborolidine activity on streptococcus mutans biofilm
formation," Int J Antimicrob Agents, 2005, vol.26, pp.491-496.
[1] B. H. Zhao, W. M. Tian, H .L. Feng, I. S. Lee and F. Z. Cui, "Current
effects of RGD peptide grafting to titanium dental implants on the
adhesion of human gingival fibroblasts and epithelial cells," in Appl
Phys, 2005, vol.5, pp.407-410.
[2] B. Lia, W.Sunb and Y. Jic, "The effect of Nifedipine on the expression
of type I collagen in gingival fibroblasts," JNMU, 2008, vol.22, pp.92-
95.
[3] A. Surazynskia, P. Sienkiewicza, S. Wolczynskib and J. Palka,
"Differential effect of echistatin and trombin on collagen production and
prolidase activity in human dermal fibroblasts and their possible
implication in 1-integrin-mediated signaling," Pharmacol Res, 2005,
vol.51, pp.217-221.
[4] P.R. Kramera, A.J: Keitha, Z. Caib, S. Maa and I. Watanabeb, "Integrin
mediated attachment of periodontal ligament to titanium surfaces," Dent
Mat, 2009, vol.25, pp.877-883.
[5] L. Montanaroa, D. Campocciaa, S Rizzia, M. E. Donatia, L. Breschic, C.
Pratic and C.R. Arciolaa, "Evaluation of bacterial adhesion of
Streptococus mutans on dental restorative material," Biomaterials, 2004,
vol.25, pp.4457-4463.
[6] A. Jabbour, M. Srebnik, B. Zaks, V. Dembitsky and D. Steinberg,
"Evaluation of oxazaborolidine activity on streptococcus mutans biofilm
formation," Int J Antimicrob Agents, 2005, vol.26, pp.491-496.
@article{"International Journal of Medical, Medicine and Health Sciences:56508", author = "J. Franková and V. Pivodová and F. Růžička and J. Ulrichová", title = "Adherence of Alveolar Fibroblasts and Microorganisms on Titanium Implants", abstract = "An implant elicits a biological response in the
surrounding tissue which determines the acceptance and long-term
function of the implant. Dental implants have become one of the
main therapy methods in clinic after teeth lose. A successful implant
is in contact with bone and soft tissue represent by fibroblasts. In our
study we focused on the interaction between six different chemically
and physically modified titanium implants (Tis-MALP, Tis-O, Tis-
OA, Tis-OPAAE, Tis-OZ, Tis-OPAE) with alveolar fibroblasts as
well as with five type of microorganisms (S. epidermis, S.mutans, S.
gordonii, S. intermedius, C.albicans). The analysis of microorganism
adhesion was determined by CFU (colony forming unite) and biofilm
formation. The presence of α3β1 and vinculin expression on alveolar
fibroblasts was demonstrated using phospho specific cell based
ELISA (PACE). Alveolar fibroblasts have the highest expression of
these proteins on Tis-OPAAE and Tis-OPAE. It corresponds with
results from bacterial adhesion and biofilm formation and it was
related to the lowest production of collagen I by alveolar fibroblasts
on Tis-OPAAE titanium disc.", keywords = "titanium disc, alveolar fibroblasts, microorganisms,adhesion", volume = "4", number = "4", pages = "134-5", }