Abstract: D-erythro-cyclohexylserine (D
chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion
system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene
was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on
genomic sequence of Shinorhizobium meliloti
Sequence analysis of the cloned DNA fragment revealed one
open-reading frame of 1059 bp and 386 amino acids. This putative
D-TA gene was cloned into NdeI and EcoRI (pEnsi
His-tag sequence or BamHI (pEnsi-DTA[2])
sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with
pEnsi-DTA[2]. When the cells expressing the wild
used for D-TA enzyme activity, 12 mM glycine was successfully
detected in HPLC analysis. Moreover, the whole cells harbouring the
recombinant D-TA was able to synthesize D-erythro
of 0.6 mg/ml in a batch reaction.
Abstract: Access control is a critical security service in Wire- less
Sensor Networks (WSNs). To prevent malicious nodes from joining
the sensor network, access control is required. On one hand, WSN
must be able to authorize and grant users the right to access to the
network. On the other hand, WSN must organize data collected by
sensors in such a way that an unauthorized entity (the adversary)
cannot make arbitrary queries. This restricts the network access only
to eligible users and sensor nodes, while queries from outsiders will
not be answered or forwarded by nodes. In this paper we presentee
different access control schemes so as to ?nd out their objectives,
provision, communication complexity, limits, etc. Using the node
density parameter, we also provide a comparison of these proposed
access control algorithms based on the network topology which can
be flat or hierarchical.
Abstract: European Union candidate status provides a
strong motivation for decision-making in the candidate
countries in shaping the regional development policy where
there is an envisioned transfer of power from center to the
periphery. The process of Europeanization anticipates the
candidate countries configure their regional institutional
templates in the context of the requirements of the European
Union policies and introduces new instruments of incentive
framework of enlargement to be employed in regional
development schemes. It is observed that the contribution of
the local actors to the decision making in the design of the
allocation architectures enhances the efficiency of the funds
and increases the positive effects of the projects funded under
the regional development objectives. This study aims at
exploring the performances of the three regional development
grant schemes in Turkey, established and allocated under the
pre-accession process with a special emphasis given to the
roles of the national and local actors in decision-making for
regional development. Efficiency analyses have been
conducted using the DEA methodology which has proved to
be a superior method in comparative efficiency and
benchmarking measurements. The findings of this study as
parallel to similar international studies, provides that the
participation of the local actors to the decision-making in
funding contributes both to the quality and the efficiency of
the projects funded under the EU schemes.
Abstract: This research contribution is drafted to present the
orbit design, orbit propagator and geomagnetic field estimator for the
nanosatellites specifically for the upcoming CUBESAT, ICUBE-1 of
the Institute of Space Technology (IST), Islamabad, Pakistan. The
ICUBE mission is designed for the low earth orbit at the approximate
height of 700KM. The presented research endeavor designs the
Keplarian elements for ICUBE-1 orbit while incorporating the
mission requirements and propagates the orbit using J2 perturbations,
The attitude determination system of the ICUBE-1 consists of
attitude determination sensors like magnetometer and sun sensor. The
Geomagnetic field estimator is developed according to the model of
International Geomagnetic Reference Field (IGRF) for comparing the
magnetic field measurements by the magnetometer for attitude
determination. The output of the propagator namely the Keplarians
position and velocity vectors and the magnetic field vectors are
compared and verified with the same scenario generated in the
Satellite Tool Kit (STK).
Abstract: Fixed-point simulation results are used for the
performance measure of inverting matrices by Cholesky
decomposition. The fixed-point Cholesky decomposition algorithm
is implemented using a fixed-point reconfigurable processing
element. The reconfigurable processing element provides all
mathematical operations required by Cholesky decomposition. The
fixed-point word length analysis is based on simulations using
different condition numbers and different matrix sizes. Simulation
results show that 16 bits word length gives sufficient performance
for small matrices with low condition number. Larger matrices and
higher condition numbers require more dynamic range for a fixedpoint
implementation.
Abstract: Since straightness error of linear motor stage is hardly
dependent upon machining accuracy and assembling accuracy, there is
limit on maximum realizable accuracy. To cope with this limitation,
this paper proposed a servo system to compensate straightness error of
a linear motor stage. The servo system is mounted on the slider of the
linear motor stage and moves in the direction of the straightness error
so as to compensate the error. From position dependency and
repeatability of the straightness error of the slider, a feedforward
compensation control is applied to the platform servo control. In the
consideration of required fine positioning accuracy, a platform driven
by an electro-magnetic actuator is suggested and a sliding mode
control was applied. The effectiveness of the sliding mode control was
verified along with some experimental results.
