Abstract: Xanthan gum is one of the major commercial
biopolymers. Due to its excellent rheological properties xanthan gum
is used in many applications, mainly in food industry. Commercial
production of xanthan gum uses glucose as the carbon substrate;
consequently the price of xanthan production is high. One of the
ways to decrease xanthan price, is using cheaper substrate like
agricultural wastes. Iran is one of the biggest date producer countries.
However approximately 50% of date production is wasted annually.
The goal of this study is to produce xanthan gum from waste date
using Xanthomonas campestris PTCC1473 by submerged
fermentation. In this study the effect of three variables including
phosphor and nitrogen amount and agitation rate in three levels using
response surface methodology (RSM) has been studied. Results
achieved from statistical analysis Design Expert 7.0.0 software
showed that xanthan increased with increasing level of phosphor.
Low level of nitrogen leaded to higher xanthan production. Xanthan
amount, increasing agitation had positive influence. The statistical
model identified the optimum conditions nitrogen amount=3.15g/l,
phosphor amount=5.03 g/l and agitation=394.8 rpm for xanthan. To
model validation, experiments in optimum conditions for xanthan
gum were carried out. The mean of result for xanthan was 6.72±0.26.
The result was closed to the predicted value by using RSM.
Abstract: Removal of a reactive dye (Reactive blue 4) by
adsorption utilizing waste aluminium hydroxide sludge as an
adsorbent was investigated. The removal of the dye was optimized
using response surface methodology (RSM). In the RSM
experiments; initial dye concentration, adsorbent concentration and
contact time were critical parameters. RSM experiments were
performed at the range of initial dye concentration 31.82-368.18
mg/L, adsorbent concentration 3.18-36.82 g/L, contact time 15.82-
56.18 h. Optimum initial dye concentration, adsorbent concentration
and contact time were obtained as 108.83 mg/L, 29.36 g/L and 33.57
h respectively. At these conditions, maximum removal of the dye was
obtained as 95%. The experiments were performed at the optimum
conditions to verify these results and the same results were obtained.
Abstract: The present work is concerned with the effect of turning process parameters (cutting speed, feed rate, and depth of cut) and distance from the center of work piece as input variables on the chip micro-hardness as response or output. Three experiments were conducted; they were used to investigate the chip micro-hardness behavior at diameter of work piece for 30[mm], 40[mm], and 50[mm]. Response surface methodology (R.S.M) is used to determine and present the cause and effect of the relationship between true mean response and input control variables influencing the response as a two or three dimensional hyper surface. R.S.M has been used for designing a three factor with five level central composite rotatable factors design in order to construct statistical models capable of accurate prediction of responses. The results obtained showed that the application of R.S.M can predict the effect of machining parameters on chip micro-hardness. The five level factorial designs can be employed easily for developing statistical models to predict chip micro-hardness by controllable machining parameters. Results obtained showed that the combined effect of cutting speed at it?s lower level, feed rate and depth of cut at their higher values, and larger work piece diameter can result increasing chi micro-hardness.
Abstract: Enzymatic hydrolysis of starch from natural sources
finds potential application in commercial production of alcoholic
beverage and bioethanol. In this study the effect of starch
concentration, temperature, time and enzyme concentration were
studied and optimized for hydrolysis of cassava (Manihot esculenta)
starch powder (of mesh 80/120) into glucose syrup by immobilized
(using Polyacrylamide gel) a-amylase using central composite
design. The experimental result on enzymatic hydrolysis of cassava
starch was subjected to multiple linear regression analysis using
MINITAB 14 software. Positive linear effect of starch concentration,
enzyme concentration and time was observed on hydrolysis of
cassava starch by a-amylase. The statistical significance of the model
was validated by F-test for analysis of variance (p < 0.01). The
optimum value of starch concentration temperature, time and enzyme
concentration were found to be 4.5% (w/v), 45oC, 150 min, and 1%
(w/v) enzyme. The maximum glucose yield at optimum condition
was 5.17 mg/mL.
Abstract: Burnishing is a method of finishing and hardening
machined parts by plastic deformation of the surface. Experimental
work based on central composite second order rotatable design has
been carried out on a lathe machine to establish the effects of ball
burnishing parameters on the surface roughness of brass material.
