Abstract: In this work was developed and implemented a thermal fogging disinfection system to counteract pathogens from poultry feces in agribusiness farms, to reduce mortality rates and increase biosafety in them. The control system consists of two phases for the conditioning of the farm during the sanitary break. In the first phase, viral and bacterial inactivation was performed by treating the stool dry cleaning, along with the development of a specialized product that foster the generation of temperatures above 55 °C in less than 24 hr, for virus inactivation. In the second phase, a process for disinfection by fogging was implemented, along with the development of a specialized disinfectant that guarantee no risk for the operators’ health or birds. As a result of this process, it was possible to minimize the level of mortality of chickens on farms from 12% to 5.49%, representing a reduction of 6.51% in the death rate, through the formula applied to the treatment of poultry litter based on oxidising agents used as antiseptics, hydrogen peroxide solutions, glacial acetic acid and EDTA in order to act on bacteria, viruses, micro bacteria and spores.
Abstract: Vector-borne diseases are transmitted to humans by mosquitos, sandflies, bugs, ticks, and other vectors. Some are re-transmitted between vectors, if the infected human has a new contact when his levels of infection are high. The vector is infected for lifetime and can transmit infectious diseases not only between humans but also from animals to humans. Some vector borne diseases are very disabling and globally account for more than one million deaths worldwide. The mosquitoes from the complex Culex pipiens sl. are the most abundant in Portugal, and we dispose in this moment of a data set from the surveillance program that has been carried on since 2006 across the country. All mosquitos’ species are included, but the large coverage of Culex pipiens sl. and its importance for public health make this vector an interesting candidate to assess risk of disease amplification. This work focus on ports and airports identified as key areas of high density of vectors. Mosquitoes being ectothermic organisms, the main factor for vector survival and pathogen development is temperature. Minima and maxima local air temperatures for each area of interest are averaged by month from data gathered on a daily basis at the national network of meteorological stations, and interpolated in a geographic information system (GIS). The range of temperatures ideal for several pathogens are known and this work shows how to use it with the meteorological data in each port and airport facility, to focus an efficient implementation of countermeasures and reduce simultaneously risk transmission and mitigation costs. The results show an increased alert with decreasing latitude, which corresponds to higher minimum and maximum temperatures and a lower amplitude range of the daily temperature.
Abstract: As computing technology advances, smartphone
applications can assist student learning in a pervasive way. For
example, the idea of using mobile apps for the PA Common Trees,
Pests, Pathogens, in the field as a reference tool allows middle school
students to learn about trees and associated pests/pathogens without
bringing a textbook. While working on the development of three heterogeneous mobile
apps, we ran into numerous challenges. Both the traditional waterfall
model and the more modern agile methodologies failed in practice.
The waterfall model emphasizes the planning of the duration for each
phase. When the duration of each phase is not consistent with the
availability of developers, the waterfall model cannot be employed.
When applying Agile Methodologies, we cannot maintain the high
frequency of the iterative development review process, known as
‘sprints’. In this paper, we discuss the challenges and solutions. We
propose a hybrid model known as the Relay Race Methodology to
reflect the concept of racing and relaying during the process of
software development in practice. Based on the development project,
we observe that the modeling of the relay race transition between any
two phases is manifested naturally. Thus, we claim that the RRM
model can provide a de fecto rather than a de jure basis for the core
concept in the software development model. In this paper, the background of the project is introduced first.
Then, the challenges are pointed out followed by our solutions.
Finally, the experiences learned and the future works are presented.
Abstract: Urinary Tract Infections are considered as one of the
most common bacterial infections with an estimated annual global
incidence of 150 million. Antimicrobial drug resistance is one of the
major threats due to wide spread usage of uncontrolled antibiotics. In
this study, a total number of 9149 urine samples were collected from
R.H Patiala and processed in the Department of Microbiology G. M.