Abstract: In this paper is investigated a possible
optimization of some linear algebra problems which can be
solved by parallel processing using the special arrays called
systolic arrays. In this paper are used some special types of
transformations for the designing of these arrays. We show
the characteristics of these arrays. The main focus is on
discussing the advantages of these arrays in parallel
computation of matrix product, with special approach to the
designing of systolic array for matrix multiplication.
Multiplication of large matrices requires a lot of
computational time and its complexity is O(n3 ). There are
developed many algorithms (both sequential and parallel) with
the purpose of minimizing the time of calculations. Systolic
arrays are good suited for this purpose. In this paper we show
that using an appropriate transformation implicates in finding
more optimal arrays for doing the calculations of this type.
Abstract: In this paper the reference current for Voltage Source
Converter (VSC) of the Shunt Active Power Filter (SAPF) is
generated using Synchronous Reference Frame method,
incorporating the PI controller with anti-windup scheme. The
proposed method improves the harmonic filtering by compensating
the winding up phenomenon caused by the integral term of the PI
controller.
Using Reference Frame Transformation, the current is transformed
from om a - b - c stationery frame to rotating 0 - d - q frame. Using
the PI controller, the current in the 0 - d - q frame is controlled to
get the desired reference signal. A controller with integral action
combined with an actuator that becomes saturated can give some
undesirable effects. If the control error is so large that the integrator
saturates the actuator, the feedback path becomes ineffective because
the actuator will remain saturated even if the process output changes.
The integrator being an unstable system may then integrate to a very
large value, the phenomenon known as integrator windup.
Implementing the integrator anti-windup circuit turns off the
integrator action when the actuator saturates, hence improving the
performance of the SAPF and dynamically compensating harmonics
in the power network. In this paper the system performance is
examined with Shunt Active Power Filter simulation model.
Abstract: Adhesively bonded joints are preferred over the
conventional methods of joining such as riveting, welding, bolting
and soldering. Some of the main advantages of adhesive joints
compared to conventional joints are the ability to join dissimilar
materials and damage-sensitive materials, better stress distribution,
weight reduction, fabrication of complicated shapes, excellent
thermal and insulation properties, vibration response and enhanced
damping control, smoother aerodynamic surfaces and an
improvement in corrosion and fatigue resistance. This paper presents
the behavior of adhesively bonded joints subjected to combined
thermal loadings, using the numerical methods. The joint
configuration considers aluminum as central adherend with six
different outer adherends including aluminum, steel, titanium, boronepoxy,
unidirectional graphite-epoxy and cross-ply graphite-epoxy
and epoxy-based adhesives. Free expansion of the joint in x
direction was permitted and stresses in adhesive layer and interfaces
calculated for different adherends.
Abstract: Recently there has been a growing interest in the field
of bio-mimetic robots that resemble the behaviors of an insect or an
aquatic animal, among many others. One of various bio-mimetic robot
applications is to explore pipelines, spotting any troubled areas or
malfunctions and reporting its data. Moreover, the robot is able to
prepare for and react to any abnormal routes in the pipeline. Special
types of mobile robots are necessary for the pipeline monitoring tasks.
In order to move effectively along a pipeline, the robot-s movement
will resemble that of insects or crawling animals. When situated in
massive pipelines with complex routes, the robot places fixed sensors
in several important spots in order to complete its monitoring. This
monitoring task is to prevent a major system failure by preemptively
recognizing any minor or partial malfunctions. Areas uncovered by
fixed sensors are usually impossible to provide real-time observation
and examination, and thus are dependent on periodical offline
monitoring. This paper proposes a monitoring system that is able to
monitor the entire area of pipelines–with and without fixed
sensors–by using the bio-mimetic robot.
