Abstract: Purpose: To develop a method for automatic segmentation of adipose and muscular tissue in thighs from magnetic resonance images. Materials and methods: Thirty obese women were scanned on a Siemens Impact Expert 1T resonance machine. 1500 images were finally used in the tests. The developed segmentation method is a recursive and multilevel process that makes use of several concepts such as shaped histograms, adaptative thresholding and connectivity. The segmentation process was implemented in Matlab and operates without the need of any user interaction. The whole set of images were segmented with the developed method. An expert radiologist segmented the same set of images following a manual procedure with the aid of the SliceOmatic software (Tomovision). These constituted our 'goal standard'. Results: The number of coincidental pixels of the automatic and manual segmentation procedures was measured. The average results were above 90 % of success in most of the images. Conclusions: The proposed approach allows effective automatic segmentation of MRIs from thighs, comparable to expert manual performance.
Abstract: Functional gastrointestinal disorders affect millions of people spread all age regardless of race and sex. There are, however, rare diagnostic methods for the functional gastrointestinal disorders because functional disorders show no evidence of organic and physical causes. Our research group identified recently that the gastrointestinal tract well in the patients with the functional gastrointestinal disorders becomes more rigid than healthy people when palpating the abdominal regions overlaying the gastrointestinal tract. Aim of this study is, therefore, to develop a diagnostic system for the functional gastrointestinal disorders based on ultrasound technique, which can quantify the characteristic above related to the rigidity of the gastrointestinal tract well. Ultrasound system was designed. The system consisted of transmitter, ultrasonic transducer, receiver, TGC, and CPLD, and verified via a phantom test. For the phantom test, ten soft-tissue specimens were harvested from porcine. Five of them were then treated chemically to mimic a rigid condition of gastrointestinal tract well, which was induced by functional gastrointestinal disorders. Additionally, the specimens were tested mechanically to identify if the mimic was reasonable. The customized ultrasound system was finally verified through application to human subjects with/without functional gastrointestinal disorders (Normal and Patient Groups). It was identified from the mechanical test that the chemically treated specimens were more rigid than normal specimen. This finding was favorably compared with the result obtained from the phantom test. The phantom test also showed that ultrasound system well described the specimen geometric characteristics and detected an alteration in the specimens. The maximum amplitude of the ultrasonic reflective signal in the rigid specimens (0.2±0.1Vp-p) at the interface between the fat and muscle layers was explicitly higher than that in the normal specimens (0.1±0.0Vp-p). Clinical tests using our customized ultrasound system for human subject showed that the maximum amplitudes of the ultrasonic reflective signals near to the gastrointestinal tract well for the patient group (2.6±0.3Vp-p) were generally higher than those in normal group (0.1±0.2Vp-p). Here, maximum reflective signals was appeared at 20mm depth approximately from abdominal skin for all human subjects, corresponding to the location of the boundary layer close to gastrointestinal tract well. These results suggest that newly designed diagnostic system based on ultrasound technique may diagnose enough the functional gastrointestinal disorders.
Abstract: The human knee joint has a three dimensional
geometry with multiple body articulations that produce complex
mechanical responses under loads that occur in everyday life and
sports activities. To produce the necessary joint compliance and
stability for optimal daily function various menisci and ligaments are
present while muscle forces are used to this effect. Therefore,
knowledge of the complex mechanical interactions of these load
bearing structures is necessary when treatment of relevant diseases is
evaluated and assisting devices are designed.
Numerical tools such as finite element analysis are suitable for
modeling such joints in order to understand their physics. They have
been used in the current study to develop an accurate human knee
joint and model its mechanical behavior. To evaluate the efficacy of
this articulated model, static load cases were used for comparison
purposes with previous experimentally verified modeling works
drawn from literature.
