Abstract: Interaction of Schiff base complexes of Iron and
Manganese: Iron [N, N’ Bis (5- (triphenyl phosphonium methyl)
salicylidene) -1, 2 ethanediamine) chloride, [Fe Salen]Cl; Manganese
[N, N’ Bis (5- (triphenyl phosphonium methyl) salicylidene) -1, 2
ethanediamine) acetate, were investigated by spectroscopic and
isothermal titration calorimetry techniques (ITC).
The absorbance spectra of complexes have shown hyper and
hypochromism in the presence of DNA that is indication of
interaction of complexes with DNA. The linear dichroism (LD)
measurements confirmed the bending of DNA in the presence of
complexes.
Furthermore, Isothermal titration calorimetry experiments
approved that complexes bound to DNA on the base of both
electrostatic and hydrophobic interactions. More, ITC profile exhibits
the existence of two binding phases for the complexes. Antibacterial
activity of ligand and complexes were tested in vitro to evaluate their
activity against the gram positive and negative bacteria.
Abstract: Array-based gene expression analysis is a powerful
tool to profile expression of genes and to generate information on
therapeutic effects of new anti-cancer compounds. Anti-apoptotic
effect of thymoquinone was studied in MCF7 breast cancer cell line
using gene expression profiling with cDNA microarray. The purity
and yield of RNA samples were determined using RNeasyPlus Mini
kit. The Agilent RNA 6000 NanoLabChip kit evaluated the quantity
of the RNA samples. AffinityScript RT oligo-dT promoter primer
was used to generate cDNA strands. T7 RNA polymerase was used to
convert cDNA to cRNA. The cRNA samples and human universal
reference RNA were labelled with Cy-3-CTP and Cy-5-CTP,
respectively. Feature Extraction and GeneSpring softwares analysed
the data. The single experiment analysis revealed involvement of 64
pathways with up-regulated genes and 78 pathways with downregulated
genes. The MAPK and p38-MAPK pathways were
inhibited due to the up-regulation of PTPRR gene. The inhibition of
p38-MAPK suggested up-regulation of TGF-ß pathway. Inhibition of
p38-MAPK caused up-regulation of TP53 and down-regulation of
Bcl2 genes indicating involvement of intrinsic apoptotic pathway.
Down-regulation of CARD16 gene as an adaptor molecule regulated
CASP1 and suggested necrosis-like programmed cell death and
involvement of caspase in apoptosis. Furthermore, down-regulation
of GPCR, EGF-EGFR signalling pathways suggested reduction of
ER. Involvement of AhR pathway which control cytochrome P450
and glucuronidation pathways showed metabolism of Thymoquinone.
The findings showed differential expression of several genes in
apoptosis pathways with thymoquinone treatment in estrogen
receptor-positive breast cancer cells.
Abstract: Enterococci are important inhabitants of the animal
intestine and are widely used in probiotic products. A probiotic strain
is expected to possess several desirable properties in order to exert
beneficial effects. Therefore, the objective of this study was to
isolate, characterize and identify Enterococcus sp. from chicken cecal
and fecal samples to determine potential probiotic properties.
Enterococci were isolated from chicken ceca and feces of thirty three
clinically healthy chickens from a local farm. In vitro studies were
performed to assess antibacterial activity of the isolated LAB (using
agar well diffusion and cell free supernatant broth technique against
Salmonella enterica serotype Enteritidis), survival in acidic
conditions, resistance to bile salts, and their survival during simulated
gastric juice conditions at pH 2.5. Isolates were identified by
biochemical carbohydrate fermentation patterns using an API 50
CHL kit and API ZYM kits and by sequenced 16S rDNA. An isolate
belonging to E. faecium species exhibited inhibitory effect against S.
enteritidis. This isolate producing a clear zone as large as 10.30 mm
or greater and was able to grow in the coculture medium and at the
same time, inhibited the growth S. enteritidis. In addition, E. faecium
exhibited significant resistance under highly acidic conditions at pH
2.5 for 8 h and survived well in bile salt at 0.2% for 24 h and showing
ability to survive in the presence of simulated gastric juice at pH 2.5.
Based on these results, E. faecium isolate fulfills some of the criteria
to be considered as a probiotic strain and therefore, could be used as a
feed additive with good potential for controlling S. Enteritidis in
chickens. However, in vivo studies are needed to determine the safety
of the strain.
Abstract: This paper examines international marketing in
business practice of Czech exporting small and medium-sized
enterprises (SMEs) with regard to the strategic perspectives.
