Nile Red, an Alternative Fluorescence Method for Quantification of Neutral Lipids in Microalgae

According to biodiesel from microalgae is an attractive fuel for several reasons such as renewable, biodegradable and environmental friendly. Thus, this study, green microalgae Scenedesmus acutus PPNK1 isolated from natural water, was selected based on high growth rates, easy cultivation and high lipid content. The Nile red fluorescence method has been successfully applied to the determination of lipids in S. acutus PPNK1. The combination of the method to the lipid composition in algal cells showed the yellow fluorescence under fluorescent microscope. Interestingly, maximum cell numbers and biomass concentration were obtained at 5.44´107 cells/mL and 1.60 g/L when it was cultivated in BG-11 medium while in case of BG-11 with nitrogen deprivation (N 0.25 g/L), accumulated lipid content in cells (44.67%) was achieved that was higher than that found in case of BG-11 medium at about 2 times (22.63%).

Real Time Detection, Tracking and Recognition of Medication Intake

In this paper, the detection and tracking of face, mouth, hands and medication bottles in the context of medication intake monitoring with a camera is presented. This is aimed at recognizing medication intake for elderly in their home setting to avoid an inappropriate use. Background subtraction is used to isolate moving objects, and then, skin and bottle segmentations are done in the RGB normalized color space. We use a minimum displacement distance criterion to track skin color regions and the R/G ratio to detect the mouth. The color-labeled medication bottles are simply tracked based on the color space distance to their mean color vector. For the recognition of medication intake, we propose a three-level hierarchal approach, which uses activity-patterns to recognize the normal medication intake activity. The proposed method was tested with three persons, with different medication intake scenarios, and gave an overall precision of over 98%.

Feasibility Study on Vanillin Production from Jatropha curcas Stem Using Steam Explosion as a Pretreatment

Jatropha curcas stem was analyzed for chemical compositions: 19.11% pentosan, 42.99% alphacellulose and 24.11% lignin based on dry weight of 100-g raw material. The condition to fractionate cellulose, hemicellulose and lignin in J. curcas stem using steam explosion was optimized. The procedure started from cutting J. curcas stem into small pieces and soaked in water for overnight. After that, they were steam exploded at 214 °C and 21 kg/cm2 for 5 min. The obtained hydrolysate contained 1.55 g/L ferulic acid which after that was used as substrate for vanillin production by Aspergillus niger and Pycnoporus cinnabarinus in one-step process. The maximum 0.65 g/L of vanillin were obtained with the conversion rate of 45.2% based on the initial ferulic acid.

Risk Factors in a Road Construction Site

The picture of a perfect road construction site is the one that utilizes conventional vertical road signs and a flagman to optimize the traffic flow with minimum hazel to the public. Former research has been carried out by Department of Occupational Safety and Health (DOSH) and Ministry of Works to further enhance smoothness in traffic operations and particularly in safety issues within work zones. This paper highlights on hazardous zones in a certain road construction or road maintenance site. Most cases show that the flagman falls into high risk of fatal accidents within work zone. Various measures have been taken by both the authorities and contractors to overcome such miseries, yet it-s impossible to eliminate the usage of a flagman since it is considered the best practice. With the implementation of new technologies in automating the traffic flow in road construction site, it is possible to eliminate the usage of a flagman. The intelligent traffic light system is designed to solve problems which contribute hazardous at road construction site and to be inline with the road safety regulation which is taken into granted.

In-situ Chemical Oxidation of Residual TCE by Permanganate in Epikarst

In-situ chemical oxidation (ISCO) has been widely used for source zone remediation of Dense Nonaqueous Phase Liquids (DNAPLs) in subsurface environments. DNAPL source zones for karst aquifers are generally located in epikarst where the DNAPL mass is trapped either in karst soil or at the regolith contact with carbonate bedrock. This study aims to investigate the performance of oxidation of residual trichloroethylene found in such environments by potassium permanganate. Batch and flow cell experiments were conducted to determine the kinetics and the mass removal rate of TCE. pH change, Cl production, TCE and MnO4 destruction were monitored routinely during experiments. Nonreactive tracer tests were also conducted prior and after the oxidation process to determine the influence of oxidation on flow conditions. The results show that oxidant consumption rate of the calcareous epikarst soil was significant and the oxidant demand was determined to be 20 g KMnO4/kg soil. Oxidation rate of residual TCE (1.26x10-3 s-1) was faster than the oxidant consumption rate of the soil (2.54 - 2.92x10-4 s-1) at only high oxidant concentrations (> 40 mM KMnO4). Half life of TCE oxidation ranged from 7.9 to 10.7 min. Although highly significant fraction of residual TCE mass in the system was destroyed by permanganate oxidation, TCE concentration in the effluent remained above its MCL. Flow interruption tests indicate that efficiency of ISCO was limited by the rate of TCE dissolution and the rate-limited desorption of TCE. The residence time and the initial concentration of the oxidant in the source zone also controlled the efficiency of ISCO in epikarst.