Abstract: Recent trends in building constructions in Libya are
more toward tall (high-rise) building projects. As a consequence, a
better estimation of the lateral loading in the design process is
becoming the focal of a safe and cost effective building industry. Byin-
large, Libya is not considered a potential earthquake prone zone,
making wind is the dominant design lateral loads. Current design
practice in the country estimates wind speeds on a mere random
bases by considering certain factor of safety to the chosen wind
speed. Therefore, a need for a more accurate estimation of wind
speeds in Libya was the motivation behind this study. Records of
wind speed data were collected from 22 metrological stations in
Libya, and were statistically analysed. The analysis of more than four
decades of wind speed records suggests that the country can be
divided into four zones of distinct wind speeds. A computer “survey"
program was manipulated to draw design wind speeds contour map
for the state of Libya.
The paper presents the statistical analysis of Libya-s recorded
wind speed data and proposes design wind speed values for a 50-year
return period that covers the entire country.
Abstract: In this paper, we propose a face recognition algorithm
using AAM and Gabor features. Gabor feature vectors which are well
known to be robust with respect to small variations of shape, scaling,
rotation, distortion, illumination and poses in images are popularly
employed for feature vectors for many object detection and
recognition algorithms. EBGM, which is prominent among face
recognition algorithms employing Gabor feature vectors, requires
localization of facial feature points where Gabor feature vectors are
extracted. However, localization method employed in EBGM is based
on Gabor jet similarity and is sensitive to initial values. Wrong
localization of facial feature points affects face recognition rate. AAM
is known to be successfully applied to localization of facial feature
points. In this paper, we devise a facial feature point localization
method which first roughly estimate facial feature points using AAM
and refine facial feature points using Gabor jet similarity-based facial
feature localization method with initial points set by the rough facial
feature points obtained from AAM, and propose a face recognition
algorithm using the devised localization method for facial feature
localization and Gabor feature vectors. It is observed through
experiments that such a cascaded localization method based on both
AAM and Gabor jet similarity is more robust than the localization
method based on only Gabor jet similarity. Also, it is shown that the
proposed face recognition algorithm using this devised localization
method and Gabor feature vectors performs better than the
conventional face recognition algorithm using Gabor jet
similarity-based localization method and Gabor feature vectors like
EBGM.
Abstract: The so-called all-pass filter circuits are commonly
used in the field of signal processing, control and measurement.
Being connected to capacitive loads, these circuits tend to loose their
stability; therefore the elaborate analysis of their dynamic behavior is
necessary. The compensation methods intending to increase the
stability of such circuits are discussed in this paper, including the socalled
lead-lag compensation technique being treated in detail. For
the dynamic modeling, a two-port network model of the all-pass filter
is being derived. The results of the model analysis show, that
effective lead-lag compensation can be achieved, alone by the
optimization of the circuit parameters; therefore the application of
additional electric components are not needed to fulfill the stability
requirement.
Abstract: A state of the art Speaker Identification (SI) system requires a robust feature extraction unit followed by a speaker modeling scheme for generalized representation of these features. Over the years, Mel-Frequency Cepstral Coefficients (MFCC) modeled on the human auditory system has been used as a standard acoustic feature set for SI applications. However, due to the structure of its filter bank, it captures vocal tract characteristics more effectively in the lower frequency regions. This paper proposes a new set of features using a complementary filter bank structure which improves distinguishability of speaker specific cues present in the higher frequency zone. Unlike high level features that are difficult to extract, the proposed feature set involves little computational burden during the extraction process. When combined with MFCC via a parallel implementation of speaker models, the proposed feature set outperforms baseline MFCC significantly. This proposition is validated by experiments conducted on two different kinds of public databases namely YOHO (microphone speech) and POLYCOST (telephone speech) with Gaussian Mixture Models (GMM) as a Classifier for various model orders.
Abstract: Most routing protocols (DSR, AODV etc.) that have
been designed for wireless adhoc networks incorporate the broadcasting
operation in their route discovery scheme. Probabilistic broadcasting
techniques have been developed to optimize the broadcast operation
which is otherwise very expensive in terms of the redundancy
and the traffic it generates. In this paper we have explored percolation
theory to gain a different perspective on probabilistic broadcasting
schemes which have been actively researched in the recent years.
This theory has helped us estimate the value of broadcast probability
in a wireless adhoc network as a function of the size of the network.
We also show that, operating at those optimal values of broadcast
probability there is at least 25-30% reduction in packet regeneration
during successful broadcasting.