Analysis of the results by the analysis of variance technique and the
F-test show that the parameters considered, have significant effects
on the surface roughness.
Abstract: The response surface methodology (RSM) is a
collection of mathematical and statistical techniques useful in the
modeling and analysis of problems in which the dependent variable
receives the influence of several independent variables, in order to
determine which are the conditions under which should operate these
variables to optimize a production process. The RSM estimated a
regression model of first order, and sets the search direction using the
method of maximum / minimum slope up / down MMS U/D.
However, this method selects the step size intuitively, which can
affect the efficiency of the RSM. This paper assesses how the step
size affects the efficiency of this methodology. The numerical
examples are carried out through Monte Carlo experiments,
evaluating three response variables: efficiency gain function, the
optimum distance and the number of iterations. The results in the
simulation experiments showed that in response variables efficiency
and gain function at the optimum distance were not affected by the
step size, while the number of iterations is found that the efficiency if
it is affected by the size of the step and function type of test used.
Abstract: Multiprocessor task scheduling is a NP-hard problem and Genetic Algorithm (GA) has been revealed as an excellent technique for finding an optimal solution. In the past, several methods have been considered for the solution of this problem based on GAs. But, all these methods consider single criteria and in the present work, minimization of the bi-criteria multiprocessor task scheduling problem has been considered which includes weighted sum of makespan & total completion time. Efficiency and effectiveness of genetic algorithm can be achieved by optimization of its different parameters such as crossover, mutation, crossover probability, selection function etc. The effects of GA parameters on minimization of bi-criteria fitness function and subsequent setting of parameters have been accomplished by central composite design (CCD) approach of response surface methodology (RSM) of Design of Experiments. The experiments have been performed with different levels of GA parameters and analysis of variance has been performed for significant parameters for minimisation of makespan and total completion time simultaneously.
Abstract: The adsorption of simulated aqueous solution containing textile remazol reactive dye, namely Red 3BS by palm shell activated carbon (PSAC) as adsorbent was carried out using Response Surface Methodology (RSM). A Box-Behnken design in three most important operating variables; initial dye concentration, dosage of adsorbent and speed of impeller was employed for experimental design and optimization of results. The significance of independent variables and their interactions were tested by means of the analysis of variance (ANOVA) with 95% confidence limits. Model indicated that with the increasing of dosage and speed give the result of removal up to 90% with the capacity uptake more than 7 mg/g. High regression coefficient between the variables and the response (R-Sq = 93.9%) showed of good evaluation of experimental data by polynomial regression model.
Abstract: A mathematical model of the surface roughness
has been developed by using response surface methodology
(RSM) in grinding of AISI D2 cold work tool steels. Analysis
of variance (ANOVA) was used to check the validity of the
model. Low and high value for work speed and feed rate are
decided from design of experiment. The influences of all
machining parameters on surface roughness have been
analyzed based on the developed mathematical model. The
developed prediction equation shows that both the feed rate
and work speed are the most important factor that influences
the surface roughness. The surface roughness was found to be
the lowers with the used of low feed rate and low work speed.
Accuracy of the best model was proved with the testing data.
Abstract: Most of the commercial gluten free products are
nutritionally inferior when compared to gluten containing
counterparts as manufacturers most often use the refined flours and
starches. So it is possible that people on gluten free diet have low
intake of fibre content. The foxtail millet flour and copra meal are
gluten free and have high fibre and protein contents. The formulation
of fibre rich gluten free cookies was optimized by response surface
methodology considering independent process variables as proportion
of Foxtail millet (Setaria italica) flour in mixed flour, fat content and
guar gum. The sugar, sodium chloride, sodium bicarbonates and
water were added in fixed proportion as 60, 1.0, 0.4 and 20% of
mixed flour weight, respectively. Optimum formulation obtained for
maximum spread ratio, fibre content, surface L-value, overall
acceptability and minimum breaking strength were 80% foxtail millet
flour in mixed flour, 42.8 % fat content and 0.05% guar gum.