A Comparison of Exact and Heuristic Approaches to Capital Budgeting

This paper summarizes and compares approaches to solving the knapsack problem and its known application in capital budgeting. The first approach uses deterministic methods and can be applied to small-size tasks with a single constraint. We can also apply commercial software systems such as the GAMS modelling system. However, because of NP-completeness of the problem, more complex problem instances must be solved by means of heuristic techniques to achieve an approximation of the exact solution in a reasonable amount of time. We show the problem representation and parameter settings for a genetic algorithm framework.

The Classification Model for Hard Disk Drive Functional Tests under Sparse Data Conditions

This paper proposed classification models that would be used as a proxy for hard disk drive (HDD) functional test equitant which required approximately more than two weeks to perform the HDD status classification in either “Pass" or “Fail". These models were constructed by using committee network which consisted of a number of single neural networks. This paper also included the method to solve the problem of sparseness data in failed part, which was called “enforce learning method". Our results reveal that the constructed classification models with the proposed method could perform well in the sparse data conditions and thus the models, which used a few seconds for HDD classification, could be used to substitute the HDD functional tests.

Effect of Non-Newtonian Behaviour of Blood on Pulsatile Flows in Stenotic Arteries

In this paper, we study the pulsatile flow of blood through stenotic arteries. The inner layer of arterial walls is modeled as a porous medium and human blood is assumed as an incompressible fluid. A numerical algorithm based on the finite element method is developed to simulate the blood flow through both the lumen region and the porous wall. The algorithm is then applied to study the flow behaviour and to investigate the significance of the non-Newtonian effect.

Rapid Finite-Element Based Airport Pavement Moduli Solutions using Neural Networks

This paper describes the use of artificial neural networks (ANN) for predicting non-linear layer moduli of flexible airfield pavements subjected to new generation aircraft (NGA) loading, based on the deflection profiles obtained from Heavy Weight Deflectometer (HWD) test data. The HWD test is one of the most widely used tests for routinely assessing the structural integrity of airport pavements in a non-destructive manner. The elastic moduli of the individual pavement layers backcalculated from the HWD deflection profiles are effective indicators of layer condition and are used for estimating the pavement remaining life. HWD tests were periodically conducted at the Federal Aviation Administration-s (FAA-s) National Airport Pavement Test Facility (NAPTF) to monitor the effect of Boeing 777 (B777) and Beoing 747 (B747) test gear trafficking on the structural condition of flexible pavement sections. In this study, a multi-layer, feed-forward network which uses an error-backpropagation algorithm was trained to approximate the HWD backcalculation function. The synthetic database generated using an advanced non-linear pavement finite-element program was used to train the ANN to overcome the limitations associated with conventional pavement moduli backcalculation. The changes in ANN-based backcalculated pavement moduli with trafficking were used to compare the relative severity effects of the aircraft landing gears on the NAPTF test pavements.

Block Activity in Metric Neural Networks

The model of neural networks on the small-world topology, with metric (local and random connectivity) is investigated. The synaptic weights are random, driving the network towards a chaotic state for the neural activity. An ordered macroscopic neuron state is induced by a bias in the network connections. When the connections are mainly local, the network emulates a block-like structure. It is found that the topology and the bias compete to influence the network to evolve into a global or a block activity ordering, according to the initial conditions.

SUPAR: System for User-Centric Profiling of Association Rules in Streaming Data

With a surge of stream processing applications novel techniques are required for generation and analysis of association rules in streams. The traditional rule mining solutions cannot handle streams because they generally require multiple passes over the data and do not guarantee the results in a predictable, small time. Though researchers have been proposing algorithms for generation of rules from streams, there has not been much focus on their analysis. We propose Association rule profiling, a user centric process for analyzing association rules and attaching suitable profiles to them depending on their changing frequency behavior over a previous snapshot of time in a data stream. Association rule profiles provide insights into the changing nature of associations and can be used to characterize the associations. We discuss importance of characteristics such as predictability of linkages present in the data and propose metric to quantify it. We also show how association rule profiles can aid in generation of user specific, more understandable and actionable rules. The framework is implemented as SUPAR: System for Usercentric Profiling of Association Rules in streaming data. The proposed system offers following capabilities: i) Continuous monitoring of frequency of streaming item-sets and detection of significant changes therein for association rule profiling. ii) Computation of metrics for quantifying predictability of associations present in the data. iii) User-centric control of the characterization process: user can control the framework through a) constraint specification and b) non-interesting rule elimination.