C Patiala (January 2013 to December 2013). Urine samples were
inoculated on MacConkey’s and blood agar plates and incubated at
370C for 24 hrs. The organisms were identified by colony characters,
Gram’s staining, and biochemical reactions. Antimicrobial
susceptibility of the isolates was determined against various
antimicrobial agents (Hi – Media Mumbai India) by Kirby Bauer
DISK diffusion method on Muller Hinton agar plates. Maximum patients were in the age group of 21-30 yrs followed by
31-40 yrs. Males (34%) are less prone to urinary tract infections than
females (66%). Culture was positive in 25% of the samples.
Escherichia coli was the most common isolate 60.3% followed by
Klebsiella pneumoniae 13.5%, Proteus spp. 9% and Staphylococcus
aureus 7.6%. Most of the urinary isolates were sensitive to,
carbepenems, Aztreonam, Amikacin, and Piperacillin + Tazobactum.
All the isolates showed a good sensitivity towards Nitrofurantoin
(82%). ESBL production was found to be 70.6% in Escherichia coli
and 29.4% in Klebsiella pneumonia. Susceptibility of ESBL
producers to Imipenem, Nitrofurantoin and Amikacin were found to
be 100%, 76%, and 75% respectively. Uropathogens are increasingly
showing resistance to many antibiotics making empiric management
of outpatient UTIs challenging. Ampicillin, Cotrimoxazole and
Ciprofloxacin should not be used in empiric treatment. Nitrofurantoin
could be used in lower urinary tract infection. Knowledge of
uropathogens and their antimicrobial susceptibility pattern in a
geographical region will help in appropriate and judicious antibiotic
usage in a health care setup.
Abstract: Food poisoning and infection by bacteria are of public
health significance to both developing and developed countries.
Samples of ogi (akamu) prepared from white and yellow variety of
maize sold in Uturu and Okigwe were analyzed together with the
laboratory prepared ogi for bacterial quality using the standard
microbiological methods. The analyses showed that both white and
yellow variety had total bacterial counts (cfu/g) of 4.0 ×107 and 3.9 x
107 for the laboratory prepared ogi while the commercial ogi had 5.2
x 107 and 4.9 x107, 4.9 x107 and 4.5 x107, 5.4 x107 and 5.0 x107 for
Eke-Okigwe, Up-gate and Nkwo-Achara market respectively. The
Staphylococcal counts ranged from 2.0 x 102 to 5.0 x102 and 1.0 x
102 to 4.0 x102 for the white and yellow variety from the different
markets while Staphylococcal growth was not recorded on the
laboratory prepared ogi. The laboratory prepared ogi had no Coliform
growth while the commercially prepared ogi had counts of 0.5 x103
to 1.6 x 103 for white variety and 0.3 x 103 to 1.1 x103 for yellow
variety respectively. The Lactic acid bacterial count of 3.5x106 and
3.0x106 was recorded for the laboratory ogi while the commercially
prepared ogi ranged from 3.2x106 to 4.2x106 (white variety) and 3.0
x106 to 3.9 x106 (yellow). The presence of bacteria isolates from the
commercial and laboratory fermented ogi showed that Lactobacillus
sp, Leuconostoc sp and Citrobacter sp were present in all the
samples, Micrococcus sp and Klebsiella sp were isolated from Eke-
Okigwe and ABSU-up-gate markets varieties respectively, E. coli
and Staphylococcus sp were present in Eke-Okigwe and Nkwo-
Achara markets while Salmonella sp were isolated from the three
markets. Hence, there are chances of contracting food borne diseases
from commercially prepared ogi. Therefore, there is the need for
sanitary measures in the production of fermented cereals so as to
minimize the rate of food borne pathogens during processing and
storage.
Abstract: The addition of lime as Ca(OH)2 to sewage sludge to
destroy pathogens (Escherichia coli), was evaluated also in relation
to heavy metal bioavailability.
The obtained results show that the use of calcium hydroxide at the
dose of 3% effectively destroyed pathogens ensuring the stability at
high pH values over long period and the duration of the sewage
sludge stabilization. In general, lime addition decreased the total
extractability of heavy metals indicating a reduced bioavailability of
these elements. This is particularly important for a safe utilization in
agricultural soils to reduce the possible transfer of heavy metals to
the food chain.
