The Effects of TiO2 Nanoparticles on Tumor Cell Colonies: Fractal Dimension and Morphological Properties

Semiconductor nanomaterials like TiO2 nanoparticles (TiO2-NPs) approximately less than 100 nm in diameter have become a new generation of advanced materials due to their novel and interesting optical, dielectric, and photo-catalytic properties. With the increasing use of NPs in commerce, to date few studies have investigated the toxicological and environmental effects of NPs. Motivated by the importance of TiO2-NPs that may contribute to the cancer research field especially from the treatment prospective together with the fractal analysis technique, we have investigated the effect of TiO2-NPs on colony morphology in the dark condition using fractal dimension as a key morphological characterization parameter. The aim of this work is mainly to investigate the cytotoxic effects of TiO2-NPs in the dark on the growth of human cervical carcinoma (HeLa) cell colonies from morphological aspect. The in vitro studies were carried out together with the image processing technique and fractal analysis. It was found that, these colonies were abnormal in shape and size. Moreover, the size of the control colonies appeared to be larger than those of the treated group. The mean Df +/- SEM of the colonies in untreated cultures was 1.085±0.019, N= 25, while that of the cultures treated with TiO2-NPs was 1.287±0.045. It was found that the circularity of the control group (0.401±0.071) is higher than that of the treated group (0.103±0.042). The same tendency was found in the diameter parameters which are 1161.30±219.56 μm and 852.28±206.50 μm for the control and treated group respectively. Possible explanation of the results was discussed, though more works need to be done in terms of the for mechanism aspects. Finally, our results indicate that fractal dimension can serve as a useful feature, by itself or in conjunction with other shape features, in the classification of cancer colonies.

The Comparative Analysis of Two Typical Fluidic Thrust Vectoring Exhaust Nozzles on Aerodynamic Characteristics

The comparisons of two typical fluidic thrust vectoring exhaust nozzles including two-dimensional(2-D) nozzle and axisymmetric nozzle on aerodynamic characteristics was presented by numerical simulation. The results show: the thrust vector angles increased with the increasing secondary flow but decreased with the nozzle pressure ratio (NPR) increasing. With the same secondary flow and NPR, the thrust vector angles of 2-D nozzle were higher than the axisymmetric nozzle-s. So with the lower NPR and more secondary weight flow, the much higher thrust vector angle was caused by 2-D fluidic nozzle. And with the higher NPR and less secondary weight flow, there was not much difference in angular dimension between two nozzles.

GSM-Based Approach for Indoor Localization

Ability of accurate and reliable location estimation in indoor environment is the key issue in developing great number of context aware applications and Location Based Services (LBS). Today, the most viable solution for localization is the Received Signal Strength (RSS) fingerprinting based approach using wireless local area network (WLAN). This paper presents two RSS fingerprinting based approaches – first we employ widely used WLAN based positioning as a reference system and then investigate the possibility of using GSM signals for positioning. To compare them, we developed a positioning system in real world environment, where realistic RSS measurements were collected. Multi-Layer Perceptron (MLP) neural network was used as the approximation function that maps RSS fingerprints and locations. Experimental results indicate advantage of WLAN based approach in the sense of lower localization error compared to GSM based approach, but GSM signal coverage by far outreaches WLAN coverage and for some LBS services requiring less precise accuracy our results indicate that GSM positioning can also be a viable solution.

Effect of Different Treatments on the Periphyton Quantity and Quality in Experimental Fishponds

Periphyton development and composition were studied in three different treatments: (i) two fishpond units of wetland-type wastewater treatment pond systems, (ii) two fishponds in combined intensive-extensive fish farming systems and (iii) three traditional polyculture fishponds. Results showed that amounts of periphyton developed in traditional polyculture fishponds (iii) were different compared to the other treatments (i and ii), where the main function of ponds was stated wastewater treatment. Negative correlation was also observable between water quality parameters and periphyton production. The lower trophity, halobity and saprobity level of ponds indicated higher amount of periphyton. The dry matter content of periphyton was significantly higher in the samples, which were developed in traditional polyculture fishponds (2.84±3.02 g m-2 day-1, whereby the ash content in dry matter 74%), than samples taken from (i) (1.60±2.32 g m-2 day-1, 61%) and (ii) fishponds (0.65±0.45 g m-2 day-1, 81%).

Analysis of Normal Penetration of Ogive -Nose Projectiles into Thin Metallic Plates

In this note, a theoretical model for analyzing of normal penetration of the ogive – nose projectile into metallic targets is presented .The failure is assumed to be asymmetry petalling and the analysis is performed by using the energy balance and work done .The work done consist of the work required for plastic deformation Wp, the work for transferring the matter to new position Wd and the work for bending of the petals Wb. In several studies, it has been shown that we can neglect the loss of energy by temperature. In this present study, in first, by assuming the crater formation after perforation, the value of work done is calculated during the normal penetration of conical projectiles into thin metallic targets. Then the value of residual velocity and ballistic limit of the projectile is predicated by using the energy balance. In final, theoretical and experimental results is compared.