Research was focused on Czech exporting SMEs from Moravian-
Silesia region and their behavior on international markets. For
purpose of collecting data, a questionnaire was given to 262 SMEs
involved in international business. Statistics utilized in this research
included frequency, mean, percentage, and chi-square test. Data were
analyzed by Statistical Package for the Social Sciences software. The
research analysis disclosed that there is certain space for
improvement in strategic marketing especially in a marketing
research, perception of cultural and social differences, product
adaptation and usage of marketing communication tools.
Abstract: The present work analyses different parameters of end
milling to minimize the surface roughness for AISI D2 steel. D2 Steel
is generally used for stamping or forming dies, punches, forming
rolls, knives, slitters, shear blades, tools, scrap choppers, tyre
shredders etc. Surface roughness is one of the main indices that
determines the quality of machined products and is influenced by
various cutting parameters. In machining operations, achieving
desired surface quality by optimization of machining parameters, is a
challenging job. In case of mating components the surface roughness
become more essential and is influenced by the cutting parameters,
because, these quality structures are highly correlated and are
expected to be influenced directly or indirectly by the direct effect of
process parameters or their interactive effects (i.e. on process
environment). In this work, the effects of selected process parameters
on surface roughness and subsequent setting of parameters with the
levels have been accomplished by Taguchi’s parameter design
approach. The experiments have been performed as per the
combination of levels of different process parameters suggested by
L9 orthogonal array. Experimental investigation of the end milling of
AISI D2 steel with carbide tool by varying feed, speed and depth of
cut and the surface roughness has been measured using surface
roughness tester. Analyses of variance have been performed for mean
and signal-to-noise ratio to estimate the contribution of the different
process parameters on the process.
Abstract: Doxorubicin, also known as Adriamycin, is an
anthracycline class of drug used in cancer chemotherapy. It is used in
the treatment of non-Hodgkin’s lymphoma, multiple myeloma, acute
leukemia, breast cancer, lung cancer, endometrium cancer and ovary
cancers. It functions via intercalating DNA and ultimately killing
cancer cells. The major side effects of doxorubicin are hair loss,
myelosuppression, nausea & vomiting, oesophagitis, diarrhea, heart
damage and liver dysfunction. The minor modifications in the
structure of compound exhibit large variation in the biological
activity, has prompted us to carry out the synthesis of sulfonamide
derivatives. Sulfonamide is an important feature with broad spectrum
of biological activity such as antiviral, antifungal, diuretics, antiinflammatory,
antibacterial and anticancer activities. Structure of the
synthesized compound N-(1-methyl-2-oxo-2-N-methyl anilinoethyl)
benzene sulfonamide confirmed by proton nuclear magnetic
resonance (1H NMR),13C NMR, Mass and FTIR spectroscopic tools
to assure the position of all protons and hence stereochemistry of the
molecule. Further we have reported the binding potential of
synthesized sulfonamide analogues in comparison to doxorubicin
drug using Auto Dock 4.2 software. Computational binding energy
(B.E.) and inhibitory constant (Ki) has been evaluated for the
synthesized compound in comparison of doxorubicin against Poly
(dA-dT).Poly (dA-dT) and Poly (dG-dC).Poly (dG-dC) sequences.
The in vitro cytotoxic study against human breast cancer cell lines
confirms the better anticancer activity of the synthesized compound
over currently in use anticancer drug doxorubicin. The IC50 value of
the synthesized compound is 7.12 μM whereas for doxorubicin is 7.2
μM.
Abstract: For centuries humans have used the antimicrobial
properties of copper to their advantage. Yet, after all these years the
underlying mechanisms of copper mediated cell death in various
microbes remain unclear. We had explored the hypothesis that copper
mediated increased levels of lipid peroxidation in the membrane fatty
acids is responsible for increased killing in Escherichia coli. In this
study we show that in both gram positive (Staphylococcus aureus)
and gram negative (Pseudomonas aeruginosa) bacteria there is a
strong correlation between copper mediated cell death and increased
levels of lipid peroxidation. Interestingly, the non-spore forming
gram positive bacteria as well as gram negative bacteria show similar
patterns of cell death, increased levels of lipid peroxidation, as well
as genomic DNA degradation, however there is some difference in
loss in membrane integrity upon exposure to copper alloy surface.