File System-Based Data Protection Approach

As data to be stored in storage subsystems tremendously increases, data protection techniques have become more important than ever, to provide data availability and reliability. In this paper, we present the file system-based data protection (WOWSnap) that has been implemented using WORM (Write-Once-Read-Many) scheme. In the WOWSnap, once WORM files have been created, only the privileged read requests to them are allowed to protect data against any intentional/accidental intrusions. Furthermore, all WORM files are related to their protection cycle that is a time period during which WORM files should securely be protected. Once their protection cycle is expired, the WORM files are automatically moved to the general-purpose data section without any user interference. This prevents the WORM data section from being consumed by unnecessary files. We evaluated the performance of WOWSnap on Linux cluster.

Inferring the Dynamics of “Hidden“ Neurons from Electrophysiological Recordings

Statistical analysis of electrophysiological recordings obtained under, e.g. tactile, stimulation frequently suggests participation in the network dynamics of experimentally unobserved “hidden" neurons. Such interneurons making synapses to experimentally recorded neurons may strongly alter their dynamical responses to the stimuli. We propose a mathematical method that formalizes this possibility and provides an algorithm for inferring on the presence and dynamics of hidden neurons based on fitting of the experimental data to spike trains generated by the network model. The model makes use of Integrate and Fire neurons “chemically" coupled through exponentially decaying synaptic currents. We test the method on simulated data and also provide an example of its application to the experimental recording from the Dorsal Column Nuclei neurons of the rat under tactile stimulation of a hind limb.

The Role of Work Ethic in the Development of Singapore, South Korea, Malaysia, Japan and European Countries

Work ethic and labour productivity issues are extremely important for any society. It has been long proven by the global practice and various scholars that the country promoting the labour has always been way forward from the other countries. This paper studies the thoughts suggested by M.Weber, Confucius, Lee Kuan Yew, Mahathir Mohammad and other prominent thinkers concerning the issues of work ethics and labour productivity. The article analyzes why developed nations are way more advanced in their development compared to other nations.

Fatigue Crack Growth Behavior in Dissimilar Metal Weldment of Stainless Steel and Carbon Steel

Constant amplitude fatigue crack growth (FCG) tests were performed on dissimilar metal welded plates of Type 316L Stainless Steel (SS) and IS 2062 Grade A Carbon steel (CS). The plates were welded by TIG welding using SS E309 as electrode. FCG tests were carried on the Side Edge Notch Tension (SENT) specimens of 5 mm thickness, with crack initiator (notch) at base metal region (BM), weld metal region (WM) and heat affected zones (HAZ). The tests were performed at a test frequency of 10 Hz and at load ratios (R) of 0.1 & 0.6. FCG rate was found to increase with stress ratio for weld metals and base metals, where as in case of HAZ, FCG rates were almost equal at high ΔK. FCG rate of HAZ of stainless steel was found to be lowest at low and high ΔK. At intermediate ΔK, WM showed the lowest FCG rate. CS showed higher crack growth rate at all ΔK. However, the scatter band of data was found to be narrow. Fracture toughness (Kc) was found to vary in different locations of weldments. Kc was found lowest for the weldment and highest for HAZ of stainless steel. A novel method of characterizing the FCG behavior using an Infrared thermography (IRT) camera was attempted. By monitoring the temperature rise at the fast moving crack tip region, the amount of plastic deformation was estimated.

A survey Method and new design Lecture Chair for Complied Ergonomics Guideline at Classroom Building 2 Suranaree University of Technology, Thailand