Abstract: The purpose of this study is to present a non invasive
method for the marginal adaptation evaluation in class V composite
restorations. Standardized class V cavities, prepared in human
extracted teeth, were filled with Premise (Kerr) composite. The
specimens were thermo cycled. The interfaces were examined by
Optical Coherence Tomography method (OCT) combined with the
confocal microscopy and fluorescence. The optical configuration
uses two single mode directional couplers with a superluminiscent
diode as the source at 1300 nm. The scanning procedure is similar to
that used in any confocal microscope, where the fast scanning is enface
(line rate) and the depth scanning is much slower (at the frame
rate). Gaps at the interfaces as well as inside the composite resin
materials were identified. OCT has numerous advantages which
justify its use in vivo as well as in vitro in comparison with
conventional techniques.
Abstract: The response surface methodology (RSM) is a
collection of mathematical and statistical techniques useful in the
modeling and analysis of problems in which the dependent variable
receives the influence of several independent variables, in order to
determine which are the conditions under which should operate these
variables to optimize a production process. The RSM estimated a
regression model of first order, and sets the search direction using the
method of maximum / minimum slope up / down MMS U/D.
However, this method selects the step size intuitively, which can
affect the efficiency of the RSM. This paper assesses how the step
size affects the efficiency of this methodology. The numerical
examples are carried out through Monte Carlo experiments,
evaluating three response variables: efficiency gain function, the
optimum distance and the number of iterations. The results in the
simulation experiments showed that in response variables efficiency
and gain function at the optimum distance were not affected by the
step size, while the number of iterations is found that the efficiency if
it is affected by the size of the step and function type of test used.
Abstract: Non-saturated soils that while saturation greatly
decrease their volume, have sudden settlement due to increasing
humidity, fracture and structural crack are called loess soils. Whereas
importance of civil projects including: dams, canals and
constructions bearing this type of soil and thereof problems, it is
required for carrying out more research and study in relation to loess
soils. This research studies shear strength parameters by using
grading test, Atterberg limit, compression, direct shear and
consolidation and then effect of using cement and lime additives on
stability of loess soils is studied. In related tests, lime and cement are
separately added to mixed ratios under different percentages of soil
and for different times the stabilized samples are processed and effect
of aforesaid additives on shear strength parameters of soil is studied.
Results show that upon passing time the effect of additives and
collapsible potential is greatly decreased and upon increasing
percentage of cement and lime the maximum dry density is
decreased; however, optimum humidity is increased. In addition,
liquid limit and plastic index is decreased; however, plastic index
limit is increased. It is to be noted that results of direct shear test
reveal increasing shear strength of soil due to increasing cohesion
parameter and soil friction angle.
Abstract: Transaction management is one of the most crucial requirements for enterprise application development which often require concurrent access to distributed data shared amongst multiple application / nodes. Transactions guarantee the consistency of data records when multiple users or processes perform concurrent operations. Existing Fault Tolerance Infrastructure for Mobile Agents (FTIMA) provides a fault tolerant behavior in distributed transactions and uses multi-agent system for distributed transaction and processing. In the existing FTIMA architecture, data flows through the network and contains personal, private or confidential information. In banking transactions a minor change in the transaction can cause a great loss to the user. In this paper we have modified FTIMA architecture to ensure that the user request reaches the destination server securely and without any change. We have used triple DES for encryption/ decryption and MD5 algorithm for validity of message.
Abstract: The challenge for software development house in
Bangladesh is to find a path of using minimum process rather than CMMI or ISO type gigantic practice and process area. The small and medium size organization in Bangladesh wants to ensure minimum
basic Software Process Improvement (SPI) in day to day operational
activities. Perhaps, the basic practices will ensure to realize their company's improvement goals. This paper focuses on the key issues in basic software practices for small and medium size software
organizations, who are unable to effort the CMMI, ISO, ITIL etc. compliance certifications. This research also suggests a basic software process practices model for Bangladesh and it will show the mapping of our suggestions with international best practice. In this IT
competitive world for software process improvement, Small and medium size software companies that require collaboration and
strengthening to transform their current perspective into inseparable global IT scenario. This research performed some investigations and analysis on some projects- life cycle, current good practice, effective approach, reality and pain area of practitioners, etc. We did some
reasoning, root cause analysis, comparative analysis of various
approach, method, practice and justifications of CMMI and real life. We did avoid reinventing the wheel, where our focus is for minimal
practice, which will ensure a dignified satisfaction between
organizations and software customer.