Abstract: The influences of cell-free solutions (CFSs) of lactic
acid bacteria (LAB) on cadaverine and other biogenic amines
production by Listeria monocytogenes and Staphylococcus aureus
were investigated in lysine decarboxylase broth (LDB) using HPLC.
Cell free solutions were prepared from Lactococcus lactis subsp.
lactis, Leuconostoc mesenteroides subsp. cremoris, Pediococcus
acidilactici and Streptococcus thermophiles. Two different
concentrations that were 50% and 25% CFS and the control without
CFSs were prepared. Significant variations on biogenic amine
production were observed in the presence of L. monocytogenes and S.
aureus (P < 0.05). The function of CFS on biogenic amine production
by foodborne pathogens varied depending on strains and specific
amine. Cadaverine formation by L. monocytogenes and S. aureus in
control were 500.9 and 948.1 mg/L, respectively while the CFSs of
LAB induced 4-fold lower cadaverine production by L.
monocytogenes and 7-fold lower cadaverine production by S. aureus.
The CFSs resulted in strong decreases in cadaverine and putrescine
production by L. monocytogenes and S. aureus, although remarkable
increases were observed for histamine, spermidine, spermine,
serotonin, dopamine, tyramine and agmatine in the presence of LAB
in lysine decarboxylase broth.
Abstract: Since the marine environmental conditions are
extremely different from the other ones, marine actinomycetes might
produce novel bioactive compounds. Therefore, actinomycete strains
were screened from marine water and sediment samples collected
from the coastal areas of Northern Vietnam. Ninety-nine
actinomycete strains were obtained on starch-casein agar media by
dilution technique, only seven strains, named HP112, HP12, HP411,
HPN11, HP 11, HPT13 and HPX12, showed significant antibacterial
activity against both gram-positive and gram-negative bacteria
(Bacillus subtilis ATCC 6633, Staphylococcus epidemidis ATCC
12228, Escherichia coli ATCC 11105). Further studies were carried
out with the most active HP411 strain against Candida albicans
ATCC 10231. This strain could grow rapidly on starch casein agar
and other media with high salt containing 7-10% NaCl at 28-30oC.
Spore-chain of HP411 showed an elongated and circular shape with
10 to 30 spores/chain. Identification of the strain was carried out by
employing the taxonomical studies including the 16S rRNA
sequence. Based on phylogenetic and phenotypic evidence it is
proposed that HP411 to be belongs to species Streptomyces
variabilis. The potent of the crude extract of fermentation broth of
HP411 that are effective against wide range of pathogens: both grampositive,
gram-negative and fungi. Further studies revealed that the
crude extract HP411 could obtain the anticancer activity for cancer
cell lines: Hep-G2 (liver cancer cell line); RD (cardiac and skeletal
muscle letters cell line); FL (membrane of the uterus cancer cell line).
However, the actinomycetes from marine ecosystem will be useful
for the discovery of new drugs in the future.
Abstract: Red River Gum (Eucalyptus camaldulensis) is a tree
of the genus Eucalyptus widely distributed in Algeria and in the
world. The value of its aromatic secondary metabolites offers new
perspectives in the pharmaceutical industry. This strategy can
contribute to the sustainable development of our country. Preliminary
tests performed on the essential oil of Eucalyptus camendulensis
showed that this oil has antibacterial activity vis-à-vis the bacterial
strains (Enterococcus feacalis, Enterobacter cloaceai, Proteus
microsilis, Escherichia coli, Klebsiella pneumonia, and Pseudomonas
aeruginosa) and antifungic (Fusarium sporotrichioide and Fusarium
graminearum). The culture medium used was nutrient broth Muller
Hinton. The interaction between the bacteria and the essential oil is
expressed by a zone of inhibition with diameters of MIC indirectly
expression of. And we used the PDA medium to determine the fungal
activity. The extraction of the aromatic fraction (essentially oilhydrolat)
of the fresh aerian part of the Eucalyptus camendulensis
was performed by hydrodistillation. The average essential oil yield is
0.99%. The antimicrobial and fungal study of the essential oil and
hydrosol showed a high inhibitory effect on the growth of pathogens.