Abstract: Theexperiment was carried out with 2x5 male Merino
lambs raised under intensive conditions to investigate the effect of
dietary calcium soap of linseed oil on the color and fatty acid
composition of longissimusdorsi muscle. Control lambs fed a basal
diet and the experimental lambs consumed a diet supplemented with
3% calcium soap of linseed oil. The color values (L*, a*, b* a*/b*
and chroma) were not influenced by dietary treatment. The MUFA
proportion reduced, SFA and PUFA content did not alter. As
expected, the linolenic (C18:3 n3) and thusthe n-3 content
significantly improved by linseed supplement (0.47 and 0.81; 0.78
and 1.16 in control and in experimental samples, respectively). Other
n-3 and n-6 fatty acids had similar valuestocontrol samples. The n-
6/n-3 ratio was significantly narrower in the experimental group (6.31
vs. 9.38) but the P/S ratio did not differ betweenthe two groups.In
conclusion calcium soap of linseed oil seems to be a suitable
supplement form of n-3 fatty acids to improve the nutritive value of
lamb meat.
Abstract: In a transcutanious inductive coupling of a biomedical
implant, a new formula is given for the study of the Radio Frequency
power attenuation by the biological tissue. The loss of the signal
power is related to its interaction with the biological tissue and the
composition of this one. A confrontation with the practical
measurements done with a synthetic muscle into a Faraday cage,
allowed a checking of the obtained theoretical results. The
supply/data transfer systems used in the case of biomedical implants,
can be well dimensioned by taking in account this type of power
attenuation.
Abstract: The effect of muscle loss due to transfemoral
amputation, on energy expenditure of hip joint and individual
residual muscles was simulated. During swing phase of gait, with
each muscle as an ideal force generator, the lower extremity was
modeled as a two-degree of freedom linkage, for which hip and knee
were joints. According to results, muscle loss will not lead to higher
energy expenditure of hip joint, as long as other parameters of limb
remain unaffected. This finding maybe due to the role of biarticular
muscles in hip and knee joints motion. Moreover, if hip flexors are
removed from the residual limb, residual flexors, and if hip extensors
are removed, residual extensors will do more work. In line with the
common practice in transfemoral amputation, this result demonstrates
during transfemoral amputation, it is important to maintain the length
of residual limb as much as possible.
Abstract: Mathematical models can be used to describe the
dynamics of the spread of infectious disease between susceptibles
and infectious populations. Dengue fever is a re-emerging disease in
the tropical and subtropical regions of the world. Its incidence has
increased fourfold since 1970 and outbreaks are now reported quite
frequently from many parts of the world. In dengue endemic regions,
more cases of dengue infection in pregnancy and infancy are being
found due to the increasing incidence. It has been reported that
dengue infection was vertically transmitted to the infants. Primary
dengue infection is associated with mild to high fever, headache,
muscle pain and skin rash. Immune response includes IgM antibodies
produced by the 5th day of symptoms and persist for 30-60 days. IgG
antibodies appear on the 14th day and persist for life. Secondary
infections often result in high fever and in many cases with
hemorrhagic events and circulatory failure. In the present paper, a
mathematical model is proposed to simulate the succession of dengue
disease transmission in pregnancy and infancy. Stability analysis of
the equilibrium points is carried out and a simulation is given for the
different sets of parameter. Moreover, the bifurcation diagrams of our
model are discussed. The controlling of this disease in infant cases is
introduced in the term of the threshold condition.
Abstract: Atherosclerosis was identified as a chronic inflammatory process resulting from interactions between plasma lipoproteins, cellular components (monocyte, macrophages, T lymphocytes, endothelial cells and smooth muscle cells) and the extracellular matrix of the arterial wall. Several types of genes were known to express during formation of atherosclerosis. This study is carried out to identify unknown differentially expressed gene (DEG) in atherogenesis. Rabbit’s aorta tissues were stained by H&E for histomorphology. GeneFishing™ PCR analysis was performed from total RNA extracted from the aorta tissues. The DNA fragment from DEG was cloned, sequenced and validated by Real-time PCR. Histomorphology showed intimal thickening in the aorta. DEG detected from ACP-41 was identified as cathepsin B gene and showed upregulation at week-8 and week-12 of atherogenesis. Therefore, ACP-based GeneFishing™ PCR facilitated identification of cathepsin B gene which was differentially expressed during development of atherosclerosis.