Abstract: Agriculture is the backbone of economy of Pakistan
and cotton is the major agricultural export and supreme source of raw
fiber for our textile industry. To combat severe problems of insect
and weed, combination of three genes namely Cry1Ac, Cry2A and
EPSPS genes was transferred in locally cultivated cotton variety
MNH-786 with the use of Agrobacterium mediated genetic
transformation. The present study focused on the molecular screening
of transgenic cotton plants at T3 generation in order to confirm
integration and expression of all three genes (Cry1Ac, Cry2A and
EPSP synthase) into the cotton genome. Initially, glyphosate spray
assay was used for screening of transgenic cotton plants containing
EPSP synthase gene at T3 generation. Transgenic cotton plants which
were healthy and showed no damage on leaves were selected after 07
days of spray. For molecular analysis of transgenic cotton plants in
the laboratory, the genomic DNA of these transgenic cotton plants
were isolated and subjected to amplification of the three genes. Thus,
seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A
gene) and all twenty (EPSP synthase gene) were produced positive
amplification. On the base of PCR amplification, ten transgenic plant
samples were subjected to protein expression analysis through
ELISA. The results showed that eight out of ten plants were actively
expressing the three transgenes. Real-time PCR was also done to
quantify the mRNA expression levels of Cry1Ac and EPSP synthase
gene. Finally, eight plants were confirmed for the presence and active
expression of all three genes at T3 generation.
Abstract: Although, arsenic trioxide has been the subject of
toxicological research, in vitro cytotoxicity and genotoxicity studies
using relevant cell models and uniform methodology are not well
elucidated. Hence, the aim of the present study was to evaluate the
cytotoxicity and genotoxicity induced by arsenic trioxide in human
keratinocytes (HaCaT) using the MTT [3-(4, 5-dimethylthiazol-2-yl)-
2,5-diphenyltetrazolium bromide] and alkaline single cell gel
electrophoresis (Comet) assays, respectively. Human keratinocytes
were treated with different doses of arsenic trioxide for 4 h prior to
cytogenetic assessment. Data obtained from the MTT assay indicated
that arsenic trioxide significantly reduced the viability of HaCaT cells
in a dose-dependent manner, showing an IC50 value of 34.18 ± 0.6
μM. Data generated from the comet assay also indicated a significant
dose-dependent increase in DNA damage in HaCaT cells associated
with arsenic trioxide exposure. We observed a significant increase in
comet tail length and tail moment, showing an evidence of arsenic
trioxide -induced genotoxic damage in HaCaT cells. This study
confirms that the comet assay is a sensitive and effective method to
detect DNA damage caused by arsenic.
Abstract: Analyzing DNA microarray data sets is a great
challenge, which faces the bioinformaticians due to the complication
of using statistical and machine learning techniques. The challenge
will be doubled if the microarray data sets contain missing data,
which happens regularly because these techniques cannot deal with
missing data. One of the most important data analysis process on
the microarray data set is feature selection. This process finds the
most important genes that affect certain disease. In this paper, we
introduce a technique for imputing the missing data in microarray
data sets while performing feature selection.
Abstract: Domestic goats (Capra hircus) are extremely diverse
species and principal animal genetic resource of the developing
world. These facilitate a persistent supply of meat, milk, fibre, and
skin and are considered as important revenue generators in small
pastoral environments. This study aimed to fingerprint β-LG gene at
PCR-RFLP level in native Saudi goat breeds (Ardi, Habsi and Harri)
in an attempt to have a preliminary image of β-LG genotypic patterns
in Saudi breeds as compared to other foreign breeds such as Indian
and Egyptian. Also, the Phylogenetic analysis was done to investigate
evolutionary trends and similarities among the caprine β-LG gene
with that of the other domestic specie, viz. cow, buffalo and sheep.
Blood samples were collected from 300 animals (100 for each breed)
and genomic DNA was extracted. A fragment of the β-LG gene
(427bp) was amplified using specific primers. Subsequent digestion
with Sac II restriction endonuclease revealed two alleles (A and B)
and three different banding patterns or genotypes i.e. AA, AB and
BB. The statistical analysis showed a general trend that β-LG AA
genotype had higher milk yield than β-LG AB and β-LG BB
genotypes. Nucleotide sequencing of the selected β-LG fragments
was done and submitted to GenBank NCBI (Accession No.
KJ544248, KJ588275, KJ588276, KJ783455, KJ783456 and
KJ874959). Phylogenetic analysis on the basis of nucleotide
sequences of native Saudi goats indicated evolutional similarity with
the GenBank reference sequences of goat, Bubalus bubalis and Bos
taurus. However, the origin of sheep which is the most closely
related from the evolutionary point of view, was located some
distance away.
Abstract: This paper is concerning the issues of behaviour of
lightweight expanded clay aggregates concrete exposed to high
temperature. Lightweight aggregates from expanded clay are
produced by firing of row material up to temperature 1050°C.