Abstract: Natural antimicrobials are used to preserve foods that
can be found in plants, animals, and microorganisms. Antimicrobial
substances are natural or artificial agents that produced by
microorganisms or obtained semi/total chemical synthesis are used at
low concentrations to inhibit the growth of other microorganisms.
Food borne pathogens and spoilage microorganisms are inactivated
by the use of antagonistic microorganisms and their metabolites.
Yeasts can produce toxic proteins or glycoproteins (toxins) that cause
inhibition of sensitive bacteria and yeast species. Antimicrobial
substance producing phenotypes belonging different yeast genus
were isolated from different sources. Toxins secreted by many yeast
strains inhibiting the growth of other yeast strains. These strains show
antimicrobial activity, inhibiting the growth of mold and bacteria.
The effect of antimicrobial agents produced by yeasts can be
extremely fast, and therefore may be used in various treatment
procedures. Rapid inhibition of microorganisms is possibly caused by
microbial cell membrane lipopolysaccharide binding and in
activation (neutralization) effect. Antimicrobial agents inhibit the
target cells via different mechanisms of action.
Abstract: Endophytic microorganisms are presented in plants of different families growing in the foothills and piedmont plains of Trans-Ili Alatau. It was found that the maximum number of endophytic micromycetes is typical to the Fabaceae family. The number of microscopic fungi in the roots reached (145.9±5.9)×103 CFU/g of plant tissue; yeasts - (79.8±3.5)×102 CFU/g of plant tissue. Basically, endophytic microscopic fungi are typical for underground parts of plants. In contrast, yeasts more infected aboveground parts of plants. Small amount of micromycetes is typical to inflorescence and fruits. Antagonistic activity of selected micromycetes against Fusarium graminearum, Cladosporium sp., Phytophtora infestans and Botrytis cinerea phytopathogens was detected. Strains with a broad, narrow and limited range of action were identified. For further investigations Rh2 and T7 strains were selected, they are characterized by a broad spectrum of fungicidal activity and they formed the large inhibition zones against phytopathogens. Active antagonists are attributed to the Rhodotorula mucilaginosa and Beauveria bassiana species.
Abstract: Mastitis is one of the most economic disease affecting dairy cows worldwide. Its classic diagnosis using bacterial culture and biochemical findings is a difficult and prolonged method. In this research, using of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) permitted identification of different microorganisms with high accuracy and rapidity (only 24 hours for microbial growth and analysis). During the application of MALDI-TOF MS, one hundred twenty strains of Staphylococcus and Streptococcus species isolated from milk of cows affected by clinical and subclinical mastitis were identified, and the results were compared with those obtained by traditional methods as API and VITEK 2 Systems. 37 of totality 39 strains (~95%) of Staphylococcus aureus (S. aureus) were exactly detected by MALDI TOF MS and then confirmed by a nuc-based PCR technique, whereas accurate identification was observed in 100% (50 isolates) of the coagulase negative staphylococci (CNS) and Streptococcus agalactiae (31 isolates). In brief, our results demonstrated that MALDI-TOF MS is a fast and truthful technique which has the capability to replace conventional identification of several bacterial strains usually isolated in clinical laboratories of microbiology.
Abstract: Gold nanoparticles (AuNPs) have gained increasing
interest in recent times. This is greatly due to their special features,
which include unusual optical and electronic properties, high stability
and biological compatibility, controllable morphology and size
dispersion, and easy surface functionalization. In typical synthesis,
AuNPs were produced by reduction of gold salt AuCl4 in an
appropriate solvent. A stabilizing agent was added to prevent the
particles from aggregating. The antibacterial activity of different
sizes of gold nanoparticles was investigated against Staphylococcus
aureus, Salmonella typhi and Pseudomonas pneumonia using the disk
diffusion method in a Müeller–Hinton Agar. The Au-NPs were
effective against all bacteria tested. That the Au-NPs were
successfully synthesized in suspension and were used to study the
antibacterial activity of the two medicinal plants against some
bacterial pathogens suggests that Au-NPs can be employed as an
effective bacteria inhibitor and may be an effective tool in medical
field. The study clearly showed that the Au-NPs exhibiting inhibition
towards the tested pathogenic bacteria in vitro could have the same
effects in vivo and thus may be useful in the medical field if well
researched into.