Abstract: This paper investigates the development of weld zone
in Resistance Spot Welding (RSW) which focuses on weld nugget and Heat Affected Zone (HAZ). The effects of four factors namely
weld current, weld time, electrode force and hold time were studied using a general 24 factorial design augmented by five centre points. The results of the analysis showed that all selected factors except
hold time exhibit significant effect on weld nugget radius and HAZ size. Optimization of the welding parameters (weld current, weld
time and electrode force) to normalize weld nugget and to minimize
HAZ size was then conducted using Central Composite Design (CCD) in Response Surface Methodology (RSM) and the optimum
parameters were determined. A regression model for radius of weld nugget and HAZ size was developed and its adequacy was evaluated.
The experimental results obtained under optimum operating conditions were then compared with the predicted values and were
found to agree satisfactorily with each other
Abstract: The present work represents an investigation of the
hydrolysis of hull-less pumpkin (Cucurbita Pepo L.) oil cake protein
isolate (PuOC PI) by pepsin. To examine the effectiveness and
suitability of pepsin towards PuOC PI the kinetic parameters for
pepsin on PuOC PI were determined and then, the hydrolysis process
was studied using Response Surface Methodology (RSM). The
hydrolysis was carried out at temperature of 30°C and pH 3.00. Time
and initial enzyme/substrate ratio (E/S) at three levels were selected
as the independent parameters. The degree of hydrolysis, DH, was
mesuared after 20, 30 and 40 minutes, at initial E/S of 0.7, 1 and 1.3
mA/mg proteins. Since the proposed second-order polynomial model
showed good fit with the experimental data (R2 = 0.9822), the
obtained mathematical model could be used for monitoring the
hydrolysis of PuOC PI by pepsin, under studied experimental
conditions, varying the time and initial E/S. To achieve the highest
value of DH (39.13 %), the obtained optimum conditions for time
and initial E/S were 30 min and 1.024 mA/mg proteins.
Abstract: Response surface methodology (RSM) is a very
efficient tool to provide a good practical insight into developing new
process and optimizing them. This methodology could help
engineers to raise a mathematical model to represent the behavior of
system as a convincing function of process parameters.
Through this paper the sequential nature of the RSM surveyed for process
engineers and its relationship to design of experiments (DOE), regression
analysis and robust design reviewed. The proposed four-step procedure in
two different phases could help system analyst to resolve the parameter
design problem involving responses. In order to check accuracy of the
designed model, residual analysis and prediction error sum of squares
(PRESS) described.
It is believed that the proposed procedure in this study can resolve a
complex parameter design problem with one or more responses. It can be
applied to those areas where there are large data sets and a number of
responses are to be optimized simultaneously. In addition, the proposed
procedure is relatively simple and can be implemented easily by using
ready-made standard statistical packages.
Abstract: Optimization of extraction of phenolic compounds
from Avicennia marina using response surface methodology was
carried out during the present study. Five levels, three factors
rotatable design (CCRD) was utilized to examine the optimum
combination of extraction variables based on the TPC of Avicennia
marina leaves. The best combination of response function was 78.41
°C, drying temperature; 26.18°C; extraction temperature and 36.53
minutes of extraction time. However, the procedure can be promptly
extended to the study of several others pharmaceutical processes like
purification of bioactive substances, drying of extracts and
development of the pharmaceutical dosage forms for the benefit of
consumers.
Abstract: Extraction of lactic acid by emulsion liquid membrane technology (ELM) using n-trioctyl amine (TOA) in n-heptane as carrier within the organic membrane along with sodium carbonate as acceptor phase was optimized by using response surface methodology (RSM). A three level Box-Behnken design was employed for experimental design, analysis of the results and to depict the combined effect of five independent variables, vizlactic acid concentration in aqueous phase (cl), sodium carbonate concentration in stripping phase (cs), carrier concentration in membrane phase (ψ), treat ratio, and batch extraction time (τ)
with equal volume of organic and external aqueous phase on lactic acid extraction efficiency. The maximum lactic acid extraction efficiency (ηext) of 98.21%from aqueous phase in a batch reactor using ELM was found at the optimized values for test variables, cl, cs, ψ, and τ as 0.06 [M], 0.18 [M], 4.72 (%,v/v), 1.98 (v/v) and 13.36 min respectively.