Lightweight aggregates have suitable properties in terms of volume
stability, when exposed to temperatures up to 1050°C, which could
indicate their suitability for construction applications with higher risk
of fire. The test samples were exposed to heat by using the standard
temperature-time curve ISO 834. Negative changes in resulting
mechanical properties, such as compressive strength, tensile strength,
and flexural strength were evaluated. Also visual evaluation of the
specimen was performed. On specimen exposed to excessive heat, an
explosive spalling could be observed, due to evaporation of
considerable amount of unbounded water from the inner structure of
the concrete.
Abstract: Persea declinata (Bl.) Kosterm is a member of the
Lauraceae family, widely distributed in Southeast Asia. It is from the
same genus with avocado (Persea americana Mill), which is widely
consumed as food and for medicinal purposes. In the present study,
we examined the anticancer properties of Persea declinata (Bl.)
Kosterm bark methanolic crude extract (PDM). PDM exhibited a
potent antiproliferative effect in MCF-7 human breast cancer cells,
with an IC50 value of 16.68 .g/mL after 48h of treatment. We
observed that PDM caused cell cycle arrest and subsequent apoptosis
in MCF-7 cells, as exhibited by increased population at G0/G1 phase,
higher lactate dehydrogenase (LDH) release, and DNA
fragmentation. Mechanistic studies showed that PDM caused
significant elevation in ROS production, leading to perturbation of
mitochondrial membrane potential, cell permeability, and activation
of caspases-3/7. On the other hand, real-time PCR and Western blot
analysis showed that PDM treatment increased the expression of the
proapoptotic molecule, Bax, but decreased the expression of
prosurvival proteins, Bcl-2 and Bcl-xL, in a dose-dependent manner.
These findings imply that PDM could inhibit proliferation in MCF-7
cells via cell cycle arrest and apoptosis induction, indicating its
potential as a therapeutic agent worthy of further development.
Abstract: Pollution of the Klip River has caused
microorganisms inhabiting it to develop protective survival
mechanisms. This study isolated and characterized the heavy metal
resistant bacteria in the Klip River. Water and sediment samples were
collected from six sites along the course of the river. The pH,
turbidity, salinity, temperature and dissolved oxygen were measured
in-situ. The concentrations of six heavy metals (Cd, Cu, Fe, Ni, Pb
and Zn) of the water samples were determined by atomic absorption
spectroscopy. Biochemical and antibiotic profiles of the isolates were
assessed using the API 20E® and Kirby Bauer Method. Growth
studies were carried out using spectrophotometric methods. The
isolates were identified using 16SrDNA sequencing. The uppermost
part of the Klip River with the lowest pH had the highest levels of
heavy metals. Turbidity, salinity and specific conductivity increased
measurably at Site 4 (Henley on Klip Weir). MIC tests showed that
16 isolates exhibited high iron and lead resistance. Antibiotic
susceptibility tests revealed that the isolates exhibited multitolerances
to drugs such as Tetracycline, Ampicillin, and
Amoxicillin.
Abstract: Frequent pattern mining is the process of finding a
pattern (a set of items, subsequences, substructures, etc.) that occurs
frequently in a data set. It was proposed in the context of frequent
itemsets and association rule mining. Frequent pattern mining is used
to find inherent regularities in data. What products were often
purchased together? Its applications include basket data analysis,
cross-marketing, catalog design, sale campaign analysis, Web log
(click stream) analysis, and DNA sequence analysis. However, one of
the bottlenecks of frequent itemset mining is that as the data increase
the amount of time and resources required to mining the data
increases at an exponential rate. In this investigation a new algorithm
is proposed which can be uses as a pre-processor for frequent itemset
mining. FASTER (FeAture SelecTion using Entropy and Rough sets)
is a hybrid pre-processor algorithm which utilizes entropy and roughsets
to carry out record reduction and feature (attribute) selection
respectively. FASTER for frequent itemset mining can produce a
speed up of 3.1 times when compared to original algorithm while
maintaining an accuracy of 71%.
Abstract: This paper presents a new meta-heuristic bio-inspired
optimization algorithm which is called Cuttlefish Algorithm (CFA).