Abstract: This experiment was conducted to investigate the
effect of different levels of dietary chromium yeast (Cr-yeast) on
thigh meat quality of broiler chicks reared under heat stress
condition. Two hundred and forty Ross male chickens in heat stress
condition (33±3°C) were allocated to five treatments in a completely
randomized design. Treatments were supplemented with 0 (control),
200, 400, 800 and 1200 μg kg-1 Cr in the form of Cr yeast. Twelve
chicks from each treatment were slaughtered at 42 d, to evaluate
moisture, protein, lipid, pH and lipid oxidation of thigh meat.
Protein, moisture, lipid and pH of thigh meat were not affected by
supplemental Cr. Thigh meat lipid tended to decrease in broilers
received 1200 μg kg-1. Storage time increased lipid oxidation of
meat (P
Abstract: To achieve accurate and precise results of finite
element analysis (FEA) of bones, it is important to represent the
load/boundary conditions as identical as possible to the human body
such as the bone properties, the type and force of the muscles, the
contact force of the joints, and the location of the muscle attachment.
In this study, the difference in the Von-Mises stress and the total
deformation was compared by classifying them into Case 1, which
shows the actual anatomical form of the muscle attached to the femur
when the same muscle force was applied, and Case 2, which gives a
simplified representation of the attached location. An inverse
dynamical musculoskeletal model was simulated using data from an
actual walking experiment to complement the accuracy of the
muscular force, the input value of FEA. The FEA method using the
results of the muscular force that were calculated through the
simulation showed that the maximum Von-Mises stress and the
maximum total deformation in Case 2 were underestimated by 8.42%
and 6.29%, respectively, compared to Case 1. The torsion energy and
bending moment at each location of the femur occurred via the stress
ingredient. Due to the geometrical/morphological feature of the femur
of having a long bone shape when the stress distribution is wide, as
shown in Case 1, a greater Von-Mises stress and total deformation are
expected from the sum of the stress ingredients. More accurate results
can be achieved only when the muscular strength and the attachment
location in the FEA of the bones and the attachment form are the same
as those in the actual anatomical condition under the various moving
conditions of the human body.
Abstract: The paper considers a novel modular and intrinsically safe redundant robotic system with biologically inspired actuators (pneumatic artificial muscles and rubber bellows actuators). Similarly to the biological systems, the stiffness of the internal parallel modules, representing 2 DOF joints in the serial robotic chains, is controlled by co-activation of opposing redundant actuator groups in the null-space of the module Jacobian, without influencing the actual robot position. The decoupled position/stiffness control allows the realization of variable joint stiffness according to different force-displacement relationships. The variable joint stiffness, as well as limited pneumatic muscle/bellows force ability, ensures internal system safety that is crucial for development of human-friendly robots intended for human-robot collaboration. The initial experiments with the system prototype demonstrate the capabilities of independently, simultaneously controlling both joint (Cartesian) motion and joint stiffness. The paper also presents the possible industrial applications of snake-like robots built using the new modules.
Abstract: The disaster from functional gastrointestinal disorders has detrimental impact on the quality of life of the effected population and imposes a tremendous social and economic burden. There are, however, rare diagnostic methods for the functional gastrointestinal disorders. Our research group identified recently that the gastrointestinal tract well in the patients with the functional gastrointestinal disorders becomes more rigid than healthy people when palpating the abdominal regions overlaying the gastrointestinal tract. Objective of current study is, therefore, identify feasibility of a diagnostic system for the functional gastrointestinal disorders based on ultrasound technique, which can quantify the characteristics above. Two-dimensional finite difference (FD) models (one normal and two rigid model) were developed to analyze the reflective characteristic (displacement) on each soft-tissue layer responded after application of ultrasound signals. The FD analysis was then based on elastic ultrasound theory. Validation of the model was performed via comparison of the characteristic of the ultrasonic responses predicted by FD analysis with that determined from the actual specimens for the normal and rigid conditions. Based on the results from FD analysis, ultrasound system for diagnosis of the functional gastrointestinal disorders was developed and clinically tested via application of it to 40 human subjects with/without functional gastrointestinal disorders who were assigned to Normal and Patient Groups. The FD models were favorably validated. The results from FD analysis showed that the maximum displacement amplitude in the rigid models (0.12 and 0.16) at the interface between the fat and muscle layers was explicitly less than that in the normal model (0.29). The results from actual specimens showed that the maximum amplitude of the ultrasonic reflective signal in the rigid models (0.2±0.1Vp-p) at the interface between the fat and muscle layers was explicitly higher than that in the normal model (0.1±0.2 Vp-p). Clinical tests using our customized ultrasound system showed that the maximum amplitudes of the ultrasonic reflective signals near to the gastrointestinal tract well for the patient group (2.6±0.3 Vp-p) were generally higher than those in normal group (0.1±0.2 Vp-p). Here, maximum reflective signals was appeared at 20mm depth approximately from abdominal skin for all human subjects, corresponding to the location of the boundary layer close to gastrointestinal tract well. These findings suggest that our customized ultrasound system using the ultrasonic reflective signal may be helpful to the diagnosis of the functional gastrointestinal disorders.