Performance Analysis of Routing Protocol for WSN Using Data Centric Approach

Sensor Network are emerging as a new tool for important application in diverse fields like military surveillance, habitat monitoring, weather, home electrical appliances and others. Technically, sensor network nodes are limited in respect to energy supply, computational capacity and communication bandwidth. In order to prolong the lifetime of the sensor nodes, designing efficient routing protocol is very critical. In this paper, we illustrate the existing routing protocol for wireless sensor network using data centric approach and present performance analysis of these protocols. The paper focuses in the performance analysis of specific protocol namely Directed Diffusion and SPIN. This analysis reveals that the energy usage is important features which need to be taken into consideration while designing routing protocol for wireless sensor network.

WiPoD Wireless Positioning System based on 802.11 WLAN Infrastructure

This paper describes WiPoD (Wireless Position Detector) which is a pure software based location determination and tracking (positioning) system. It uses empirical signal strength measurements from different wireless access points for mobile user positioning. It is designed to determine the location of users having 802.11 enabled mobile devices in an 802.11 WLAN infrastructure and track them in real time. WiPoD is the first main module in our LBS (Location Based Services) framework. We tested K-Nearest Neighbor and Triangulation algorithms to estimate the position of a mobile user. We also give the analysis results of these algorithms for real time operations. In this paper, we propose a supportable, i.e. understandable, maintainable, scalable and portable wireless positioning system architecture for an LBS framework. The WiPoD software has a multithreaded structure and was designed and implemented with paying attention to supportability features and real-time constraints and using object oriented design principles. We also describe the real-time software design issues of a wireless positioning system which will be part of an LBS framework.

Real Time Approach for Data Placement in Wireless Sensor Networks

The issue of real-time and reliable report delivery is extremely important for taking effective decision in a real world mission critical Wireless Sensor Network (WSN) based application. The sensor data behaves differently in many ways from the data in traditional databases. WSNs need a mechanism to register, process queries, and disseminate data. In this paper we propose an architectural framework for data placement and management. We propose a reliable and real time approach for data placement and achieving data integrity using self organized sensor clusters. Instead of storing information in individual cluster heads as suggested in some protocols, in our architecture we suggest storing of information of all clusters within a cell in the corresponding base station. For data dissemination and action in the wireless sensor network we propose to use Action and Relay Stations (ARS). To reduce average energy dissipation of sensor nodes, the data is sent to the nearest ARS rather than base station. We have designed our architecture in such a way so as to achieve greater energy savings, enhanced availability and reliability.

Effect of Cyclotron Resonance Frequencies in Particles Due to AC and DC Electromagnetic Fields

A fundamental model consisting of charged particles moving in free space exposed to alternating and direct current (ACDC) electromagnetic fields is analyzed. Effects of charged particles initial position and initial velocity to cyclotron resonance frequency are observed. Strong effects are observed revealing that effects of electric and magnetic fields on a charged particle in free space varies with the initial conditions. This indicates the frequency where maximum displacement occur can be changed. At this frequency the amplitude of oscillation of the particle displacement becomes unbounded.

Mechanical Behaviour and Electrical Conductivity of Oxygen Separation Membrane under Uniaxial Compressive Loading

The mechanical deformation and the electrical conductivity of lanthanum strontium cobalt ferrite oxide under uniaxial compression were investigated at various temperatures up to 1073 K. The material reveals a rather complex mechanical behaviour related to its ferroelasticity and completely different stress-strain curves are obtained during the 1st and 2nd loading cycles. A distinctive ferroelastic creep was observed at 293 K whilst typical ferroelastic stress-strain curve were obtained in the temperature range from 473 K to 873 K. At 1073 K, on the other hand, high-temperature creep deformation was observed instead of ferroelastic deformation. The conductivity increases with increasing compressive stress at all the temperatures. The increase in conductivity is related to both geometrical and piezoelectric effects. From 293 K to 873 K, where the material exhibits ferroelastic behaviour, the variation in the total conductivity decreases with increasing temperature. The contribution of the piezoelectric effect to the total conductivity variation also decreases with increasing temperature and the maximum in piezoconductivity has a value of about 0.75 % at 293 K for a compressive stress of 100 MPa. There is no effect of domain switching on conductivity except for the geometric effect. At 1073 K, the conductivity is simply proportional to the compressive strain.