Abstract: We developed new processes which can collect and detect rapidly airborne pathogens such as the avian flu virus for the pandemic prevention. The fluorescence antibody technique is known as one of high-sensitive detection methods for viruses, but this needs up to a few hours to bind sufficient fluorescence dyes to viruses for detection. In this paper, we developed a mist-labeling can detect substitution viruses in a short time to improve the binding rate of fluorescent dyes and substitution viruses by the micro reaction process. Moreover, we developed the rapid detection system with the above “mist labeling”. The detection system set with a sampling bag collecting patient’s breath and a cartridge can detect automatically pathogens within 10 minutes.
Abstract: The aim of the present work was to test in vitro inhibition of food pathogens and spoilage bacteria by crude bacteriocins from autochthonous lactic acid bacteria. Thirty autochthonous lactic acid bacteria isolated previously, belonging to the genera: Lactobacillus, Carnobacterium, Lactococcus, Vagococcus, Streptococcus, and Pediococcus, have been screened by an agar spot test and a well diffusion assay against Gram-positive and Gram-negative harmful bacteria: Bacillus cereus, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 6538, and Pseudomonas aeruginosa under conditions means to reduce lactic acid and hydrogen peroxide effect to select bacteria with high bacteriocinogenic potential. Furthermore, crude bacteriocins semiquantification and heat sensitivity to different temperatures (80, 95, 110°C, and 121°C) were performed. Another exploratory test concerning the response of St. aureus ATCC 6538 to the presence of crude bacteriocins was realized. It has been observed by the agar spot test that fifteen candidates were active toward Gram-positive targets strains. The secondary screening demonstrated an antagonistic activity oriented only against St. aureus ATCC 6538, leading to the selection of five isolates: Lm14, Lm21, Lm23, Lm24, and Lm25 with a larger inhibition zone compared to the others. The ANOVA statistical analysis reveals a small variation of repeatability: Lm21: 0.56%, Lm23: 0%, Lm25: 1.67%, Lm14: 1.88%, Lm24: 2.14%. Conversely, slight variation was reported in terms of inhibition diameters: 9.58± 0.40, 9.83± 0.46 and 10.16± 0.24 8.5 ± 0.40 10 mm for, Lm21, Lm23, Lm25, Lm14and Lm24, indicating that the observed potential showed a heterogeneous distribution (BMS = 0.383, WMS = 0.117). The repeatability coefficient calculated displayed 7.35%. As for the bacteriocins semiquantification, the five samples exhibited production amounts about 4.16 for Lm21, Lm23, Lm25 and 2.08 AU/ml for Lm14, Lm24. Concerning the sensitivity the crude bacteriocins were fully insensitive to heat inactivation, until 121°C, they preserved the same inhibition diameter. As to, kinetic of growth , the µmax showed reductions in pathogens load for Lm21, Lm23, Lm25, Lm14, Lm24 of about 42.92%, 84.12%, 88.55%, 54.95%, 29.97% in the second trails. Inversely, this pathogen growth after five hours displayed differences of 79.45%, 12.64%, 11.82%, 87.88%, 85.66% in the second trails, compared to the control. This study showed potential inhibition to the growth of this food pathogen, suggesting the possibility to improve the hygienic food quality.
Abstract: Humans use plants for thousands of years to treat various ailments, in many developing countries; much of the population relies on traditional doctors and their collections of medicinal plants to cure them.
Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin.
The aim of our study is to determine the antimicrobial effect of essential oils of the plant Schinus molle on some pathogenic bacteria. It is a medicinal plant used in traditional therapy. Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin.
The test adopted, is based on the diffusion method on solid medium (Antibiogram), this method allows to determine the susceptibility or resistance of an organism according to the sample studied.
Our study reveals that the essential oil of the plant Schinus molle has a different effect on the resistance of germs: for Pseudomonas aeruginosa strain is a moderately sensitive with an inhibition zone of 10mm, further Enterobacter, Escherichia coli and Proteus are strains that represent a high sensitivity, a zone of inhibition equal to 14.66 mm.