Abstract: This study examined the effects of 8-week Pilates training program on limits of stability (LOS) and abdominal muscle strength in young dancers. Twenty-four female volunteered and randomly assigned as experimental group (EG) or control group (CG). All subjects received the same dance lessons but the EG underwent an extra Pilates mat exercises for 40 minutes, three times a week, for 8 weeks. LOS was evaluated by the Biodex Balance System and the abdominal strength was measured by 30/60 seconds sit-ups test. One factor ANCOVA was used to examine the differences between groups after training. The results showed that the overall LOS scores at levels 2/8 and the 30/60 seconds sit-ups for the EG group pre- and post-training were changed from 22/38 % to 31/51 % and 20/33 times to 24/42 times, respectively. The study demonstrated that 8-week Pilates training can improve the LOS performance and abdominal strength in young dancers.
Abstract: Many studies have emphasized the importance of
resistive exercise to maintain a healthy human body, particular in
prevention of weakening of physical strength. Recently, some studies
advocated that an application of vibration as a supplementary means in
a regular training was effective in encouraging physical strength. Aim
of the current study was, therefore, to identify if an application of
vibration in a resistive exercise was effective in encouraging physical
strength as that in a regular training. A 3-dimensional virtual lower
extremity model for a healthy male and virtual leg-press model were
generated and synchronized. Dynamic leg-press exercises on a slide
machine with/without extra load and on a footboard with vibration as
well as on a slide machine with extra load were analyzed. The results
of the current indicated that the application of the vibration on the
dynamic leg-press exercise might be not greatly effective in
encouraging physical strength, compared with the dynamic leg press
exercise with extra load. It was, however, thought that the application
of the vibration might be helpful to elderly individuals because the
reduced maximum muscle strength appeared by the effect of the
vibration may avoid a muscular spasm, which can be driven from a
high muscle strength sometimes produced during the leg-press
exercise with extra load.
Abstract: Twenty four male Thai native × Anglo-Nubian
crossbred goats were randomly allocated to receive four treatments.
The experiment was conducted for four months and slaughtered that
the Longissimus dorsi muscle was collected for fatty acid analysis.
The results conclude that either castrated method or ages had no
significantly different on monounsaturated fatty acid (MUFA)
(P>0.05) except erucic acid (C22:1n9). Interaction between castrated
method and ages had significantly different in MUFA (P
Abstract: The paper is included within the framework of a
complex research program, which was initiated from the hypothesis
arguing on the existence of a correlation between pineal indolic and
peptide hormones and the somatic development rhythm, including
thus the epithalamium-epiphysis complex involvement. At birds,
pineal gland contains a circadian oscillator, playing a main role in the
temporal organization of the cerebral functions. The secretion of
pineal indolic hormones is characterized by a high endogenous
rhythmic alternation, modulated by the light/darkness (L/D)
succession and by temperature as well. The research has been carried
out using 100 chicken broilers - “Ross" commercial hybrid,
randomly allocated in two experimental batches: Lc batch, reared
under a 12L/12D lighting schedule and Lexp batch, which was photic
pinealectomised through continuous exposition to light (150 lux, 24
hours, 56 days). Chemical and physical features of the meat issued
from breast fillet and thighs muscles have been studied, determining
the dry matter, proteins, fat, collagen, salt content and pH value, as
well. Besides the variations of meat chemical composition in relation
with lighting schedule, other parameters have been studied: live
weight dynamics, feed intake and somatic development degree. The
achieved results became significant since chickens have 7 days of
age, some variations of the studied parameters being registered,
revealing that the pineal gland physiologic activity, in relation with
the lighting schedule, could be interpreted through the monitoring of
the somatic development technological parameters, usually studied
within the chicken broilers rearing aviculture practice.