A New Direct Updating Method for Undamped Structural Systems

A new numerical method for simultaneously updating mass and stiffness matrices based on incomplete modal measured data is presented. By using the Kronecker product, all the variables that are to be modified can be found out and then can be updated directly. The optimal approximation mass matrix and stiffness matrix which satisfy the required eigenvalue equation and orthogonality condition are found under the Frobenius norm sense. The physical configuration of the analytical model is preserved and the updated model will exactly reproduce the modal measured data. The numerical example seems to indicate that the method is quite accurate and efficient.

Biogas Production from Waste using Biofilm Reactor: Factor Analysis in Two Stages System

Factor analysis was applied to two stages biogas production from banana stem waste allowing a screening of the experimental variables second stage temperature (T), organic loading rates (OLR) and hydraulic retention times (HRT). Biogas production was found to be strongly influenced by all the above experimental variables. Results from factorial analysis have shown that all variables which were HRT, OLR and T have significant effect to biogas production. Increased in HRT and OLR could increased the biogas yield. The performance was tested under the conditions of various T (35oC-60oC), OLR (0.3 g TS/l.d–1.9 gTS/l.d), and HRT (3 d–15 d). Conditions for temperature, OLR and HRT in this study were based on the best range obtained from literature review.

The Effect of Carboxymethyl Cellulose on the Stability of Emulsions Stabilized by Whey Proteins under Digestion in vitro and in vivo

In vitro gastro-duodenal digestion model was used to investigate the changes of emulsions under digestion conditions. Oil in water emulsions stabilized by whey proteins (2%) and stabilized by whey proteins (2%) with addition of carboxymethyl cellulose (0.75%) as gelling agent of continuous phase were prepared at pH7. Both emulsions were destabilized under gastric conditions; however the protective role of carboxymethyl cellulose was indicated by recording delay of fat digestibility of this emulsion. In the presence of carboxymethyl cellulose whey proteins on the interfacial surface of droplets were more resistant to gastric degradation causing limited hydrolysis of fat due to the poor acceptability of lipids for the enzymes. Studies of emulsions using in vivo model supported results from in vitro studies. Lower content of triglycerides in blood serum and higher amount of fecal fat of rats were determined when rats were fed by diet containing emulsion made with whey proteins and carboxymethyl cellulose. 

Speaker Identification by Joint Statistical Characterization in the Log Gabor Wavelet Domain

Real world Speaker Identification (SI) application differs from ideal or laboratory conditions causing perturbations that leads to a mismatch between the training and testing environment and degrade the performance drastically. Many strategies have been adopted to cope with acoustical degradation; wavelet based Bayesian marginal model is one of them. But Bayesian marginal models cannot model the inter-scale statistical dependencies of different wavelet scales. Simple nonlinear estimators for wavelet based denoising assume that the wavelet coefficients in different scales are independent in nature. However wavelet coefficients have significant inter-scale dependency. This paper enhances this inter-scale dependency property by a Circularly Symmetric Probability Density Function (CS-PDF) related to the family of Spherically Invariant Random Processes (SIRPs) in Log Gabor Wavelet (LGW) domain and corresponding joint shrinkage estimator is derived by Maximum a Posteriori (MAP) estimator. A framework is proposed based on these to denoise speech signal for automatic speaker identification problems. The robustness of the proposed framework is tested for Text Independent Speaker Identification application on 100 speakers of POLYCOST and 100 speakers of YOHO speech database in three different noise environments. Experimental results show that the proposed estimator yields a higher improvement in identification accuracy compared to other estimators on popular Gaussian Mixture Model (GMM) based speaker model and Mel-Frequency Cepstral Coefficient (MFCC) features.

Application of Staining Intensity Correlation Analysis to Visualize Protein Colocalizationat a Cellular Level

Mutations of the telomeric copy of the survival motor neuron 1 (SMN1) gene cause spinal muscular atrophy. A deletion of the Eef1a2 gene leads to lower motor neuron degeneration in wasted mice. Indirect evidences have been shown that the eEF1A protein family may interact with SMN, and our previous study showed that abnormalities of neuromuscular junctions in wasted mice were similar to those of Smn mutant mice. To determine potential colocalization between SMN and tissue-specific translation elongation factor 1A2 (eEF1A2), an immunochemical analysis of HeLa cells transfected with the plasmid pcDNA3.1(+)C-hEEF1A2- myc and a new quantitative test of colocalization by intensity correlation analysis (ICA) was used to explore the association of SMN and eEF1A2. Here the results showed that eEF1A2 redistributed from the cytoplasm to the nucleus in response to serum and epidermal growth factor. In the cytoplasm, compelling evidence showed that staining for myc-tagged eEF1A2 varied in synchrony with that for SMN, consistent with the formation of a SMN-eEF1A2 complex in the cytoplasm of HeLa cells. These findings suggest that eEF1A2 may colocalize with SMN in the cytoplasm and may be a component of the SMN complex. However, the limitation of the ICA method is an inability to resolve colocalization in components of small organelles such as the nucleus.