The paper describes ergonomics problems trend of student at B5101 classroom building 2, Suranaree University of Technology. The objective to survey ergonomics problems and effect from use chairs for sitting in class room. The result from survey method 100 student they use lecture chair for sitting in classroom more than 2 hours/ day by RULA[1]. and Body discomfort survey[2]. The result from Body discomfort survey contribute fatigue problems at neck, lower back, upper back and right shoulder 2.93, 2.91, 2.33, 1.75 respectively and result from RULA contribute fatigue problems at neck, body and right upper arm 4.00, 3.75 and 3.00 respectively are consistent. After that the researcher provide improvement plan for design new chair support student fatigue reduction by prepare data of sample anthropometry and design ergonomics chair prototype 3 unit. Then sample 100 student trial to use new chair and evaluate again by RULA, Body discomfort and satisfaction. The result from trial new chair after improvement by RULA present fatigue reduction average of head and neck from 4.00 to 2.25 , body and trunk from 3.75 to 2.00 and arm force from 1.00 to 0.25 respectively. The result from trial new chair after improvement by Body discomfort present fatigue reduction average of lower back from 2.91 to 0.87, neck from 2.93 to 1.24, upper back 2.33 to 0.84 and right upper arm from 1.75 to 0.74. That statistical of RULA and Body discomfort survey present fatigue reduction after improvement significance with a confidence level of 95% (p-value 0.05). When analyzing the relationship of fatigue as part of the body by Chi – square test during RULA and Body discomfort that before and after improvements were consistent with the significant level of confidence 95% (p-value 0.05) . Moreover the students satisfaction result from trial with a new chair for 30 minutes [3]. 72 percent very satisfied of the folding of the secondary writing simple 66% the width of the writing plate, 64% the suitability of the writing plate, 62% of soft seat cushion and 61% easy to seat the chair.

Performance of a Connected Random Covered Energy Efficient Wireless Sensor Network

For the sensor network to operate successfully, the active nodes should maintain both sensing coverage and network connectivity. Furthermore, scheduling sleep intervals plays critical role for energy efficiency of wireless sensor networks. Traditional methods for sensor scheduling use either sensing coverage or network connectivity, but rarely both. In this paper, we use random scheduling for sensing coverage and then turn on extra sensor nodes, if necessary, for network connectivity. Simulation results have demonstrated that the number of extra nodes that is on with upper bound of around 9%, is small compared to the total number of deployed sensor nodes. Thus energy consumption for switching on extra sensor node is small.

Blind Channel Estimation Based on URV Decomposition Technique for Uplink of MC-CDMA

In this paper, we investigate a blind channel estimation method for Multi-carrier CDMA systems that use a subspace decomposition technique. This technique exploits the orthogonality property between the noise subspace and the received user codes to obtain channel of each user. In the past we used Singular Value Decomposition (SVD) technique but SVD have most computational complexity so in this paper use a new algorithm called URV Decomposition, which serve as an intermediary between the QR decomposition and SVD, replaced in SVD technique to track the noise space of the received data. Because of the URV decomposition has almost the same estimation performance as the SVD, but has less computational complexity.

The Residual Effects of Different Doses of Atrazine+Alachlor and Foramsulfuron on the Growth and Physiology of Rapeseed (Brassica napus L.)

A pot experiment was carried out under controlled conditions to evaluate the residual effects of different doses of atrazine+alachlor and foramsulfuron used in corn fields on the growth and physiology of rapeseed (Brassica napus L.). A split-plot experiment in CRD with 4 replications was used. The main plots consisted of herbicide type (atrazine+alachlor mixture and foramsulfuron) and the sub-plots were different residual doses of the herbicides (0, 1%, 5%, 10%, 20%, 40%, 50% and 100%). 7 cm diameter pots were filled with a virgin soil and seeds of rapeseed cv. Hayola were planted in them. The pots were kept under controlled conditions for 8 weeks after germination. At harvest, the growth parameters and the chlorophyll contents of the leaves were determined. The results showed that the growth of rapeseed plants was completely prevented at the highest residual doses of the herbicides (50 and 100 %). The growth parameters of rapeseed plants were affected by all doses of both types of the herbicide as compared to the controls. The residual effects of atrazine+alachlor mixture in reducing the growth parameters of rapeseed were more pronounced as compared to the residual effects of foramsulfuron alone.

Simulation and Analysis of the Shift Process for an Automatic Transmission

The automatic transmission (AT) is one of the most important components of many automobile transmission systems. The shift quality has a significant influence on the ride comfort of the vehicle. During the AT shift process, the joint elements such as the clutch and bands engage or disengage, linking sets of gears to create a fixed gear ratio. Since these ratios differ between gears in a fixed gear ratio transmission, the motion of the vehicle could change suddenly during the shift process if the joint elements are engaged or disengaged inappropriately, additionally impacting the entire transmission system and increasing the temperature of connect elements.The objective was to establish a system model for an AT powertrain using Matlab/Simulink. This paper further analyses the effect of varying hydraulic pressure and the associated impact on shift quality during both engagment and disengagement of the joint elements, proving that shift quality improvements could be achieved with appropriate hydraulic pressure control.