Abstract: Ethanol has been known for a long time, being
perhaps the oldest product obtained through traditional biotechnology
fermentation. Agriculture waste as substrate in fermentation is vastly
discussed as alternative to replace edible food and utilization of
organic material. Pineapple peel, highly potential source as substrate
is a by-product of the pineapple processing industry. Bio-ethanol
from pineapple (Ananas comosus) peel extract was carried out by
controlling fermentation without any treatment. Saccharomyces
ellipsoides was used as inoculum in this fermentation process as it is
naturally found at the pineapple skin. In this study, the capability of
Response Surface Methodology (RSM) for optimization of ethanol
production from pineapple peel extract using Saccharomyces
ellipsoideus in batch fermentation process was investigated. Effect of
five test variables in a defined range of inoculum concentration 6-
14% (v/v), pH (4.0-6.0), sugar concentration (14-22°Brix),
temperature (24-32°C) and time of incubation (30-54 hrs) on the
ethanol production were evaluated. Data obtained from experiment
were analyzed with RSM of MINITAB Software (Version 15)
whereby optimum ethanol concentration of 8.637% (v/v) was
determined. The optimum condition of 14% (v/v) inoculum
concentration, pH 6, 22°Brix, 26°C and 30hours of incubation. The
significant regression equation or model at the 5% level with
correlation value of 99.96% was also obtained.
Abstract: In this study, the sorption of Malachite green (MG) on Hydrilla verticillata biomass, a submerged aquatic plant, was investigated in a batch system. The effects of operating parameters such as temperature, adsorbent dosage, contact time, adsorbent size, and agitation speed on the sorption of Malachite green were analyzed using response surface methodology (RSM). The proposed quadratic model for central composite design (CCD) fitted very well to the experimental data that it could be used to navigate the design space according to ANOVA results. The optimum sorption conditions were determined as temperature - 43.5oC, adsorbent dosage - 0.26g, contact time - 200min, adsorbent size - 0.205mm (65mesh), and agitation speed - 230rpm. The Langmuir and Freundlich isotherm models were applied to the equilibrium data. The maximum monolayer coverage capacity of Hydrilla verticillata biomass for MG was found to be 91.97 mg/g at an initial pH 8.0 indicating that the optimum sorption initial pH. The external and intra particle diffusion models were also applied to sorption data of Hydrilla verticillata biomass with MG, and it was found that both the external diffusion as well as intra particle diffusion contributes to the actual sorption process. The pseudo-second order kinetic model described the MG sorption process with a good fitting.
Abstract: This paper proposes the concept of aerocapture with
aerodynamic-environment-adaptive variable geometry flexible
aeroshell that vehicle deploys. The flexible membrane is composed
of thin-layer film or textile as its aeroshell in order to solve some
problems obstructing realization of aerocapture technique.
Multi-objective optimization study is conducted to investigate
solutions and derive design guidelines. As a result, solutions which
can avoid aerodynamic heating and enlarge the corridor width up
to 10% are obtained successfully, so that the effectiveness of this
concept can be demonstrated. The deformation-use optimum
solution changes its drag coefficient from 1.6 to 1.1, along with the
change in dynamic pressure. Moreover, optimization results show
that deformation-use solution requires the membrane for which
upper temperature limit and strain limit are more than 700 K and
120%, respectively, and elasticity (Young-s modulus) is of order of
106 Pa.
Abstract: Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate concentration, pH, temperature, enzyme concentration on Ethanol Concentration and the above parameters were optimized using Response Surface Methodology (RSM). The optimum values of substrate concentration, pH, temperature and enzyme concentration were found to be 160 g/l, 5.5, 30°C and 50 IU respectively. The effect of inoculums age on ethanol concentration was also investigated. The corn flour solution equivalent to 16% initial starch concentration gave the highest ethanol concentration of 63.04 g/l after 48 h of fermentation at optimum conditions of pH and temperature. Monod model and Logistic model were used for growth kinetics and Leudeking – Piret model was used for product formation kinetics.