The algorithm mimics the mechanism of color changing behavior of
the cuttlefish to solve numerical global optimization problems. The
colors and patterns of the cuttlefish are produced by reflected light
from three different layers of cells. The proposed algorithm considers
mainly two processes: reflection and visibility. Reflection process
simulates light reflection mechanism used by these layers, while
visibility process simulates visibility of matching patterns of the
cuttlefish. To show the effectiveness of the algorithm, it is tested with
some other popular bio-inspired optimization algorithms such as
Genetic Algorithms (GA), Particle Swarm Optimization (PSO) and
Bees Algorithm (BA) that have been previously proposed in the
literature. Simulations and obtained results indicate that the proposed
CFA is superior when compared with these algorithms.
Abstract: In this study, six bacterial isolates of a slightly
thermophilic organism from the Sg. Klah hot spring, Malaysia were
successfully isolated and designated as M7T55D1, M7T55D2,
M7T55D3, M7T53D1, M7T53D2 and M7T53D3 respectively. The
bacterial isolates were screened for their cellulose hydrolytic ability
on Carboxymethlycellulose agar medium. The isolated bacterial
strains were identified morphologically, biochemically and
molecularly with the aid of 16S rDNA sequencing. All of the bacteria
showed their optimum growth at a slightly alkaline pH of 7.5 with a
temperature of 55°C. All strains were Gram-negative, non-spore
forming type, strictly aerobic, catalase-positive and oxidase-positive
with the ability to produce thermostable cellulase. Based on BLASTn
results, bacterial isolates of M7T55D2 and M7T53D1 gave the
highest homology (97%) with similarity to Tepidimonas ignava while
isolates M7T55D1, M7T55D3, M7T53D2 and M7T53D3 showed
their closest homology (97%-98%) with Tepidimonas thermarum.
These cellulolytic thermophiles might have a commercial potential to
produce valuable thermostable cellulase.
Abstract: Virtual reality (VR) is a rapidly emerging computer
interface that attempts to immerse the user completely within an
experimental recreation; thereby, greatly enhancing the overall
impact and providing a much more intuitive link between the
computer and the human participants. The main objective of this
study is to design tractor trailer capable of meeting the customers’
requirements and suitable for rough conditions to be used in
combination with a farm tractor in India. The final concept is capable
of providing arrangements for attaching the trailer to the tractor easily
by pickup hitch, stronger and lighter supporting frame, option of
spare tyre etc. Furthermore, the resulting product design can be sent
via the Internet to customers for comments or marketing purposes.
The virtual prototyping (VP) system therefore facilitates advanced
product design and helps reduce product development time and cost
significantly.
Abstract: The purpose of this study is to identify the teaching method practices of the practical work subject in Vocational Secondary School. This study examined the practice of Vocational Teaching Method in Automotive Practical Work. The quantitative method used the sets of the questionnaire. 283 students and 63 teachers involved from ten VSS involved in this research. Research finding showed in conducting the introduction session teachers prefer used the demonstration method and questioning technique. While in deliver the content of practical task, teachers applied group monitoring and problem solving approach. To conclude the task of automotive practical work, teachers choose re-explain and report writing to make sure students really understand all the process of teaching. VTM-APW also involved the competency-based concept to embed in the model. Derived from factors investigated, research produced the combination of elements in teaching skills and vocational skills which could be used as the best teaching method in automotive practical work for school level. As conclusion this study has concluded that the VTM-APW model is able to apply in teaching to make an improvement with current practices in Vocational Secondary School. Hence, teachers are suggested to use this method to enhance student's knowledge in Automotive and teachers will deliver skills to the current and future workforce relevant with the required competency skilled in workplace.
Abstract: Two new metal-based anticancer chemotherapeutic
agents, [(Ph2Sn)2(HGuO)2(phen)Cl2] 1 and [(Ph3Sn)(HGuO)(phen)]-
Cl.CH3OH.H2O 2, were designed, prepared and characterized by
analytical and spectral (IR, ESI-Mass, 1H, 13C and 119Sn NMR)
techniques. The proposed geometry of Sn(IV) in 1 and 2 is distorted
octahedral and distorted trigonal-bipyramidal, respectively. Both 1
and 2 exhibit potential cytotoxicity in vitro against MCF-7, HepG-2
and DU-145 cell lines. The intrinsic binding constant (Kb) values of 1
(2.33 × 105 M-1) and 2 (2.46 × 105 M-1) evaluated from UV-Visible
absorption studies suggest non-classical electrostatic mode of
interaction via phosphate backbone of DNA double helix. The Stern-
Volmer quenching constant (Ksv) of 1 (9.74 × 105 M-1) and 2 (2.9 ×
106 M-1) determined by fluorescence studies suggests the groove
binding and intercalation mode for 1 and 2, respectively. Effective
cleavage of pBR322 DNA is induced by 1.Their interaction with
DNA of cancer cells may account for potency.