Abstract: In present study the effects of anti-inflammatory and
antinociceptive of vitex hydro-alcoholic extract were evaluated on
male mice. In inflammatory test mice were divided into 7 groups:
first group was control. The second group, positive control group,
received dexamethasone (15 mg/kg) and the other five groups
received different doses of hydroalcohol extract of Vitex fruit (265,
365, 465, 565, and 665 mg/kg). The inflammation was caused by
xylene-induced ear edema. Formalin test was used for evaluation of
antinociceptive effect of extract. In this test, mice were divided into 7
groups: control, morphine (10mg/kg) as positive control group, and
Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs
were administered intrapritoneally, 30 min before each test. The data
were analyzed using one-way ANOVA followed by Tukey-kramer
multiple comparison test. Results have shown significant antiinflammatory
effects of extract at all dosed as compared with control
(P
Abstract: The aims of this paper are to study the efficacy of
chitosan nanoparticles in stimulating specific antibody against
A/H1N1 influenza antigen in mice. Chitosan nanoparticles (CSN)
were characterized by TEM. The results showed that the average size
of CSN was from 80nm to 106nm. The efficacy of A/H1N1 influenza
vaccine loaded on the surface of CSN showed that loading efficiency
of A/H1N1 influenza antigen on CSN was from 93.75 to 100%. Safe
property of the vaccine were tested. In 10 days post vaccination,
group of CSN 30 kDa and 300 kDa loaded A/H1N1 influenza antigen
were the rate of immune response on mice to be 100% (9/9) higher
than Al(OH)3 and other adjuvant. 100% mice in the experiment of all
groups had immune response in 20 days post vaccination. The results
also showed that HI titer of the group using CSN 300 kDa as an
adjuvant increased significantly up to 3971 HIU, over three-fold
higher than the Al(OH)3 adjuvant, chitosan (CS), and one hundredfold
than the A/H1N1 antigen only. Stability of the vaccine
formulation was investigated.
Abstract: This research was carried out to determine the
possible effects of low electromagnetic field (EMF) exposure to the
developing mice fetuses. Pregnant mice were exposed to EMF
exposure at 0mT (sham) and 1.2 mT for six hours per session, carried
out on gestation day 3, 6, 9, 12 and 15. Samples from the stillborn
offspring were observed for morphological defects. The heart didn-t
show progressive cellular damage, the lungs were congested and
emphysemics. The bones were in advance stage of hypertrophy.
Spectrums of morphological defects were observed over 70% of the
surviving offspring. These results indicate that even at lower
exposure to low EMF, is enough to induce morphological defects in
prenatal mice.