Abstract: Changes in the sensory, chemical and microbiological quality of the Mediterranean hake during post-catch handling and distribution were investigated. 115 fish samples were seasonally received during three stages of the transfer route from the sea to the consumer and two storage methods were recorded, seawater and ice storage. Microbiological evaluation revealed higher status for the ice stored samples regarding heterotrophic bacteria (2.68 log cfu/g and 1.92 log cfu/g at 22oC and 37°C respectively) and psychrotrophic counts (3.20 log cfu/g), with statistically significant differences among storage methods. Sensory evaluation also revealed higher status for the ice stored samples with a mean quality index of 0.17 and a spoilage time estimated at 30 hours, in contrast to seawater storage, which varied from 0.28 to 0.3, and a 14-hour estimated spoilage. Detected pathogens were identified mainly in the seawater stored samples, posing questions on the quality of the product reaching the seafood markets.
Abstract: There has been studied the distribution character of potato stem nematode (Ditylenchus destructor Thorne, 1945) on the potato fields in four municipalities (Tsalka, Bolnisi, Marneuli, Gardabani) of Kvemo Kartli (Eastern Georgia).
As a result of scientific research there is stated the extensiveness of pathogens invasion, accompanying composition of fauna species, environmental groups of populations and quantity.
During the research process in the studied ecosystems there were registered 160 forms of free-living and Phyto-parasitic nematodes, from which 118 forms are determined as species and 42 as genus.
It was found that in almost the entire studied ecosystem there is dominated pathogenic nematodes Ditylenchus destructor. The large number of exemplars (almost uncountable) was found in tubers material of Bolnisi and Gardabani.
Abstract: Surgical site infections (SSIs) are the most common
nosocomial infection in surgical patients resulting in significant
increases in postoperative morbidity and mortality. The commonly
causative bacteria developed resistance to virtually all antibiotics
available. The aim of this study was to isolation and identification the
most common bacteria that cause SSIs in Medical Research Institute,
and to compare their sensitivity to selected group of antibiotics and
natural products (garlic, oregano, olive, and Nigella sativa oils). The
isolated pathogens collected from infected surgical wounds were
identified, and their sensitivities to the antibiotics commonly
available for clinical use, and also to the different concentrations of
the used natural products were investigated. The results indicate to
the potential therapeutic effect of the tested natural products in
treatment of surgical wound infections.
Abstract: This research work proposed a study of fruit bruise detection by means of a biospeckle method, selecting the papaya fruit (Carica papaya) as testing body. Papaya is recognized as a fruit of outstanding nutritional qualities, showing high vitamin A content, calcium, carbohydrates, exhibiting high popularity all over the world, considering consumption and acceptability. The commercialization of papaya faces special problems which are associated to bruise generation during harvesting, packing and transportation. Papaya is classified as climacteric fruit, permitting to be harvested before the maturation is completed. However, by one side bruise generation is partially controlled once the fruit flesh exhibits high mechanical firmness. By the other side, mechanical loads can set a future bruise at that maturation stage, when it can not be detected yet by conventional methods. Mechanical damages of fruit skin leave an entrance door to microorganisms and pathogens, which will cause severe losses of quality attributes. Traditional techniques of fruit quality inspection include total soluble solids determination, mechanical firmness tests, visual inspections, which would hardly meet required conditions for a fully automated process. However, the pertinent literature reveals a new method named biospeckle which is based on the laser reflectance and interference phenomenon. The laser biospeckle or dynamic speckle is quantified by means of the Moment of Inertia, named after its mechanical counterpart due to similarity between the defining formulae. Biospeckle techniques are able to quantify biological activities of living tissues, which has been applied to seed viability analysis, vegetable senescence and similar topics. Since the biospeckle techniques can monitor tissue physiology, it could also detect changes in the fruit caused by mechanical damages. The proposed technique holds non invasive character, being able to generate numerical results consistent with an adequate automation. The experimental tests associated to this research work included the selection of papaya fruit at different maturation stages which were submitted to artificial mechanical bruising tests. Damages were visually compared with the frequency maps yielded by the biospeckle technique. Results were considered in close agreement.