Abstract: In this study, the effect of L-arginine was examined at the neuromuscular junction of the chick biventer cervicis muscle. LArginine at 500 μg/ ml, decreased twitch response to electerical stimulation, and produced rightward shift of the dose- response curve for acetylcholine or carbachol. L-Arginine at 1000μg/ ml produced a strong shift to the right of the dose – response curve for acetylcholine or carbachol with a reduction in the efficacy. The inhibitory effect of L-arginine on the twitch response was blocked by caffeine (200μg/ ml). NO levels were also measured in the chick biventer cervicis muscle homogenates, using spectrophotometric method for the direct detection of NO, nitrite and nitrate. Total nitrite (nitrite + nitrate) was measured by a spectrophotometer at 540 nm after the conversion of nitrate to nitrite by copperized cadmium granules. NO levels were found to be significantly increased in concentrations 500 and 1000μg/ ml of L-arginine in comparison with the control group (p
Abstract: Electroencephalogram (EEG) recordings are often
contaminated with ocular and muscle artifacts. In this paper, the
canonical correlation analysis (CCA) is used as blind source
separation (BSS) technique (BSS-CCA) to decompose the artifact
contaminated EEG into component signals. We combine the BSSCCA
technique with wavelet filtering approach for minimizing both
ocular and muscle artifacts simultaneously, and refer the proposed
method as wavelet enhanced BSS-CCA. In this approach, after
careful visual inspection, the muscle artifact components are
discarded and ocular artifact components are subjected to wavelet
filtering to retain high frequency cerebral information, and then clean
EEG is reconstructed. The performance of the proposed wavelet
enhanced BSS-CCA method is tested on real EEG recordings
contaminated with ocular and muscle artifacts, for which power
spectral density is used as a quantitative measure. Our results suggest
that the proposed hybrid approach minimizes ocular and muscle
artifacts effectively, minimally affecting underlying cerebral activity
in EEG recordings.
Abstract: “Dengue" is an African word meaning “bone
breaking" because it causes severe joint and muscle pain that feels
like bones are breaking. It is an infectious disease mainly transmitted
by female mosquito, Aedes aegypti, and causes four serotypes of
dengue viruses. In recent years, a dramatic increase in the dengue
fever confirmed cases around the equator-s belt has been reported.
Several conventional indices have been designed so far to monitor the
transmitting vector populations known as House Index (HI),
Container Index (CI), Breteau Index (BI). However, none of them
describes the adult mosquito population size which is important to
direct and guide comprehensive control strategy operations since
number of infected people has a direct relationship with the vector
density. Therefore, it is crucial to know the population size of the
transmitting vector in order to design a suitable and effective control
program. In this context, a study is carried out to report a new
statistical index, ABURAS Index, using Poisson distribution based
on the collection of vector population in Jeddah Governorate, Saudi Arabia.
Abstract: This study aimed to evaluate the muscularity and tissue composition of 24 legs of Ile de France lambs. They were fed with diets containing “in nature" or hydrolyzed sugarcane with 0.6% of calcium oxide in aerobic and anaerobic environments. Animals entered the trial at 15 and were slaughtered at 32 kg of body weight. The leg tissue composition, as well as muscularity (0.47), muscle:bone (6.66) and muscle:fat (4.25) were not affected (P>0.05) by treatments. The proportions found were: 67.62% for muscle, 17.52% for bone and 10.15% for fat. In relation to lambs fed with “in nature" sugarcane, hydrolyzed sugarcane with calcium oxide in aerobic and anaerobic environments did not affect muscularity and leg tissue composition of lambs.