Abstract: Introduction: Obesity is a major health risk issue in
the present day of life for one and all globally. Obesity is one of the
major concerns for public health according to recent increasing trends
in obesity-related diseases such as Type 2 diabetes. ( Kazuya,
1994).and hyperlipidemia, (Sakata,1990) .which are more prevalent
in Japanese adults with body mass index (BMI) values Z25 kg/m2.(
Japanese Ministry of Health and Welfare,1997). The purpose of the
study was to assess the effect of twelve weeks of brisk walking on
blood pressure and body mass index, anthropometric measurements
of obese males. Method: Thirty obese (BMI= above 30) males, aged
18 to 22 years, were selected from King Fahd University of
Petroleum & Minerals, Saudi Arabia. The subject-s height (cm) was
measured using a stadiometer and body mass (kg) was measured with
a electronic weighing machine. BMI was subsequently calculated
(kg/m2). The blood pressure was measured with standardized
sphygmomanometer in mm of Hg. All the measurements were taken
twice before and twice after the experimental period. The pre and
post anthropometric measurements of waist and hip circumference
were measured with the steel tape in cm. The subjects underwent
walking schedule two times in a week for 12 weeks. The 45 minute
sessions of brisk walking were undertaken at an average intensity of
65% to 85% of maximum HR (HRmax; calculated as 220-age).
Results & Discussion: Statistical findings revealed significant
changes from pre test to post test in case of both systolic blood
pressure and diastolic blood pressure in the walking group. Results
also showed significant decrease in their body mass index and
anthropometric measurements i.e. (waist & hip circumference).
Conclusion: It was concluded that twelve weeks brisk walking is
beneficial for lowering of blood pressure, body mass index, and
anthropometric circumference of obese males.
Abstract: Type 2 diabetes mellitus (T2DM) is a complex
metabolic disorder that characterized by the presence of high glucose
in blood that cause from insulin resistance and insufficiency due to
deterioration β-cell Langerhans functions. T2DM is commonly
caused by the combination of inherited genetic variations as well as
our own lifestyle. Metallothionein (MT) is a known cysteine-rich
protein responsible in helping zinc homeostasis which is important in
insulin signaling and secretion as well as protection our body from
reactive oxygen species (ROS). MT scavenged ROS and free
radicals in our body happen to be one of the reasons of T2DM and its
complications. The objective of this study was to investigate the
association of MT1A and MT2A polymorphisms between T2DM and
control subjects among Malay populations. This study involved 150
T2DM and 120 Healthy individuals of Malay ethnic with mixed
genders. The genomic DNA was extracted from buccal cells and
amplified for MT1A and MT2A loci; the 347bp and 238bp banding
patterns were respectively produced by mean of the Polymerase
Chain Reaction (PCR). The PCR products were digested with Mlucl
and Tsp451 restriction enzymes respectively and producing
fragments lengths of (158/189/347bp) and (103/135/238bp)
respectively. The ANOVA test was conducted and it shown that there
was a significant difference between diabetic and control subjects for
age, BMI, WHR, SBP, FPG, HBA1C, LDL, TG, TC and family
history with (P0.05). The genotype
frequency for AA, AG and GG of MT1A polymorphisms was 72.7%,
22.7% and 4.7% in cases and 15%, 55% and 30% in control
respectively. As for MT2A, genotype frequency of GG, GC and CC
was 42.7%, 27.3% and 30% in case and 5%, 40% and 55% for
control respectively. Both polymorphisms show significant difference
between two investigated groups with (P=0.000). The Post hoc test
was conducted and shows a significant difference between the
genotypes within each polymorphism (P=0. 000). The MT1A and
MT2A polymorphisms were believed to be the reliable molecular
markers to distinguish the T2DM subjects from healthy individuals in
Malay populations.