Five Vital Factors Related to Employees’ Job Performance

The purpose of this research was to study five vital factors related to employees’ job performance. A total of 250 respondents were sampled from employees who worked at a public warehouse organization, Bangkok, Thailand. Samples were divided into two groups according to their work experience. The average working experience was about 9 years for group one and 28 years for group two. A questionnaire was utilized as a tool to collect data. Statistics utilized in this research included frequency, percentage, mean, standard deviation, t-test analysis, one way ANOVA, and Pearson Product-moment correlation coefficient. Data were analyzed by using Statistical Package for the Social Sciences. The findings disclosed that the majority of respondents were female between 23- 31 years old, single, and hold an undergraduate degree. The average income of respondents was less than 30,900 baht. The findings also revealed that the factors of organization chart awareness, job process and technology, internal environment, employee loyalty, and policy and management were ranked as medium level. The hypotheses testing revealed that difference in gender, age, and position had differences in terms of the awareness of organization chart, job process and technology, internal environment, employee loyalty, and policy and management in the same direction with low level.

Optimal Capacitor Placement in a Radial Distribution System using Plant Growth Simulation Algorithm

This paper presents a new and efficient approach for capacitor placement in radial distribution systems that determine the optimal locations and size of capacitor with an objective of improving the voltage profile and reduction of power loss. The solution methodology has two parts: in part one the loss sensitivity factors are used to select the candidate locations for the capacitor placement and in part two a new algorithm that employs Plant growth Simulation Algorithm (PGSA) is used to estimate the optimal size of capacitors at the optimal buses determined in part one. The main advantage of the proposed method is that it does not require any external control parameters. The other advantage is that it handles the objective function and the constraints separately, avoiding the trouble to determine the barrier factors. The proposed method is applied to 9, 34, and 85-bus radial distribution systems. The solutions obtained by the proposed method are compared with other methods. The proposed method has outperformed the other methods in terms of the quality of solution.

Multi Task Scheme to Monitor Multivariate Environments Using Artificial Neural Network

When an assignable cause(s) manifests itself to a multivariate process and the process shifts to an out-of-control condition, a root-cause analysis should be initiated by quality engineers to identify and eliminate the assignable cause(s) affected the process. A root-cause analysis in a multivariate process is more complex compared to a univariate process. In the case of a process involved several correlated variables an effective root-cause analysis can be only experienced when it is possible to identify the required knowledge including the out-of-control condition, the change point, and the variable(s) responsible to the out-of-control condition, all simultaneously. Although literature addresses different schemes to monitor multivariate processes, one can find few scientific reports focused on all the required knowledge. To the best of the author’s knowledge this is the first time that a multi task model based on artificial neural network (ANN) is reported to monitor all the required knowledge at the same time for a multivariate process with more than two correlated quality characteristics. The performance of the proposed scheme is evaluated numerically when different step shifts affect the mean vector. Average run length is used to investigate the performance of the proposed multi task model. The simulated results indicate the multi task scheme performs all the required knowledge effectively.

A Multistage Sulphidisation Flotation Procedure for a Low Grade Malachite Copper Ore

This study was carried out to develop a flotation procedure for an oxide copper ore from a Region in Central Africa for producing an 18% copper concentrate for downstream processing at maximum recovery from a 4% copper feed grade. The copper recoveries achieved from the test work were less than 50% despite changes in reagent conditions (multistage sulphidisation, use of RCA emulsion and mixture, use of AM 2, etc). The poor recoveries were attributed to the mineralogy of the ore from which copper silicates accounted for approximately 70% (mass) of the copper minerals in the ore. These can be complex and difficult to float using conventional flotation methods. Best results were obtained using basic sulphidisation procedures, a high flotation temperature and extended flotation residence time.

Physical, Textural and Sensory Properties of Noodles Supplemented with Tilapia Bone Flour (Tilapia nilotica)

Fishbone of Nile Tilapia (Tilapia nilotica), waste from the frozen Nile Tilapia fillet factory, is one of calcium sources. In order to increase fish bone powder value, this study aimed to investigate the effect of Tilapia bone flour (TBF) addition (5, 10, 15% by flour weight) on cooking quality, texture and sensory attributes of noodles. The results indicated that tensile strength, color value (a*) and water absorption of noodles significantly decreased (p£0.05) as the levels of TBF increased from 0-15%. While cooking loss, cooking time and color values (L* and b*) of noodles significantly increased (p£0.05). Sensory evaluation indicated that noodles with 5% TBF received the highest overall acceptability score.